SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene4818

Length: 2545 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene4818
GATTTCTGATCGTTTTTAGTTCACACGTGCTGCCTCTTTATTTCGGAAGAATCTGTGTGTTTTGTTAGTTTTTGCCATTGGATATCAATGATTTTCTTTCAGTGCTCAGCTTTCTCCCATTTCTGTGGGGAAAATTAAAACTCTGAAGGTTGCAGTTAGTAGAATTTTAAGTATTCAGGTGTCCAAGGACAGTTCGATGGTTAGACAGCTGTATTTTTAAGGGCCGTCCAATTTGATCCTTTATCCTAATATTTAAGGAGCACAACCTTTTAGTATTAATGTCGGTGATTGACTATTTCCATATATGTAAAATGTTCATGTTTAAATAAATAGTGTAGACTGTGATTTGTGGTCTGGTTATGCAAGGTCTAACCAATATATTAATGAATCAACGTCTGCATGCACATATTCAACAGGCTCTCGAGTAATGTAAAGCCTATTTCCTTCGAAGAACAGTGAGGCGTTATCTTTGTGACTTTAGAAGGAACTGCAGATTCCATTAATACCTGCAGAAATGTGGAACTCGATTACGACCTCAACGGACATTCCTCAAGAATCAAGCACGAGGAACACAGACCTGTTCGCGACTGCCACTGAAAATGGCACTTCTGATTATTCACAAATTCCTGAAAAGCCAAGACCAATCAATCTGGAGCATAAAAGGTCACTTGATGAAAGGTCATTAAGTGAACTTCTGTCCCCTAGCTTAATTTCTCCACGACCATCTTCGAGGACCATGGAAACGTTTAGAAGCTTAGATGAAAATCGGTTTTCATCAGCTCAGAATACGCCTCGTTCAGCACGAGCTTCCTCTGAGCCTAATCCTATGATTGCTGATGCTTGGGAGGCTTTACGGAGATCACTGGTTTACTTTCGAGGAAAGCCTGTTGGAACTATTGCTGCTTTGGACCCTACAGAGGATTCACTAAACTATAATCAGGTATTTGTAAGAGACTTTTTTCCAAGTGCTTTGGCATTCTTGATGAATGGCGAACCTGAGATCGTCAAGCATTTCCTCTTGAAGACACTCCGACTTCAAGCATGGTGGGAGAAACGAGTGGATTGTTTTACATTAGGAGAAGGAGTTATGCCTGCCAGTTTTAAAGTACTCCATGATCCTGTTCGTAACAGTGATACAATGATTGCAGATTTTGGAGAAAGTGCGATAGGCAGAGTTGCTCCCGTAGATTCTGGATTTTGGTGGATTATTCTGTTGCGTGCCTATACAAAGTCTACTGGAGATCATTCTTTAGCAGACATGCCTGACTGCCAGCGTGGAATGAGGCTTATTTTGACATTGTGCCTCTCTGAAGGCTTCGACACTTTTCCCACTCTCCTCTGTGCAGATGGATGCTGTATGGTTGATCGCAGAATGGGCATTTATGGTTATCCCATTGAAATTCAAGCCTTGTTCTTCATGGCACTAAGGTGTGCCCGGATACTCCTTAAACCAGAAGGTGGGGGAAAAGAGTTCATAGAGCGAATTGATAAAAGGCTGCATGCATTAAGTTATCACATGCGTAACTATTTCTGGTTAGACTTTGAGCAGTTGAACAACATATATCGATACAAGACTGAGGAATATTCTCACACTGCTGTAAATAAATTCAATGTTATTCCTGATTCCATTACAGATTGGGTATTTGATTTCATGCCAATTAGAGGAGGTTATTTTATTGGAAATGTCAGCCCAGCTCGGATGGACTTTAGGTGGTTTTGTTTGGGTAATTGTATTGCAATTTTGTCATCCTTGGCAACACCTGAACAGTCTACAAGAATAATGGATCTTATTGAAGAGCGGTGGGAAGATCTGGTTGGAGAGATGCCTCTCAAGGTTTCATACCCTGCCTTAGAGGGTCATGAATGGCGAATAGTAACAGGTTGCGATCCAAAGAACACAAGATGGAGTTACCACAATGGAGGATCTTGGCCAGTGCTTTTGTGGCTATTCACTGCAGCCTGTATTAAAACGGGAAGGCCGCAAATGGCCAGAAGGGCCATTGAACTTGCTGAGACCAGACTATCTAAGGATGGCTGGCCTGAATATTATGATGGGAAGCTTGGACGATATATTGGAAAGCAGTCTCGAAAGTGGCAAACCTGGTCAGTTGCTGGATATCTTGTAGCCAAGATGATGCTTGAAGATCCATCCCATTTAGGTATGATAGCATTGGAAGAGGACAAGAAGATGAAGCCGTCCCTCACTCGATCAGCTTCTTGGATAATGTAAAATGGGGAAATCCGCCACTCTTGAATGTTTCGTCACTTCTGTATGACAAATGAGGCAATTCATAGCACATGTTGTGCAAGCATTCTCTAGCATTTTCATTCTGGTTGGTCTTAGTCGGGCCAAACTAGAAACTGCAAATAGCTCCTCCTATAATCAGATCAACTGGACAAAGCACAGTATCTTATGATGCGTCAGTCTCGTGGTATTTCAGCTCGTGTCTCATTTTCATTTCAAATAATCCCATATTCCAAGTTTTTCTGGAGTGTGTAATGGAACCAAGCAAATGTAAATGTGACTGTAATCAGTTTAATCGCT

Ace file of the UniGene sp_v2.0_unigene4818 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	neutral invertase-like protein [Arabidopsis thaliana] ref\|NP_001031790.1\| neutral invertase-like protein [Arabidopsis thaliana] emb\|CAB45447.1\| invertase-like protein [Arabidopsis thaliana] emb\|CAB80203.1\| invertase-like protein [Arabidopsis thaliana] gb\|	-	-	72%
FL-Next	tr=Putative uncharacterized protein; Selaginella moellendorffii (Spikemoss).	-	-	86%
Blast2go	protein	-	-	89%

Source	ECs	Term	Type	e value	Identity
Blast2go	Beta-fructofuranosidase.	EC:3.2.1.26	-	0.0	89%

Source	KEGGs	Term	Type	Identity
Blast2go	Galactose metabolism	00052		89%
Blast2go	Starch and sucrose metabolism	00500		89%
Blast2go	Metabolic pathways	01100		89%
Blast2go	Sucrose alpha-glucosidase.	EC:3.2.1.48	-	89%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Starch and sucrose metabolism	00500		0.0	89%
Blast2go	Metabolic pathways	01100		0.0	89%

Source	GOs	Term	Type	Identity
Blast2go	metabolic process	GO:0008152	Biological Process	89%
Blast2go	sucrose alpha-glucosidase activity	GO:0004575	Molecular Function	89%
Blast2go	binding	GO:0005488	Molecular Function	89%

Source	InterPros	Term	Type	e value	Identity
Sma3		IPR006937	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: tr_plants

Fln subject: D8RY26

Fln msg:

Fln protein: MWNSITTSTDIPQESSTRNTDLFATATENGTSDYSQIPEKPRPINLEHKRSLDERSLSELLSPSLISPRPSSRTMETFRSLDENRFSSAQNTPRSARASSEPNPMIADAWEALRRSLVYFRGKPVGTIAALDPTEDSLNYNQVFVRDFFPSALAFLMNGEPEIVKHFLLKTLRLQAWWEKRVDCFTLGEGVMPASFKVLHDPVRNSDTMIADFGESAIGRVAPVDSGFWWIILLRAYTKSTGDHSLADMPDCQRGMRLILTLCLSEGFDTFPTLLCADGCCMVDRRMGIYGYPIEIQALFFMALRCARILLKPEGGGKEFIERIDKRLHALSYHMRNYFWLDFEQLNNIYRYKTEEYSHTAVNKFNVIPDSITDWVFDFMPIRGGYFIGNVSPARMDFRWFCLGNCIAILSSLATPEQSTRIMDLIEERWEDLVGEMPLKVSYPALEGHEWRIVTGCDPKNTRWSYHNGGSWPVLLWLFTAACIKTGRPQMARRAIELAETRLSKDGWPEYYDGKLGRYIGKQSRKWQTWSVAGYLVAKMMLEDPSHLGMIALEEDKKMKPSLTRSASWIM*

Protein Length: 573

Fln nts: GATTTCTGATCGTTTTTAGTTCACACGTGCTGCCTCTTTATTTCGGAAGAATCTGTGTGTTTTGTTAGTTTTTGCCATTGGATATCAATGATTTTCTTTCAGTGCTCAGCTTTCTCCCATTTCTGTGGGGAAAATTAAAACTCTGAAGGTTGCAGTTAGTAGAATTTTAAGTATTCAGGTGTCCAAGGACAGTTCGATGGTTAGACAGCTGTATTTTTAAGGGCCGTCCAATTTGATCCTTTATCCTAATATTTAAGGAGCACAACCTTTTAGTATTAATGTCGGTGATTGACTATTTCCATATATGTAAAATGTTCATGTTTAAATAAATAGTGTAGACTGTGATTTGTGGTCTGGTTATGCAAGGTCTAACCAATATATTAATGAATCAACGTCTGCATGCACATATTCAACAGGCTCTCGAGTAATGTAAAGCCTATTTCCTTCGAAGAACAGTGAGGCGTTATCTTTGTGACTTTAGAAGGAACTGCAGATTCCATTAATACCTGCAGAA_-_ATGTGGAACTCGATTACGACCTCAACGGACATTCCTCAAGAATCAAGCACGAGGAACACAGACCTGTTCGCGACTGCCACTGAAAATGGCACTTCTGATTATTCACAAATTCCTGAAAAGCCAAGACCAATCAATCTGGAGCATAAAAGGTCACTTGATGAAAGGTCATTAAGTGAACTTCTGTCCCCTAGCTTAATTTCTCCACGACCATCTTCGAGGACCATGGAAACGTTTAGAAGCTTAGATGAAAATCGGTTTTCATCAGCTCAGAATACGCCTCGTTCAGCACGAGCTTCCTCTGAGCCTAATCCTATGATTGCTGATGCTTGGGAGGCTTTACGGAGATCACTGGTTTACTTTCGAGGAAAGCCTGTTGGAACTATTGCTGCTTTGGACCCTACAGAGGATTCACTAAACTATAATCAGGTATTTGTAAGAGACTTTTTTCCAAGTGCTTTGGCATTCTTGATGAATGGCGAACCTGAGATCGTCAAGCATTTCCTCTTGAAGACACTCCGACTTCAAGCATGGTGGGAGAAACGAGTGGATTGTTTTACATTAGGAGAAGGAGTTATGCCTGCCAGTTTTAAAGTACTCCATGATCCTGTTCGTAACAGTGATACAATGATTGCAGATTTTGGAGAAAGTGCGATAGGCAGAGTTGCTCCCGTAGATTCTGGATTTTGGTGGATTATTCTGTTGCGTGCCTATACAAAGTCTACTGGAGATCATTCTTTAGCAGACATGCCTGACTGCCAGCGTGGAATGAGGCTTATTTTGACATTGTGCCTCTCTGAAGGCTTCGACACTTTTCCCACTCTCCTCTGTGCAGATGGATGCTGTATGGTTGATCGCAGAATGGGCATTTATGGTTATCCCATTGAAATTCAAGCCTTGTTCTTCATGGCACTAAGGTGTGCCCGGATACTCCTTAAACCAGAAGGTGGGGGAAAAGAGTTCATAGAGCGAATTGATAAAAGGCTGCATGCATTAAGTTATCACATGCGTAACTATTTCTGGTTAGACTTTGAGCAGTTGAACAACATATATCGATACAAGACTGAGGAATATTCTCACACTGCTGTAAATAAATTCAATGTTATTCCTGATTCCATTACAGATTGGGTATTTGATTTCATGCCAATTAGAGGAGGTTATTTTATTGGAAATGTCAGCCCAGCTCGGATGGACTTTAGGTGGTTTTGTTTGGGTAATTGTATTGCAATTTTGTCATCCTTGGCAACACCTGAACAGTCTACAAGAATAATGGATCTTATTGAAGAGCGGTGGGAAGATCTGGTTGGAGAGATGCCTCTCAAGGTTTCATACCCTGCCTTAGAGGGTCATGAATGGCGAATAGTAACAGGTTGCGATCCAAAGAACACAAGATGGAGTTACCACAATGGAGGATCTTGGCCAGTGCTTTTGTGGCTATTCACTGCAGCCTGTATTAAAACGGGAAGGCCGCAAATGGCCAGAAGGGCCATTGAACTTGCTGAGACCAGACTATCTAAGGATGGCTGGCCTGAATATTATGATGGGAAGCTTGGACGATATATTGGAAAGCAGTCTCGAAAGTGGCAAACCTGGTCAGTTGCTGGATATCTTGTAGCCAAGATGATGCTTGAAGATCCATCCCATTTAGGTATGATAGCATTGGAAGAGGACAAGAAGATGAAGCCGTCCCTCACTCGATCAGCTTCTTGGATAATGTAA___AATGGGGAAATCCGCCACTCTTGAATGTTTCGTCACTTCTGTATGACAAATGAGGCAATTCATAGCACATGTTGTGCAAGCATTCTCTAGCATTTTCATTCTGGTTGGTCTTAGTCGGGCCAAACTAGAAACTGCAAATAGCTCCTCCTATAATCAGATCAACTGGACAAAGCACAGTATCTTATGATGCGTCAGTCTCGTGGTATTTCAGCTCGTGTCTCATTTTCATTTCAAATAATCCCATATTCCAAGTTTTTCTGGAGTGTGTAATGGAACCAAGCAAATGTAAATGTGACTGTAATCAGTTTAATCGCT

Fln Alignment:

Contig4818___NRFSSAQNTPRSARASSEPNPMIADAWEALRRSLVYFRGKPVGTIAALDPTEDSLNYNQVFVRDFFPSALAFLMNGEPEIVKHFLLKTLRLQAWWEKRVDCFTLGEGVMPASFKVLHDPVRNSDTMIADFGESAIGRVAPVDSGFWWIILLRAYTKSTGDHSLADMPDCQRGMRLILTLCLSEGFDTFPTLLCADGCCMVDRRMGIYGYPIEIQALFFMALRCARILLKPEGGGKEFIERIDKRLHALSYHMRNYFWLDFEQLNNIYRYKTEEYSHTAVNKFNVIPDSITDWVFDFMPIRGGYFIGNVSPARMDFRWFCLGNCIAILSSLATPEQSTRIMDLIEERWEDLVGEMPLKVSYPALEGHEWRIVTGCDPKNTRWSYHNGGSWPVLLWLFTAACIKTGRPQMARRAIELAETRLSKDGWPEYYDGKLGRYIGKQSRKWQTWSVAGYLVAKMMLEDPSHLGMIALEEDKKMKPSLTRSASW
D8RY26______________NFFRSASNTPRSLL---DPNPAMIDAWEQLRRSLVYFRDKPIGTIAANDPVEESLNYNQVFVRDFVPSALAFIMNGEPEIAKNFLMKTLRLQAW-EKRIDCFTLGEGVMPASFKVLHDPSR-TDTMIADFGESAIGRVAPVDSGFWWIILLRAYVKATGDHNLSDDPDCQRGIRLILTLCLSEGFDTFPTLLCADGCCMIDRRMGIYGYPIEIQALFFMALRCAKFLLKPEAGGKEFIERIDKRLHALRYHLRSYFWLDFQQLNNIYRYKTEEYSHTAVNKFNVIPDSIPDWVFDFMPMKGGYFIGNVSPARMDFRWFTIGNCVAILSSLTTPEQATAIMDLIEERWDDLIGEMPLKVTYPALEGHEWKIVTGCDPKNTRWSYHNGGSWPVLLWLLTAACIKTGRPQIARRAIEIAEARLSKDGWPEYYDGKTGRYIGKQARKLQTWSIAGYLVAKMMLEDPSHLGMVSLEEDKKTKPYLTRSNSW

SNPs (tot: 47)

Position	A %	C %	G %	T %	Probability
104	50		50		1.0
145		25		75	0.996473
146		25		75	0.996473
147		25		75	0.996473
148	25		75		0.996473
466		70		30	1.0
612	10	90			0.90298
614		10	90		0.90298
617		90		10	0.90298
618	90	10			0.90298
619	88			11	0.923564
621	88			11	0.923564
623		88		11	0.923564
624		88		11	0.923564
625			11	88	0.923564
627		88		11	0.923564
628	88			11	0.923564
631		11	88		0.923564
633			88	11	0.923564
636	88			11	0.923564
637	88			11	0.923564
640		88		11	0.923564
641		88		11	0.923564
642	88			11	0.923564
647		11	88		0.923564
648		11		88	0.923564
649		11	88		0.923564
653		11		88	0.923564
654		11		88	0.923564
749	12			87	0.992554
750	12	87			0.992554
953	12		87		0.992554
1015			85	14	0.993533
1016	85			14	0.993533
1140	16	83			0.994512
1141	16			83	0.994512
1144	83			16	0.994512
1185		85		14	0.993533
1325	40		60		1.0
1328		60		40	1.0
1329			60	40	1.0
1381		66	33		0.997452
1477	75		25		0.996473
1621		20		80	0.995493
1867	14	85			0.993533
1869	14			85	0.993533
1955	33		66		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain