SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene13990

Length: 907 nt

Origin of reads: Biogeco1 v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene13990 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	respiratory burst oxidase homolog [Nicotiana benthamiana]	-	-	4.0e-31	50%
FL-Next	sp=Respiratory burst oxidase homolog protein A; Solanum tuberosum (Potato).	-	-	0.0	50%
Blast2go	respiratory burst	-	-	1.12912e-33	67%

Source	ECs	Term	Type	e value	Identity
Blast2go	Oxidoreductases, Acting on NADH or NADPH, With a oxygen as acceptor.	EC:1.6.3.-	-	1.12912e-33	67%

Source	GOs	Term	Type	e value	Identity
Blast2go	metal ion binding	GO:0046872	Molecular Function	1.12912e-33	67%
Blast2go	oxidoreductase activity, acting on NADH or NADPH, oxygen as acceptor	GO:0050664	Molecular Function	1.12912e-33	67%
Blast2go	membrane	GO:0016020	Cellular Component	1.12912e-33	67%

Source	InterPros	Term	Type	Identity
Sma3	Cytochrome b245, heavy chain	IPR000778	-	-
Sma3	Calcium-binding EF-hand	IPR002048	-	-
Sma3	EF-hand-like domain	IPR011992	-	-
Sma3	FAD-binding 8	IPR013112	-	-
Sma3	Ferric reductase, NAD binding	IPR013121	-	-
Sma3	Flavoprotein transmembrane component	IPR013130	-	-
Sma3	NADPH oxidase Respiratory burst	IPR013623	-	-
Sma3	Ferredoxin reductase-type FAD-binding domain	IPR017927	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-
Sma3	EF-hand	IPR018248	-	-
Sma3	EF-HAND 2	IPR018249	-	-

Full-Lengther Next Prediction

Fln status: Internal

Fln database: sp_plants

Fln subject: Q948U0

Fln msg: Separated hits, possible frame ERROR between 734 and 749, Unexpected stop codon in the beginning of your sequence, Distance to subject end: 637 aas, your sequence is shorter than subject: 302 - 963

Fln protein: VRTHFASV*LRNCAWLQFTLEDEGLFFRNDDAGVWERRRGGGIQHGRKFWRQKLYSLET*FLRRSIAVAGQRCGGRCNAGCSHGRCCFDERE*G*GKKIDFQFHHGSLLAAF*TVFKKWWYLDYSDCSLDVFSRPLD*WK***RTKKPRLEKSLSVAEHAILGLRFINRATCDKDLDKLWAEVECRFNKLALPNYLLDRSDFAECIGMEESKEFALQLFDALSRRRARGRHLKSISKDELHEYxxxxxxNRSFESRIQIFFDLCDKDSDGRISEAEVREVILLSASANRLSILQEQADEICS

Protein Length: 303

Fln nts: CGGTGAGGACTCATTTTGCATCCGTTTGATTACGCAATTGCGCTTGGCTGCAATTTACGCTGGAGGACGAGGGTTTGTTCTTTAGAAATGATGATGCTGGGGTATGGGAGAGAAGGAGGGGTGGTGGCATTCAACATGGACGAAAATTCTGGCGGCAAAAGCTTTACTCCTTGGAAACCTAATTCCTTAGGCGATCTATTGCAGTGGCCGGTCAAAGATGTGGCGGAAGATGTAACGCTGGATGTTCTCATGGACGCTGTTGTTTTGACGAGCGCGAATGAGGCTGAGGAAAGAAGATCGATTTCCAATTCCACCATGGAAGCCTTCTCGCAGCATTCTAGACGGTCTTCAAGAAGTGGTGGTACCTTGACTACTCGGATTGTTCCCTTGACGTTTTCTCCCGACCTTTGGATTGATGGAAGTAATAATGAAGAACAAAGAAGCCTCGGCTCGAGAAGAGCTTATCCGTGGCCGAGCATGCAATCCTGGGACTCAGATTCATCAACAGGGCAACCTGTGACAAAGACTTGGATAAACTGTGGGCGGAGGTCGAATGCCGTTTCAACAAGCTTGCATTGCCCAACTATTTGCTCGATCGCTCGGACTTTGCAGAATGCATTGGCATGGAGGAATCCAAGGAATTCGCCTTACAATTGTTTGATGCGCTTAGTAGAAGAAGAGCGAGAGGCCGACACTTAAAGAGCATCTCCAAGGACGAATTGCACGAGTACTGxxxxxxxxxxxxxxxGAACCGGAGCTTCGAATCGAGAATACAAATTTTCTTCGACCTCTGCGATAAAGATTCAGACGGTCGTATCTCAGAAGCAGAAGTGAGGGAGGTGATTCTGCTGAGTGCATCGGCGAATAGGCTTTCAATATTGCAAGAACAGGCTGATGAAATATGCAGC

Fln Alignment:

Contig13990___RTKKPRLEKSLSVAEHAILGLRFINRATCDKDLDKLWAEVECRFNKLALPNYLLDRSDFAECIGMEESKEFALQLFDALSRRRARGRHLKSISKDELHEYWxxxxxNRSFESRIQIFFDLCDKDSDGRISEAEVREVILLSASANRLSILQEQADE
Q948U0_______________RRQRAQLDRTRSSAHKALRGLKFISNNKTNG-----WNEVENNFAKLAKDGYLY-RSDFAQCIGMKDSKEFALELFDALSRRRR--LKVDKISKEELYEYWxxxxxDQSFDSRLQIFFDMVDKNEDGRIGEEEVKEIIMLSASANKLSRLKEQAEE

SNPs (tot: 2)

Position	A %	C %	G %	T %	Probability
130	66	33			0.997452
131	66			33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain