SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene5619

Length: 2460 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene5619
CGTCGTGACCTTGGCTGTCACTCAATTTTGTGTAAAAGTATCTTGGTTCTCTTTACGAGCTTTAAAACAAATCAAATCTGAATCCGTCCGTGTACTCCGTTCCCTGATTCTCATGTCGAAAATGTAAAATACAAAAGCCTGAAAGCGGAGATTCAGGGTCGTCTCGCGAAAATAATGGATGTGATTCTATTCTCAATTATAATAGCAATAATAGTAATTTTCTTTATATTGGCAGTGATAATTACTATAGAGGGCACCTGCAAATCAAAATAATGATCAAGGCCGCAGTCCGAAATCTCATTTCGGTTTCCTGGTCGGCCCATAGGCTTACTCATTTTAATGGTTTTAGCTCTTTGGGAATTTAAGATCTGTTGAGAAAATTTGTGGGTTGATTTCAATATTCATTGGAAAGCAGTTTTTCGAGGTGAAAATGGGTAGGCACAGTGTAACGCAAAAGGAGAAGTTCATTCTCTGGGCAATATCTGTTATGTTTTTGGAGTTGATTATCATACGGAGAGCACTAGGTTCTGGTTCCTGCCCTTTCAGCAACGAGGTTTCTTCTGCAGATCATCACCATCACCATCACCACGGAGATCACAGTCATGGGGAAATTAATGGAATTGCAAGTGACGAGGACCATTCTGATCATCACGGGTGTGGACATTCCCATTTCCATGATCATGACAATGACCACCACGACCACCACCATGAACTTGACCACATTCCCAAATCATTCAGGCTTCCAGAAGAATTGAAAGAGGAGGAGGATCTGAGACTGCATGGATTTGGTTATGACTATCATGACCACGAAGACCATGACCATGAACACCTATCTACAACAGGTCTTTGGCTGCATGCAATGGGGTGCTCATTGCTAGTGAGCATGGCTTCACTAATCTGCCTTATGATCCTTCCTCTAATTCTCTTGCAAGGGAAGCCTTCTAAGACGGTGGTTGACTTGCTGGCAGCATTTGGGGCAGGAGCCATGTTAGGGGATTCCTTCTTTCACCAACTACCACATGCTCTTGGTGGCTCTCATAATCACTCCCATACTCATTCTTTGGATGGACATAATCACAATCACAATCACAACCACAACCAAGCTAGCATTGATTCTCATGAGCCTTCACATTCACATTCATTGGAGGAACTTTCAATTGGTCTTGCTGCTTTGGCGGGAATATTACTTTTTTTCCTTGTTGAGAAAGTTGTAAGGTATGTTGAAGAGCATTCATCACAAGGAGTACATGGTTTTCACCATGGCCATCATCATCATCATAAGAGTAATGTAGCTTCAAAGAAAGATGAGGACCAGCATGGTCCTAGCAGTACATTCATTGGTCAAAGAGAAAGCAGTGATTCTGATAAAGCACATGAATCAAAGTCATTGGATGCAAATGCTGATGCTGATACTGACACTGATGCTAATGGCAATGCAGCTGAACAAAAAGAAACCTACCGGAAATGATGCTTCGTAAGCGAAGCAAGTCTACAGGAGCTGGCAAGTTTGAGAAAGAATTTTTAAATTCTTCAGATGTTTCAAGCAAGATAGTGGAAACAGCAGCAGAGCCATCAATGCCTAGTTCAGCATCAAATATGGTGATTGGTTACTTGAATCTCTTTTCAGATGGAGTGCATAATTTTACGGATGGAATGGCTCTAGGAAGTGCATTCCTACTTCATGGATCAGTTGGTGGCTGGTCGCGGACACTATTTTTGCTTGCACATGAGCTTCCTCAGGAGATTGGGGATTTTGGAATACTGGTGGGATCAGGTTTCACTGTTTTCAAAGCACTTTTCTTCAATTTCCTATCGGCATTGGTCGCATTAGCAGGAACTGCATTGGCACTGTTAATAGGAAAAGATACAGGACATTCATCATTGATAGAGGGATTCACGGCTGGTGGATTTATCTATATTGCAATGGTTGGAGTGCTTCCTGAAATGCACCATAAAGGGACATCATTTAAAATTACCTTGCTTCAATTGATATCGTTGATGTCTGGAATGGGTGTGGCTCTGTGGATATCACTTATTGAGTGAGCAGCAAAGCAGGATGCTTTCGAAAGATAACATAGTATGTAAATTGCTAGGGAACCAAATTAAGGTATAAAAGCTGCCTGGCCTTTTCTGCAAAAGCTTCTACACCTTTAATTGATTTTCCTTCCATGCAAGTAATTTTCAACGAACACTGGATCACACATTCAGACCAAGATTTAAGCATGAAATATGCTTGAGTTAATAATACTTCCTGCAGCAATGCTGAAAGCGTTAGGCAGGTATTTTAAAGTCATCAAGTTCTATACTTTGGTTTTGTATCAAATTTAGAGTTATTTATTTTTTGATGCTTGCTAGTATAGCACCAGTTTTCTTTGAAATTTAAGTTTTTTTATAATCACTGCCCGCTTTACACCTTAGTAATGAGATTTATACATTGATTATATAATATCTCATATTCCC

Ace file of the UniGene sp_v2.0_unigene5619 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	[P] COG0428 Predicted divalent heavy-metal cations transporter	-	-	2.0e-13	34%
FL-Next	sp=IAA-alanine resistance protein 1; Arabidopsis thaliana (Mouse-ear cress).	-	-	0.0	64%
Blast2go	iaa-alanine resistance protein 1	-	-	0.0	79%

Source	GOs	Term	Type	Identity
Blast2go	metal ion transport	GO:0030001	Biological Process	79%
Blast2go	transmembrane transport	GO:0055085	Biological Process	79%
Blast2go	metal ion transmembrane transporter activity	GO:0046873	Molecular Function	79%
Blast2go	membrane	GO:0016020	Cellular Component	79%

Source	InterPros	Term	Type	e value	Identity
Sma3	Zinc/iron permease	IPR003689	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: sp_plants

Fln subject: Q9M647

Fln msg: Separated hits, possible frame ERROR between 1057 and 1474, your sequence is longer than subject: 538 - 469

Fln protein: MGRHSVTQKEKFILWAISVMFLELIIIRRALGSGSCPFSNEVSSADHHHHHHHGDHSHGEINGIASDEDHSDHHGCGHSHFHDHDNDHHDHHHELDHIPKSFRLPEELKEEEDLRLHGFGYDYHDHEDHDHEHLSTTGLWLHAMGCSLLVSMASLICLMILPLILLQGKPSKTVVDLLAAFGAGAMLGDSFFHQLPHALGGSHNHSHTxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxRKRSKSTGAGKFEKEFLNSSDVSSKIVETAAEPSMPSSASNMVIGYLNLFSDGVHNFTDGMALGSAFLLHGSVGGWSRTLFLLAHELPQEIGDFGILVGSGFTVFKALFFNFLSALVALAGTALALLIGKDTGHSSLIEGFTAGGFIYIAMVGVLPEMHHKGTSFKITLLQLISLMSGMGVALWISLIE*

Protein Length: 539

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTTTGTGTAAAAGTATCTTGGTTCTCTTTACGAGCTTTAAAACAAATCAAATCTGAATCCGTCCGTGTACTCCGTTCCCTGATTCTCATGTCGAAAATGTAAAATACAAAAGCCTGAAAGCGGAGATTCAGGGTCGTCTCGCGAAAATAATGGATGTGATTCTATTCTCAATTATAATAGCAATAATAGTAATTTTCTTTATATTGGCAGTGATAATTACTATAGAGGGCACCTGCAAATCAAAATAATGATCAAGGCCGCAGTCCGAAATCTCATTTCGGTTTCCTGGTCGGCCCATAGGCTTACTCATTTTAATGGTTTTAGCTCTTTGGGAATTTAAGATCTGTTGAGAAAATTTGTGGGTTGATTTCAATATTCATTGGAAAGCAGTTTTTCGAGGTGAAA_-_ATGGGTAGGCACAGTGTAACGCAAAAGGAGAAGTTCATTCTCTGGGCAATATCTGTTATGTTTTTGGAGTTGATTATCATACGGAGAGCACTAGGTTCTGGTTCCTGCCCTTTCAGCAACGAGGTTTCTTCTGCAGATCATCACCATCACCATCACCACGGAGATCACAGTCATGGGGAAATTAATGGAATTGCAAGTGACGAGGACCATTCTGATCATCACGGGTGTGGACATTCCCATTTCCATGATCATGACAATGACCACCACGACCACCACCATGAACTTGACCACATTCCCAAATCATTCAGGCTTCCAGAAGAATTGAAAGAGGAGGAGGATCTGAGACTGCATGGATTTGGTTATGACTATCATGACCACGAAGACCATGACCATGAACACCTATCTACAACAGGTCTTTGGCTGCATGCAATGGGGTGCTCATTGCTAGTGAGCATGGCTTCACTAATCTGCCTTATGATCCTTCCTCTAATTCTCTTGCAAGGGAAGCCTTCTAAGACGGTGGTTGACTTGCTGGCAGCATTTGGGGCAGGAGCCATGTTAGGGGATTCCTTCTTTCACCAACTACCACATGCTCTTGGTGGCTCTCATAATCACTCCCATACTCAxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTCGTAAGCGAAGCAAGTCTACAGGAGCTGGCAAGTTTGAGAAAGAATTTTTAAATTCTTCAGATGTTTCAAGCAAGATAGTGGAAACAGCAGCAGAGCCATCAATGCCTAGTTCAGCATCAAATATGGTGATTGGTTACTTGAATCTCTTTTCAGATGGAGTGCATAATTTTACGGATGGAATGGCTCTAGGAAGTGCATTCCTACTTCATGGATCAGTTGGTGGCTGGTCGCGGACACTATTTTTGCTTGCACATGAGCTTCCTCAGGAGATTGGGGATTTTGGAATACTGGTGGGATCAGGTTTCACTGTTTTCAAAGCACTTTTCTTCAATTTCCTATCGGCATTGGTCGCATTAGCAGGAACTGCATTGGCACTGTTAATAGGAAAAGATACAGGACATTCATCATTGATAGAGGGATTCACGGCTGGTGGATTTATCTATATTGCAATGGTTGGAGTGCTTCCTGAAATGCACCATAAAGGGACATCATTTAAAATTACCTTGCTTCAATTGATATCGTTGATGTCTGGAATGGGTGTGGCTCTGTGGATATCACTTATTGAGTGA___GCAGCAAAGCAGGATGCTTTCGAAAGATAACATAGTATGTAAATTGCTAGGGAACCAAATTAAGGTATAAAAGCTGCCTGGCCTTTTCTGCAAAAGCTTCTACACCTTTAATTGATTTTCCTTCCATGCAAGTAATTTTCAACGAACACTGGATCACACATTCAGACCAAGATTTAAGCATGAAATATGCTTGAGTTAATAATACTTCCTGCAGCAATGCTGAAAGCGTTAGGCAGGTATTTTAAAGTCATCAAGTTCTATACTTTGGTTTTGTATCAAATTTAGAGTTATTTATTTTTTGATGCTTGCTAGTATAGCACCAGTTTTCTTTGAAATTTAAGTTTTTTTATAATCACTGCCCGCTTTACACCTTAGTAATGAGATTTATACATTGATTATATAATATCTCATATTCCC

Fln Alignment:

Contig5619___RLPEELKEEEDLRLHGFGXXXXXXXXXXXXHLSTTGLWLHAMGCSLLVSMASXXXXXXXXXXXLQGKPSKTVVDXXXXXXXXXXXGDSFFHQLPHALGGSHNHSHTHxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxRKRSKSTGAGKFEKEFLNSSDVSSKIVETAAEPSMPSSASNMVIGYLNLFSDGVHNFTDGMALGSAFLLHGSVGGWSRTLFLLAHELPQEIGDFGILVGSGFTVFKALFFNFXXXXXXXXXXXXXXLIGKDTGHSSLIEGFTAGGFIYIAMVGVLPEMHHKGTS-FKITLLQLISLMSGMGVALWISLIE
Q9M647______________KLPEELAEEEDMRLCGFGPCLHDHDHESSSTLTGFALWLNALGCSLLVSLASLICLVLLPIMFVQGKPSKWFVDSLALFGAGAMLGDAFLHQLPHAFGGGHSHSNDHxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxRKTSASDATDKSDSGTEITSDGKSDKPE-----QVETRSSSLVFGYLNLFSDGVHNFTDGMALGSAFLIYGSVGGWSRTMFLLAHELPQEIGDFGILVRSGFTVTKALFFNFLSALVALAGTALVLVWGNEPGQSSLIEGFTAGGFIYIAVAGVLAEMNNSGKSTLKNSACHLISLILGMSVALCISLIE

SNPs (tot: 47)

Position	A %	C %	G %	T %	Probability
554		91		8	0.999807
560	9		90		0.999842
569		85	9	4	0.999726
570	9	85		4	0.999726
634	10			89	0.999919
1030		12		87	0.992554
1164	13			82	1.0
1189			91	8	0.999807
1238		16		83	1.0
1278	77		22		0.999999
1280		22		77	0.999999
1282			22	77	0.999999
1283		22		77	0.999999
1989	21	78			1.0
1990		78	21		1.0
1991	78			21	1.0
1994		21	78		1.0
1996	78		21		1.0
1997		21		78	1.0
1998		21	78		1.0
2001		78	21		1.0
2002			21	78	1.0
2005	25			75	1.0
2007		78	21		1.0
2008	21			78	1.0
2009		21		78	1.0
2010			21	78	1.0
2126	52	47			1.0
2160	10		89		0.999919
2171			20	80	1.0
2172	20			80	1.0
2174	20		80		1.0
2178		19		80	1.0
2179	19		80		1.0
2180	19	80			1.0
2183		80		19	1.0
2185	15			84	1.0
2186		15		84	1.0
2191	84		15		1.0
2199		10	90		0.999898
2238	90	10			0.90298
2239		11		88	0.923564
2240	87	12			0.992554
2241	87	12			0.992554
2243			87	12	0.992554
2258		33		66	1.0
2306	66	33			1.0

SSRs (tot: 3)

Start position	End position	Sequence	Length
1182	1196	ATG ATG ATG ATG ATG	15
1369	1388	TTGTGA TTGTGA TTGTGA TT	20
1873	1892	TGGTGA TGGTGA TGGTGA TG	20

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain