SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene5868

Length: 2192 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

UniGene Fasta

>sp_v2.0_unigene5868
CGTCGTGACCTTGGCTGTCACTCAGGGGGTTTGTAACAGACGCAAGCCAAGCATCAGTACTCTCCAGTTTGAATTGTGCCGACTGTTGGCACAAGAGATATGATTTGTTTCTGTGATCAGGTCGAATGGCAAGCTTAGGGAAATGGCGTTTTATCAGATTTTTACCTTTCATCCTCGTACTGCCCTTTATATTTTCAGTTTTTCAGTTGTCTCGGCTACAAAATTCGAAGCCAAAGGCGGATAGCAGCATGAGAGTGGGAAGGAAAAAATTTGATCACATTGTCTTGGGACCGGCCGCTGGACAGGGCTTGCCTAATCGGATCCAATGTCAAGGTCTCAAGGCTGTCCAAAGAAGGCATCTGCCTTCTTTTCACTTATCTCTTTTGAAGGAAAAGATTTCCTTTGTCACCGTCTTTACCATCTACAACCAATCCTTACAAAATTCTTTTGATCAGAAAGTACCTACAAATGTAACGGTGGGGAACTCTACCTATGATAAGACAGGAAGGTCCATGGCAATTCTCAATGTCTTTGCCAACTTCATTAAGGTAGCAATGCCCAGAAGTAATATTTTTATTTTGACTGATCCTGCTAGCAACTTTCCTGCTGTTCCCAGCAATGCTGTTGTGATGCATATCCCAGGCGACTACTCACGAAACAACTTGATGCTTCAAAGGATCAAGTCTTACATTGACTTTTTGGAAGCAAGGCTATCAGGACATATTGGGAAGCAAAATCAGACTGATCATTTTATTTTCACAGATTCAGATATTGCAGTGGTTGACGACCTGACCGAGACTGTCAAACAACTACCCAGATTTTCACATAGGTCTTACTTTCCGAAATAATAAGGATCAGCCTCTCAATTCGGGCTTCATTTTAGTGAGAGGAACAGATGAGGCTATTTCAAAGGCAAAAGCCTTTTTGGAGGACGTTCTAGAGGTTTACAAGTCTATGTTCATGAAATCTGCTCGCATGCTTGGAGATCAGTTGGCTTTAGCTTGGATTGTAAAAAACCAACCTCTATTTGATGCCCAACGATTTAGGAATCCTAAAGCTTTTGTTGCTGAAGTTCATAGGGCACAAGTCCTCTTTTTACCATGTGCCATCTACAATTGGACTCCACCTGAAGGTTCAGGGCAATTTCATGGCATGCCTGAGGATGTCAAGGTTATTCATTTCAAAGGATCAAGGAAACGGCTTATGCTGGAAGCTTGGAACTTCTTCAACTCCCATCCTATTGATTTCTCGGACATGATGTGCTTAATTTTAAAGAGTGGAAGGGTGAAGTATGATTTCTAAGAAGCTTACATTTATCTGTGCCTTGAAACTCTTATTAATTGTGTAACCATGTAGATGGACTTAATCAAATCAGAAACAAAAGATTCGGAAATTGAGAGGTTTAAGAATCTTTTCAATGTTAAATTGAAGTCTAGAATTTGTTAGTATATTAATTTCTCATCCATGTTTTGGAAAATCAGTAAGATGTTTTTCCGGAGAATTGGCAATCTTCTTCGCATGAATGATACTAGTGCTTATGTATAGTTGTGATATTTCAAGAGAGCCCATTACACACCAGGCATATCACTAAAGGAGAAAATACATTGATATTGCAATATGACAAGCAAATAAGCAAGGTTCAGTATGAAACTATTATAGCATGCAGTAATAACTTGTTAAAGGATATTAGATTTAAGAGCCTCGCAAATCTTGGCTGCAAGCGACAAAGGAAAAAGTGGAAGGTGGTTTGATGCTGGCAAATTGTAGGCTCATCTATTGTGAAAAATTTCTGCAAATTATATGTTTAGGTGTTTATTCAGTGCAGCAAATGCATTCATTCTTTTGAAGTGGGAAGATAGAAGTATATCAAATGCTGATGTTTCTCAACTACGATCCTCATTCAAACAAATAAGGTGGCCTGAGGATCTATAACTTTATAGGGTGGAGACATGATCTGAACCAAAGGGAAGACGGGATAAGTATTTTGCCTTTGCTCTGCGGACATATTCAATGGAAAGCCGAAGCATTACCTTGTGTTTTGTGACTCAATAGAAAATTTGACCCGTGGATCCCTTTTATTTTCACTAGGGTACTGTTTATTTCTTGCAAAGCACTAGTGTGAAACATGGAAATAAGATGAAATTTGTATGAAGGTAGAAGTATAGCTGCTTCTGTTGCTATAGTTTCTGTAG

Ace file of the UniGene sp_v2.0_unigene5868

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Unassigned protein	-	-	-
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	89%
Blast2go	uncharacterized protein	-	-	80%

Source	GOs	Term	Type	Identity
Blast2go	tRNA splicing, via endonucleolytic cleavage and ligation	GO:0006388	Biological Process	80%
Blast2go	transferase activity, transferring phosphorus-containing groups	GO:0016772	Molecular Function	80%
Blast2go	mitochondrion	GO:0005739	Cellular Component	80%

Source	InterPros	Term	Type	e value	Identity
Sma3	Phosphotransferase KptA/Tpt1	IPR002745	-	0.0	-
Sma3	Protein of unknown function DUF1442	IPR009902	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5ABA0

Fln msg: Overlapping hits, possible frame ERROR between 821 and 808,

Fln protein: MASLGKWRFIRFLPFILVLPFIFSVFQLSRLQNSKPKADSSMRVGRKKFDHIVLGPAAGQGLPNRIQCQGLKAVQRRHLPSFHLSLLKEKISFVTVFTIYNQSLQNSFDQKVPTNVTVGNSTYDKTGRSMAILNVFANFIKVAMPRSNIFILTDPASNFPAVPSNAVVMHIPGDYSRNNLMLQRIKSYIDFLEARLSGHIGKQNQTDHFIFTDSDIAVVDDLTETVKxxxxxxHIGLTFRNNKDQPLNSGFILVRGTDEAISKAKAFLEDVLEVYKSMFMKSARMLGDQLALAWIVKNQPLFDAQRFRNPKAFVAEVHRAQVLFLPCAIYNWTPPEGSGQFHGMPEDVKVIHFKGSRKRLMLEAWNFFNSHPIDFSDMMCLILKSGRVKYDF*

Protein Length: 394

Fln nts: CGTCGTGACCTTGGCTGTCACTCAGGGGGTTTGTAACAGACGCAAGCCAAGCATCAGTACTCTCCAGTTTGAATTGTGCCGACTGTTGGCACAAGAGATATGATTTGTTTCTGTGATCAGGTCGA_-_ATGGCAAGCTTAGGGAAATGGCGTTTTATCAGATTTTTACCTTTCATCCTCGTACTGCCCTTTATATTTTCAGTTTTTCAGTTGTCTCGGCTACAAAATTCGAAGCCAAAGGCGGATAGCAGCATGAGAGTGGGAAGGAAAAAATTTGATCACATTGTCTTGGGACCGGCCGCTGGACAGGGCTTGCCTAATCGGATCCAATGTCAAGGTCTCAAGGCTGTCCAAAGAAGGCATCTGCCTTCTTTTCACTTATCTCTTTTGAAGGAAAAGATTTCCTTTGTCACCGTCTTTACCATCTACAACCAATCCTTACAAAATTCTTTTGATCAGAAAGTACCTACAAATGTAACGGTGGGGAACTCTACCTATGATAAGACAGGAAGGTCCATGGCAATTCTCAATGTCTTTGCCAACTTCATTAAGGTAGCAATGCCCAGAAGTAATATTTTTATTTTGACTGATCCTGCTAGCAACTTTCCTGCTGTTCCCAGCAATGCTGTTGTGATGCATATCCCAGGCGACTACTCACGAAACAACTTGATGCTTCAAAGGATCAAGTCTTACATTGACTTTTTGGAAGCAAGGCTATCAGGACATATTGGGAAGCAAAATCAGACTGATCATTTTATTTTCACAGATTCAGATATTGCAGTGGTTGACGACCTGACCGAGACTGTCAAAxxxxxxxxxxxxxxxxxxCACATAGGTCTTACTTTCCGAAATAATAAGGATCAGCCTCTCAATTCGGGCTTCATTTTAGTGAGAGGAACAGATGAGGCTATTTCAAAGGCAAAAGCCTTTTTGGAGGACGTTCTAGAGGTTTACAAGTCTATGTTCATGAAATCTGCTCGCATGCTTGGAGATCAGTTGGCTTTAGCTTGGATTGTAAAAAACCAACCTCTATTTGATGCCCAACGATTTAGGAATCCTAAAGCTTTTGTTGCTGAAGTTCATAGGGCACAAGTCCTCTTTTTACCATGTGCCATCTACAATTGGACTCCACCTGAAGGTTCAGGGCAATTTCATGGCATGCCTGAGGATGTCAAGGTTATTCATTTCAAAGGATCAAGGAAACGGCTTATGCTGGAAGCTTGGAACTTCTTCAACTCCCATCCTATTGATTTCTCGGACATGATGTGCTTAATTTTAAAGAGTGGAAGGGTGAAGTATGATTTCTAA___GAAGCTTACATTTATCTGTGCCTTGAAACTCTTATTAATTGTGTAACCATGTAGATGGACTTAATCAAATCAGAAACAAAAGATTCGGAAATTGAGAGGTTTAAGAATCTTTTCAATGTTAAATTGAAGTCTAGAATTTGTTAGTATATTAATTTCTCATCCATGTTTTGGAAAATCAGTAAGATGTTTTTCCGGAGAATTGGCAATCTTCTTCGCATGAATGATACTAGTGCTTATGTATAGTTGTGATATTTCAAGAGAGCCCATTACACACCAGGCATATCACTAAAGGAGAAAATACATTGATATTGCAATATGACAAGCAAATAAGCAAGGTTCAGTATGAAACTATTATAGCATGCAGTAATAACTTGTTAAAGGATATTAGATTTAAGAGCCTCGCAAATCTTGGCTGCAAGCGACAAAGGAAAAAGTGGAAGGTGGTTTGATGCTGGCAAATTGTAGGCTCATCTATTGTGAAAAATTTCTGCAAATTATATGTTTAGGTGTTTATTCAGTGCAGCAAATGCATTCATTCTTTTGAAGTGGGAAGATAGAAGTATATCAAATGCTGATGTTTCTCAACTACGATCCTCATTCAAACAAATAAGGTGGCCTGAGGATCTATAACTTTATAGGGTGGAGACATGATCTGAACCAAAGGGAAGACGGGATAAGTATTTTGCCTTTGCTCTGCGGACATATTCAATGGAAAGCCGAAGCATTACCTTGTGTTTTGTGACTCAATAGAAAATTTGACCCGTGGATCCCTTTTATTTTCACTAGGGTACTGTTTATTTCTTGCAAAGCACTAGTGTGAAACATGGAAATAAGATGAAATTTGTATGAAGGTAGAAGTATAGCTGCTTCTGTTGCTATAGTTTCTGTAG

Fln Alignment:

Contig5868___MASLGKWXXXXXXXXXXXXXXXXSVFQLSRLQNSKPKADSSMRVGRKKFDHIVLGPAAGQGLPNRIQCQGLKAVQRRHLPSFHLSLLKEKISFVTVFTIYNQSLQNSFDQKVPTNVTVGNSTYDKTGRSMAILNVFANFIKVAMPRSNIFILTDPASNFPAVPSNAVVMHIPGDYSRNNLMLQRIKSYIDFLEARLSGHIGKQNQTDHFIFTDSDIAVVDDLTETVKxxxxxHIGLTFRNNKDQPLNSGFILVRGTDEAISKAKAFLEDVLEVYKSMFMKSARMLGDQLALAWIVKNQPLFDAQRFRNPKAFVAEVHRAQVLFLPCAIYNWTPPEGSGQFHGMPEDVKVIHFKGSRKRLMLEAWNFFNSHPIDFSDMMCLILKSGRVKYDF
D5ABA0______________MASSGKWRFIRFLPFILILPFIFSGFQLSRLQNSKPKGDGSVGVGRKKFDYIVLGPAAGQGLPNRIQCQGLKAVKRRPLPSFHLSLVKEKISFVTVFTIYNQSLQISFDQKVSTNVSVGNSTYDKTQRSMAILNVFANFIKVAMPRSNIFILTDPASNFPVVPSNAVVMHIPGDYSRNNLMLQRIKSYIDFLEARLSGHIGKQNQVDHFIFTDSDIAVVDDLGDVVExxxxxHIGLTFRNNKDQPLNSGFILVRGTDEAVSKAKAFLEEVLEIYKSMFMKAARMLGDQLALAWIVKNQPLFDAQRFRNPKAFVAEVHRAQVLFLPCAIYNWTPPEGAGQFHGMPEDVKVIHFKGSRKRLMMESWNFFNSHPVDFSDMMCLILKSGRVKYDF

SNPs (tot: 38)

Position	A %	C %	G %	T %	Probability
476	33		66		1.0
566		33		66	0.997452
780	16		83		0.994512
1041		87		12	0.992554
1070		25		75	1.0
1072	70			30	1.0
1073	70	30			1.0
1077	30			70	1.0
1079	70	30			1.0
1081	70			30	1.0
1084		30	70		1.0
1085		70		30	1.0
1086	70		30		1.0
1087		70		30	1.0
1088	70	30			1.0
1090		30	70		1.0
1093	30	70			1.0
1112		10		90	0.90298
1113	10	90			0.90298
1115	90			10	0.90298
1189			90	10	0.90298
1192			10	90	0.90298
1193		90	10		0.90298
1292	75	25			0.996473
1296	75		25		0.996473
1302		25		75	0.996473
1305	75			25	0.996473
1306	75		25		0.996473
1308	75	25			0.996473
1309	75			25	0.996473
1313		25		75	0.996473
1318		25		75	0.996473
1319	25			75	0.996473
1360		50	50		1.0
1468	25	75			0.996473
1651		11		88	0.923564
1657		12		87	0.992554
1720		50		50	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain