SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene22548

Length: 2836 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene22548
CTTCCACATCACAACTGAACTGACGTCATTTCATAGGAAAACGTAAGAAATTCATCGATAAGGAAGGACGGAGCAGAGTATGATTGAGTAGATAAGGACATCCGACTCCCAAAAAATCGTTTGGATTTTTTGAGATCAAGGGGATCTTCTCCACTATATATTCTCCGCTATCTTCTGAGATCGGGGAAGGGGCCGACTGTATTCTCTTCGTTTATGTGTTTGGAAGGCTTAATTGGGCTCCTGGAATTAAATTGTTTTACAGTCGAGGTGTGTGTGGGGAATCATGGTGAAGGTGGAAGTGACCATGGAGGTTGGAGCCGATGGAGTTGCCATAATCAAGATGACCAATCCGCCGGTCAATGCTCTGGCGGTAACAGTTCTTGCAGGCCTGAAAGAAAAATATGACGAAGCAATGAGGAGAAATGATGTGAAGGCCATTGTTGTCACTGGTAATGGAGGCAAATTCTCAGGTGGTTTTGACATCAACGTTCTCCAGGAAGTACAACGGACTGGGAACTTGTCGCATTTGGGTAGAGTTTCTGTTGATCTTATGATCAATACTATTGAAGAGGCTAAGAAACCTTCCGTAGCTGCTATTCAAGGGCTTGCGCTTGGTGGTGGATTAGAATTAGCAATGTCTTGTCATGCCCGTATTTCAACCCCAAAAGCGCAGCTTGGGTTGCCAGAACTTCAACTTGGGATTATTCCAGGATTTGGAGGTACGCAGCGCCTTCCTCGTCTTGTAGGGCTTCAAAAGGCGGTTGAAATGATGCTGTTATCAAAATCGATCATGTCAGAGGAGGGGAGTAAACTAGGTTTAATTGACGCAATTGTATCTCCGGGAGAGTTGATGGCCACAGCACGAATGTGGGCTTTAGATATTGCAGAACGGCGGAGACCCTGGATAATTAGTCTAAAAAGGACCGATAAAATTGAGTCACTAGGGGAAGCACGTGAAATTCTGAAATTTGCAAGGGCTCAGGCAACAAAGGCTGCCCCAAATCTGCCACATCCCCAACTCTGCCTAGATGTTATCGAGGAGGGTATTGTAGCAGGAGGCCTGGCAGGAGTTCTCAAGGAAGAAAAGGGATTTTATGATATTGTTCTCTCAGACACTGCAAAAGGGCTGGTACACTTCTTCTTTGGCCAGCGAGCAACTACAAAGGTTCCCGGAGTGACTGACTTAGGGCTAAAACCAAGACAAATTAAAAAGGTAGCAGTTCTTGGTGGAGGGTTAATGGGTTCAGGTATAGCTACATCTCTTGTTCTCAGTAATATTCCAGTCGTTCTGAAGGAGGTAAATGCTGGTTTTCTTCAGCAAGGTATCAATAGAATTTCAGCAAACCTGAAGAGTCGTGTAAAGAAAGGAAGCATGACACAAGAAAAGTTTGAGAAGACTATGTCTCTGATCACGGGAGTTGTAGATTATGCAGAATTCAGGAATGTCGATATGGTGATAGAGGCTGTAATAGAGAAAATTTCCTTGAAGCAAGAAATATTTAGTGACCTGGAAAAGATTTGTCCTGCACACTGCATCTTGGCCTCTAATACATCTACGATTGATCTCAATCTCATTGGTGCAAAAACACATGTTCAAGACCGTATAGTAGGGGCACATTTTTTCAGTCCCGCGCATGTGATGCCACTCCTTGAAATTGTTCGAACAGAGAAAACTTCACCTCAGATTATTGTTGACCTTATAAATGTTGGAAAGAATATAAAGAAAGTCCCTATAGTGGTGGGAAATTGCACCGGCTTTGCTGTCAATCGAATGTTCTTTCCATACACTCAATCGGCTCATATGTTGGCAATTCTTGGTGTAGATATTTATAGAATAGACAAAGTCATCACAAGTTTTGGAATGCCAATGGGTCCGTTCAGATTGCAAGATCTTGTTGGATATGAAGTATCTATTGAGGTTGGCAAGCAATTTGTTACAGCCTTTGGAGATCGAACCTTCAAGACACCACTAACCGAACTTATGATAGAAAATAAACGAGCTGGGGAAAAGAATGGTAAGGGCTACTATATATATGATGAGAAGAGGAGAGCTCGTCCAGCACCTGAGGTCAAAGAGATCATCGAACAAGCAAGAAAAATTTCTAAAATAATGCCTGGTGGAAAGCCTATTGTTGTCACTGACCGGGAAATATTGGAGATGATATTCTTCCCGGTTGTGAATGAGACATGCCGGGTCCTGGATGAAGGGATTGCGATTAAAGCATCAGATCTTGATGTTTCATCTGTCCTAGGGATGGGCTTCCCTGCATACAGAGGCGGGATAGTTTTTTGGGGAGATTCTGTTGGTGCAGGGCATATATACAATAGTCTTAAGAAGTGGTCTGAATCCTATGGTGGACTTTTCAAACCATCTGCTTATTTGGAAGAACGAGCTGCAAGAGGCATTCCCCTGAGTGCACCCGTTGCTGCTAAAAAAGTCGGATCGCGCCTGTAAGATGTACATGCACGAGAGCATTTTATTATCTAAGCAGCAAAGTTGAAAAACATTTCGCACGAGAGTCTTGGCCAGTAATATAGTTACTTTTCCTATAGTTACTTTTCCTAGGTGGGTCGAGTCTCTGAGCAAGGTCGAAGCTTATTTTGTGCCTGAGCGGATTGAAGGGGATTGAGAATTTTCTTAATAGATACATTCTCAGACTTTTATAGTTGTAGAAAGAGTTTTGAGATGCACCCCATGGACATATCCAACTTTTAATTACATATGCACAAAAAAATATGGATATGCTTAGCTTTTAATAAAGTGATATTTGGAGCTGAGAGAAGTTATAAAAAATGGATCTAATATTTCCTGTGAAGCTTTTTTATTTCTTTTCAC

Ace file of the UniGene sp_v2.0_unigene22548 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	[I] COG1250 3-hydroxyacyl-CoA dehydrogenase	-	-	39%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	93%
Blast2go	peroxisomal fatty acid beta-oxidation multifunctional protein	-	-	82%

Source	GOs	Term	Type	e value	Identity
Blast2go	catalytic activity	GO:0003824	Molecular Function	0.0	82%

Source	InterPros	Term	Type	Identity
Sma3	3-hydroxyacyl-CoA dehydrogenase, conserved site	IPR006180	-	-
Sma3	3-hydroxyacyl-CoA dehydrogenase, NAD binding	IPR006176	-	-
Sma3	3-hydroxyacyl-CoA dehydrogenase, C-terminal	IPR006108	-	-
Sma3	Crotonase, core	IPR001753	-	-
Sma3	Dehydrogenase, multihelical	IPR013328	-	-
Sma3	Enoyl-CoA hydratase/isomerase, conserved site	IPR018376	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LR51

Fln msg:

Fln protein: MVKVEVTMEVGADGVAIIKMTNPPVNALAVTVLAGLKEKYDEAMRRNDVKAIVVTGNGGKFSGGFDINVLQEVQRTGNLSHLGRVSVDLMINTIEEAKKPSVAAIQGLALGGGLELAMSCHARISTPKAQLGLPELQLGIIPGFGGTQRLPRLVGLQKAVEMMLLSKSIMSEEGSKLGLIDAIVSPGELMATARMWALDIAERRRPWIISLKRTDKIESLGEAREILKFARAQATKAAPNLPHPQLCLDVIEEGIVAGGLAGVLKEEKGFYDIVLSDTAKGLVHFFFGQRATTKVPGVTDLGLKPRQIKKVAVLGGGLMGSGIATSLVLSNIPVVLKEVNAGFLQQGINRISANLKSRVKKGSMTQEKFEKTMSLITGVVDYAEFRNVDMVIEAVIEKISLKQEIFSDLEKICPAHCILASNTSTIDLNLIGAKTHVQDRIVGAHFFSPAHVMPLLEIVRTEKTSPQIIVDLINVGKNIKKVPIVVGNCTGFAVNRMFFPYTQSAHMLAILGVDIYRIDKVITSFGMPMGPFRLQDLVGYEVSIEVGKQFVTAFGDRTFKTPLTELMIENKRAGEKNGKGYYIYDEKRRARPAPEVKEIIEQARKISKIMPGGKPIVVTDREILEMIFFPVVNETCRVLDEGIAIKASDLDVSSVLGMGFPAYRGGIVFWGDSVGAGHIYNSLKKWSESYGGLFKPSAYLEERAARGIPLSAPVAAKKVGSRL*

Protein Length: 725

Fln nts: CTTCCACATCACAACTGAACTGACGTCATTTCATAGGAAAACGTAAGAAATTCATCGATAAGGAAGGACGGAGCAGAGTATGATTGAGTAGATAAGGACATCCGACTCCCAAAAAATCGTTTGGATTTTTTGAGATCAAGGGGATCTTCTCCACTATATATTCTCCGCTATCTTCTGAGATCGGGGAAGGGGCCGACTGTATTCTCTTCGTTTATGTGTTTGGAAGGCTTAATTGGGCTCCTGGAATTAAATTGTTTTACAGTCGAGGTGTGTGTGGGGAATC_-_ATGGTGAAGGTGGAAGTGACCATGGAGGTTGGAGCCGATGGAGTTGCCATAATCAAGATGACCAATCCGCCGGTCAATGCTCTGGCGGTAACAGTTCTTGCAGGCCTGAAAGAAAAATATGACGAAGCAATGAGGAGAAATGATGTGAAGGCCATTGTTGTCACTGGTAATGGAGGCAAATTCTCAGGTGGTTTTGACATCAACGTTCTCCAGGAAGTACAACGGACTGGGAACTTGTCGCATTTGGGTAGAGTTTCTGTTGATCTTATGATCAATACTATTGAAGAGGCTAAGAAACCTTCCGTAGCTGCTATTCAAGGGCTTGCGCTTGGTGGTGGATTAGAATTAGCAATGTCTTGTCATGCCCGTATTTCAACCCCAAAAGCGCAGCTTGGGTTGCCAGAACTTCAACTTGGGATTATTCCAGGATTTGGAGGTACGCAGCGCCTTCCTCGTCTTGTAGGGCTTCAAAAGGCGGTTGAAATGATGCTGTTATCAAAATCGATCATGTCAGAGGAGGGGAGTAAACTAGGTTTAATTGACGCAATTGTATCTCCGGGAGAGTTGATGGCCACAGCACGAATGTGGGCTTTAGATATTGCAGAACGGCGGAGACCCTGGATAATTAGTCTAAAAAGGACCGATAAAATTGAGTCACTAGGGGAAGCACGTGAAATTCTGAAATTTGCAAGGGCTCAGGCAACAAAGGCTGCCCCAAATCTGCCACATCCCCAACTCTGCCTAGATGTTATCGAGGAGGGTATTGTAGCAGGAGGCCTGGCAGGAGTTCTCAAGGAAGAAAAGGGATTTTATGATATTGTTCTCTCAGACACTGCAAAAGGGCTGGTACACTTCTTCTTTGGCCAGCGAGCAACTACAAAGGTTCCCGGAGTGACTGACTTAGGGCTAAAACCAAGACAAATTAAAAAGGTAGCAGTTCTTGGTGGAGGGTTAATGGGTTCAGGTATAGCTACATCTCTTGTTCTCAGTAATATTCCAGTCGTTCTGAAGGAGGTAAATGCTGGTTTTCTTCAGCAAGGTATCAATAGAATTTCAGCAAACCTGAAGAGTCGTGTAAAGAAAGGAAGCATGACACAAGAAAAGTTTGAGAAGACTATGTCTCTGATCACGGGAGTTGTAGATTATGCAGAATTCAGGAATGTCGATATGGTGATAGAGGCTGTAATAGAGAAAATTTCCTTGAAGCAAGAAATATTTAGTGACCTGGAAAAGATTTGTCCTGCACACTGCATCTTGGCCTCTAATACATCTACGATTGATCTCAATCTCATTGGTGCAAAAACACATGTTCAAGACCGTATAGTAGGGGCACATTTTTTCAGTCCCGCGCATGTGATGCCACTCCTTGAAATTGTTCGAACAGAGAAAACTTCACCTCAGATTATTGTTGACCTTATAAATGTTGGAAAGAATATAAAGAAAGTCCCTATAGTGGTGGGAAATTGCACCGGCTTTGCTGTCAATCGAATGTTCTTTCCATACACTCAATCGGCTCATATGTTGGCAATTCTTGGTGTAGATATTTATAGAATAGACAAAGTCATCACAAGTTTTGGAATGCCAATGGGTCCGTTCAGATTGCAAGATCTTGTTGGATATGAAGTATCTATTGAGGTTGGCAAGCAATTTGTTACAGCCTTTGGAGATCGAACCTTCAAGACACCACTAACCGAACTTATGATAGAAAATAAACGAGCTGGGGAAAAGAATGGTAAGGGCTACTATATATATGATGAGAAGAGGAGAGCTCGTCCAGCACCTGAGGTCAAAGAGATCATCGAACAAGCAAGAAAAATTTCTAAAATAATGCCTGGTGGAAAGCCTATTGTTGTCACTGACCGGGAAATATTGGAGATGATATTCTTCCCGGTTGTGAATGAGACATGCCGGGTCCTGGATGAAGGGATTGCGATTAAAGCATCAGATCTTGATGTTTCATCTGTCCTAGGGATGGGCTTCCCTGCATACAGAGGCGGGATAGTTTTTTGGGGAGATTCTGTTGGTGCAGGGCATATATACAATAGTCTTAAGAAGTGGTCTGAATCCTATGGTGGACTTTTCAAACCATCTGCTTATTTGGAAGAACGAGCTGCAAGAGGCATTCCCCTGAGTGCACCCGTTGCTGCTAAAAAAGTCGGATCGCGCCTGTAA___GATGTACATGCACGAGAGCATTTTATTATCTAAGCAGCAAAGTTGAAAAACATTTCGCACGAGAGTCTTGGCCAGTAATATAGTTACTTTTCCTATAGTTACTTTTCCTAGGTGGGTCGAGTCTCTGAGCAAGGTCGAAGCTTATTTTGTGCCTGAGCGGATTGAAGGGGATTGAGAATTTTCTTAATAGATACATTCTCAGACTTTTATAGTTGTAGAAAGAGTTTTGAGATGCACCCCATGGACATATCCAACTTTTAATTACATATGCACAAAAAAATATGGATATGCTTAGCTTTTAATAAAGTGATATTTGGAGCTGAGAGAAGTTATAAAAAATGGATCTAATATTTCCTGTGAAGCTTTTTTATTTCTTTTCAC

Fln Alignment:

Contig22548___MVKVEVTMEVGADGVAIIKMTNPPVNALAVTVLAGLKEKYDEAMRRNDVKAIVVTGNGGKFSGGFDINVLQEVQRTGNLSHLGRVSVDLMINTIEEAKKPSVXXXXXXXXXXXXXXAMSCHARISTPKAQLGLPELQLGIIPGFGGTQRLPRLVGLQKAVEMMLLSKSIMSEEGSKLGLIDAIVSPGELMATARMWALDIAERRRPWIISLKRTDKIESLGEAREILKFARAQATKAAPNLPHPQLCLDVIEEGIVAGGLAGVLKEEKGFYDIVLSDTAKGLVHFFFGQRATTKVPGVTDLGLKPRQIKKVAVLGGGLMGSGIATSLVLSNIPVVLKEVNAGFLQQGINRISANLKSRVKKGSMTQEKFEKTMSLITGVVDYAEFRNVDMVIEAVIEKISLKQEIFSDLEKICPAHCILASNTSTIDLNLIGAKTHVQDRIVGAHFFSPAHVMPLLEIVRTEKTSPQIIVDLINVGKNIKKVPIVVGNCTGFAVNRMFFPYTQSAHMLAILGVDIYRIDKVITSFGMPMGPFRLQDLVGYEVSIEVGKQFVTAFGDRTFKTPLTELMIENKRAGEKNGKGYYIYDEKRRARPAPEVKEIIEQARKISKIMPGGKPIVVTDREILEMIFFPVVNETCRVLDEGIAIKASDLDVSSVLGMGFPAYRGGIVFWGDSVGAGHIYNSLKKWSESYGGLFKPSAYLEERAARGIPLSAPVAAKKVGSRL
B8LR51_______________MVKVDVSMEVGADGVAIIKMTNPPVNALAVTVLAGLKEKYDEAMRRNDVKAIVVTGNGGKFSGGFDINVIQEVQQTGNLSHLGRVSVDLMINTIEDAKKPSVAAIQGLALGGGLELAMSCHARISTPKALLGLPELQLGIFPGFGGTQRLPRLVGLQKAVEMMLLSKSIMSEEGNKLGLIDAVVSPGELMTTARMWALDIAERRRPWIISLKRTDKLESLGEAREILKFARAQAKKTAPNLPHPQLCLDVIEEGIVAGGLAGVLKEEKGFNDIVISDTAKGLVHFFFSQRATTKVPGVTDLGLKPRQIKKVAVLGGGLMGSGIATSLVLSNIPVVLKEVNAGFLQQGINRISANLKSRVKKGSMTEEKFEKTMSLITGVVDYAEFRNVDMVIEAVLEKISLKQEIFSDLEKICPAHCILATNTSTIDLNLIGAKTHVQDRIIGAHFFSPAHVMPLLEIVRTEKTSPQIIIDLINVGKNIKKVPVVVGNCTGFAVNRMFFPYTQAAHMLANLGVDIYRIDKVITSFGMPMGPFRLQDLVGYGVSLEVGKQFVTAFADRTFTSPLTQLMIENKRAGEKNGKGYYVYDEKRRARPAPEVKEIIEQSIKITKIMPGGKAVVVTDREILEMIFFPVVNEACRVLDEGIASKASDLDVSVVLGMGFPSYRGGIVFWADSVGAGHIYSSLKKWYESYGGLFKPCAYLEERAARGIPLSAPVAAKNFGSRL

SNPs (tot: 31)

Position	A %	C %	G %	T %	Probability
264	20		80		0.995493
1687		33		66	0.997452
1845		50		50	1.0
2467		55		44	1.0
2505		13		86	1.0
2561	11	88			1.0
2578	9			90	0.999842
2579	90		9		0.999842
2580	90			9	0.999842
2581	90		9		0.999842
2586			10	90	0.999898
2587		10		90	0.999898
2588		10		90	0.999898
2591			10	90	0.999898
2592			10	90	0.999898
2593		90		10	0.999898
2596		10	90		0.999898
2651		75		6	1.0
2652		87		12	0.999964
2653		75	12	12	0.999931
2654	12	75			0.999931
2655	12	6	75		0.999931
2656	75			6	1.0
2658	7	61	30		1.0
2659	38			61	1.0
2667	66		33		1.0
2669	58	33		8	1.0
2670	36		63		1.0
2673	36		63		1.0
2674	63	36			1.0
2689	14		85		0.993533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain