SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene6480

Length: 1226 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene6480 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Eukaryotic translation initiation factor 3 subunit B n=1 Tax=Nicotiana tabacum RepID=EIF3B_TOBAC	-	-	5.0e-40	67%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	93%
Blast2go	eukaryotic translation initiation factor 3 subunit b	-	-	0.0	83%

Source	GOs	Term	Type	Identity
Blast2go	translational initiation	GO:0006413	Biological Process	83%
Blast2go	DNA binding	GO:0003677	Molecular Function	83%
Blast2go	translation initiation factor activity	GO:0003743	Molecular Function	83%
Blast2go	nucleotide binding	GO:0000166	Molecular Function	83%
Blast2go	eukaryotic translation initiation factor 3 complex	GO:0005852	Cellular Component	83%
Blast2go	nucleus	GO:0005634	Cellular Component	83%
Blast2go	cytosol	GO:0005829	Cellular Component	83%

Source	InterPros	Term	Type	Identity
Sma3	RNA recognition motif domain	IPR000504	-	-
Sma3	Six-bladed beta-propeller, TolB-like	IPR011042	-	-
Sma3	Translation initiation factor eIF-3b	IPR011400	-	-
Sma3	Nucleotide-binding, alpha-beta plait	IPR012677	-	-
Sma3	Translation initiation factor 2A, beta propellor-like domain	IPR013979	-	-
Sma3	WD40/YVTN repeat-like-containing domain	IPR015943	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9NWV8

Fln msg: Warning!, your query overlaps and the subject is separated, your sequence is shorter than subject: 290 - 724. Was predicted as Complete, but is very much shorter than de subject

Fln protein: METMEMPVPAHRLGTDTQQLNIDFSNVDLDSIQLPPGEDFGIKSEDEEEEEDSLEFETGFGNIVVVDNLPVVPPEKFEKLEGVVRKIFGQIGTIRERGLWMPLHPETQKTKGYCFIEYNTPQEAQTAKDQxxxxxxxxYAATSVTSVHEMENGFNIWSFNGKLLYHVSRDHFYQLLWRPRPPSLLSPEKEEEIAKNLRKYSKKYEAEDQDVSLLLSEQDREKRKKLQEDWQVWVNEWKRFHEEEKLFRQELRDGEPSDEEEEYEAEEVEVEEIVDVTEEIASFGFEQLD*

Protein Length: 291

Fln nts: CGTCGTGACCTTGGCTGTCACTGTATTCTCAGAGCTTTCATATCTTTACTGAAAGTCCTTCGCTGCCATAGAGAATCGCATATCTAACTCAGTTATCCACGCGTTTCCTTCTTCGGAGAGGCCTCGATATCGAGTCCTGCAGCAGCAGTAGAAGGGTTTACAAAAAAAG_-_ATGGAGACGATGGAGATGCCAGTGCCTGCCCATCGCCTGGGCACCGATACCCAGCAGCTCAACATCGATTTTTCCAATGTTGACTTGGATTCAATACAGCTTCCTCCCGGCGAAGATTTTGGCATCAAAAGTGAGGATGAAGAGGAAGAAGAAGATTCACTAGAGTTCGAGACTGGGTTCGGTAACATTGTAGTTGTAGACAATCTCCCGGTAGTCCCTCCAGAGAAGTTTGAGAAACTCGAAGGTGTTGTTCGCAAGATATTTGGGCAGATTGGTACAATCAGGGAGAGGGGGCTCTGGATGCCACTCCACCCCGAGACCCAGAAGACTAAAGGATATTGTTTTATAGAGTACAACACCCCTCAGGAAGCACAGACAGCAAAAGATCAAxxxxxxxxxxxxxxxxxxxxxxxxTATGCTGCAACATCAGTTACATCTGTGCATGAAATGGAAAATGGGTTTAACATATGGTCTTTCAATGGAAAGCTATTGTACCACGTTTCAAGAGATCATTTCTACCAGCTATTATGGCGACCGAGGCCACCCTCTCTTCTAAGCCCAGAGAAAGAGGAGGAGATCGCTAAGAATTTGAGGAAGTATAGTAAGAAGTATGAGGCTGAAGACCAGGATGTTTCGTTATTGTTGAGTGAGCAAGACAGGGAGAAACGGAAGAAATTACAAGAGGATTGGCAGGTCTGGGTCAATGAATGGAAACGTTTTCACGAGGAAGAGAAATTATTTAGACAGGAGCTACGTGATGGAGAGCCTAGTGATGAAGAAGAGGAATATGAAGCAGAAGAAGTTGAAGTTGAAGAGATCGTAGACGTTACTGAAGAGATTGCTTCATTTGGTTTTGAGCAGTTGGACTGA___TTGATTATATAGTATGCAGCTGCTTGTGCCTCCATACAGCCCTATGTTTGTGCCATTATGCAGACAGATAGCTTAGCTAGAAGATTCAGTGGTTTTTAATTGTTTTCCTGACCAGATTCATAATGGATGAGATACTCTTTTGTTGAAATTTTGTAGAATGGGCATTGAAATAGTCACTTTCTCAAAGG

Fln Alignment:

Contig6480___METMEMP--VPAHRLGTDTQQLNIDFSNVDLDSIQLPPGEDFGIKXXXXXXXXXXXXXETGFGNIVVVDNLPVVPPEKFEKLEGVVRKIFGQIGTIRERGLWMPLHPETQKTKGYCFIEYNTPQEAQTAKDQTNGYKLExxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxKWI*AGRYAATSVTSVHEMENGFNIWSFNGKLLYHVSRDHFYQXXXXXXXXXXXXXXXXXEIAKNLRKYSKKYEAEDQDVSLLLSEQDREKRKKLQEDWQVWVNEWKRFHEEEKLFRQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEIASFGFEQLD
A9NWV8______________METIEMPLPVPVHRLGNDIQQLNIDFSNVDLDSIQLPPGEDFGIKSEDEEEEEDSLEFETGFGNIVVVDNLPVVPPEKFEKLEGVVRKIFGQIGTIRERGLWMPIHPETQKTQGYCFIEYNTPQEAQTAKDQTNGYKLDxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxEWDPTGRYAATSVTVVHEMENGFNIWSFNGKLLYRIPRDHFYQLLWRPRPPSLLSPEKEEEISKNLRKYSKKYEAEDQDVSLLLSEQDREKRKRLQEDWQGWVNEWKRLHEEEKLFRQDLRDGEPSDEEEEYEAEEVEVEELIDVTEEIASFGFEQLD

SNPs (tot: 9)

Position	A %	C %	G %	T %	Probability
1062		33		66	0.997452
1063		33		66	0.997452
1135	60		40		1.0
1207	42			57	1.0
1208		42	57		1.0
1210			66	33	1.0
1212	25		75		0.996473
1214	25	75			0.996473
1216	25		75		0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain