SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene15041

Length: 2257 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene15041
TGAAATAGATACTGAATGTAGCAACTGTAGGGTAAAGCTGACAGGTAGCGAAGGAGACTCTCGACCTTCGACTGAATTCATCAAGCGTGTTACTTTTCAGTGTGGGGGGAAGGGGGGCGGGGAGGGCATTAAGAGCAAGTCTTTGCGTGAGTTGCTTGCTGAAGAAGAGGACGACGATTCTTTGGATTTCTCCGTTTGGAATCCCACTACAAATTTTGTAATTAGTAGTCAAAATACGCAACAGAGGACAGCAAAACCTCTTTCTAGAAGTACTGCCCCAGCTGCTGATGAGTCTAGATTGGCATTAGATGGCTCAGGGGAAACCAGTGAAGGTAATGGAGCAACTGATCAGATTTCAGATCCAGAGACGAGAAGGGGGACTTATTCTGATTCAGTACAGAAGCAGGGCAAAGGGATGTCTTTATTGAGTTCCAAATTAAGACAATCCTACAGTAAACGGAAGGAAAACCACAGAAAGTTGTTCCCATGGCGATATGATTGGGAGAAGGAACCGATAGAAGTAGAGAGAAAGATCGACGAAAGAATAAAACATAATGTACACAGATGTGATGCGTGTGCGGTTAATCATGTCTCTGTTCTAAAGGCTCTCTCTCGTGCATTAGAAGTTACAGAAGAAGCTTATCTTGAAATGACAGATAGGGTTCTTGATGAGAATCCAGAGCTTGCATTGATGGGCTCTTGTCTACTGGTGATGTTGATGAAGGACCAAGATGTGTATGTGATGAATGTTGGTGACAGTCGTGCTATTGTGGCACAGTTGAGTCCGGAGACAAATTCCAGTACAAAACAGCACTGTAGTCATCCGAAGGGAGATGGGCATGATAGAGTGGGTTCAAGGGAGGCCTTTGTAAGGTCAGAATTGGAGGATATAACAGAGGAACCTCCTGCAGATTCTGCACCAATTGAAGCCAAATGGGAACGATGACTCAGAATTGCCAGCTCCTGGAACAAAGTTGAGTGCATTACAGTTATCAACAGATCACAGTACCAGCATTGAAGAAGAAGTGATGAGAATTAAAGCAGAACATCCTGATGACAGCCAGTCTATTTTGAATGATCGAGTCAAAGGCCGTTTGAAAGTCACTCGTGCATTTGGAGCTGGATTTCTCAAGCAGCCTAAGTGGAACAATGCGCTCGTGACCTTGGCTGTCACTGATTATATTGGGACGGCTCCATACATCACTTGCATGCCTTCCCTTCGTCATCATAGACTTGGTCCAAATGATCAGTTTTTAGTCCTTTCGTCAGATGGTTTGTATCAGTATTTAAGCAATCAGGAAGTTGTTTCCCAGGTGGAGTTGTTTATGGCAAATAATCCTGATGGTGACCCTGCACAACATCTCATTGAAGAATTACTTGTTCGGGCAGCCAACAAAGCTGGTATGGACTTCTATGAATTGTTGGATATCCCTCAGGGAGATCGGCGGAAATATCATGATGATGTTTCTGTTATGGTAATAGCACTTGAGGGAAGAAGGATATGGAGGTGGAGGTCCTCTGGTAAGTATTAATGTGATCCTTGCACTGCAATTTTTTTGATGCTTTCACATGGCGATTTGGACACTACTTATTCATTCCTTGTCATTATGGTGAGTAAGTGAGGTTTGTTTTCTGTTGACTACAAGCTGATTTCATATGTATTCCCAGTAGGGCAAGACGTACACACTTCTTAGATTTTGGCCGTTTGCCAGTATCCTGCTTAATGGGTACTTAATGTTCATTGTAATTAACAATGATGCTAAGAGCAGAGCAAACTTCTTTCACCATGTATACCTGAGATATTGAGGCATCTTTAGAAATGCCTTGTGCAAAGATTAGTTGTTTATTTGGATTTGAAAGCTTCATTACAAAATGATTCATTTGCAACAAGGTGGAAACTGCTAAATAGGGGATTACTTCGGTCCTATAAGTATCTACAATCAACTATTCTGTAGAAAGCTGAAGTTCACAGATCCATTCCCGAGGTTAACCAGGGAGCCTTAGCACTGTACTGTTTTAGTTCATGAATTGGCACATTGTGTTGCGTTGTACAAAGATGTTTGTGAAAATTCAAGAGCATGAAGGCAGCATTTGTTATGAGCCAGGTTTAATATTTATCCAGACTTGGCTTGGTATGCTTTCAAAACTTTTGTTCGGGAGAAAAATCAAAATCGGCCTTTTATTGCTAGATCTGTCTCCAATAAGCAACGAAAGGATTATGCAGTTTTGTCTGAGGTCCAAATATAATTTCTATTTT

Ace file of the UniGene sp_v2.0_unigene15041 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Protein phosphatase 2c, putative n=1 Tax=Ricinus communis RepID=B9RN12_RICCO	-	-	69%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	35%
Blast2go	membrane associated protein phosphatase 2c	-	-	82%

Source	GOs	Term	Type	Identity
Blast2go	regulation of transcription, DNA-dependent	GO:0006355	Biological Process	82%
Blast2go	maintenance of meristem identity	GO:0010074	Biological Process	82%
Blast2go	regulation of meristem structural organization	GO:0009934	Biological Process	82%
Blast2go	phospholipid binding	GO:0005543	Molecular Function	82%
Blast2go	protein serine/threonine phosphatase activity	GO:0004722	Molecular Function	82%
Blast2go	plasma membrane	GO:0005886	Cellular Component	82%

Source	InterPros	Term	Type	Identity
Sma3	Protein phosphatase 2C, manganese/magnesium aspartate binding site	IPR000222	-	-
Sma3	Protein phosphatase 2C-like	IPR001932	-	-
Sma3		IPR014045	-	-
Sma3	Protein phosphatase 2C	IPR015655	-	-
Sma3	WD40 repeat, conserved site	IPR019775	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LQT5

Fln msg: query STOP codon is far from subject stop. Distance to subject end: 26 aas, W1: There is no M at the beginning,

Fln protein: QRNLLQILHQLKPNGNDDSELPAPGTKLSALQLSTDHSTSIEEEVMRIKAEHPDDSQSILNDRVKGRLKVTRAFGAGFLKQPKWNNALVTLAVTDYIGTAPYITCMPSLRHHRLGPNDQFLVLSSDGLYQYLSNQEVVSQVELFMANNPDGDPAQHLIEELLVRAANKAGMDFYELLDIPQGDRRKYHDDVSVMVIALEGRRIWRWRSSGKY*

Protein Length: 214

Fln nts: TGAAATAGATACTGAATGTAGCAACTGTAGGGTAAAGCTGACAGGTAGCGAAGGAGACTCTCGACCTTCGACTGAATTCATCAAGCGTGTTACTTTTCAGTGTGtcagGAAGGtcaGCgGtGAGGGCATTAAGAGCAAGTCTTTGCGTGAGTTGCTTGCTGAAGAAGAGGACGACGATTCTTTGGATTTCTCCGTTTGGAATCCCACTACAAATTTTGTAATTAGTAGTCAAAATACGCAACAGAGGACAGCAAAACCTCTTTCTAGAAGTACTGCCCCAGCTGCTGATGAGTCTAGATTGGCATTAGATGGCTCAGGGGAAACCAGTGAAGGTAATGGAGCAACTGATCAGATTTCAGATCCAGAGACGAGAAGGGGGACTTATTCTGATTCAGTACAGAAGCAGGGCAAAGGGATGTCTTTATTGAGTTCCAAATTAAGACAATCCTACAGTAAACGGAAGGAAAACCACAGAAAGTTGTTCCCATGGCGATATGATTGGGAGAAGGAACCGATAGAAGTAGAGAGAAAGATCGACGAAAGAATAAAACATAATGTACACAGATGTGATGCGTGTGCGGTTAATCATGTCTCTGTTCTAAAGGCTCTCTCTCGTGCATTAGAAGTTACAGAAGAAGCTTATCTTGAAATGACAGATAGGGTTCTTGATGAGAATCCAGAGCTTGCATTGATGGGCTCTTGTCTACTGGTGATGTTGATGAAGGACCAAGATGTGTATGTGATGAATGTTGGTGACAGTCGTGCTATTGTGGCACAGTTGAGTCCGGAGACAAATTCCAGTACAAAACAGCACTGTAGTCATCCGAAGGGAGATGGGCATGATAGAGTGGGTTCAAGGGAGGCCTTTGTAAGGTCAGAATTGGAGGATATAACAGAGGAACCTCCTGCAGATTCTGCACCAATTGAAGCCAAATGGGAACGATGACTCAGAATTGCCAGCTCCTGGAACAAAGTTGAGTGCATTACAGTTATCAACAGATCACAGTACCAGCATTGAAGAAGAAGTGATGAGAATTAAAGCAGAACATCCTGATGACAGCCAGTCTATTTTGAATGATCGAGTCAAAGGCCGTTTGAAAGTCACTCGTGCATTTGGAGCTGGATTTCTCAAGCAGCCTAAGTGGAACAATGCGCTCGTGACCTTGGCTGTCACTGATTATATTGGGACGGCTCCATACATCACTTGCATGCCTTCCCTTCGTCATCATAGACTTGGTCCAAATGATCAGTTTTTAGTCCTTTCGTCAGATGGTTTGTATCAGTATTTAAGCAATCAGGAAGTTGTTTCCCAGGTGGAGTTGTTTATGGCAAATAATCCTGATGGTGACCCTGCACAACATCTCATTGAAGAATTACTTGTTCGGGCAGCCAACAAAGCTGGTATGGACTTCTATGAATTGTTGGATATCCCTCAGGGAGATCGGCGGAAATATCATGATGATGTTTCTGTTATGGTAATAGCACTTGAGGGAAGAAGGATATGGAGGTGGAGGTCCTCTGGTAAGTATTAA___TGTGATCCTTGCACTGCAATTTTTTTGATGCTTTCACATGGCGATTTGGACACTACTTATTCATTCCTTGTCATTATGGTGAGTAAGTGAGGTTTGTTTTCTGTTGACTACAAGCTGATTTCATATGTATTCCCAGTAGGGCAAGACGTACACACTTCTTAGATTTTGGCCGTTTGCCAGTATCCTGCTTAATGGGTACTTAATGTTCATTGTAATTAACAATGATGCTAAGAGCAGAGCAAACTTCTTTCACCATGTATACCTGAGATATTGAGGCATCTTTAGAAATGCCTTGTGCAAAGATTAGTTGTTTATTTGGATTTGAAAGCTTCATTACAAAATGATTCATTTGCAACAAGGTGGAAACTGCTAAATAGGGGATTACTTCGGTCCTATAAGTATCTACAATCAACTATTCTGTAGAAAGCTGAAGTTCACAGATCCATTCCCGAGGTTAACCAGGGAGCCTTAGCACTGTACTGTTTTAGTTCATGAATTGGCACATTGTGTTGCGTTGTACAAAGATGTTTGTGAAAATTCAAGAGCATGAAGGCAGCATTTGTTATGAGCCAGGTTTAATATTTATCCAGACTTGGCTTGGTATGCTTTCAAAACTTTTGTTCGGGAGAAAAATCAAAATCGGCCTTTTATTGCTAGATCTGTCTCCAATAAGCAACGAAAGGATTATGCAGTTTTGTCTGAGGTCCAAATATAATTTCTATTTT

Fln Alignment:

Contig15041___LQLSTDHSTSIEEEVMRIKAEHPDDSQSILND----RVKGRLKVTRAFGAGFLKQPKWNNALV--TLAVTDYIGTAPYITCMPSLRHHRLGPNDQFLVLSSDGLYQYLSNQEVVSQVELFMANNPDGDPAQHLIEELLVRAANKAGMDFYELLDIPQGDRRKYHDDVSVMVIALEGRRIWRWRSSG
B8LQT5_______________MQLSTEHNAGIEAVRQELQSLHPDDPRIVVLKHGVWRVKGIIQVSRSIGDVYLKKPEFNREPLYQKFRLSEPL-RGPILTAEPSINVHTLQPHDQFIIFASDGLWEHLSNQEAVDIVH----NHPHAGSARRLVKAALQEAAKKREMRYSDLKKIDRGVRRHFHDDITVIVVFLDHSLISKFSSRG

SNPs (tot: 49)

Position	A %	C %	G %	T %	Probability
254		75		25	0.996473
583		80	20		0.995493
584	20			80	0.995493
585		20		80	0.995493
586	20		80		0.995493
587		80	20		0.995493
592	20	80			0.995493
593			20	80	0.995493
595			80	20	0.995493
596		20	80		0.995493
598	80	20			0.995493
599			20	80	0.995493
602	80		20		0.995493
604	75		25		0.996473
605	25			75	0.996473
607	75			25	0.996473
608	75		25		0.996473
616		25		75	0.996473
617	25		75		0.996473
618	25			75	0.996473
619	75		25		0.996473
624	75	25			0.996473
625		75	25		0.996473
1770	83			16	0.994512
1800	20		80		0.995493
1804		20		80	0.995493
1805		80	20		0.995493
1806		80		20	0.995493
1809		80	20		0.995493
1811	20		80		0.995493
1919		33		66	0.997452
1920		33	66		0.997452
1923	33	66			0.997452
2144		75		25	0.996473
2145		75	25		0.996473
2146	25	75			0.996473
2149		66	33		0.997452
2150	33	66			0.997452
2152	33			66	0.997452
2155		33		66	0.997452
2156	33			66	0.997452
2157	33	66			0.997452
2158		66	33		0.997452
2159		66	33		0.997452
2163	33			66	0.997452
2164		33		66	0.997452
2165			33	66	0.997452
2166	33			66	0.997452
2168	66		33		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain