SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene25026

Length: 907 nt

Origin of reads: Biogeco1 v2 Uagpf v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene25026

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Probable glutathione S-transferase n=1 Tax=Picea mariana RepID=O65056_PICMA	-	-	78%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	81%
Blast2go	tau class glutathione s-transferase	-	-	82%

Source	InterPros	Term	Type	Identity
Sma3	Lipocalin	IPR002345	-	-
Sma3	Glutathione S-transferase, N-terminal	IPR004045	-	-
Sma3	Glutathione S-transferase, C-terminal	IPR004046	-	-
Sma3	Glutathione S-transferase, omega-class	IPR005442	-	-
Sma3	Glutathione S-transferase, C-terminal-like	IPR010987	-	-
Sma3		IPR012335	-	-
Sma3	Glutathione S-transferase/chloride channel, C-terminal	IPR017933	-	-
Sma3	Chromogranin, conserved site	IPR018054	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9P2I2

Fln msg: Overlapping hits, possible frame ERROR between 411 and 408, atg_distance in limit (1-15): atg_distance = 4, W2: There is no M at the beginning,

Fln protein: EACTEEEQVKLLGWLRSPFVTRVRIALALKGIQYQFIEDDAENKSQLLLQSNPVHKKVPVLIHNGKIVCESMIIVQYIDETWDTKAPNLLPKDPYDRAIARFWAAFVDDKLLLSIWGVVVGQGEKQQKAVEESLAxxxLLDDALRASSCSGNAYFGGVEIGFADIALGGLLVTIKAIQKVTNTVLVDPQKMPHLCAWIDRFSEIDLVKGILPDPVKVLERVSFMRAKFTSATAGN*

Protein Length: 237

Fln nts: GAGGCTTGTACAGAAGAGGAACAGGTGAAACTGTTAGGTTGGCTTCGCAGTCCCTTCGTAACGCGGGTTCGCATAGCACTTGCTCTGAAAGGGATCCAGTACCAGTTCATCGAAGACGACGCTGAAAATAAAAGCCAGCTGCTGCTGCAATCGAACCCTGTTCACAAGAAGGTTCCGGTGTTAATCCACAATGGAAAGATTGTTTGCGAGTCTATGATTATTGTGCAGTATATTGACGAGACGTGGGACACAAAGGCGCCGAATCTTCTACCCAAAGATCCATACGACCGAGCCATTGCCCGCTTCTGGGCTGCTTTTGTAGACGATAAGCTCCTTCTAAGTATTTGGGGAGTTGTGGTGGGGCAAGGTGAGAAGCAGCAAAAAGCGGTGGAAGAATCGTTGGCTxxxxxxxxxTTGTTAGACGACGCTCTGCGAGCGAGTAGCTGCTCGGGAAACGCGTATTTTGGAGGGGTTGAAATTGGCTTCGCTGATATCGCACTGGGAGGGTTGTTGGTAACTATCAAAGCCATCCAAAAGGTTACCAATACAGTTTTAGTGGATCCACAGAAGATGCCGCACTTATGTGCATGGATAGACCGATTCTCGGAAATCGATTTAGTGAAAGGAATTTTGCCAGATCCGGTCAAGGTATTGGAACGTGTATCCTTCATGAGAGCCAAATTTACCTCAGCAACGGCCGGCAATTAGGGAAGATCATTTAGGCTGGATAAAATTAGGGCCAATAATGTTAGAAGTAATGTGTGTAGTTATCATTCTCAGTAACTAGGCGTCAGTCTCATTCTTATGTAATGAGTTAGAGTCATTAGCTAGAAAGGTCCAAATCAGTGTGTAATTTAATATATATGTAATTTTTGAAATGAATAATAATATATGTAAAATTTGTAGTT

Fln Alignment:

Contig25026___EACTEEEQVKLLGWLRSPFVTRVRIALALKGIQYQFIEDDAENKSQLLLQSNPVHKKVPVLIHNGKIVCESMIIVQYIDETWDTKAPNLLPKDPYDRAIARFWAAFVDDKLLLSIWGVVVGQGEKQQKAVEESLANxLLLDDALRASSC-SGNAYFGGVEIGFADIALGGLLVTIKAIQKVTNTVLVDPQKMPHLCAWIDRFSEIDLVKGILPDPVKVLERVSFMRAKFTSATAGN
A9P2I2_______________EACGEEGQVKLLGASISPFVLRVRIALALKGIHYEFIEENLLNKSQLLLQSNPVHKKIPVLIHNGKPVCESMIIVQYIEEAWDSKAPNLMPKDPYDRAIARFWAAFVDDKLLPCVRGVFTGQGEQQQKAVEESVANxLVLEEALRTSLCFSGKPYFGGDEIGFVDIALGGMLAFVKALEKATNSVLIDVEKMPLLSAWMDRFCKADEVKEVMPDPAKLLEFIIAMKVRFASPPAAN

SNPs (tot: 25)

Position	A %	C %	G %	T %	Probability
609		87		12	0.992554
637	11		88		0.999952
671	13			86	0.999973
672	13			86	0.999973
674	85	14			0.999981
675			14	85	0.999981
691		84	15		0.999986
696		81		18	0.999994
699		81		18	0.999994
700			81	18	0.999994
703		81		18	0.999994
720		90		10	0.90298
726	11		88		0.923564
730	11		88		0.923564
742			88	11	0.923564
746	88		11		0.923564
752	11		88		0.923564
755	88		11		0.923564
760		11		88	0.923564
766	12		87		0.992554
776		62		37	1.0
781		12		87	0.992554
833		28		71	1.0
899			25	50	0.995533
902	66	33			0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain