SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene14365

Length: 1259 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene14365 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Pollen major allergen No.121 isoform 1 n=1 Tax=Cryptomeria japonica RepID=Q588C0_CRYJA	-	-	68%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	89%
Blast2go	xyloglucan endotransglucosylase hydrolase	-	-	79%

Source	ECs	Term	Type	e value	Identity
Blast2go	Hydrolases, Glycosylases, Glycosidases, i.e. enzymes hydrolyzing O- and S-glycosyl compounds.	EC:3.2.1.-	-	0.0	79%

Source	KEGGs	Term	Type	Identity
Blast2go	Ascorbate and aldarate metabolism	00053		79%
Blast2go	Ubiquinone and other terpenoid-quinone biosynthesis	00130		79%
Blast2go	Amino sugar and nucleotide sugar metabolism	00520		79%
Blast2go	Glycosaminoglycan degradation	00531		79%
Blast2go	Metabolic pathways	01100		79%
Blast2go	Xyloglucan:xyloglucosyl transferase.	EC:2.4.1.207	-	79%

Source	GOs	Term	Type	Identity
Blast2go	cellular glucan metabolic process	GO:0006073	Biological Process	79%
Blast2go	hydrolase activity, hydrolyzing O-glycosyl compounds	GO:0004553	Molecular Function	79%
Blast2go	xyloglucan:xyloglucosyl transferase activity	GO:0016762	Molecular Function	79%
Blast2go	apoplast	GO:0048046	Cellular Component	79%
Blast2go	cell wall	GO:0005618	Cellular Component	79%

Source	InterPros	Term	Type	Identity
Sma3	Glycoside hydrolase, family 16	IPR000757	-	-
Sma3	Glycoside hydrolase, family 16, active site	IPR008263	-	-
Sma3	Beta-glucanase	IPR008264	-	-
Sma3	Xyloglucan endo-transglycosylase, C-terminal	IPR010713	-	-
Sma3	Concanavalin A-like lectin/glucanase, subgroup	IPR013320	-	-
Sma3	Xyloglucan endotransglucosylase/hydrolase	IPR016455	-	-
Sma3	Carbohydrate kinase, FGGY, conserved site	IPR018483	-	-
Sma3	DNA topoisomerase, type IIA, conserved site	IPR018522	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5AED5

Fln msg: Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 249 - 274

Fln protein: CRHWGNDRAKIIDNGQQLQLTLDRSSGCGIQSKQEYLFAKIDIQIKLVPGNSAGTVTTFYLSSQGPKHDEIDFEFLGNLSGDPYILHTNVFAQGLGGREQQFYLWFDPTLDFHTYSVLWTSNQIIFSVDGSPVRVFKNRETELGKVDSNYHYPKTQAMRVYSSLWNADDWATRGGLVKTDWTKAPFVATFRNFNAAATSSFDAAAEEVALESNQEQRQRLQWVRKNYMIYDYCADTKRFPQGPPPECK*

Protein Length: 250

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTATCAACATCGACGCTCAAAATATATATATATATTCTCTGCACACATTTATTCTCATTTCTTGAACTGCACCTCTGCATCGCAAACTCTTTTCCCAATCCTTAATCGGCATGGACACACCTCTTCACCTCCTCTTTCTTTTATTAGCCTCCTCGGCAGCTCGTGCTTCTGCAAATTTCTACAATGATGTCGACATTGGGGTAATGATCGTGCTAAAATCATTGACAATGGACAACAGTTGCAGCTTACTCTTGACCGCTCTTCTGGTTGTGGTATTCAATCCAAGCAAGAGTATCTATTTGCCAAGATTGATATCCAAATCAAGTTGGTACCGGGCAACTCTGCAGGCACAGTCACTACTTTTTATCTATCATCTCAAGGTCCCAAACACGACGAAATAGATTTCGAATTTCTGGGCAACCTCTCTGGAGATCCTTATATTTTGCACACTAATGTCTTTGCTCAAGGCCTTGGTGGGCGTGAACAACAATTCTACTTGTGGTTCGACCCAACCCTGGATTTCCACACTTACTCCGTGCTCTGGACATCAAACCAAATCATATTTTCTGTAGACGGGAGTCCTGTTCGGGTGTTTAAGAACAGGGAGACAGAGTTGGGCAAAGTGGATAGCAATTATCATTATCCCAAAACCCAAGCAATGAGGGTCTACTCCAGCCTTTGGAACGCAGATGATTGGGCAACCAGGGGTGGGCTTGTGAAGACCGACTGGACTAAAGCTCCCTTTGTTGCAACCTTCCGCAATTTCAATGCTGCCGCTACTTCTTCTTTTGACGCCGCCGCAGAGGAGGTGGCTTTGGAATCGAACCAAGAACAGAGGCAGAGGCTCCAGTGGGTACGAAAGAACTACATGATCTACGATTATTGTGCAGACACCAAGAGATTCCCCCAGGGACCGCCTCCCGAATGCAAATAA___AACCTCAATCCTTTGAATTCAGAGAATGAACTCTGAATACTTTCCTTCCAAGTATTCTCATTCATTTCTATTACATTAATTCTTTCATTCTTTCTCAGACACACATGTAAAATATTAGAGGGTATCAATCATTTCATTTTGGTACTGGTATACAAAAGCGGGATTCTTTTGATGATTCATCAATGGCTACAGCGTTTCCTTTTTGGTTACCGCATGTTCGCGTTTTACTCTTTTCTGAGAGACACACATACAAAGGCATTGATATATATATATATTTGACAAAATTTAGGATTAACACAC

Fln Alignment:

Contig14365___WGNDRAKIIDNGQQLQLTLDRSSGCGIQSKQEYLFAKIDIQIKLVPGNSAGTVTTFYLSSQGPKHDEIDFEFLGNLSGDPYILHTNVFAQGLGGREQQFYLWFDPTLDFHTYSVLWTSNQIIFSVDGSPVRVFKNRETELGKVDSNYHYPKTQAMRVYSSLWNADDWATRGGLVKTDWTKAPFVATFRXXXXXXXXXXXXXAEEVALESNQEQRQRLQWVRKNYMIYDYCADTKRFPQGPPPECK
D5AED5_______________WGDDRAKILDNGQQLQLTLDRTSGCGIQSKQEYLFAKIDMQIKLIPGNSAGTVTTYYLSSQGPKHDEIDFEFLGNLSGDPYIMHTNVFAQGLGNREQQFYLWFDPTLDFHTYSVLWTSNQIIFSVDGTPVRVFKNRETELGNVDTNYHYPKNQAMRIYSTLWNADDWATRGGLVKTDWTKAPFVASFRNFNAATSSSSDSAAEE-ALDSNQE--QRLQWVRKNYMIYNYCADTKRFPQGLPPECK

SNPs (tot: 18)

Position	A %	C %	G %	T %	Probability
62		66		33	0.997452
306			14	85	0.993533
307		14		85	0.993533
309	14	85			0.993533
713	4		95		0.995482
937	95		5		0.997749
960	93			6	0.999364
999		11		88	0.923564
1000	12		87		0.992554
1001	12			87	0.992554
1002			12	87	0.992554
1007	83		16		0.994512
1008	16			83	0.994512
1009		16		83	0.994512
1020		25		75	0.996473
1165		25	75		0.996473
1171		25		75	0.996473
1195	16		83		0.994512

SSRs (tot: 2)

Start position	End position	Sequence	Length
26	39	AT AT AT AT AT AT AT	14
1200	1213	AT AT AT AT AT AT AT	14

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain