SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene24448

Length: 1155 nt

Origin of reads: Biogeco1 v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene24448 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	NBS/LRR (Fragment) n=1 Tax=Pinus taeda RepID=Q8L8J3_PINTA	-	-	9.0e-38	41%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	48%
Blast2go	nbs lrr	-	-	2.10195e-44	67%

Source	InterPros	Term	Type	Identity
Sma3	Toll/interleukin-1 receptor homology (TIR) domain	IPR000157	-	-
Sma3	Leucine-rich repeat	IPR001611	-	-
Sma3	NB-ARC	IPR002182	-	-
Sma3	Immunoglobulin/major histocompatibility complex, conserved site	IPR003006	-	-
Sma3	AAA+ ATPase domain	IPR003593	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LMX8

Fln msg: Separated hits, possible frame ERROR between 546 and 615, your sequence is shorter than subject: 309 - 653

Fln protein: IRLSSTNPLHGFENLVDQFLQVCGGLPLALKVLGALLCGKPESYWDAKLNESKVQMKVSPDIKKRLKISYDALSEEEQKIFLDIAFFFIGESKTSAIAVWDASGWNGLLGLTDLEGKCLVEVDDKNVIHMHDQLRDLGRQIAEEETPRRLWRPTGDIDEWLHEYSSVLTEARGIWLLGERHxxxxxxxxxxxxxxxxxxxxxxxxLGRDVMNFQLVKTEGHLLDRILRVHSPNLSWLSWRHCPYTCLPSWITIKNLRVLKMEGAVLKTLWQGISQAPLQLQELDLSLCTQLERLPDLSAACRTSTAWT*

Protein Length: 310

Fln nts: ATACGCCTTTCGTCAACCAATCCACTCCATGGATTTGAAAATCTTGTAGATCAGTTTCTTCAAGTATGCGGTGGACTGCCTTTAGCTTTGAAAGTGCTTGGGGCACTTCTCTGTGGAAAACCTGAATCATATTGGGATGCTAAACTTAATGAATCAAAAGTTCAAATGAAAGTTTCACCTGATATAAAGAAGAGATTAAAAATCAGTTACGACGCACTCAGCGAAGAGGAGCAGAAGATCTTTTTAGATATCGCCTTTTTCTTCATAGGAGAAAGCAAAACAAGTGCCATAGCAGTTTGGGACGCGTCAGGCTGGAACGGTTTGCTGGGGCTTACAGATTTAGAGGGCAAATGTCTAGTTGAAGTAGATGATAAAAATGTGATACATATGCACGATCAACTGCGAGACCTGGGAAGACAGATTGCAGAGGAAGAAACGCCGCGCCGTCTTTGGCGTCCCACCGGGGACATTGATGAATGGTTGCACGAATACTCCTCTGTTTTAACGGAAGCACGCGGAATTTGGCTGCTCGGTGAGAGGCATAGxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTCTTGGTCGTGATGTCATGAATTTTCAACTTGTTAAGACTGAAGGACATCTGCTAGATCGTATTTTGCGGGTGCATTCGCCAAATCTCTCATGGCTTTCTTGGCGTCATTGTCCCTACACTTGTCTACCTTCGTGGATTACAATTAAGAATTTAAGGGTTTTGAAAATGGAGGGCGCTGTATTGAAAACATTATGGCAAGGCATATCGCAGGCACCTTTGCAGTTGCAGGAATTGGACCTGTCTTTGTGCACTCAATTGGAGAGGCTGCCAGATCTTTCGGCAGCTTGTCGAACCTCCACAGCTTGGACCTGAATTCGTGCTCTCAATTGGGAGTCTGCCATATTCTTTCGGCAGCTTGTCGAACCTCCACAGCCTGAACCTGCGTGAGTGCAAGAAATTGGAGAGGCTGCCAGATTCTTTCGGCAGGTTGTCGAACCTCCACAGCTTGGACCTGTTTGATTGCCTGAAATTTGGAGAGTCTACCAGATTCTTTCGGCAGCTTGTCGAACCTCCACAGCTTGAACCTGCTTGGGTGCGAGAA

Fln Alignment:

Contig24448___SSTNPLHGFENLVDQFLQVCXXXXXXXXXXXXXXXXKPESYWDAKLNESKVQMKVSPDIKKRLKISYDALSEEEQKIFLDIAFFFIGESKTSAIAVWDASGWNGLLGLTDLEGKCLVEVDDKNVIHMHDQLRDLGRQIAEEETPRRLWRPTGDIDEWLHEYSSVLTEARGIWLLGERHSxxxxxxxxxxxxxxxxxxxxxxxLGRDVMNFQLVKTEGHLLDRIL-RVHSPNLSWLSWRHCPYTCLPSWITIKNLRVLKMEGAVLKTL--WQGISQAPLQLQE
B8LMX8_______________SQAYPLAGFERLVDNFLHCCKGLPLSLKVFGALLYSKDISQWEDELES--LQQILPSDIHETLIISYRSLNKEEKEIFLDIACFFINQNRDAAISTWNGSGWRAKRGFLNLQDKSLVEVDGENCIHMHDHLRDLGRQVAASSSPLRLWRTENVIEDLLHQSSDI--TVRGIRMVHDEYSxxxxxxxxxxxxxxxxxxxxxxxLERILRRLQILHIEGSLLERILMKVKLPNLLWFHWEKCPHSSLPAWVPIKNLRVLQVSHRRLNTMSFGEGEPQASLQLPE

SNPs (tot: 28)

Position	A %	C %	G %	T %	Probability
249	66			33	0.997452
671			33	66	0.997452
673		33		66	1.0
683	33			66	0.997452
702	33		66		0.997452
751	33		33	33	0.997753
782	66		33		0.997452
800	66		33		0.997452
835		33	66		1.0
839	66			33	0.997452
854		33		66	1.0
859		66		33	0.997452
865	33	66			0.997452
868		66		33	0.997452
876		66	33		0.997452
884	33		66		0.997452
900	66		33		0.997452
901	66	33			0.997452
904	66			33	0.997452
933		14		85	0.993533
941	16		83		0.994512
944		33		66	1.0
949		50	50		1.0
970			25	75	0.996473
978		50		50	1.0
1000		50	50		1.0
1004		50		50	1.0
1024		50		50	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain