SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene7173

Length: 1618 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene7173

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	DUF868 domain containing protein	-	-	52%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	60%
Blast2go	protein	-	-	71%

Source	InterPros	Term	Type	Identity
Sma3	Protein phosphatase 2C, manganese/magnesium aspartate binding site	IPR000222	-	-
Sma3	Ribosomal protein L24e-related	IPR000988	-	-
Sma3	Phosphoglycerate/bisphosphoglycerate mutase, active site	IPR001345	-	-
Sma3	Protein phosphatase 2C-like	IPR001932	-	-
Sma3	Protein of unknown function DUF868, plant	IPR008586	-	-
Sma3	TRASH	IPR011017	-	-
Sma3	Histidine phosphatase superfamily, clade-1	IPR013078	-	-
Sma3	Beta tubulin, autoregulation binding site	IPR013838	-	-
Sma3		IPR014045	-	-
Sma3	Protein phosphatase 2C	IPR015655	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LNS3

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 74 aas, putative chimeric sequence, your sequence is shorter than subject: 197 - 270

Fln protein: MTQNLITCLYQTTLAEICKFVTITWCKNLMGHGLSVHVNDSRCHYMCKVEMKPWSFWKKHGSKSFDVKGKKMDVFWDVSNAKYVCGPEPQEGFYVALICEEEIVLLLGDMQEEAYKKTHARMAFTEPTLLSRREHVFGKRYYTTKAQFGESGRTHDIVIECHTGGSKEPRLYVRVDKQVVLQVKQPDVEVQQQSDN*

Protein Length: 198

Fln nts: CGTCGTGACCTTGGCTGTCACTCATTTGTTGTGTAGCACCATACAAGCGACTGGATCTGAAAAATGTTTGAAGCATTACCTTCTTGATTTGGACAGTAATCCGGAGGTGCCCACTTGACGGACAAGTCTTCTTCTTCTGGCAGCAGA_-_ATGACACAGAACTTGATAACTTGCCTCTATCAGACCACACTAGCAGAAATCTGCAAATTTGTGACCATAACATGGTGCAAGAATCTAATGGGCCATGGTCTCAGTGTCCATGTCAATGATTCAAGGTGCCATTATATGTGCAAGGTTGAGATGAAGCCATGGTCCTTCTGGAAGAAGCATGGATCCAAGAGCTTTGATGTCAAAGGGAAGAAGATGGATGTGTTCTGGGATGTGAGCAATGCTAAATACGTATGTGGGCCTGAACCACAGGAGGGCTTCTACGTGGCCCTGATATGTGAGGAGGAGATAGTACTGCTATTGGGAGATATGCAGGAGGAGGCCTACAAGAAAACCCATGCAAGGATGGCATTCACTGAGCCCACATTGTTGTCTAGAAGAGAGCATGTGTTTGGGAAGAGATATTACACAACAAAGGCACAGTTTGGTGAAAGTGGCAGGACTCATGACATTGTGATTGAATGCCATACAGGGGGATCCAAGGAGCCAAGATTGTATGTGAGAGTTGACAAGCAGGTGGTGTTACAGGTCAAGCAACCTGATGTGGAAGTTCAACAGCAATCAGACAATTGA___GGTTGATGGAGCCTCAATACAAATCTTCTGGGATGTGCACAATTGGCTCTTCAATCCCACCAACGGCCATGCTGTCTTCATGTTCCAGACTTCAGGCGATCGCATATCAAACAGTGCTAGAAGAACTGATAATGCTCCATCTGTATTCAATATCAGAGGCCATGATAAGACCTTCTTCAAGACCAGCTCCTCTTCCTCTTCCTCTTCAAATTCAAGCTCTTCAGTATTGCAATGGGCAAGCATGGAGACTGAAACTGGTAGCCCATCTGGCTTCTCATTGTTGCTGTATGCATGGAAGAGTGAATAGGGAACTCATTAGTATTACTATTTCAGTCCAAGTACTAGTAGTAGCATTACCAGTATTTCTTTCTAGTTATTGAGTCATGTACATATAAGGGAGAGAATAGCACTGGCTAGGCTGTATTCTGTGTTTTTATTTCAATTCTGACATGTATATGATTGATTAATCGAGGCCTCATTATATGGGAGCTTCTGAGACACGAATAAATCCATTCTCCAGATTTCTTTCAGGACTAGTACTGGTGCTCCTGCCACTGCTTAAGCTAATGCCAGCTAAAGGCCAATAAATGACTTCATCTGATTAAATGCCCTATTTTACAAATGAAACACACCCTCTATTGAAGTTGGGTACAAAAGTGGAGTTAGCCTCTTCATGCTTTTTGAGCTGGCCAAGCCCAGAGCTGAAACCTCTATCTCCATGTTTATCAGGCACCTACATTCTTACCATCGCCTGACAGCAAGTGGCCCATACAGACCTAATGCAAGTCCTGAGAGGTCCACACAGACCTAATGCAGCAAACATGGCATAGTTTTCCACAAGAACCAAACCCACTCTGTTCATGGTGGCGAGCAATATTTA

Fln Alignment:

Contig7173___MTQNLITCLYQTTLAEICKFVTITWCKNLMGHGLSVHVNDSRCHYMCKVEMKPWSFWKKHGSKSFDVKGKKMDVFWDVSNAKYVCGPEPQEGFYVALICEEEIVLLLGDMQEEAYKKTHARMAFTEPTLLSRREHVFGKRYYTTKAQFGESGRTHDIVIECHTGGSKEPRLYVRVDKQVVLQVK
B8LNS3______________MMQNMVTCLYLTTLSGQTRLITVTWCKNLMGQGLSVNVDDPSYQCLCRVDMKSWLFWKKKGSKRFELGTTKVDVFWDLSSARYGSAPEPLEGYFVAVVSNEEVALLLGDMCKEAFKKTHAKPP-AETVLISRKEHVFGKNCYTTKAQFCNSGRIHDIVIECQTGGPREPKLCVRIDKQLVVQVK

SNPs (tot: 32)

Position	A %	C %	G %	T %	Probability
148	87			12	0.992554
327		85		14	0.993533
609		75		25	0.996473
631		75		25	0.996473
634		33		66	0.997452
637	33		33	33	0.997753
638	33		66		0.997452
688	28		71		1.0
691	28		71		1.0
697		71	28		1.0
703		71		28	1.0
720	16		83		0.994512
721		16	83		0.994512
722	16		83		0.994512
723			83	16	0.994512
724	33	50		16	1.0
743			40	60	1.0
750	40		60		1.0
755	60		40		1.0
762	60		40		1.0
763		16		83	0.994512
764	16	83			0.994512
799		50	50		1.0
801	50	50			1.0
803		50		50	1.0
1103			9	90	0.999842
1499	50	50			1.0
1502			10	90	0.90298
1503			90	10	0.90298
1504			90	10	0.90298
1506		90	10		0.90298
1521	12		87		0.992554

SSRs (tot: 2)

Start position	End position	Sequence	Length
127	138	TCT TCT TCT TCT	12
926	945	TCCTCT TCCTCT TCCTCT TC	20

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain