SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene2740

Length: 2474 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene2740
CATTAACTTTTCTATGGGGTACTGCCGAGTATATTATTTGGGGTGGTCCATAGTGCCACCTGGCTTTGACTGCGTTTCTTACAAAATTTTCAGCGTTTGAAGGCAGGCAGTCTTCTAGAAACACGACCGGAGATCCTTCCTCTTTGAATCCACGGTGGAATCATTCTCATCATTTTCTATTCGAAGGTCCCGGATCAAAGCCGGCGCCGCGATTGGGCTCTGGCCTAAAATTTTAACTTTCCGACTTGTTACTATATCCGAGAACAGCCCCGGGGAAAAATTAGCTTGCATCTATCATAAATGCAGGTGTGTGCCTTTTGCAAAGGCTGACATCTAACTCGGAGAGAGCGAGCCATGGCTGGAATGAAGCAGAGGAAAAGAACTAAAACGATCTCGGCCGTGGCGCCTAATGACGATCACGTGCGGAGTTCCGGCTGCTTTTATTGTGCTCGTGGTTCTCCCATTCACTCCCCGGCCGAAAATCCACGCGAAAATGGAATCAGTATGGCTCCCGTTGTCTCAGAGAGCAGAAATGGCGGACAGGACGTGTGGGATGCCGCTGAAGTCGAACGCCGGGAGGCACAATCCGGCGCCGCGAAGGCATTTCCTGAACATCTCGTAATCATGGTGAACGGCATCATTGGCAATGCAACCAATTGGCAGTATGCTGCTGATCAATTTGTAAAGAGACTACCCGACAAAGTTGTTGTCCACTGCAGTCAATGCAATTCTGCAACAAAGACTTTTGATGGAGTTGATCAAATGGGCAATCGACTAGCAGAAGAGGTAAAGAAAGTCATAGAAAACAGGCCTGGAGTAAAACGAATTTCATTTGTGAGTCACTCTTTAGGTGGCTTGGTATCAAGGTATGCTATTGGAGTACTATACGAGCCACCTTCAGGAAGTAGCACTGTAGCACAGAATAATTGTGATATAATTGATGGCAACTTCAATCAAAATGTTGAAGAAGAGTCAAAGGGTAAAATTGCTGGACTAGAGCCTGTGAATTTCATAACTTTTGCAACACCTCACTTGGGCTCTAGGGGATATAAGCAGCTTCCATTTGTTTGTGGTCTTCCCGTGCTGGAGAAAGCTGCATCTCGTAGCGCACACTGGTTTGTTGGGAGGACTGGTCGGCACCTTTTCCTTACTGACAAGGATGATGGGAAGCCTCCACTTCTGCGTCGATTGGTGACTGATTTTGGTGACCTGTATTTTATGTCTGCTTTGCGGGCATTCAAGCGGCGTGTTGCGTATTCAAATGTGAGCTATGACCATATGGTTGGCTGGCGGACTGCTTCCATTCGGCGCCAGTGTGAATTGCCTAAGCTGCAGAAGGTTCCTGTAAATGAGAAATATCCACACATTGTTAATGTAGAAATGGAAATGATGGTAATTCTATTCCAAGAGTAGCTGCCTCAAAAAGTGACCATATTACTGATGCTGCAGAAGAGGAAATGGTTGCAGCTCTTACCCAACTACCATGGGAAAGAGTTGATGTCAGCTTCCATAAAACCAAGCAACGATTGTTTGCTCATAGCACAATACAGGTAAAATGGTACTGGATGCATTCGGAAGGTTCTGACGTGATATCTCATTTGATTGATAATTTTGTTGTCCAATAGGAGGGTAATGTCAGTTACCAATAGAACAATCTTAAAAATATAATGCTAATTCTATGTAGCTTTATCTAGCTCATGGAAGGCTCTAAAGCATCTTTATCTGCCTTCCATGGTAGAATGTCATTAGCTTTGGGATGTCCACTTCATAATAGATCATACAAAAACCATGTTTATGAATCCTTTCTTCCATGGGTAGAAGGTATGTGATGTCAGAAAGAGGTCATTGATTCCTTCCCTGAGACTATTGCCACGCTGTTGTGGCTGGTTCTCAAGGTCTTGTATTAATCTGTTTATTTCTGCATTATTTGCTTTTGTTGCACAAAAGCTGAAATTCAATCGTTTCTGGAGGTGATTTGTCAATGGTCCAGTGTTCTAATCTCCTCGTGTTACTGGCAAATTAAAGTCCTCAATGGATATGATTGCCATTAACATTTTTGTACGTCATTGTACTTGTAATGAATTTTGGGTTGTTAGCCAGTCACCTGTACATCATGTATAGAAAGCAAGATGCATGAACATAGGTGATATGATTTATACTCTACTTCCAACGACATAGATGATATTGATGGATACTCCAAACGAGGTTCCAAAACTTGGGATATGCAGAAGATAAGCAGGATGGCATATTTTGGAGTGAAGTTGATAACCATGTTGTACTTTGTGCACAATCAATTGTTGCATGCAAAGGAAAGGCAAGACATCCTGAACAAATGGTAGTTGCAAGCAGAGGCTCCTCATTGATTTGACATTTTCCTTTGTCAGTCCCTGATTGTTTATAAACTTTTGTTAAAATAAGGGAAGATGTTTTGTTAAACTAGTAGGTCAACTTATAATATGAAGGATGCATAACTC

Ace file of the UniGene sp_v2.0_unigene2740 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	DUF676 domain containing protein	-	-	42%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	44%
Blast2go	serine esterase family protein	-	-	74%

Source	GOs	Term	Type	e value	Identity
Blast2go	mitochondrion	GO:0005739	Cellular Component	0.0	74%

Source	InterPros	Term	Type	Identity
Sma3	Ribosomal protein S8	IPR000630	-	-
Sma3	Short-chain dehydrogenase/reductase SDR	IPR002198	-	-
Sma3	Domain of unknown function DUF676, lipase-like	IPR007751	-	-
Sma3	SGT1	IPR010770	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LL85

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 96 aas, putative chimeric sequence, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 353 - 464

Fln protein: MAGMKQRKRTKTISAVAPNDDHVRSSGCFYCARGSPIHSPAENPRENGISMAPVVSESRNGGQDVWDAAEVERREAQSGAAKAFPEHLVIMVNGIIGNATNWQYAADQFVKRLPDKVVVHCSQCNSATKTFDGVDQMGNRLAEEVKKVIENRPGVKRISFVSHSLGGLVSRYAIGVLYEPPSGSSTVAQNNCDIIDGNFNQNVEEESKGKIAGLEPVNFITFATPHLGSRGYKQLPFVCGLPVLEKAASRSAHWFVGRTGRHLFLTDKDDGKPPLLRRLVTDFGDLYFMSALRAFKRRVAYSNVSYDHMVGWRTASIRRQCELPKLQKVPVNEKYPHIVNVEMEMMVILFQE*

Protein Length: 354

Fln nts: CATTAACTTTTCTATGGGGTACTGCCGAGTATATTATTTGGGGTGGTCCATAGTGCCACCTGGCTTTGACTGCGTTTCTTACAAAATTTTCAGCGTTTGAAGGCAGGCAGTCTTCTAGAAACACGACCGGAGATCCTTCCTCTTTGAATCCACGGTGGAATCATTCTCATCATTTTCTATTCGAAGGTCCCGGATCAAAGCCGGCGCCGCGATTGGGCTCTGGCCTAAAATTTTAACTTTCCGACTTGTTACTATATCCGAGAACAGCCCCGGGGAAAAATTAGCTTGCATCTATCATAAATGCAGGTGTGTGCCTTTTGCAAAGGCTGACATCTAACTCGGAGAGAGCGAGCC_-_ATGGCTGGAATGAAGCAGAGGAAAAGAACTAAAACGATCTCGGCCGTGGCGCCTAATGACGATCACGTGCGGAGTTCCGGCTGCTTTTATTGTGCTCGTGGTTCTCCCATTCACTCCCCGGCCGAAAATCCACGCGAAAATGGAATCAGTATGGCTCCCGTTGTCTCAGAGAGCAGAAATGGCGGACAGGACGTGTGGGATGCCGCTGAAGTCGAACGCCGGGAGGCACAATCCGGCGCCGCGAAGGCATTTCCTGAACATCTCGTAATCATGGTGAACGGCATCATTGGCAATGCAACCAATTGGCAGTATGCTGCTGATCAATTTGTAAAGAGACTACCCGACAAAGTTGTTGTCCACTGCAGTCAATGCAATTCTGCAACAAAGACTTTTGATGGAGTTGATCAAATGGGCAATCGACTAGCAGAAGAGGTAAAGAAAGTCATAGAAAACAGGCCTGGAGTAAAACGAATTTCATTTGTGAGTCACTCTTTAGGTGGCTTGGTATCAAGGTATGCTATTGGAGTACTATACGAGCCACCTTCAGGAAGTAGCACTGTAGCACAGAATAATTGTGATATAATTGATGGCAACTTCAATCAAAATGTTGAAGAAGAGTCAAAGGGTAAAATTGCTGGACTAGAGCCTGTGAATTTCATAACTTTTGCAACACCTCACTTGGGCTCTAGGGGATATAAGCAGCTTCCATTTGTTTGTGGTCTTCCCGTGCTGGAGAAAGCTGCATCTCGTAGCGCACACTGGTTTGTTGGGAGGACTGGTCGGCACCTTTTCCTTACTGACAAGGATGATGGGAAGCCTCCACTTCTGCGTCGATTGGTGACTGATTTTGGTGACCTGTATTTTATGTCTGCTTTGCGGGCATTCAAGCGGCGTGTTGCGTATTCAAATGTGAGCTATGACCATATGGTTGGCTGGCGGACTGCTTCCATTCGGCGCCAGTGTGAATTGCCTAAGCTGCAGAAGGTTCCTGTAAATGAGAAATATCCACACATTGTTAATGTAGAAATGGAAATGATGGTAATTCTATTCCAAGAGTAGCTGCCTCAAAAAGTGACCATATTACTGATGCTGCAGAAGAGGAAATGGTTGCAGCTCTTACCCAACTACCATGGGAAAGAGTTGATGTCAGCTTCCATAAAACCAAGCAACGATTGTTTGCTCATAGCACAATACAGGTAAAATGGTACTGGATGCATTCGGAAGGTTCTGACGTGATATCTCATTTGATTGATAATTTTGTTGTCCAATAGGAGGGTAATGTCAGTTACCAATAGAACAATCTTAAAAATATAATGCTAATTCTATGTAGCTTTATCTAGCTCATGGAAGGCTCTAAAGCATCTTTATCTGCCTTCCATGGTAGAATGTCATTAGCTTTGGGATGTCCACTTCATAATAGATCATACAAAAACCATGTTTATGAATCCTTTCTTCCATGGGTAGAAGGTATGTGATGTCAGAAAGAGGTCATTGATTCCTTCCCTGAGACTATTGCCACGCTGTTGTGGCTGGTTCTCAAGGTCTTGTATTAATCTGTTTATTTCTGCATTATTTGCTTTTGTTGCACAAAAGCTGAAATTCAATCGTTTCTGGAGGTGATTTGTCAATGGTCCAGTGTTCTAATCTCCTCGTGTTACTGGCAAATTAAAGTCCTCAATGGATATGATTGCCATTAACATTTTTGTACGTCATTGTACTTGTAATGAATTTTGGGTTGTTAGCCAGTCACCTGTACATCATGTATAGAAAGCAAGATGCATGAACATAGGTGATATGATTTATACTCTACTTCCAACGACATAGATGATATTGATGGATACTCCAAACGAGGTTCCAAAACTTGGGATATGCAGAAGATAAGCAGGATGGCATATTTTGGAGTGAAGTTGATAACCATGTTGTACTTTGTGCACAATCAATTGTTGCATGCAAAGGAAAGGCAAGACATCCTGAACAAATGGTAGTTGCAAGCAGAGGCTCCTCATTGATTTGACATTTTCCTTTGTCAGTCCCTGATTGTTTATAAACTTTTGTTAAAATAAGGGAAGATGTTTTGTTAAACTAGTAGGTCAACTTATAATATGAAGGATGCATAACTC

Fln Alignment:

Contig2740___EVERREAQSGAAKAFPEHLVIMVNGIIGNATNWQYAADQFVKRLPDKVVVHCSQCNSATKTFDGVDQMGNRLAEEVKKVIENRPGVKRISFVSHSLGGLVSRYAIGVLYEPPSGS---STVAQNNCDIIDGNFNQNVEEE----SKGKIAGLEPVNFITFATPHLGSRGYKQLPFVCGLPVLEKAASRSAHWFVGRTGRHLFLTDKDDGKPPLLRRLVTDFGDLYFMSALRAFKRRVAYSNVSYDHMVGWRTASIRRQCELPKLQKVPVNEKYPHIVNV
B8LL85______________KVQMGERNGASVDITPDHLLVLVHGIMASPSDWKYVEAELKRRLGNRFFIYASAANSYTQTLHGIDIAGRRLAEEVQQVIQKTPSLKRISFLAHSLGGLFARYAIAVLYRENSDTHVQSSEPDFKVEMLEKKSVAELKDQYTKVPKATIAGLEAINFVTLATPHLGVRGKKQLPFLLGVPLLEKLAPPLAQYVIRRTGRQLFLTDGKYSKPPLLLRMASDCEDGKFISALAIFRSRILYANVSYDHMVGWRTSSIRRESELVKPPEHSL-DGYKHVVDV

SNPs (tot: 69)

Position	A %	C %	G %	T %	Probability
521	88		11		0.923564
522	11			88	0.923564
523			11	88	0.923564
524	11			88	0.923564
529		14		85	0.993533
530	14			85	0.993533
531	14			85	0.993533
532			14	85	0.993533
535		14	85		0.993533
537	85		14		0.993533
540	85		14		0.993533
589	11	88			0.923564
592		88		11	0.923564
677		16		83	0.994512
777	11			88	0.923564
809	85			14	0.993533
814	14			85	0.993533
949		33		66	1.0
1075	83			16	0.994512
1078	83		16		0.994512
1080			16	83	0.994512
1083	16	83			0.994512
1084	80		20		0.995493
1085		20		80	0.995493
1088	20			80	0.995493
1089			20	80	0.995493
1095	20		20		0.99255
1097		20		80	0.995493
1098			20	40	1.0
1100	33			66	1.0
1101	33	66			1.0
1103	66		33		1.0
1104		66		33	1.0
1142		12		87	0.992554
1143		12		87	0.992554
1146	12		87		0.992554
1247		85		14	0.993533
1375	40		60		1.0
1426		83		16	0.994512
1502		14		85	0.993533
1503		14		85	0.993533
1657	33		66		1.0
1738	25			75	0.996473
1740	25			75	0.996473
1810	16		83		0.994512
1811		83		16	0.994512
1812	83		16		0.994512
1813			83	16	0.994512
1944			50	50	1.0
2135		85	14		0.993533
2151	14			85	0.993533
2152		85	14		0.993533
2153	57	28		14	1.0
2154		28	71		1.0
2155	14	85			0.993533
2157	42			57	1.0
2158			14	85	0.993533
2159		20		80	0.995493
2164	80		20		0.995493
2167			80	20	0.995493
2174	80		20		0.995493
2236	75		25		0.996473
2281			75	25	0.996473
2290		66	33		0.997452
2337	66	33			0.997452
2338	66	33			0.997452
2339	66	33			0.997452
2340		33	66		0.997452
2342			66	33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain