SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene5230

Length: 2237 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene5230
ATGTTCCATTAAATACAGCGTTGTTATCTTTCTGTTACCTTCCTCTCTGCTGAATATGCTATTGTTTCTGTTGCTTGGATAACAACGTAACAATAATAATGAGTAGCATTTTAGGCATTGTTCATCCTGGAATTCCCCATTCCCACCAAGACCAATTCCGATGACCCGTTCTTTTGTATATATAGATCTCTCATTTCCATGTATGTCTTTGAGTGTATCGGAAAGGCAGGCGCCGTTTCATTATTGGATCCTTTCAACACAGACAACAATCTTCTGGACAAAACGATGACATTTTTCCCGCAACATTCAGTACAAATAACTGATTAATCATTTCTGCAAATATTCATAGTGATTGTAGCCATATTTGGAATGGGATGCATGCTACAGTTTCTTTAAAAATGGATGATATTGCTGGTTCAAGAACTCAGAGAAATGTTGGCCAGACTCAGATAGAATTGGGATTGTCAGATTATGACAGAGACAATGAAGATGAGGATCAGATCCTTATAATCCCTTCAGATGCCGATAGAAAGGACCATGAATTCAAGTCTACAATGAATGGACCATTGGTGGATGTCTTGCACATGTGGACATTGAAAAGAGTGAAAAATCGAATATTTGGAAGCATTCGCATTGTCACAATGCAAAACAAAATAAATCTGTTGCTTCCTTTTGGTCCTGCTGCAGTGATTTTAGCTCATTTTTCGAAGGGAGAACATGGATGGATTTTCTTTTTCAGCTTGTTGGGCATAACACCTTTGGCTGAGCGACTAGGCTTTGTAACAGAGCAGCTAGCATTTTTCACTGGTCCCACAGTTGGAGGCCTTCTGAATGCTACATTTGGAAACGCGACTGAGATGATCGTCTCTTTATATGCTCTTGATAACGGAATGGTTCGCATTGTGCAACAATCTTTACTTGGTTCTATCTTGTCAAACATGTTGCTTGTGCTTGGATGTGCCTTTTTCATGGGTGGTTTTATTCATCATCAGAAAGAACAGGTCTTTAATAAGGCTGCTGCTGTTGTCAACTCAGGATTATTATTGATGGCTGTTATGGGCCTACTTTTCCCAGCTGTACTGCACTATACACACACAGAATTGCATGTTGGAAAATCAGAATTATCCCTTTCAAGGTTTAGCAGCTGTGTCATGCTTGTAGCCTATGTATTCTACCTTGTCTTCCAGCTGAAGAGTCACAGAAATCTTTATGACCCGGTAGATCAGACTCAGGGAGAGAATGGGGGAGTTACAAATGAAGAGGAAGTTGCTGTGATAACCCATTGGGAAGCAATATCATGGCTTGCTATCCTTACTGCTTGGATTTCTTTTCTCTCAGGATATCTTGTAAATGCCATAGAGGGGGCATCTCAGTCTTGGAATATGCCAGTTGCTTTTATAAGTGTGATTCTTCTACCAATTGTTGGGAATGCTGCGGAGCATGCAAGTGCTATAATGTTTGCTATGAAAGATAAGCTGGACATCTCCCTAGGTGTTGCGATTGGGTCTTCAACACAAATAGCGATGTTTGTGATTCCCTTTTGTGTGGTGGTTGGTTGGATTATGGGAAAGTCCATGGATTTGAACTTTCAACTTTTTGAGACTGCTACATTATTTATTACAGTCATCGTGGTCGCATTTATGCTTCAGGAAGGTACATCAAACTATTTCAAAGGGTTAATGCTTATTTTATGCTACATGATAGTTGCTGCAAGCTTCTTTGTACATGTCGATCCTGAACACACTGAACACGATAATACTTAGCGGTGTTCTTGAGAATCACTTGCAGGATTCAGAAGCTGAGGTATCTTATTATATTATGGAAGGGATTTATATCAAATTATCCATGTGTATTGATTATATATGCAAGCATGCATTATCAGCGTTGGCTCAGATTTGGGGAAGAACAGTTAATGTTGCTCAATGTTCTTTTTTTGTCAGGACCAATGAACCTGGGTTTTTAAATTACAGGCTTCAACTTTAGGGATTCCAATTAAGTGCCTTGTAGATTGCTCTCAGAGGTTTGCATAGGATTATAGCTTCTATAATTTGTGTTGTAATGGGATTGGGGCTCTTGGGAATTCAGAAGATCAGCACCCTGAATTCCCTGTGGTGTTAGTTGACAGCAGTTGGATTGGGGAAATGTATATTCTGTTTAAGGAATGAAATAGTGACAATTCCAGCAACATGAGCTTTTTGCCTTATATGGAAATATAGAAAAGTAATGATCTTAGAAAA

Ace file of the UniGene sp_v2.0_unigene5230 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	H+/Ca2+ exchanger 2 [Ipomoea nil]	-	-	78%
FL-Next	sp=Vacuolar cation/proton exchanger 2; Oryza sativa subsp. japonica (Rice).	-	-	75%
Blast2go	vacuolar cation proton exchanger 2-like	-	-	84%

Source	GOs	Term	Type	Identity
Blast2go	cation transport	GO:0006812	Biological Process	84%
Blast2go	transmembrane transport	GO:0055085	Biological Process	84%
Blast2go	cation transmembrane transporter activity	GO:0008324	Molecular Function	84%
Blast2go	integral to membrane	GO:0016021	Cellular Component	84%

Source	InterPros	Term	Type	Identity
Sma3	Calcium/proton exchanger	IPR004713	-	-
Sma3	Calcium/proton exchanger superfamily	IPR004798	-	-
Sma3	Sodium/calcium exchanger membrane region	IPR004837	-	-
Sma3	Phosphopantetheine attachment site	IPR006162	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: sp_plants

Fln subject: Q5KQN0

Fln msg:

Fln protein: MHATVSLKMDDIAGSRTQRNVGQTQIELGLSDYDRDNEDEDQILIIPSDADRKDHEFKSTMNGPLVDVLHMWTLKRVKNRIFGSIRIVTMQNKINLLLPFGPAAVILAHFSKGEHGWIFFFSLLGITPLAERLGFVTEQLAFFTGPTVGGLLNATFGNATEMIVSLYALDNGMVRIVQQSLLGSILSNMLLVLGCAFFMGGFIHHQKEQVFNKAAAVVNSGLLLMAVMGLLFPAVLHYTHTELHVGKSELSLSRFSSCVMLVAYVFYLVFQLKSHRNLYDPVDQTQGENGGVTNEEEVAVITHWEAISWLAILTAWISFLSGYLVNAIEGASQSWNMPVAFISVILLPIVGNAAEHASAIMFAMKDKLDISLGVAIGSSTQIAMFVIPFCVVVGWIMGKSMDLNFQLFETATLFITVIVVAFMLQEGTSNYFKGLMLILCYMIVAASFFVHVDPEHTEHDNT*

Protein Length: 464

Fln nts: ATGgtCCATTAAATACAGCGcaGgtAcCTTaCTGgtACCTTCCTCTCTGCTGAATATGCTATTGTTTCTGTTGCTTGGATAACAACGTAACAATAATAATGAGTAGCATTTTAGGCATTGTTCATCCTGGAATTCCCCATTCCCACCAAGACCAATTCCGATGACCCGTTCTTTTGTATATATAGATCTCTCATTTCCATGTATGTCTTTGAGTGTATCGGAAAGGCAGGCGCCGTTTCATTATTGGATCCTTTCAACACAGACAACAATCTTCTGGACAAAACGATGACATTTTTCCCGCAACATTCAGTACAAATAACTGATTAATCATTTCTGCAAATATTCATAGTGATTGTAGCCATATTTGGAATGGG_-_ATGCATGCTACAGTTTCTTTAAAAATGGATGATATTGCTGGTTCAAGAACTCAGAGAAATGTTGGCCAGACTCAGATAGAATTGGGATTGTCAGATTATGACAGAGACAATGAAGATGAGGATCAGATCCTTATAATCCCTTCAGATGCCGATAGAAAGGACCATGAATTCAAGTCTACAATGAATGGACCATTGGTGGATGTCTTGCACATGTGGACATTGAAAAGAGTGAAAAATCGAATATTTGGAAGCATTCGCATTGTCACAATGCAAAACAAAATAAATCTGTTGCTTCCTTTTGGTCCTGCTGCAGTGATTTTAGCTCATTTTTCGAAGGGAGAACATGGATGGATTTTCTTTTTCAGCTTGTTGGGCATAACACCTTTGGCTGAGCGACTAGGCTTTGTAACAGAGCAGCTAGCATTTTTCACTGGTCCCACAGTTGGAGGCCTTCTGAATGCTACATTTGGAAACGCGACTGAGATGATCGTCTCTTTATATGCTCTTGATAACGGAATGGTTCGCATTGTGCAACAATCTTTACTTGGTTCTATCTTGTCAAACATGTTGCTTGTGCTTGGATGTGCCTTTTTCATGGGTGGTTTTATTCATCATCAGAAAGAACAGGTCTTTAATAAGGCTGCTGCTGTTGTCAACTCAGGATTATTATTGATGGCTGTTATGGGCCTACTTTTCCCAGCTGTACTGCACTATACACACACAGAATTGCATGTTGGAAAATCAGAATTATCCCTTTCAAGGTTTAGCAGCTGTGTCATGCTTGTAGCCTATGTATTCTACCTTGTCTTCCAGCTGAAGAGTCACAGAAATCTTTATGACCCGGTAGATCAGACTCAGGGAGAGAATGGGGGAGTTACAAATGAAGAGGAAGTTGCTGTGATAACCCATTGGGAAGCAATATCATGGCTTGCTATCCTTACTGCTTGGATTTCTTTTCTCTCAGGATATCTTGTAAATGCCATAGAGGGGGCATCTCAGTCTTGGAATATGCCAGTTGCTTTTATAAGTGTGATTCTTCTACCAATTGTTGGGAATGCTGCGGAGCATGCAAGTGCTATAATGTTTGCTATGAAAGATAAGCTGGACATCTCCCTAGGTGTTGCGATTGGGTCTTCAACACAAATAGCGATGTTTGTGATTCCCTTTTGTGTGGTGGTTGGTTGGATTATGGGAAAGTCCATGGATTTGAACTTTCAACTTTTTGAGACTGCTACATTATTTATTACAGTCATCGTGGTCGCATTTATGCTTCAGGAAGGTACATCAAACTATTTCAAAGGGTTAATGCTTATTTTATGCTACATGATAGTTGCTGCAAGCTTCTTTGTACATGTCGATCCTGAACACACTGAACACGATAATACTTAG___CGGTGTTCTTGAGAATCACTTGCAGGATTCAGAAGCTGAGGTATCTTATTATATTATGGAAGGGATTTATATCAAATTATCCATGTGTATTGATTATATATGCAAGCATGCATTATCAGCGTTGGCTCAGATTTGGGGAAGAACAGTTAATGTTGCTCAATGTTCTTTTTTTGTCAGGACCAATGAACCTGGGTTTTTAAATTACAGGCTTCAACTTTAGGGATTCCAATTAAGTGCCTTGTAGATTGCTCTCAGAGGTTTGCATAGGATTATAGCTTCTATAATTTGTGTTGTAATGGGATTGGGGCTCTTGGGAATTCAGAAGATCAGCACCCTGAATTCCCTGTGGTGTTAGTTGACAGCAGTTGGATTGGGGAAATGTATATTCTGTTTAAGGAATGAAATAGTGACAATTCCAGCAACATGAGCTTTTTGCCTTATATGGAAATATAGAAAAGTAATGATCTTAGAAAA

Fln Alignment:

Contig5230___RIFGSIRIVTMQNKINLLLPFGPAAVILAHFSKGEHGWIFFFSLLGITPLAERLGFVTEQLAFFTGPTVGGLLNATFGNATEMIVSLYALDNGMVRIVQQSLLGSILSNMLLVLGCAFFMGGFIHHQKEQVFNKAAAVVNSGLLLMAVMGLLFPAVLHYTHTELHVGKSELSLSRFSSCVMLVAYVFYLVFQLKSHRNLYDPVDQTQXXXXXXXXXXXVAVITHWEAISWLAILTAWISFLSGYLVNAIEGASQSWNMPVAFISVILLPIVGNAAEHASAIMFAMKDKLDISLGVAIGSSTQIAMFVIPFCVVVGWIMGKSMDLNFQLFETATLFITVIVVAFMLQEGTSNYFKGLMLILCYMIVAASFFVHVDPEHTEH
Q5KQN0______________RALTSVRVVILQAKINVLLPFGPLAVMLHYLSANHQGWVFLFSLIGITPLAERLGYATEQLALYTGPTIGGLLNATFGNATEMIISLYALKNGMIRVVQQSLLGSILSNMLLVLGCAFFAGGLVHPSRDQVFNKASAVVNSGLLLMAVLGLMFPAVLHFTHSEVQYGKSEVSLSRFSSCIMLVAYASYLFFQLKSQRSLYSPIGEQEEEVTEDEEEEKE--ITQGEAICWLFVLTIWISILSGYLVDAIQGASESLNMPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFVIPFCVVIGWIMGQQMDLNFQLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVAASFFVHVDPDSSNN

SNPs (tot: 94)

Position	A %	C %	G %	T %	Probability
310	88		11		0.923564
417		88	11		0.923564
418	11	88			0.923564
419	88		11		0.923564
421	88		11		0.923564
423		11		88	0.923564
426	88		11		0.923564
427	88	11			0.923564
428		11		88	0.923564
432	88		11		0.923564
436	11	88			0.923564
437		11		88	0.923564
438		77	22		0.999999
441		87		12	0.992554
442			12	87	0.992554
443	12			87	0.992554
445	12			87	0.992554
447	87	12			0.992554
449	12			87	0.992554
481		10		90	0.90298
494	90	10			0.90298
495		10	90		0.90298
496		10		90	0.90298
497	10		90		0.90298
526		12		87	0.992554
539	11	88			0.923564
592			90	10	0.90298
594	90		10		0.90298
599	88			11	0.923564
600	88			11	0.923564
602		11		88	0.923564
603	12		87		0.992554
604	12	87			0.992554
608		87		12	0.992554
610		87		12	0.992554
611		12	87		0.992554
612	87	12			0.992554
616		87	12		0.992554
617	12			87	0.992554
702			85	14	0.993533
705	87		12		0.992554
707		87	12		0.992554
711	87		12		0.992554
712	87	12			0.992554
715	12			87	0.992554
716		87	12		0.992554
719	12			87	0.992554
720	87	12			0.992554
721			12	87	0.992554
722	12			87	0.992554
723		12		87	0.992554
818	85	14			0.993533
1092		87		12	0.992554
1218	33		66		0.997452
1221			66	33	0.997452
1222		66		33	0.997452
2010		85		14	0.999981
2022	21			78	1.0
2117		12	87		0.992554
2118	87	12			0.992554
2119	87	12			0.992554
2122	90			10	0.90298
2134	11	88			0.923564
2136	88		11		0.923564
2141	87	12			0.992554
2142		12	12	75	0.975346
2143		25		75	1.0
2146	12			87	0.992554
2147			14	85	0.993533
2148	85		14		0.993533
2150		14		85	0.993533
2151	14		85		0.993533
2152		14		85	0.993533
2153			14	85	0.993533
2154	85	14			0.993533
2156		14	85		0.993533
2157		16		83	0.994512
2187	80	20			0.995493
2188		20		80	0.995493
2189		20		80	0.995493
2192			80	20	0.995493
2200		25	75		0.996473
2201	75	25			0.996473
2202	75	25			0.996473
2204		25	75		0.996473
2206	25		75		0.996473
2208			33	66	0.997452
2209	33	66			0.997452
2213		33	66		0.997452
2218			33	66	0.997452
2220		33	66		0.997452
2221	33	66			0.997452
2223			33	66	0.997452
2224	33			66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain