SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene18337

Length: 962 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene18337 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Myo-inositol oxygenase n=1 Tax=Eucalyptus grandis RepID=B3VDZ0_EUCGR	-	-	80%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	79%
Blast2go	inositol oxygenase 1	-	-	89%

Source	ECs	Term	Type	e value	Identity
Blast2go	Inositol oxygenase.	EC:1.13.99.1	-	0.0	89%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Ascorbate and aldarate metabolism	00053		0.0	89%
Blast2go	Inositol phosphate metabolism	00562		0.0	89%

Source	GOs	Term	Type	e value	Identity
Blast2go	syncytium formation	GO:0006949	Biological Process	0.0	89%
Blast2go	inositol oxygenase activity	GO:0050113	Molecular Function	0.0	89%

Source	InterPros	Term	Type	e value	Identity
Sma3	Metal-dependent phosphohydrolase, HD domain	IPR003607	-	0.0	-
Sma3	Inositol oxygenase	IPR007828	-	0.0	-

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NU49

Fln msg: Separated hits, possible frame ERROR between 222 and 333, Distance to subject end: 22 aas, atg_distance in limit (1-15): atg_distance = 12, W2: There is no M at the beginning,

Fln protein: LRGPKLESSGTPQSSRLCGVPDDSGTLELDGGFDTPDSNAFGHAFRDFEKESDRRAGVQEFYRINHINQTYDYxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxVLSKREQYGKLDKAVMSIWECCELLNEFVDESDPDLDEPQIEHLLQTAEAIRKDYPNEDWLHLTGLIHDLGKVLLHPSFGEEPQWAVVGDTYPLGCAFDESIVHCEFFKENPDFYNSKYNTKYGVYSENCGLDNVILSWGHDEYMHMVAKMNNTTLPPEALFIIRFHSFYALHRAGAYTHLMNEEDKEMLEWVKIFNKYDLYSKSKVRID

Protein Length: 322

Fln nts: CTGCGGGGTCCCAAACTCGAATCGTCGGGGACCCCGCAGAGTTCCAGACTCTGCGGGGTCCCCGACGATTCTGGAACTCTTGAGTTGGATGGGGGTTTTGACACACCAGACAGCAATGCCTTTGGTCATGCTTTCAGAGATTTTGAAAAGGAAAGCGACAGACGGGCTGGCGTGCAAGAATTTTACAGAATTAATCACATCAACCAGACTTATGACTATGTxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxGGTATTAAGCAAACGTGAACAGTATGGTAAGCTTGACAAAGCTGTTATGAGCATCTGGGAATGCTGTGAACTTCTAAACGAGTTTGTAGATGAGAGTGACCCTGACCTTGATGAGCCTCAAATAGAACATCTGCTACAGACAGCAGAGGCTATCCGGAAAGATTACCCAAATGAAGATTGGCTTCATTTGACTGGCCTAATCCATGATCTGGGCAAGGTACTACTCCATCCTAGCTTTGGAGAGGAACCTCAATGGGCAGTTGTAGGTGACACTTATCCTTTGGGATGTGCTTTCGATGAATCCATAGTTCACTGCGAGTTTTTCAAGGAAAATCCAGATTTTTACAACTCAAAATACAACACAAAGTACGGGGTGTACTCTGAGAATTGTGGACTAGATAACGTTATTCTTTCCTGGGGTCATGATGAGTACATGCACATGGTGGCTAAAATGAATAATACAACATTGCCTCCAGAGGCTCTCTTTATCATAAGATTCCATTCTTTCTATGCTCTGCATCGTGCTGGTGCTTACACGCACTTGATGAATGAAGAAGACAAGGAGATGCTCGAGTGGGTTAAAATATTCAATAAATATGACTTGTACAGTAAGAGTAAGGTCAGAATTGAT

Fln Alignment:

Contig18337___PDDSGTLELDGGFDTPDSNAFGHAFRDFEKESDRRAGVQEFYRINHINQTYDYVxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxVLSKREQYGKLDKAVMSIWECCELLNEFVDESDPDLDEPQIEHLLQTAEAIRKDYPNEDWLHLTGLIHDLGKVLLHPSFGEEPQWAVVGDTYPLGCAFDESIVHCEFFKENPDFYNSKYNTKYGVYSENCGLDNVILSWGHDEYMHMVAKMNNTTLPPEALFIIRFHSFYALHRAGAYTHLMNEEDKEMLEWVKIFNKYDLYSKSKVRID
A9NU49_______________PSESDILQLDGGFVTPDSNAFGHSFRDYDKESERRAGVQEFYRINHINQTYEYVxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxVLSKREHYGKLDKAVMSIWECCELLNEFVDESDPDLDEPQIEHLLQTAEAIRKDYPNEDWLHLTGLIHDLGKVLLHPSFGEEPQWAAVGDTYPLGCAFDESIVHYEYFNENPDFYNPKYNTKYGVYSEKCGLENVIISWGHDEYMHMVAKMNKTTLPPAALFIIRFHSFYAMHRAGAYTHLMNDEDKEMLEWVKIFNKYDLYSKSKVRVD

SNPs (tot: 15)

Position	A %	C %	G %	T %	Probability
363	33	66			1.0
371	66		33		1.0
373		66	33		1.0
376	33	66			1.0
378	33	66			1.0
379	66		33		1.0
380		33		66	1.0
381		33		66	1.0
382	33		66		1.0
388	33			66	1.0
390		66	33		1.0
391	33			66	1.0
393			33	66	1.0
589	87		12		0.992554
719	25		75		0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain