SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene10823

Length: 2303 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene10823
AACGTCGTGAACTTGCCTATCAGTCAGTTTTACCAGGAGAGATGTGCTCTGTGAAGTTGAGTAATGCGGAACTTGATAACTGCTTGCGTCCTGGAGATGTTATTCATAAACTTAAGCTGCAGGTGCTGGACGCTTATGAAAATCCTGTTGAGAAAGGACGAAAAATAAAGGTGGAATTGACAGGATTAGAGCTCCAAGACCAGGAGTTCCAAGACCAGGATGACCCATATCGAGAGTATCGGGAGGTGGATGATCAAGGTTGGATAAACTTTGGAGGGCTTCTAAAAGTTACGGGAAATTATGGTTCAAAAGGTTGCATTCGTGTCTCCTCAGATACAAAATTGTTGCTTAACAAAGAGTTTGACCTTCTATCAAGGGAGCTCAAAGTTGTATCCGGGATTCCAGAGCATTCTTATGCTGGTTCTATTCTAAAGAATGTTGTGTTTGGAATCTTTGATGACGAAGGGAATCTTGATTCGAAAATGGATGGTTTCCAGCATACATTGACAGTTGATTTGTGTACTCTGAGTTCTGTTCAATACAAATTCAGTCAGGGAAAGTGTACGGTGCCCAAAATTATGCTTCCAGAAGACATTAGACTGTTGCAATTTAAAGCTTTCCATTCCTGGCATACCGAGCTTTTTGTGCATATTAAGGTTGATATTCTTCCAAATCCACGGCTGGATATTGTTAGTGTTCCGGAACAAACTTTCGATGATGGAGCAATCTGTCATGCTTACGATGAGAGCACAGCAAGTCAATCACAAATAAGTAATCAAACTATGGTGAATGAAGTTAGATTGTACATTGAGACTTTTATGAGTGAGATGACGAAATGGGAGAATGATTTGAAGCAACTTGGGACCAAAGTTAAAAAGCGTGAGGATGAACTGAGGAAATTAGAAACCAATTTAAGTGAAGTGAATAGTTGTCTGAGTGACTTGGAAAGAGAGCTCAACAAACGTAACTTTTCTTCAAAAAGTGATGGGAAGGCAAATTCAAATTCATATTCACACAGTGTTGAAGATACTATAGAAGAAATCAAAAGACAGGGAATCACTGCAGCTTCCCTCTGGCTTCACTTAAGTAGGGTGGATGTTCAATCAAGAAAAACCCCTATCATACAGGACGTGGTTGGAGTTGTTGCCTTGTTAGGGAAAGTAGAGGATGATGCCATCAGTCGGGCACTATCCGAGTATGTGGGAAAGGAGAATATGCTCGCAATTGTCTGCAAGTCTCGTCAAGGGGTTGAAGCACTTGAACGATATGATGACTCTGGTAACATAATGACAGAAAGAGGTCTTCATGGGCTAGCAGGTGCCCAAAAAGCAAGAATTAAAGGAAGGTTCCGTGTGATTTGCCTTGAGGACTTAAGGTCTTATGAGGGGGGATTCAATGCTGGTGATCCACAAAAGAAACTTATTATGGAGGATCCAAAATCATCAGATGGAATCCTCCCTGAAGGATATATGGGATATGCTGTAAATATGATAAAGTTGGATCACGAGGAGCTATACCCTTCAACTTCGTCAAGGATTGAATTGAGAGGAACATTATTTTTTAATCTGTTCTCATATCTGCAAGTCTACGAAACAAGAAAGCACATGAATCAAGCCATATCTTATATAAGAGACGGGGCTATCTCATTGGATGGAGGCATGCTGAGGAGAAAAGGTGTTATTGAATGTGGAAATAGGGACGAAGTTGCTGTGAGCTTTCCGACTACTTCAGCGAAAACATCAGCGAAAACATCGCCCTTTACCTCAGCAAAAGCAATGCCGTCGCGCTTTTATCTGCGCGTTGTTATTTTGGCTGTCATTGCAAAGAAGATGGAAGAAAAAGAGATGTTGGAGAAACAAATCTGTCGTGTCCGTGAGAAACGGGAACGTCTTAAAGAAAGAATTTGATAATAAAACGAAAGAACTTGAAGAGAGAGATGTTCGAAAGAACTTGTTTATGGAAAATGTACAGCAGCAAGGCTTATTATTGGAGCACCACGAGCAGTCTGATTAAATCATAGATTTTTAGATATGATCTTCTGTCTGGAATCATATATTTTGGTCTTACTTTTGCAGTGAACAATTTACAGAAGTCCTGTGACTGATTAAATATTAAAATGTCAAGATACATCTCAAGGTCAGACAAAATTGTAAAAGCGATATCAGAAAATTTGGAGCGTTAGCTGTTGTGATTAACGTGTGTTTTTGACTTTATCGGAGATAAGAATATAGTTTTTTGTACAGTTATGTATGTTCAAACATATGAATTTTCACAATGTTTATTTATATATATTTTCTTTGACT

Ace file of the UniGene sp_v2.0_unigene10823 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	protein defective in meristem silencing 3 [Arabidopsis thaliana] gb\|AAK83630.1\| AT3g49250/F2K15_110 [Arabidopsis thaliana] gb\|AAL90947.1\| AT3g49250/F2K15_110 [Arabidopsis thaliana] gb\|AEE78515.1\| protein defective in meristem silencing 3 [Arabidopsis thal	-	-	39%
FL-Next	tr=Putative uncharacterized protein; Vitis vinifera (Grape).	-	-	34%
Blast2go	protein	-	-	55%

Source	GOs	Term	Type	Identity
Blast2go	DNA methylation	GO:0006306	Biological Process	55%
Blast2go	gene silencing by RNA	GO:0031047	Biological Process	55%
Blast2go	protein binding	GO:0005515	Molecular Function	55%

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: tr_plants

Fln subject: A5AJP7

Fln msg: query STOP codon is far from subject stop. Distance to subject end: 19 aas, your sequence is shorter than subject: 635 - 1117

Fln protein: RRELAYQSVLPGEMCSVKLSNAELDNCLRPGDVIHKLKLQVLDAYENPVEKGRKIKVELTGLELQDQEFQDQDDPYREYREVDDQGWINFGGLLKVTGNYGSKGCIRVSSDTKLLLNKEFDLLSRELKVVSGIPEHSYAGSILKNVVFGIFDDEGNLDSKMDGFQHTLTVDLCTLSSVQYKFSQGKCTVPKIMLPEDIRLLQFKAFHSWHTELFVHIKVDILPNPRLDIVSVPEQTFDDGAICHAYDESTASQSQISNQTMVNEVRLYIETFMSEMTKWENDLKQLGTKVKKREDELRKLETNLSEVNSCLSDLERELNKRNFSSKSDGKANSNSYSHSVEDTIEEIKRQGITAASLWLHLSRVDVQSRKTPIIQDVVGVVALLGKVEDDAISRALSEYVGKENMLAIVCKSRQGVEALERYDDSGNIMTERGLHGLAGAQKARIKGRFRVICLEDLRSYEGGFNAGDPQKKLIMEDPKSSDGILPEGYMGYAVNMIKLDHEELYPSTSSRIELRGTLFFNLFSYLQVYETRKHMNQAISYIRDGAISLDGGMLRRKGVIECGNRDEVAVSFPTTSAKTSAKTSPFTSAKAMPSRFYLRVVILAVIAKKMEEKEMLEKQICRVREKRERLKERI*

Protein Length: 636

Fln nts: AACGTCGTGAACTTGCCTATCAGTCAGTTTTACCAGGAGAGATGTGCTCTGTGAAGTTGAGTAATGCGGAACTTGATAACTGCTTGCGTCCTGGAGATGTTATTCATAAACTTAAGCTGCAGGTGCTGGACGCTTATGAAAATCCTGTTGAGAAAGGACGAAAAATAAAGGTGGAATTGACAGGATTAGAGCTCCAAGACCAGGAGTTCCAAGACCAGGATGACCCATATCGAGAGTATCGGGAGGTGGATGATCAAGGTTGGATAAACTTTGGAGGGCTTCTAAAAGTTACGGGAAATTATGGTTCAAAAGGTTGCATTCGTGTCTCCTCAGATACAAAATTGTTGCTTAACAAAGAGTTTGACCTTCTATCAAGGGAGCTCAAAGTTGTATCCGGGATTCCAGAGCATTCTTATGCTGGTTCTATTCTAAAGAATGTTGTGTTTGGAATCTTTGATGACGAAGGGAATCTTGATTCGAAAATGGATGGTTTCCAGCATACATTGACAGTTGATTTGTGTACTCTGAGTTCTGTTCAATACAAATTCAGTCAGGGAAAGTGTACGGTGCCCAAAATTATGCTTCCAGAAGACATTAGACTGTTGCAATTTAAAGCTTTCCATTCCTGGCATACCGAGCTTTTTGTGCATATTAAGGTTGATATTCTTCCAAATCCACGGCTGGATATTGTTAGTGTTCCGGAACAAACTTTCGATGATGGAGCAATCTGTCATGCTTACGATGAGAGCACAGCAAGTCAATCACAAATAAGTAATCAAACTATGGTGAATGAAGTTAGATTGTACATTGAGACTTTTATGAGTGAGATGACGAAATGGGAGAATGATTTGAAGCAACTTGGGACCAAAGTTAAAAAGCGTGAGGATGAACTGAGGAAATTAGAAACCAATTTAAGTGAAGTGAATAGTTGTCTGAGTGACTTGGAAAGAGAGCTCAACAAACGTAACTTTTCTTCAAAAAGTGATGGGAAGGCAAATTCAAATTCATATTCACACAGTGTTGAAGATACTATAGAAGAAATCAAAAGACAGGGAATCACTGCAGCTTCCCTCTGGCTTCACTTAAGTAGGGTGGATGTTCAATCAAGAAAAACCCCTATCATACAGGACGTGGTTGGAGTTGTTGCCTTGTTAGGGAAAGTAGAGGATGATGCCATCAGTCGGGCACTATCCGAGTATGTGGGAAAGGAGAATATGCTCGCAATTGTCTGCAAGTCTCGTCAAGGGGTTGAAGCACTTGAACGATATGATGACTCTGGTAACATAATGACAGAAAGAGGTCTTCATGGGCTAGCAGGTGCCCAAAAAGCAAGAATTAAAGGAAGGTTCCGTGTGATTTGCCTTGAGGACTTAAGGTCTTATGAGGGGGGATTCAATGCTGGTGATCCACAAAAGAAACTTATTATGGAGGATCCAAAATCATCAGATGGAATCCTCCCTGAAGGATATATGGGATATGCTGTAAATATGATAAAGTTGGATCACGAGGAGCTATACCCTTCAACTTCGTCAAGGATTGAATTGAGAGGAACATTATTTTTTAATCTGTTCTCATATCTGCAAGTCTACGAAACAAGAAAGCACATGAATCAAGCCATATCTTATATAAGAGACGGGGCTATCTCATTGGATGGAGGCATGCTGAGGAGAAAAGGTGTTATTGAATGTGGAAATAGGGACGAAGTTGCTGTGAGCTTTCCGACTACTTCAGCGAAAACATCAGCGAAAACATCGCCCTTTACCTCAGCAAAAGCAATGCCGTCGCGCTTTTATCTGCGCGTTGTTATTTTGGCTGTCATTGCAAAGAAGATGGAAGAAAAAGAGATGTTGGAGAAACAAATCTGTCGTGTCCGTGAGAAACGGGAACGTCTTAAAGAAAGAATTTGA___TAATAAAACGAAAGAACTTGAAGAGAGAGATGTTCGAAAGAACTTGTTTATGGAAAATGTACAGCAGCAAGGCTTATTATTGGAGCACCACGAGCAGTCTGATTAAATCATAGATTTTTAGATATGATCTTCTGTCTGGAATCATATATTTTGGTCTTACTTTTGCAGTGAACAATTTACAGAAGTCCTGTGACTGATTAAATATTAAAATGTCAAGATACATCTCAAGGTCAGACAAAATTGTAAAAGCGATATCAGAAAATTTGGAGCGTTAGCTGTTGTGATTAACGTGTGTTTTTGACTTTATCGGAGATAAGAATATAGTTTTTTGTACAGTTATGTATGTTCAAACATATGAATTTTCACAATGTTTATTTATATATATTTTCTTTGACT

Fln Alignment:

Contig10823___DNCLRPGDVIHKLKLQVLDAYENPVEKGRKIKVELTGXXXXXXXXXXXXDPYREYREVDDQGWINFGGLLKVTGNYGSKGCIRVSSDTKLLLNKEFDLLSRELKVVSGIPEHSYAGSILKNVVFGIFDDEGNLDSKMD-----GFQHTLTVDLCTL---SSVQYKFSQGKCTVPKIMLPEDIRLLQFKAFHSWHTELFVHIKVDILPNPRLDIVSVPEQTFDDGAICHAYDESTASQSQISNQTMVNEVRLYIETFMSEMTKWENDLKQLGTKVKKREDELRKLETNLSEVNSCLSDLERELNKRNFSSKSDGKANSNSYSHSVEDTIEEIKRQGITAASLWLHLSR-VDVQSRKTPIIQDVVGVVALLGKVEDDAISRALSEYVGKENMLAIVCKSRQGVEALERYDDSGNIMTERGLHGLAGAQKARIKGRFRVICLEDLRSYEGGFNAGDPQKKLIMEDPKSSDGILPEGYMGYAVNMIKLDHEELYPSTSSRIELRGTLFFNLFSYLQVYETRKHMNQAISYIRDGAISLDGGMLRRKGVIECGNRD
A5AJP7_______________DNQLLPGCVIEELVLEMFDAYGNHAREGLEVQFNVDGFCFQDHNGLK--------RKVDDRGCIDLSGLLRVTTGYGKNVSLSVLSGNKVVFKQELQTEKRELRAASIVPQSCAAGSQLENIVFEIINSKGEVDETVHEEEKHGQFHTLTIMSDSFYLDGSVRFAFRNGRCIIPTIPLPRKQGDFTFLAAHSCHPELSLAVKVSVV-----EVLKVKQE---DVQLQYPNENMLLLQDSPAPRHVENSL---VESLMNDEKEIEDDICKIGLFIGDNE---RKLEL----LHKQKGDIEQSIEKLQASVEYDSFNNHRGYLSKKESVMRCIEKKDKSAAAFFCNLSREIPFQDPVSQLMKDIVGVVALLATVRINRLGRMLAEYLGEDQMLAVVCRSYEAASKLEKYEWDGKVDREHALYAVAKTFGKPINDRFLVICLENIRPYIGGFQDNDPQRKLNIPNPILPTGEMPPGFLGYAVNMVDLESHHLLTRTTAGHGLRETLFYCLFGELQVYQTREDMKKACFYARHGAVSLDGGIMKGNGVISFGCRE

SNPs (tot: 72)

Position	A %	C %	G %	T %	Probability
185		60		40	1.0
395			20	80	0.995493
398		80	20		0.995493
400	80	20			0.995493
402			20	80	0.995493
403		20		80	0.995493
405	20			80	0.995493
406	20			80	0.995493
407			20	80	0.995493
408		80	20		0.995493
410		20	20	60	0.99255
411	20			60	0.99255
412	80		20		0.995493
413	60	20		20	0.99255
417	20		80		0.995493
418			25	75	0.996473
419		25		75	0.996473
421	25	75			0.996473
422	25	75			0.996473
424			25	75	0.996473
428	25			75	0.996473
441		66	33		0.997452
442	66			33	0.997452
445		33		66	0.997452
446	33			66	0.997452
447			33	66	0.997452
448		33	66		0.997452
449		33		66	0.997452
450	33			66	0.997452
451	33			66	0.997452
452		66	33		0.997452
458	66		33		0.997452
461	66		33		0.997452
878	33			66	0.997452
879	33	66			0.997452
880		66		33	0.997452
881	66		33		0.997452
1140		85		14	0.993533
1141		85	14		0.993533
1142	14			85	0.993533
1143		85	14		0.993533
1147	16			83	0.994512
1148			16	83	0.994512
1149		16		83	0.994512
1151	16	83			0.994512
1152	16	83			0.994512
1153			16	83	0.994512
1154	83		16		0.994512
1155	83			16	0.994512
1158	83	16			0.994512
1160	16		83		0.994512
1162	83		16		0.994512
1477	14	85			0.993533
1480		85	14		0.993533
1482	14			14	0.984337
1492	85	14			0.993533
1494			85	14	0.993533
1507	83			16	0.994512
1510		20		80	0.995493
1511	80	20			0.995493
1883	80	20			0.995493
1884	80		20		0.995493
1885	20			80	0.995493
1889	80		20		0.995493
1969	75			25	0.996473
1985		50	50		1.0
2025		66		33	0.997452
2178	85	14			0.993533
2290		60	40		1.0
2294		50		50	1.0
2297		50	50		1.0
2302			50	50	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain