SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene5720

Length: 1403 nt

Origin of reads: Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

UniGene Fasta

Ace file of the UniGene sp_v2.0_unigene5720

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	EF-hand, calcium binding motif-containing protein [Arabidopsis thaliana] dbj\|BAB10007.1\| unnamed protein product [Arabidopsis thaliana] gb\|AAL24286.1\| Unknown protein [Arabidopsis thaliana] gb\|AAN15353.1\| Unknown protein [Arabidopsis thaliana] gb\|AED91325	-	-	47%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	31%
Blast2go	protein	-	-	67%

Source	GOs	Term	Type	e value	Identity
Blast2go	plasma membrane	GO:0005886	Cellular Component	0.0	67%

Source	InterPros	Term	Type	Identity
Sma3	Calcium-binding EF-hand	IPR002048	-	-
Sma3	EF-hand-like domain	IPR011992	-	-
Sma3	EF-Hand 1, calcium-binding site	IPR018247	-	-
Sma3	EF-hand	IPR018248	-	-
Sma3	EF-HAND 2	IPR018249	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5AA92

Fln msg: W2: There is no M at the beginning, your sequence is longer than subject: 304 - 148

Fln protein: QNASYRVSGKGWHDDQDQSEAHDTWNELHSLDYGDEDYLNDHERFNITHRLLALFPLVDVKPLDGFISLAELEDWHVQIAVTRLLHRTDRELETVDANKDGLVTLKEYLRHLTDEELANNSTEHGEPGWWKEQFNCADEDGDGVLNHTEFYNFLHPGDSKNEKLLQWMCKERIRERDQDKDGKLNFEEFDHGAYELVKQYDEYDTRPTNITGHVPSSQERFNELDKNKDGFLTEDELGPILHKLHPGEISYAKYQAQYLMYQADENKDGQLTLEEMLHNPYAFYNTAYGDDEEDDYYMHDEFR*

Protein Length: 305

Fln nts: TACAGA_-_ATGCTTCGTATCGCGTGAGTGGTAAAGGATGGCACGATGACCAAGACCAGTCAGAAGCCCATGATACCTGGAACGAGCTCCATTCTCTCGACTATGGGGATGAAGATTACTTGAATGACCACGAGAGATTTAACATCACTCATCGGCTTTTAGCTCTTTTTCCACTTGTTGATGTGAAGCCCCTTGATGGTTTCATCAGTCTGGCAGAGCTGGAGGACTGGCATGTTCAGATTGCTGTCACGAGGTTGCTGCATCGCACGGACAGGGAGTTGGAGACCGTCGATGCCAACAAAGATGGGTTGGTCACACTCAAGGAATATCTCCGTCATCTTACAGACGAGGAATTAGCTAATAATAGCACGGAGCATGGTGAACCTGGCTGGTGGAAGGAACAATTTAATTGTGCAGATGAGGATGGTGATGGAGTTTTGAATCACACTGAGTTTTATAACTTTTTGCATCCTGGAGATAGTAAGAATGAGAAGCTACTTCAGTGGATGTGCAAAGAAAGAATTAGAGAGCGGGATCAAGATAAAGATGGCAAACTCAACTTTGAAGAATTTGATCATGGGGCATATGAACTCGTTAAGCAGTATGATGAATATGATACCAGGCCTACAAACATCACTGGACATGTACCATCTTCTCAAGAAAGATTTAATGAACTGGACAAGAACAAAGATGGATTCTTGACTGAGGATGAACTTGGGCCTATATTACACAAGTTACACCCTGGGGAAATTTCATATGCAAAATACCAGGCCCAGTATCTGATGTACCAGGCAGATGAGAACAAGGATGGCCAATTGACTCTAGAAGAAATGTTACACAATCCATATGCTTTCTACAACACTGCATATGGTGACGATGAGGAGGATGATTACTACATGCATGATGAATTCCGGTGATATTGCATAGATTTCTCGAGGATGGTTCTATATACATTTCAATTTCTTCTTCGGTAGCATCTGTATGACTAACTTATTGGACATGAAGTAAATTATAATTACTTGAGGCCATAATTGTCCACAAGTCAATGGAGGCACTTGTGAGGCACTTTTTTCTTATTTGGCTAATTTTTATGCTCATCTGCAGTGGCCAGAGAAGCACATAATTAATTTGAGTAGTACAGGGGCTCTTATTCCAGCTTCTCATGAATAGCAGACATTCTTTTTGAAATGCAGTAGATTCAGCATACATATATATTTGCGGTATATCATATTATTTATTATAAACTCGTGGATCCAGGAATAGAAATCCAAAGCCACTCCCACCAGTGGGAAATGTAAGTTAGGTTGAAAAGCTCCCATTTTCAGTATATTCCAAGCAAAATTTAATGCTTTGGAAATGGAACATATTGTTTGGGATTCTCAGAAATAATCCAATTTCAGCTTAAC

Fln Alignment:

Contig5720___TDRELETV----DANKDGLVTLKEYLRHLTDEELANNSTEHGEPGWWKEQFNCADEDGDGVLNHTEFYNFLHPGDSKNEKLLQWMCKERIRERDQDKDGKLNFEEF
D5AA92______________TEAELQDMINEADANGDGAIEFAEFVNLMAQNVKDTDSEEE-----LKEAFRAFDKDQNGFVSAEELHDVMI---NLGEKLTDEEIYEMIREADMDGDGQINYEEF

SNPs (tot: 65)

Position	A %	C %	G %	T %	Probability
89	85			14	0.993533
91			14	85	0.993533
94		85	14		0.993533
197		12	87		0.992554
199			12	87	0.992554
203	87	12			0.992554
209		90		10	0.90298
212			80	20	0.999996
214	80		20		0.999996
217		20	80		0.999996
222	80	20			0.999996
223			80	20	0.999996
225	80			20	0.999996
227	20			80	0.999996
228		20	80		0.999996
229			80	20	0.999996
289		14		85	0.993533
331	14	85			0.993533
333	14		85		0.993533
335		85		14	0.993533
369		42		57	1.0
421	20		80		0.995493
625	16	83			0.994512
645	20		80		0.995493
646		20		80	0.995493
648		80		20	0.995493
649		80		20	0.995493
766	66	33			0.997452
881	88			11	0.923564
923	11		88		0.923564
927	88			11	0.923564
930		11		88	0.923564
931			11	88	0.923564
934			11	88	0.923564
935		88		11	0.923564
938		11	88		0.923564
939		11	88		0.923564
940	88			11	0.923564
947	10			90	0.90298
948	80	20			0.999996
949			10	90	0.90298
955		10		90	0.90298
958		90	10		0.90298
959	90		10		0.90298
960	90	10			0.90298
962			10	90	0.90298
965		10		90	0.90298
968	10			90	0.90298
981		10	90		0.90298
1022		15		84	0.999986
1024	84			15	0.999986
1026	15		76	7	0.999983
1029	80		6	13	0.999961
1031	86		13		0.999973
1032	80	6	13		0.999961
1033		13		86	0.999973
1039	80			20	1.0
1059	35		64		1.0
1067		25		58	1.0
1097		50		50	1.0
1104	50		50		1.0
1105		50	50		1.0
1212			50	50	1.0
1317		57		42	1.0
1346	40		60		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain