SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene11414

Length: 1050 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene11414

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Sulfate/bicarbonate/oxalate exchanger and transporter sat-1 n=5 Tax=Populus RepID=B9H8C3_POPTR	-	-	62%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	59%
Blast2go	sulfate bicarbonate oxalate exchanger and transporter sat-1	-	-	82%

Source	GOs	Term	Type	Identity
Blast2go	sulfate transport	GO:0008272	Biological Process	82%
Blast2go	transmembrane transport	GO:0055085	Biological Process	82%
Blast2go	secondary active sulfate transmembrane transporter activity	GO:0008271	Molecular Function	82%
Blast2go	integral to membrane	GO:0016021	Cellular Component	82%

Source	InterPros	Term	Type	Identity
Sma3	Sulphate anion transporter	IPR001902	-	-
Sma3	Sulphate transporter/antisigma-factor antagonist STAS	IPR002645	-	-
Sma3	Sulphate transporter	IPR011547	-	-
Sma3	Heavy-metal-associated, conserved site	IPR017969	-	-
Sma3	Sulphate anion transporter, conserved site	IPR018045	-	-
Sma3	Ribosomal protein S2, conserved site	IPR018130	-	-

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LKN3

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 331 aas, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 316 - 680

Fln protein: MEGALNAQNDTNGGFYDNDLEMAVVHKVSLGPPKSTLNRIEGAVKETLFPDDPFRQFKNQPAKRKCLLGVQYVFPILEWAPKYSIHLLKSDIISGLTIASLAIPQGISYAKLANLPPILGLYSSFVPPLVYAMLGSSKDLAVGTVAVVSLLLTSLFSSEVSPVENPVLYLHLAFTATFLAGIFQTALGIFRLGFIIDFLSHATIIGFMAGAATVVSLQQLKGILGLHQFTKKTDIVSVMRSVWGQTHQWRWETIVMGLSFLCLLLVTRHISKKMPRLFWISAATPLTTVIVGSLMVFVTHADKHGVAIVGDLKRD*

Protein Length: 317

Fln nts: ATCTGAGTAAGTTGCTCGTGTAATACCTATGGAAGTCTAATCAGCTCAGTTTTCCGGCTGTTCAAGCAAGAAAGGAAACAATTGAAGCCC_-_ATGGAGGGAGCACTGAATGCCCAGAACGATACCAATGGTGGTTTTTATGATAATGATTTGGAGATGGCTGTGGTTCATAAAGTGAGCCTCGGGCCTCCGAAGAGTACACTGAATCGCATTGAAGGGGCTGTGAAGGAGACTCTGTTTCCGGACGACCCCTTTAGACAGTTCAAAAATCAGCCAGCAAAACGGAAGTGTTTGCTGGGAGTTCAGTATGTGTTTCCAATACTTGAATGGGCACCCAAATATTCAATACATCTTCTAAAGTCTGATATTATCTCTGGATTAACTATAGCCAGCCTGGCCATACCGCAGGGTATAAGCTATGCTAAGTTGGCCAATCTTCCACCTATTCTTGGCCTTTACTCCAGCTTCGTCCCACCTCTCGTTTATGCAATGCTAGGAAGTTCGAAGGACCTTGCCGTGGGAACCGTTGCCGTTGTATCTCTCCTTCTGACATCCCTCTTTAGCAGTGAGGTCTCTCCAGTGGAGAATCCAGTCTTATATTTGCACTTGGCCTTCACAGCAACCTTTCTTGCAGGGATTTTTCAAACAGCTTTGGGCATTTTCAGGTTGGGATTCATCATAGACTTCCTGTCACATGCAACTATAATAGGATTCATGGCGGGCGCAGCTACAGTCGTGTCCCTTCAACAACTAAAAGGCATACTTGGACTCCACCAATTTACGAAAAAGACAGACATAGTATCTGTCATGCGTTCAGTCTGGGGACAAACACACCAGTGGAGATGGGAGACCATAGTAATGGGGTTATCATTTCTCTGCCTTCTCCTCGTTACCCGCCACATTAGCAAGAAAATGCCCAGGTTATTTTGGATCTCAGCTGCTACTCCTCTGACAACTGTGATTGTGGGCAGCCTGATGGTCTTTGTAACACACGCTGACAAACATGGGGTTGCTATTGTTGGTGACCTCAAAAGGGATTGAATTCATTGTCCA

Fln Alignment:

Contig11414___VVHKVSLGPPKSTLNRIEGAVKETLFPDDPFRQFKNQPAKRKCLLGVQYVFPILEWAPKYSIHLLKSDIISGLTIASLAIPQGISYAKLANLPPILGLYSSFVPPLVYAMLGSSKDXXXXXXXXXXXXXXXXXXXXXXPVENPVLYLHLAFTATFLAGIFQTALGIFRLGFIIDFLSHATIIGFMAGAATVVSLQQLKGILGLHQFTKKTDIVSVMRSVWGQTHQWRWETIVMGLSFLCLLLVTRHISKKMPRLFWISAATPLTTVIVGSLMVFVTHADKHGVAIVGDLKR
B8LKN3_______________VVHKVGIPESKGLVHQFNSQVKETLFPDDPFREFKGKPFGRKLWLGFRYFFPILEWAPNYSLNLFKSDVISGITIASLAIPQGISYARLANLPPIHGLYCSFVPPLIYSVLGSSRDLAVGPVSIASLLLAVMLREEVSPIDAPVLYLQLALTATFFAGVFQASLGILRLGFIIDFLSRATLVGFMAGAAIIVSLQQFKGFLGIQHFTPNMDVVSVLHSVLERRDEWTWQSTLMGVFFLSFLLIARYYSQKKPKLFWISAAAPLASVILATTFIFFTRSENHSISTIGHLQK

SNPs (tot: 9)

Position	A %	C %	G %	T %	Probability
25	66		33		0.997452
76	66		33		0.997452
328	14		85		0.993533
331		85		14	0.993533
332		85		14	0.993533
375	83		16		0.994512
491	16			83	0.994512
493			83	16	0.994512
495	83			16	0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain