SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene9705

Length: 1451 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco3 v1 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene9705 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	oxidoreductase, 2OG-Fe(II) oxygenase family protein [Arabidopsis thaliana] gb\|AAD03425.1\| contains similarity to Iron/Ascorbate family of oxidoreductases (Pfam: PF00671, Score=297.8, E=1.3e-85, N=1) [Arabidopsis thaliana] emb\|CAB40043.1\| putative Fe(II)/a	-	-	49%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	88%
Blast2go	2og-fe oxygenase family protein	-	-	73%

Source	ECs	Term	Type	e value	Identity
Blast2go	Oxidoreductases, Acting on paired donors, with incorporation or reduction of molecular oxygen, With 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors.	EC:1.14.11.-	-	0.0	73%

Source	KEGGs	Term	Identity
Blast2go	Glycine, serine and threonine metabolism	00260	73%
Blast2go	Benzoxazinoid biosynthesis	00402	73%
Blast2go	Diterpenoid biosynthesis	00904	73%

Source	GOs	Term	Type	Identity
Blast2go	secondary metabolic process	GO:0019748	Biological Process	73%
Blast2go	oxidation-reduction process	GO:0055114	Biological Process	73%
Blast2go	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors	GO:0016706	Molecular Function	73%

Source	InterPros	Term	Type	e value	Identity
Sma3	Aminoacyl-tRNA synthetase, class I, conserved site	IPR001412	-	0.0	-
Sma3	Oxoglutarate/iron-dependent oxygenase	IPR005123	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9P1C4

Fln msg:

Fln protein: MPSTMEDVLTQDVNAVEKLLSNGINHLQVQDKYILPPSERPHLSEVSYSESIPVVDLQDLDGPNRTRVVEEIRHACEEDGFFQIVNHGVPEGTMKSMMEIAKEFFEMPVEDRACLYSENPNQRVRLATSFNIRKEKVLNWRDYLLHPCHPLEEVISSWPEKPAAYREIAGNYAMKVRALTLRLLAAVSEALGLDSSYLDRIFGKHTQLMDIMYYPPCPKPDITLGTPRHSDARGITVLMQGEISGLQVLRNGKWVAVEPIPNAFVVNMGDQLQVVSNGRFRSVEHRAVTNASTARISIPTFYGPSEDAFIAPAASLVDEQHPALYRGYKFGEFLRFFWSQELKSKTVLDHFKIEYPENNGE*

Protein Length: 363

Fln nts: ATGGGGTCAATTCATGGGCAGAGATCTGCACGCACGCACTTAAATGTTCACTCTTCTTTTGCCCATCATCCGATATCCTATTTCTGGTCTGCGAATCGAAGCTTAGTTTTGCACAGCAATAGGTAGGTGCCACTGCCAATTTTACAGCACTCGAGAAAGAAAT_-_ATGCCGTCTACAATGGAAGATGTGCTGACCCAGGATGTGAATGCTGTCGAAAAGCTGCTGTCCAATGGAATAAATCATCTTCAAGTTCAAGATAAATATATCCTGCCTCCCAGTGAACGTCCCCACCTGTCTGAGGTGTCTTACTCTGAGAGCATTCCGGTGGTGGATCTCCAGGACCTGGATGGCCCTAACAGAACAAGGGTAGTTGAAGAAATCCGCCATGCGTGCGAGGAAGATGGCTTTTTCCAGATAGTGAACCATGGAGTACCCGAAGGAACCATGAAAAGCATGATGGAAATTGCCAAAGAATTCTTCGAAATGCCCGTGGAAGACCGAGCATGCCTCTACTCAGAGAACCCTAATCAGCGGGTTAGGCTTGCTACGAGCTTCAACATTCGCAAAGAAAAAGTCCTTAACTGGAGGGATTATCTGTTGCACCCATGTCATCCTTTGGAGGAAGTCATCAGCTCTTGGCCTGAAAAACCTGCAGCCTACAGAGAGATCGCAGGCAACTACGCTATGAAGGTGAGAGCTCTGACTTTAAGGCTGCTGGCTGCCGTTTCTGAAGCTCTAGGGCTCGATTCCAGTTACTTGGACAGAATCTTCGGAAAACACACCCAACTCATGGATATAATGTACTATCCTCCATGCCCGAAACCGGATATTACTCTTGGCACACCACGCCATTCAGATGCGCGTGGCATCACTGTTTTAATGCAAGGCGAGATCAGTGGTCTGCAAGTACTCAGAAATGGAAAGTGGGTTGCTGTAGAACCCATACCGAACGCTTTCGTTGTTAATATGGGAGATCAACTGCAGGTTGTGAGCAATGGTAGATTCAGAAGTGTGGAACATCGAGCAGTAACGAATGCATCTACTGCACGTATATCAATTCCTACGTTTTATGGCCCTTCGGAGGATGCCTTCATTGCTCCTGCTGCGTCTCTGGTTGATGAACAGCATCCGGCTCTGTACAGAGGATATAAGTTTGGAGAATTCTTGAGATTTTTCTGGAGCCAGGAATTAAAGAGTAAGACCGTGTTGGATCATTTCAAGATCGAATATCCAGAGAATAATGGTGAATGA___ATTTTGTTACGGGGATTGATCCAGGGGAGGCATCATTATCCTTCGTGTCAATTGTACCCTTTTCCCTGGAATGGCCCTACTGTGCTGCCCAGCACTTTGCCTCTGGACAAATATTCGGGAGCGAAGAAATTTCATTAACATCATCTACCAAGAATGAGTTGAGAATCTCCCTGGAGAATACATGTGCCACATTGTAAATGTT

Fln Alignment:

Contig9705___MPSTMEDVLTQDV--NAVEKLLSNGINHLQVQDKYILPPSERPHLSEVSYSESIPVVDLQDLDGPNRTRVVEEIRHACEEDGFFQIVNHGVPEGTMKSMMEIAKEFFEMPVEDRACLYSENPNQRVRLATSFNIRKEKVLNWRDYLLHPCHPLEEVISSWPEKPAAYREIAGNYAMKVXXXXXXXXXAVSEALGLDSSYLDRIFGKHTQLMDIMYYPPCPKPDITLGTPRHSDARGITVLMQGEISGLQVLRNGKWVAVEPIPNAFVVNMGDQLQVVSNGRFRSVEHRAVTNASTARISIPTFYGPSEDAFIAPAASLVDEQHPALYRGYKFGEFLRFFWSQELKSKTVLDHFKIEYPENNGE
A9P1C4______________MPSTMEDVLTHEAKANGVEKLMSNGINHLQVQDTYILPPSERPRLSEVSYSESIPVVDLQDLDGPNRTRVVQEICLACEEDGFFQILNHGVPEATMKSMMGIAKEFFEMPVEDRACLYSEDTNQRVRLSTSFNISKEKVLNWRDYLLHPCHPLEEFIGSWPEKPAAYREIAGNYAAEMRALILRLMAAVSEALGLDSDYLNRIFGKHSQLMDIMYYPPCPNPDITLGTPRHSDARGITVLMQGNVSGLQVLRNGKWVAVEPIPNAFVVNMGDQLQVVSNGRFRSVEHRAVTNTSTARISIPTFYGPSEEAFIAPAESLVDEQHPALYKGFKFGEFMKIFWGQELKNKTVLDHFKIEYPENNGK

SNPs (tot: 20)

Position	A %	C %	G %	T %	Probability
499	85		14		0.993533
568	10	90			0.90298
569	10			90	0.90298
740		66	33		1.0
750	11		88		0.923564
814		12	87		0.992554
896	14		85		0.993533
1169	68		15		1.0
1231	18		81		1.0
1340		16	83		0.999991
1387		10		90	0.90298
1388	10		90		0.90298
1389			90	10	0.90298
1390			90	10	0.90298
1408		11		88	0.923564
1420		71		28	1.0
1421		14	85		0.993533
1422		14		85	0.993533
1423		14	85		0.993533
1426			85	14	0.993533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain