SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene9922

Length: 1200 nt

Origin of reads: Biogeco1 v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene9922

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	topoisomerase 6 subunit B-like protein [Oryza sativa Japonica Group]	-	-	68%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	90%
Blast2go	dna topoisomerase 6 subunit b	-	-	82%

Source	ECs	Term	Type	e value	Identity
Blast2go	DNA topoisomerase (ATP-hydrolyzing).	EC:5.99.1.3	-	0.0	82%

Source	GOs	Term	Type	Identity
Blast2go	trichome differentiation	GO:0010026	Biological Process	82%
Blast2go	response to brassinosteroid stimulus	GO:0009741	Biological Process	82%
Blast2go	DNA endoreduplication	GO:0042023	Biological Process	82%
Blast2go	DNA topological change	GO:0006265	Biological Process	82%
Blast2go	pattern specification process	GO:0007389	Biological Process	82%
Blast2go	identical protein binding	GO:0042802	Molecular Function	82%
Blast2go	ATP binding	GO:0005524	Molecular Function	82%
Blast2go	DNA topoisomerase (ATP-hydrolyzing) activity	GO:0003918	Molecular Function	82%
Blast2go	DNA topoisomerase complex (ATP-hydrolyzing)	GO:0009330	Cellular Component	82%
Blast2go	chromosome	GO:0005694	Cellular Component	82%

Source	InterPros	Term	Type	Identity
Sma3	ATPase-like, ATP-binding domain	IPR003594	-	-
Sma3	Ribosomal protein S5 domain 2-type fold, subgroup	IPR014721	-	-
Sma3	DNA topoisomerase VI, subunit B, transducer	IPR015320	-	-

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: D5AEH0

Fln msg: STOP codon was not found. Distance to subject end: 7 aas, your sequence is shorter than subject: 400 - 472

Fln protein: FTWGDNNDRWNGAEIQVTIEGNWTTYRSKIIQYMRQMAVITPYAQFLFRYISATADKNVTIKFARRTDVMPPVPIETNYHPSAVDLLLIKRLIAETSKQNLIQFLQHEFVNISKSYADRLIGEMGPDFTPKMAVKTLSSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYEGSPHCFEGHPFIVEAGVSLGGKDVKQGINVFRFANRIPLLFEQGADVVTRTAMKRINTKIPFKGTGKEYIGDDITEIVTAVKAAIQQCCVQLKSKIVRKQQARERQERKRNLTKYIPDASRAIYEVLKDIVQLRTPKKPRYGDVYEGILNGVSSREITETTLRDKLAQHVEQVDIEMALEYATQSGITLEARETTYIRSLEGTQNFYDFHSPV

Protein Length: 401

Fln nts: TTTACATGGGGGGACAACAATGATAGATGGAATGGGGCAGAAATTCAAGTTACAATTGAAGGAAATTGGACAACTTATCGTTCAAAGATAATACAGTATATGCGCCAAATGGCTGTAATAACACCATATGCTCAGTTTCTCTTCCGATACATTTCAGCAACAGCGGACAAGAATGTCACAATAAAATTTGCACGAAGGACAGATGTGATGCCTCCAGTACCTATTGAAACGAACTATCATCCTTCTGCTGTAGATCTATTACTTATCAAGCGCCTTATTGCAGAGACATCGAAGCAGAACCTTATCCAGTTTCTCCAACATGAGTTTGTTAATATTAGCAAATCTTATGCTGATCGCTTAATTGGTGAGATGGGTCCAGATTTTACGCCCAAGATGGCTGTGAAAACTCTTTCATCTCAACAGATAGTTCGAATCCATCAACTCTTCCGGCAAGCTAAGTTTGATGATCCTAGTGGTGATTGTCTTAGTCCTGCAGGGGAATACAATCTTCGTCTTGGGATCATAAAAGAGCTACATCCAGACATGGTTGCAACATACGAGGGAAGTCCACATTGTTTTGAAGGTCATCCGTTTATCGTGGAGGCAGGGGTCAGTCTAGGTGGAAAAGATGTTAAGCAGGGCATCAATGTATTTAGATTTGCAAACCGCATTCCACTTTTGTTTGAACAAGGGGCAGATGTCGTCACAAGGACTGCTATGAAGCGGATCAATACAAAGATTCCCTTCAAGGGAACCGGCAAAGAATATATTGGAGATGACATAACAGAAATAGTTACTGCTGTCAAGGCTGCTATCCAACAATGTTGTGTTCAGTTGAAGTCAAAAATCGTTAGAAAACAACAAGCACGCGAGCGACAAGAGCGTAAACGAAACCTGACCAAGTACATACCTGATGCTTCACGTGCTATATATGAGGTGCTAAAAGATATCGTACAACTTAGGACACCGAAAAAGCCAAGATATGGGGATGTATATGAAGGAATACTCAACGGAGTATCATCCCGTGAGATTACAGAAACAACCTTGAGAGACAAGCTCGCCCAGCATGTTGAACAGGTTGATATTGAAATGGCACTTGAATATGCTACACAAAGTGGAATAACCCTGGAAGCTCGTGAGACTACGTATATACGTTCTTTGGAGGGAACTCAAAACTTTTATGACTTCCATAGTCCTGTT

Fln Alignment:

Contig9922___DNNDRWNGAEIQVTIEGNWTTYRSKIIQYMRQMAVITPYAQFLFRYISATADKNVTIKFARRTDVMPPVPIETNYHPSAVDLLLIKRLIAETSKQNLIQFLQHEFVNISKSYADRLIGEMGPDFTPKMAVKTLSSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYEGSPHCFEGHPFIVEAGVSLGGKDVKQGINVFRFANRIPLLFEQGADVVTRTAMKRIN---------------------TKIPFKGTGKEYIGDDITEIVTAVKAAIQQCCVQLKSKIVXXXXXXXXXXXXXNLTKYIPDASRAIYEVLKDIVQLRTPKKPRYGDVYEGILNGVSSREITETTLRDKLAQHVEQVDIEMALEYATQSGITLEARETTYIRSLEGTQNFYDFHSPV
D5AEH0______________DNNDKWNGAEIQVTIEGNWTTYRSKIIQYMRQMAVITPYAQFLFRYVSATVDKNVTIRFARRTDVMPPVPIETNYHPSAVDLLLIKRLIAETSKQNLIQFLQHEFVNISKSHADRLIGEMGPDFTPKMAVKSLSSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYEGSPHVFEGHPFIVEAGISLGGKDVKQGINVFRFANRIPLLFEQGADVITRTAMKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDITEIATSVKAAIQQCCVQLKSKIVRKQQARERQERKRNLTKYIPDASRAIYEVLKDIVQLRSPKKPRYGDVYEEILDRVSSREITETTLREKLAQHVEQVDIEMALEYATQSGITQEARETTYIRALEGVQNFYDFHSPV

SNPs (tot: 3)

Position	A %	G %	T %	Probability
186	33	66		0.997452
187	66	33		0.997452
188	33		33	0.997753

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain