SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene21622

Length: 1723 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene21622 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	CC-NBS-LRR resistance-like protein [Pinus lambertiana]	-	-	2.0e-11	71%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	61%
Blast2go	protein	-	-	8.36575e-43	57%

Source	InterPros	Term	Type	Identity
Sma3	Leucine-rich repeat	IPR001611	-	-
Sma3	NB-ARC	IPR002182	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LQ70

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 204 aas, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 158 - 376

Fln protein: GHSRNRFSGPPWQLLSGPTHKESFIECGKTRTGYSLISALTGILVSKTLKSDSPDNRRSNPAPIKIIEAMSIPEALGETVGEETAAAHSA*PVGMEVATPLEALLWTMWKPTEGHDIMKTYKSVRSLWPVDEERVHLYHRNKSYETTGGRATHVFKK*

Protein Length: 159

Fln nts: ACATTGGAACCCTGCTAAATATATACATATGTTACAGCTTTGTAGCTGCATATTTAAAAGGATAAACGAGCATTCAAAGATGTCCAGGGCATTGCCCACTTCAAAAGATAATTAACAATTCAAATCGCCTCCAGGGCAATTTTATCCACGGCAATTTTATTAACGTGTGCTATGTTTAAGGCCAATGGACCTTCACATTCAATCTTGCTTAAAATTAATCAAATTTGATATACATCATGGCCTAAAATTACGGTTTACGGTTATTGACAACCATCATGGCCTATAATTACGGCTTCGTATATATAGACATACATACAGAGCTGAGGTTAGGCTTAAGGCTTAGATCATTGGGACAGAATCAGATTTTAGCACGAATCCTGACCTGCCCGTCGAAACTCGCCACAACAATACAACTTTCTCTCTGAGAAACCTCAAATCCAAAACCCTAGGCTAGCTGCAAATCAAATGCAATCACCTAAAAAATATAAAACCAAAATCATCTGACCTCGATAACCGATAAACTTACGGTGGCTGAGGTCTTCAAGAGCTCAAGCTGTTTGTTGCCTCTGTGGAGCAAATATTCGATGTAGGTGAAATCCTTTTTATATCTTCAAATACATGTCCAAGCTTACGAATGATCCACAGCAAAATGCGATGATGCTTCAACAAATGGGCGCAGATAATATGTGTGAGGGTGCTTTTGTCGAAGTTTCTTGTAATGATATTGGGATGGGAGTGTCGGATTCTGGGACCTCTGGATATTTTCCTTCAAGCTTTGGCCAAATACTCTGCAATTAGGCCAATTTTTGCGTCCAGCTGAGGCGGGATCTTCCTTTCCTTTGAATCAAAATCCAGTGATTCGAACACTTCTTTGGCACTCCAGCCGGCTTTTCTGAGAGAAACGAAGAGCAAGTCGACATGAAATGCGAAGCTTTTCAAAAGATTCTGGCTGTCCGAGCCTTTCTGAAAACAAGAAGCCGGGGTATTTATCATATCAGAGATCTCATATTCTTTCCAACCGCCATTCCTTAGCCTGGAGCCGATGTCTCCTAAATAACCTGCCAGAAATTTTCTGCTGAAGCAATTCCGGGCCTCCGGGAGAGGTCTTTCATCAAATCTTGGTTGTTTCTTTCCTGAATTTGCGATTTCGACATCGACTTTAACAGAGGAGAAAAGCCCTTCCGGAGGTGCGATGTTCAATACCTGTTTCTTTTCT_-_ATGAGGTCACTCCAGAAATCGATTCTCCGGGCCGCCTTGGCAACTCCTCTCTGGACCAACCCACAAGGAAAGTTTTATTGAATGCGGCAAAACTAGAACTGGGTATTCATTAATTTCTGCGCTAACTGGCATTCTCGTTTCCAAGACATTGAAATCAGACAGCCCAGATAACCGGAGATCGAATCCCGCGCCCATCAAAATAATAGAAGCCATGTCGATTCCAGAGGCATTGGGGGAAACAGTTGGGGAAGAGACTGCAGCAGCTCACTCCGCATGACCAGTGGGAATGGAAGTCGCGACACCACTGGAAGCACTTCTGTGGACCATGTGGAAGCCTACTGAGGGCCATGATATTATGAAAACCTACAAGTCCGTGAGATCACTGTGGCCTGTGGACGAAGAGCGCGTGCATCTGTATCACAGGAATAAGTCGTATGAAACCACTGGGGGAAGGGCTACCCATGTATTTAAGAAGTAAGCTGCGCGGTGATTCCCAAATTCGAAATT

Fln Alignment:

Contig21622___PWQLLSGPTHKESFIECGKTRTGYSLISALTGILVSKTLKSDSPDNRRSNPAPIKIIEAMSIPEALGETVGEETA---AAHSA*PVGMEVATPLEALLWTMWKPTEGHDIMKTYKSVRSLWPVDEERVHLYHRNKSYETTG
B8LQ70_______________PSQTLSGPTHNA--VECRKNRTGSSTLFALTRTLVSKALKSNSPDNGRSNPRPSKIIKAMSTPGVLEETIGEESVQQLTPHDQWEWNSRHHWKHWHGLWTMWKPTEGPDIVKTYKSVRSLWPVDEERVHLYHRNQWYETSG

SNPs (tot: 64)

Position	A %	C %	G %	T %	Probability
498	20		80		0.995493
502		80	20		0.995493
503		80	20		0.995493
541	20		80		0.995493
558		80		20	0.995493
579			25	75	0.996473
586		12		87	0.992554
591		87	12		0.992554
592	87		12		0.992554
600	87		12		0.992554
602		62	37		1.0
605	87			12	0.992554
607	12	75			0.975346
608	12			87	0.992554
609			12	87	0.992554
613	12			87	0.992554
615	87	12			0.992554
617	87			12	0.992554
618	37		62		1.0
619	87		12		0.992554
620	37	37	12	12	1.0
622	12			87	0.992554
623		87	12		0.992554
624	12			75	0.975346
625		87	12		0.992554
626	12	75	12		0.975346
628		12	87		0.992554
629	14		85		0.993533
633		85		14	0.993533
635	14	85			0.993533
636		14	85		0.993533
639	85	14			0.993533
640			14	85	0.993533
657			66	33	1.0
658			66	33	1.0
659		83	16		0.994512
662	16		83		0.994512
666		16	83		0.994512
667		16		83	0.994512
668	16			83	0.994512
670			16	83	0.994512
672		16		83	0.994512
674		16	83		0.994512
716			60	40	1.0
740	66	33			0.997452
766		33		66	1.0
785			33	66	1.0
788	33		66		1.0
796	33	66			0.997452
811		33		66	1.0
819		33		66	1.0
1486	88		11		0.923564
1488		11	88		0.923564
1489	88		11		0.923564
1494		11		88	0.923564
1495	88	11			0.923564
1497		88		11	0.923564
1498	88		11		0.923564
1499	88		11		0.923564
1500	88	11			0.923564
1502			11	88	0.923564
1504		11	88		0.923564
1507		11		88	0.923564
1509	88			11	0.923564

Back

Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain