SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene5847

Length: 1826 nt

Origin of reads: Biogeco3 v1 Uagpf v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene5847

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	DUF248 multi-domain protein	-	-	1.0e-09	26%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	62%
Blast2go	protein	-	-	0.0	72%

Source	ECs	Term	Type	e value	Identity
Blast2go	Transferases, Transferring one-carbon groups, Methyltransferases.	EC:2.1.1.-	-	0.0	72%

Source	KEGGs	Term	Identity
Blast2go	Ubiquinone and other terpenoid-quinone biosynthesis	00130	72%
Blast2go	Tetracycline biosynthesis	00253	72%
Blast2go	Histidine metabolism	00340	72%
Blast2go	Tyrosine metabolism	00350	72%
Blast2go	Phenylalanine metabolism	00360	72%
Blast2go	Tryptophan metabolism	00380	72%
Blast2go	Benzoxazinoid biosynthesis	00402	72%
Blast2go	Selenocompound metabolism	00450	72%
Blast2go	Biosynthesis of 12-, 14- and 16-membered macrolides	00522	72%
Blast2go	Polycyclic aromatic hydrocarbon degradation	00624	72%
Blast2go	Methane metabolism	00680	72%
Blast2go	Porphyrin and chlorophyll metabolism	00860	72%
Blast2go	Carotenoid biosynthesis	00906	72%
Blast2go	Phenylpropanoid biosynthesis	00940	72%
Blast2go	Flavonoid biosynthesis	00941	72%
Blast2go	Anthocyanin biosynthesis	00942	72%
Blast2go	Stilbenoid, diarylheptanoid and gingerol biosynthesis	00945	72%
Blast2go	Isoquinoline alkaloid biosynthesis	00950	72%
Blast2go	Insect hormone biosynthesis	00981	72%
Blast2go	Biosynthesis of type II polyketide products	01057	72%
Blast2go	Biosynthesis of alkaloids derived from shikimate pathway	01063	72%
Blast2go	Metabolic pathways	01100	72%
Blast2go	Biosynthesis of secondary metabolites	01110	72%

Source	GOs	Term	Type	e value	Identity
Blast2go	methylation	GO:0032259	Biological Process	0.0	72%
Blast2go	methyltransferase activity	GO:0008168	Molecular Function	0.0	72%

Source	InterPros	Term	Type	e value	Identity
Sma3	Pancreatic hormone-like	IPR001955	-	0.0	-
Sma3	Methyltransferase type 11	IPR013216	-	0.0	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LPA4

Fln msg: Unexpected STOP codon in 5 prime region,

Fln protein: PWLSLILLLVCSNLVTLLVFSGPNNWSGSHRGVGLLDLAQSDSLRLNFNELLWKLNTTQHDLLSTESELHTVKADLQTSNRISENLISELAHLTDFLKASFRGEGNAQAETDGGDPWGQWTEKNREQLPQELIEIVAGQKLPLGFNRAFNTDTVYIPIGHPCILLKDDLAKYMSYERGGTCPDDDNLAQQLMLRGCEPLPRRRCRKPIPEGYQEPHPFPESMWTTPPDTSILWTPYNCKSYGCLIDRKNRKDLIVDCKDCFDLQFREKYRWLNATSNDLNFAIDEVLGMKKGTIRIGLDIGGGTATFAVRMRERNVTIVTTSMNFDGPFNNFIASRGVVPMFVTISQRLPFFDSTLDLVHSMHVLSNWIPITMLEFVLFDIYRVLRPGGLFWLDHFFCLEPQLGELYVPLLERIGFNKLKWSIGRKMDRGIRFKEMYISALLEKPLSPRTSRRL*

Protein Length: 456

Fln nts: CGTCGTGACCTTGGCTGTCACTCAAAGTCCCCTTGTACCCCAATGGGCTCCTCCGACGCTAAGAAAGAAATGGAACCCACGTGCCGCAGGAGCGTCGTGACCTTGGCTGTCACTCATTCTGTTGCTCGTCTGCTCCAATTTGGTAACACTACTCGTCTTCTCCGGCCCAAACAACTGGTCCGGATCCCACAGAGGGGTTGGCCTCCTGGACCTTGCCCAATCGGATTCCTTGAGACTGAACTTCAATGAACTTCTCTGGAAGCTCAACACCACACAGCACGATCTCCTCTCGACCGAATCGGAGCTCCACACCGTGAAGGCCGACCTGCAGACCTCCAACCGGATCTCGGAAAATCTCATATCGGAGCTCGCCCACCTCACGGATTTCCTCAAAGCGTCGTTCCGAGGCGAAGGCAACGCGCAGGCGGAGACGGACGGCGGGGACCCGTGGGGTCAGTGGACGGAAAAGAACAGAGAGCAGCTGCCGCAGGAGCTGATTGAGATCGTTGCGGGGCAGAAGCTCCCGTTGGGCTTCAACCGCGCCTTCAATACGGACACCGTGTATATTCCGATCGGGCATCCGTGTATCCTTTTGAAGGATGATCTGGCGAAATACATGAGCTACGAACGTGGGGGTACTTGCCCGGACGACGATAATCTCGCCCAGCAACTCATGCTCCGGGGATGCGAACCTCTTCCCCGCAGGCGTTGTAGAAAACCCATCCCTGAAGGCTACCAGGAGCCACACCCATTCCCAGAGAGCATGTGGACTACACCTCCAGACACCAGCATTTTGTGGACTCCATACAACTGCAAGAGTTATGGCTGTCTCATAGATCGCAAGAATAGAAAGGATCTCATTGTGGACTGTAAAGATTGTTTCGATCTGCAGTTCCGTGAGAAGTACAGATGGCTGAATGCTACCAGCAACGATTTGAACTTCGCCATAGACGAAGTGTTGGGGATGAAGAAAGGAACTATTCGCATCGGCCTGGACATCGGCGGAGGAACAGCAACCTTCGCGGTGAGAATGCGCGAGAGAAATGTGACGATAGTGACCACTTCCATGAATTTTGACGGGCCCTTCAACAATTTCATAGCCTCTAGGGGAGTGGTGCCCATGTTCGTCACAATTTCTCAGCGCCTGCCCTTCTTTGACAGCACGCTCGACCTCGTTCATTCAATGCACGTCCTCAGCAACTGGATACCGATCACGATGCTGGAATTTGTACTGTTCGACATCTACAGGGTCCTCCGCCCTGGTGGACTGTTCTGGCTCGACCATTTCTTCTGTCTGGAGCCTCAGCTCGGGGAACTGTATGTCCCCTTGCTGGAAAGGATTGGATTTAACAAGCTCAAGTGGTCTATTGGGCGTAAGATGGACAGAGGGATCCGATTCAAGGAAATGTATATTTCGGCCCTATTGGAAAAACCGCTCTCTCCCAGGACGAGCAGACGTCTTTAA___TTTTTCTTTTCATTAAGCCCATGTTTCAAGCTTTCCTAAATTGATTACACTTCAGATGTAATAAAGGCTGAATATTAGTTTGCAGAATAGGAGGAGGAGAAGAGAACAGAGCAGGGAAAAAAATTCTGAAAAATGGTGTAAAAGAATGGAAGGAATCTGCAGAGCATATGAATCATGAATCACAGATTCATAATAATCCTCAGTTAGAAATGATTCCAGTCGTTGGAAGTGCTGGCTGAAACATGGATCAGATTTTATTTTTACGTTTTGCTAACGCTAATGTAATGAAGTAATATTTAAGCCTAGATTGTTTGCAGGTTTAAACAGTAAATAGAATGACTTTGATGATTTACCGTTAGAC

Fln Alignment:

Contig5847___NELLWKLNTTQHDLLSTESELHTVKADLQTSNRISENLISELAHLTDFLKASFRGEGNAQAETDGGDPWGQWTEKNREQLPQELIEIVAGQKLPLGFNRAFNTDTVYIPIGHPCILLKDDLAKYMSYERGGTCPDDDNLAQQLMLRGCEPLPRRRCRKPIPEGYQEPHPFPESMWTTPPDTSILWTPYNCKSYGCLIDRKNRKDLIVDCKDCFDLQFREKYRWLNATSNDLNFAIDEVLGMKKGTIRIGLDIGGGTATFAVRMRERNVTIVTTSMNFDGPFNNFIASRGVVPMFVTISQRLPFFDSTLDLVHSMHVLSNWIPITMLEFVLFDIYRVLRPGGLFWLDHFFCLEPQLGELYVPLLERIGFNKLKWSIGRKMDRGIRFKEMYISALLEKPLS
B8LPA4______________NDLITELTQTQRELLS-------IKTELKSSKQVSENLAAELTELIQFLRNPKSSNGG-----NADDPWLQWGEPNRVSLPKELKDFTAAKKLPLGRNPSFGSDTIYPPVGHACAVFKEDLNKYMDYKPGHRCPEDEILAQKLLLRGCEPLPRRRCHPATPSNYTEPFPFPESMWRTPPDSSVVWTAYSCKNYSCLIQRKYLK-VFDDCKDCFDLQGREKTRWLNGVGSDIDYSIDEVLKIKRHTIRIGLDIGGGTGTFAVRMRERNVTIITTSMNFNGPFNNFIASRGVVPMYISVSHRLPFFDNTLDIVHSMHVLSNWIPTVLLEFILYDINRILRPGGVFWLDRFFCIEDQL-KIYVPMIENVGFNKLKWSIGRKLDRGPALKEMYISALLEKPLN

SNPs (tot: 128)

Position	A %	C %	G %	T %	Probability
168		71	28		1.0
169		71		28	1.0
170	71	28			1.0
171	71		28		1.0
172	71			28	1.0
173		71	28		1.0
175	71	28			1.0
178			71	28	1.0
180			28	71	1.0
182		71		28	1.0
184		28	71		1.0
186		28		71	1.0
187		71		28	1.0
189	28	71			1.0
197		11	88		0.923564
200		11		88	0.923564
203	11	88			0.923564
207		88		11	0.923564
211	88	11			0.923564
212		88		11	0.923564
213		88	11		0.923564
215		11		88	0.923564
216	11		88		0.923564
219	11	88			0.923564
234	86	13			0.999973
236	86			13	0.999973
241	86	13			0.999973
245		86	13		0.999973
246	86		13		0.999973
247	86	13			0.999973
250	86	13			0.999973
378		86	13		0.999973
381	86		13		0.999973
382		86		13	0.999973
384		14	85		0.999981
385	85	14			0.999981
388			14	85	0.999981
389		85	14		0.999981
392		85	14		0.999981
393	85			14	0.999981
394	85	14			0.999981
401	14		85		0.999981
439			86	13	0.999973
443			84	15	0.999986
448		84		15	0.999986
452		16	83		0.999991
453	16		83		0.999991
454		16	83		0.999991
531		11	88		0.923564
533			88	11	0.923564
535			11	88	0.923564
538	88		11		0.923564
542			90	10	0.90298
543		90		10	0.90298
546		90	10		0.90298
547	10			90	0.90298
548		10		90	0.90298
550	90			10	0.90298
552			10	90	0.90298
553	90		10		0.90298
555			90	10	0.90298
557	90			10	0.90298
562	10		90		0.90298
615	90	10			0.90298
616			10	90	0.90298
617	90			10	0.90298
619	90		10		0.90298
623		10	90		0.90298
627		90		10	0.90298
629	88		11		0.923564
630	88	11			0.923564
631		88		11	0.923564
634	11		88		0.923564
635		11	88		0.923564
636			88	11	0.923564
637		11	88		0.923564
638	11		88		0.923564
652			88	11	0.923564
653	88			11	0.923564
656	88	11			0.923564
701		80	20		0.999996
702		70		30	1.0
706	70			30	1.0
708	30		70		1.0
710		30	70		1.0
714		30		70	1.0
715	70			30	1.0
717	66			33	1.0
718	62	37			1.0
720	62	37			1.0
721		62		37	1.0
723	37	62			1.0
814			22	77	0.999999
815			77	22	0.999999
817	77		22		0.999999
818	77			22	0.999999
821		22	77		0.999999
822		22		77	0.999999
1057	10		90		0.90298
1062		88		11	0.923564
1091	88		11		0.923564
1167		10	90		0.90298
1168		90		10	0.90298
1339	90	10			0.999898
1341			90	10	0.999898
1342	90	10			0.999898
1343			10	90	0.999898
1346	10		90		0.999898
1347	90	10			0.999898
1348		10		89	0.999919
1351	89		10		0.999919
1352	89		10		0.999919
1355	89			10	0.999919
1361	89	10			0.999919
1362			84	15	1.0
1363		10		89	0.999919
1398	85	14			1.0
1399			9	90	0.999872
1402	85	10		5	0.999793
1403	80	5		10	0.99967
1404			85	10	0.999793
1406	80	5			1.0
1407	85	5	10		0.999793
1408	85	15			1.0
1429		10	90		0.999898
1432	90			10	0.999898
1436		89		10	0.999919
1458	77		22		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain