SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene3619

Length: 2100 nt

Origin of reads: Inia v1 Biogeco1 v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene3619
CGTCGTGACCTTGGCTGTCACTCAATTTTCACGCAGCAATGAGTCTCGAGATCGATCCCGGTAGCGGATATTGCAATTCCAACTGCACGTACTACAGCAAGCGAAAACCAGTAAATTTTCCCCAAGACGAATACCTGGATATCCCCACCTTCATCTCTTCATGTCCCCACAGTGGCCGAATTGCTCTCATTGATTCTGCTACAGGGGAAACCGTCACTTATCCCCATTTATGGCGGAGCGTGAGATCTCTCGCAGCCGGTTTAGCCGGCCTCGGGATCAGAAAATCTGACGTCATCCTCCTCCTCTCGCCCAATTCAATCCATTTCCCCATAATATGTTTAGCGATCATGTCCATCGGAGCCGTCGTTACAACCATTAATCCGCTAAATACGGCGGCCGAGATATCGAAGCAGGCGACAGATTCCGCCGCCGTTTTGGCCCTCGTCACGCCATCTTTGGTACACAAATTGGCGGCCACCCAGTTGCCGATGGTTCTCATTTCCTCTTCTTCTGAATTGCCCTCGCAAAATGGCCCAGATTGCATCTCTACTCTGCAGAAGCTCATGCTTTCGAATCCTGCCGACGCCCCTTCTGTGAGAATCAGGCAAGACGATACCGCCACTCTGCTTTACTCTTCCGGGACTACTGGAACCAGCAAGGGGGTCATCTCCACGCACAGAAATCTCATTTCTCTGGTTTGTATATCTCTGGAGCGCTTGAGATTGCGTTGTGAATATTGTGGAACACAGAATGAAGAAAACTCATACCTTTGCACGGTACCCATGTTTCATATATATGGGCTCTCGGCATTTGCTTGTGGGTTGCTTGGAGCCGGAAGCACCGTTGTTGTTCTGTCTAAGTTTGATCCGAGGGCGATGCTTATGTCTGTTCAGAAGTACAGAGTAACCCATTTACCCCTTGTGCCTCCCATTCTATTGGCTCTGATCAAAACAGATATTTCAAGTAAATATGATTTGAGTTCAATCAATACTGTTATATGTGGTGGAGCTACCCTGAGCAAAGAGTGCGAGGAGGAATTTGAAGCTCGGTTCCCCACAGTCACGCTATTTAAGGGCTTTGCGATGACGGAGACCACGGGAATAGGAGCATCTGCAGACAGCGAAGAAGAAAGTAGGCACCATGGATCAGTGGGCATGTTGGCTCCAAATACTGAAGCAAAAATAGTTGATCCTGAGTCTGGAGCTCCTCTACCTCCAAACAAAAGAGGAGAATTGTGGCTTCGTGGCCAATTTGTTATGAAAGGGTATTTCAACAATCCAGAAGCTACGGCTTCAGCTCTGGACGTGCATGGTTGGCTTCGAACAGGCGATCTATGTTACATTGACGATGAAGGCTACCTCTTTGTAGTGGACAGATTGAAGGAGTTGATCAAATATAAAGGCTACCAGGTTGCTCCAGCTGAACTAGAAGCACTTCTACTTTCTCACCCTGAGATTGCCGAGGCTGCAGTAATACCGTTTCCAGACAAAGAAGCTGGCCAGGTTCCCATGGCATACATTGTACAAAAACCAGGCAAAACACTATCCGAAACCAGTGTTATTGACTTTGTAGCTCAACAGGTATTTTGTATTGTAAATTCTGTATATCTATATTATCAATAGATTTTTAAAGGATTGCAAGCTAGCAGAGGAGTAGGCTTGAATTTTCTCTCTTCAAACATCTTAAACTCTGTAGCAAGTATGAGAATTGAGTGGCAAAAGCTCCTGATTCCTCTCGGTTATGGACTTCCTTTACAGGTAGCACCATACAAGAAAGTTCGCCGCGTAGCTTTTGTGAGTAAGATTCCAAAAACTGCAGCAGGAAAGATATTAAGGAAGGACTTAATTAAGTTGTCCACATCCAAACTTTGATGCAATTGTGGAATTATAAATTTCAATTGCCTGTTAGAAAATAAGTCTGCATCACAAATTTGTCTGAGCAATCATGGAAAATAAGTAAAAATCCATTATCTGCAGCATCAGAGTTACTGAAGCCACATCAACTGCACGAGAATTCCTAAGTAGAGGCACTCTGCGGTTTAACGAATATGCTATATGTACGATGTCATGTACCCAAATAATTAGATCATCGAATAAGAAA

Ace file of the UniGene sp_v2.0_unigene3619 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	AMP dependent CoA ligase, putative n=1 Tax=Ricinus communis RepID=B9R8J7_RICCO	-	-	49%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	68%
Blast2go	acyl:coa ligase	-	-	69%

Source	ECs	Term	Type	e value	Identity
Blast2go	4-coumarate--CoA ligase.	EC:6.2.1.12	-	0.0	69%

Source	KEGGs	Term	Identity
Blast2go	Ubiquinone and other terpenoid-quinone biosynthesis	00130	69%
Blast2go	Phenylalanine metabolism	00360	69%
Blast2go	Phenylpropanoid biosynthesis	00940	69%
Blast2go	Biosynthesis of phenylpropanoids	01061	69%
Blast2go	Biosynthesis of alkaloids derived from shikimate pathway	01063	69%
Blast2go	Metabolic pathways	01100	69%
Blast2go	Biosynthesis of secondary metabolites	01110	69%

Source	GOs	Term	Type	Identity
Blast2go	jasmonic acid biosynthetic process	GO:0009695	Biological Process	69%
Blast2go	response to wounding	GO:0009611	Biological Process	69%
Blast2go	phenylpropanoid metabolic process	GO:0009698	Biological Process	69%
Blast2go	4-coumarate-CoA ligase activity	GO:0016207	Molecular Function	69%
Blast2go	peroxisome	GO:0005777	Cellular Component	69%

Source	InterPros	Term	Type	Identity
Sma3	Regulator of chromosome condensation, RCC1	IPR000408	-	-
Sma3	AMP-dependent synthetase/ligase	IPR000873	-	-
Sma3	Adenosine/AMP deaminase active site	IPR006650	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5ABX8

Fln msg: your sequence is longer than subject: 611 - 373

Fln protein: MSLEIDPGSGYCNSNCTYYSKRKPVNFPQDEYLDIPTFISSCPHSGRIALIDSATGETVTYPHLWRSVRSLAAGLAGLGIRKSDVILLLSPNSIHFPIICLAIMSIGAVVTTINPLNTAAEISKQATDSAAVLALVTPSLVHKLAATQLPMVLISSSSELPSQNGPDCISTLQKLMLSNPADAPSVRIRQDDTATLLYSSGTTGTSKGVISTHRNLISLVCISLERLRLRCEYCGTQNEENSYLCTVPMFHIYGLSAFACGLLGAGSTVVVLSKFDPRAMLMSVQKYRVTHLPLVPPILLALIKTDISSKYDLSSINTVICGGATLSKECEEEFEARFPTVTLFKGFAMTETTGIGASADSEEESRHHGSVGMLAPNTEAKIVDPESGAPLPPNKRGELWLRGQFVMKGYFNNPEATASALDVHGWLRTGDLCYIDDEGYLFVVDRLKELIKYKGYQVAPAELEALLLSHPEIAEAAVIPFPDKEAGQVPMAYIVQKPGKTLSETSVIDFVAQQVFCIVNSVYLYYQ*IFKGLQASRGVGLNFLSSNILNSVASMRIEWQKLLIPLGYGLPLQVAPYKKVRRVAFVSKIPKTAAGKILRKDLIKLSTSKL*

Protein Length: 612

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTTTCACGCAGCA_-_ATGAGTCTCGAGATCGATCCCGGTAGCGGATATTGCAATTCCAACTGCACGTACTACAGCAAGCGAAAACCAGTAAATTTTCCCCAAGACGAATACCTGGATATCCCCACCTTCATCTCTTCATGTCCCCACAGTGGCCGAATTGCTCTCATTGATTCTGCTACAGGGGAAACCGTCACTTATCCCCATTTATGGCGGAGCGTGAGATCTCTCGCAGCCGGTTTAGCCGGCCTCGGGATCAGAAAATCTGACGTCATCCTCCTCCTCTCGCCCAATTCAATCCATTTCCCCATAATATGTTTAGCGATCATGTCCATCGGAGCCGTCGTTACAACCATTAATCCGCTAAATACGGCGGCCGAGATATCGAAGCAGGCGACAGATTCCGCCGCCGTTTTGGCCCTCGTCACGCCATCTTTGGTACACAAATTGGCGGCCACCCAGTTGCCGATGGTTCTCATTTCCTCTTCTTCTGAATTGCCCTCGCAAAATGGCCCAGATTGCATCTCTACTCTGCAGAAGCTCATGCTTTCGAATCCTGCCGACGCCCCTTCTGTGAGAATCAGGCAAGACGATACCGCCACTCTGCTTTACTCTTCCGGGACTACTGGAACCAGCAAGGGGGTCATCTCCACGCACAGAAATCTCATTTCTCTGGTTTGTATATCTCTGGAGCGCTTGAGATTGCGTTGTGAATATTGTGGAACACAGAATGAAGAAAACTCATACCTTTGCACGGTACCCATGTTTCATATATATGGGCTCTCGGCATTTGCTTGTGGGTTGCTTGGAGCCGGAAGCACCGTTGTTGTTCTGTCTAAGTTTGATCCGAGGGCGATGCTTATGTCTGTTCAGAAGTACAGAGTAACCCATTTACCCCTTGTGCCTCCCATTCTATTGGCTCTGATCAAAACAGATATTTCAAGTAAATATGATTTGAGTTCAATCAATACTGTTATATGTGGTGGAGCTACCCTGAGCAAAGAGTGCGAGGAGGAATTTGAAGCTCGGTTCCCCACAGTCACGCTATTTAAGGGCTTTGCGATGACGGAGACCACGGGAATAGGAGCATCTGCAGACAGCGAAGAAGAAAGTAGGCACCATGGATCAGTGGGCATGTTGGCTCCAAATACTGAAGCAAAAATAGTTGATCCTGAGTCTGGAGCTCCTCTACCTCCAAACAAAAGAGGAGAATTGTGGCTTCGTGGCCAATTTGTTATGAAAGGGTATTTCAACAATCCAGAAGCTACGGCTTCAGCTCTGGACGTGCATGGTTGGCTTCGAACAGGCGATCTATGTTACATTGACGATGAAGGCTACCTCTTTGTAGTGGACAGATTGAAGGAGTTGATCAAATATAAAGGCTACCAGGTTGCTCCAGCTGAACTAGAAGCACTTCTACTTTCTCACCCTGAGATTGCCGAGGCTGCAGTAATACCGTTTCCAGACAAAGAAGCTGGCCAGGTTCCCATGGCATACATTGTACAAAAACCAGGCAAAACACTATCCGAAACCAGTGTTATTGACTTTGTAGCTCAACAGGTATTTTGTATTGTAAATTCTGTATATCTATATTATCAATAGATTTTTAAAGGATTGCAAGCTAGCAGAGGAGTAGGCTTGAATTTTCTCTCTTCAAACATCTTAAACTCTGTAGCAAGTATGAGAATTGAGTGGCAAAAGCTCCTGATTCCTCTCGGTTATGGACTTCCTTTACAGGTAGCACCATACAAGAAAGTTCGCCGCGTAGCTTTTGTGAGTAAGATTCCAAAAACTGCAGCAGGAAAGATATTAAGGAAGGACTTAATTAAGTTGTCCACATCCAAACTTTGA___TGCAATTGTGGAATTATAAATTTCAATTGCCTGTTAGAAAATAAGTCTGCATCACAAATTTGTCTGAGCAATCATGGAAAATAAGTAAAAATCCATTATCTGCAGCATCAGAGTTACTGAAGCCACATCAACTGCACGAGAATTCCTAAGTAGAGGCACTCTGCGGTTTAACGAATATGCTATATGTACGATGTCATGTACCCAAATAATTAGATCATCGAATAAGAAA

Fln Alignment:

Contig3619___MLSNPADAPSVRIRQDDTATLLYXXXXXXXXXGVISTHRNLISLVCISLERLRLRCEYCGTQNEENSYLCTVPMFHIYGLSAFACGLLGAGSTVVVLSKFDPRAMLMSVQKYRVTHXXXXXXXXXXXIKTDISSKYDLSSINTVICGGATLSKECEEEFEARFPTVTLFKGFAMTETTGIGASADSEEESRHHGSVGMLAPNTEAKIVDPESGAPLPPNKRGELWLRGQFVMKGYFNNPEATASALDVHGWLRTGDLCYIDDEGYLFVVDRLKELIKYKGYQVAPAELEALLLSHPEIAEAAVIPFPDKEAGQVPMAYIVQKPGKTLSETSVIDFVAQQVFCIVNSVYLYYQ*IFKGLQASRGVGLNFLSSNILNSVASMRIEWQKLLIPLGYGLPLQVAPYKKVRRVAFVSKIPKTAAGKILRKDLIKLSTSKL
D5ABX8______________MASNPADAPSVKIRQDDTATLLYSSGTTGTSKGVISTHRNLISVLCIFLSRLRLNREYL--------YLCTVPMFHVYGLSAFACGLLGSGSTIVVLSKFDVMEMLAAVEKYRVTYLPIVPPILLALTKTDIARKYDLRSLHTVICGGAPLSKESAEEFVARFPSVSLLQGYGLTETTGRGASTENEEESRHYGSVGMLTPNIEAKIVDPDSITPLPPNKKGELWLRGPVVMKGYFNNPEATASALDKDGWLRTGDLCYIDDNGYLFVVDRLKELIKYKGYQVAPAELEALLLSHPEIAEAAVIPFEDREAGQVPMAYIVRKP-----------------------------------------GSTLYETNVTDFVAQ------------------QVAPYKKVRRVAFVSEIPKTAAGKTLRKDLIKLATSKL

SNPs (tot: 17)

Position	A %	C %	G %	T %	Probability
1128	85		14		0.993533
1297	11		88		0.923564
1301		11	88		0.923564
1302	12	87			0.992554
1303		87		12	0.992554
1304		87		12	0.992554
1397	88		11		0.923564
1640	20		80		0.995493
1753	90		10		0.90298
1767			10	90	0.90298
1768	90	10			0.90298
1769			10	90	0.90298
1770	10			90	0.90298
1875	80		20		0.995493
1914		14	85		0.993533
1916			85	14	0.993533
1917	85			14	0.993533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain