SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene2739

Length: 2354 nt

Origin of reads: Biogeco1 v2 Uagpf v2 Uma v2

UniGene Fasta

>sp_v2.0_unigene2739
TCTTCAAAGCATCTCTTACTTCGCTGTAATGAGTTTTTTCTTCTTGTTTAATAAGGTTTAGTTTATGTCTCTATTGTAATCACCTGAGTAAATCTCTTCTCAGTCTTGCATCTTATAAGGCTTTTTCATTATATCAATGTTTGATGTAGGGATCTGAAAGCTGAAAATGTTTTAATGGGTGCTGATGGTGTATGGAAGTTATGTGATTTTGGTAGCACTTCTACAAATCACAAGCGATTTGAGAGGCTCGTGACCTTGGCTGTCACTCAAGAAGACATCATTAGAAAACACACAACTCCAGCTTACAGAGCACCTGAGATGTGGGATCTCTACAGAAGAGAGCTAATAAGTGAAAAGGTTGATATCGTCGGGCCCTTGGGTGTCTACTTTACAGGATAGCTTATTTAAAGTCTGCTTTTGACGGTGAATCGAAGCTACAAATATTGAATGGGAACTACCGCATTCCCGAGTTACCAAGATACAGTTCTTCATTGGTTGAATTGATCAAGGACATGCTCACAGCTGCTCCAGAAGTCAGACCCGATATCATGCAGATATGGCGCCGTGTAAATGAACTTCTACCAGTTGAGTTGCGTAAATCAAGTCCAGATAGACCATTATCTATGGCTAATTCTGAGGTACATGCTCCTCCTATGGACGCCCAAGATCATGGTAATCCTGTGCCAACAAGGCGAGCTCCTCCACTCCCATCTAGAACACCACCACCTCCACCTACATCAAAAGACCAAGATCGTCAGTCACCACCTATGGGACAACCAGATTCAAAGGCAATACAAGGTGGTGTTAGTAGTGGGAGCTCTTTAGGAGCATTTTGGTCAAGTCAGTATGCACAGGAATCTGTGCCCGTGGATGACAAAGGCCCCGTGTTTGATAAGGATCCTGGGAGTGAAAGTGTATTCAAGCAGCGGTCACAAAGCCCTGAAAGTCATCCAAAACAAGGCATTTTAAGTTCACCAAAGGAAAATCAGTCCAGAGCTGGACACATTTTGAAAAGCGGTATGTCAAACTTTATGAAGAAGGTGCATGCCTCACCACAACCTACAACACGGCAGGGATATGATGAAATGAATTCAGATATAAACCAGATAAGGCTTAGCTCTGAGGATACTGACCATGGGGATGAAAACACAATCGACTCTGGTGTATCTCCAGTGAAGGCTTCAGCTTCAGAAATTGGTGGGCCATTTCATGACGATTCATTTAATGCATTTGTTGCAGACTTTGATAACCATAAGCCCACCACTGGAACTAATGTGTTTGGCAAAGAAAGTTTACAAGTAGAAGTGGACAGATTGAAAGAAGAGCTGGAGCAGGTGCGCTTAGAAAAGGCAGAAATCACATCAAAGTATGAAAAATTGACAGCTATCTGCCGTTCTCAAAGGCAAGAGATACAAGAGTTAAAACAGACCTTGTCTACAGCAACTGCTCCGAACTTCACCACATTCAGTAAAGAAGGTAGCACAAAGCCAGTTGTACCCCAACAGGCACAAGTGCAACAGCTGAAACAACAGCAAAAACAGCGGTTGGTACCTGGAAGCTGGCACCCAGGCAACAAGCAAGAAGACAAGACAGAGGGATCTATTTGGGAACTGCAGGAAGGTATGTCTACTATGTCCTCCCATGGCGTGCCCTTCAAGGCCACTGATGCGAAGGAATGGCAACCATTTGGTGAATCACTGACCACAAAGTTGTCAAATTCTCCCCCATCCAATGTGTCTGGAGCCTCAAATGGTTACCGCCCTCAGCATCATGCAAGTGGTTCAAGTGTTGGTTCCGGTGACACTTGGGGTTTTGTGCAGGATAGTTTCACCCCAGTTCGTAATAATGTACAGGCATCTAAGTCTTCTGGTAGTGAAAGCAAGGGTAATCCAGCAGTTTATGATTCATACAGTTCAAAATCTGATGGCCGAAGTTCACGATCTATAACTCAATCTCAACCTGCAGGATGGGCTGGATTTTGAGGATCAAGAAATTAATGCACTTGGTTATCCAAAGTTAAGTGTGCTGGGAAAACATCCCATGGTATTTATGCAAGATTTTCCTGTTTGCTCGTGAGGCATAACATTGTGAAGTTTTACTAAGGTGCAAGGTTATTGACTGCAGAAAATCAAGTTAGAGTTTCTTCTTAGAGATTCAGAAGTACAAATTTTCTGACTGTATAAGTGTGGTTTCATGTTATTATATGGAAATTTCAATTTGAGGTTGCCATGTTGGTAGTCAACAGTAGAACTTGAGATTTTATTTGATGCTTTCAAAAGTCATCAGAAGTTGGTTGTCATTTTAATAACGATTATTTGAATGCCAATTATATGAGTATTTTGCCATTAGCAAAA

Ace file of the UniGene sp_v2.0_unigene2739

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	PREDICTED: similar to protein kinase family protein n=1 Tax=Vitis vinifera RepID=UPI00019849D1	-	-	36%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	33%
Blast2go	serine threonine protein kinase	-	-	51%

Source	ECs	Term	Type	e value	Identity
Blast2go	Transferases, Transferring phosphorous-containing groups, Protein-serine/threonine kinases.	EC:2.7.11.-	-	0.0	51%

Source	GOs	Term	Type	Identity
Blast2go	protein phosphorylation	GO:0006468	Biological Process	51%
Blast2go	ATP binding	GO:0005524	Molecular Function	51%
Blast2go	protein serine/threonine kinase activity	GO:0004674	Molecular Function	51%

Source	InterPros	Term	Type	Identity
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3		IPR017442	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5A7T1

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 74 aas, putative chimeric sequence, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 75 - 197

Fln protein: MGADGVWKLCDFGSTSTNHKRFERLVTLAVTQEDIIRKHTTPAYRAPEMWDLYRRELISEKVDIVGPLGVYFTG*

Protein Length: 76

Fln nts: TCTTCAAAGCATCTCTTACTTCGCTGTAATGAGTTTTTTCTTCTTGTTTAATAAGGTTTAGTTTATGTCTCTATTGTAATCACCTGAGTAAATCTCTTCTCAGTCTTGCATCTTATAAGGCTTTTTCATTATATCAATGTTTGATGTAGGGATCTGAAAGCTGAAAATGTTTTA_-_ATGGGTGCTGATGGTGTATGGAAGTTATGTGATTTTGGTAGCACTTCTACAAATCACAAGCGATTTGAGAGGCTCGTGACCTTGGCTGTCACTCAAGAAGACATCATTAGAAAACACACAACTCCAGCTTACAGAGCACCTGAGATGTGGGATCTCTACAGAAGAGAGCTAATAAGTGAAAAGGTTGATATCGTCGGGCCCTTGGGTGTCTACTTTACAGGATAGCTTATTTAAAGTCTGCTTTTGACGGTGAATCGAAGCTACAAATATTGAATGGGAACTACCGCATTCCCGAGTTACCAAGATACAGTTCTTCATTGGTTGAATTGATCAAGGACATGCTCACAGCTGCTCCAGAAGTCAGACCCGATATCATGCAGATATGGCGCCGTGTAAATGAACTTCTACCAGTTGAGTTGCGTAAATCAAGTCCAGATAGACCATTATCTATGGCTAATTCTGAGGTACATGCTCCTCCTATGGACGCCCAAGATCATGGTAATCCTGTGCCAACAAGGCGAGCTCCTCCACTCCCATCTAGAACACCACCACCTCCACCTACATCAAAAGACCAAGATCGTCAGTCACCACCTATGGGACAACCAGATTCAAAGGCAATACAAGGTGGTGTTAGTAGTGGGAGCTCTTTAGGAGCATTTTGGTCAAGTCAGTATGCACAGGAATCTGTGCCCGTGGATGACAAAGGCCCCGTGTTTGATAAGGATCCTGGGAGTGAAAGTGTATTCAAGCAGCGGTCACAAAGCCCTGAAAGTCATCCAAAACAAGGCATTTTAAGTTCACCAAAGGAAAATCAGTCCAGAGCTGGACACATTTTGAAAAGCGGTATGTCAAACTTTATGAAGAAGGTGCATGCCTCACCACAACCTACAACACGGCAGGGATATGATGAAATGAATTCAGATATAAACCAGATAAGGCTTAGCTCTGAGGATACTGACCATGGGGATGAAAACACAATCGACTCTGGTGTATCTCCAGTGAAGGCTTCAGCTTCAGAAATTGGTGGGCCATTTCATGACGATTCATTTAATGCATTTGTTGCAGACTTTGATAACCATAAGCCCACCACTGGAACTAATGTGTTTGGCAAAGAAAGTTTACAAGTAGAAGTGGACAGATTGAAAGAAGAGCTGGAGCAGGTGCGCTTAGAAAAGGCAGAAATCACATCAAAGTATGAAAAATTGACAGCTATCTGCCGTTCTCAAAGGCAAGAGATACAAGAGTTAAAACAGACCTTGTCTACAGCAACTGCTCCGAACTTCACCACATTCAGTAAAGAAGGTAGCACAAAGCCAGTTGTACCCCAACAGGCACAAGTGCAACAGCTGAAACAACAGCAAAAACAGCGGTTGGTACCTGGAAGCTGGCACCCAGGCAACAAGCAAGAAGACAAGACAGAGGGATCTATTTGGGAACTGCAGGAAGGTATGTCTACTATGTCCTCCCATGGCGTGCCCTTCAAGGCCACTGATGCGAAGGAATGGCAACCATTTGGTGAATCACTGACCACAAAGTTGTCAAATTCTCCCCCATCCAATGTGTCTGGAGCCTCAAATGGTTACCGCCCTCAGCATCATGCAAGTGGTTCAAGTGTTGGTTCCGGTGACACTTGGGGTTTTGTGCAGGATAGTTTCACCCCAGTTCGTAATAATGTACAGGCATCTAAGTCTTCTGGTAGTGAAAGCAAGGGTAATCCAGCAGTTTATGATTCATACAGTTCAAAATCTGATGGCCGAAGTTCACGATCTATAACTCAATCTCAACCTGCAGGATGGGCTGGATTTTGAGGATCAAGAAATTAATGCACTTGGTTATCCAAAGTTAAGTGTGCTGGGAAAACATCCCATGGTATTTATGCAAGATTTTCCTGTTTGCTCGTGAGGCATAACATTGTGAAGTTTTACTAAGGTGCAAGGTTATTGACTGCAGAAAATCAAGTTAGAGTTTCTTCTTAGAGATTCAGAAGTACAAATTTTCTGACTGTATAAGTGTGGTTTCATGTTATTATATGGAAATTTCAATTTGAGGTTGCCATGTTGGTAGTCAACAGTAGAACTTGAGATTTTATTTGATGCTTTCAAAAGTCATCAGAAGTTGGTTGTCATTTTAATAACGATTATTTGAATGCCAATTATATGAGTATTTTGCCATTAGCAAAA

Fln Alignment:

Contig2739___DLKAENVLMGADGVWK--------LCDFGSTSTNHKRFERLVTLAVTQEDIIRKHTTPAYRAPEMWDLYRRELISEKVDI
D5A7T1______________DVKPGNVLI----TWRKSQPPLAVLMDFGSAAPA-KREVRSRSQALQVQEWAAEHCSAPYRAPELWDCPSNSDIDERTDI

SNPs (tot: 14)

Position	A %	C %	G %	T %	Probability
1280		20		80	0.995493
1651	14		85		0.993533
1662	14	85			0.993533
1751	20		20		0.99255
1800	75		25		0.996473
1841		25		75	0.996473
1876			88	11	0.923564
1877			11	88	0.923564
1879	88		11		0.923564
1885	77		22		0.999999
2022	87		12		0.992554
2029		87		12	0.992554
2314	72	27			1.0
2331	80		20		0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain