SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene2645

Length: 2072 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene2645
CGTCGTGACCTTGGCTGTCACTCAACAATCCACGATAGAAAGGAGATCGAGAGCTTATCTCAGCCACAAAACAAAAGAAAGAGTATTGTTTACCAAAAATAAGAAGTTGCAGCAGGACCATCCCGTCCCATAATTCCCAATTAATTCTCCGCAAATCCTCTGGTAGCTAGATTTTTTCAGTAAAATCCTCGACTGCTCTGTTCAGCTGGGCAAATTGTGGGCTATATTTGGGTTGGACAAGGAATATATTTTCTTTTCTTGAAATGTATGGTTCGGCAAGACTAGAGTAGTGAAATTATGGGTTTTCCTTCGGAAGTGGAATTAGATGAATATGTTGAAGAGTGCATAGGTTACACAATAGGTCTTCCTTTGTGTGGGAAAGCATTGGAAGAGAGACTAGCTCAAGCGGAGGAAGGACAGAGAGAGCTTCGTGAGAAAGTGGTGTATCTGAATCAGCGTCTCCAGCAGACGCAACAGAAACTGGACCGTGCCAGGGCGGAGGCTAATTTGAATGCACAAGCAGTGAGGAAATGCGTATGTGACAATGAGAAAGGTGCAGAACAGTATAGAAAGCTGCGTGACGAATGCACTCGTCTCGAGAACGAGTGTGCTCTTTATCTTCATGACAGAGAAGTGTTCATGGAGGCTGCTGATGAGGCAGAGGAAAGAGCCAATGAGGCAGAGGAACGTGTATTACAAGCCGAAAATAAAGTACAAGAATTAGCCTCTCAAGTTCAACATCTGAAACAGGGGATGTCACACTCTACAGCGAGCAAATTAAAGATGGCCGAAGAATTGAATTCACTGAGATGTAGAATTAGAGAGCTCGAGTCTGGGAATCACAGCTTGCAGGATGTGTCAACCATGACGAAAAAAATCAAGATGATTGTTTCACACACCATCAGATTGAGCAACTGGAAAGAGAAAATAGAGAGCTACGAGCCCTTTTAGATATTGCAACGTCTAGAAGTGAGGAACAGTTAAAGGAATGCCTAAATTCTAAGCAAAATGCTAAAGAAGACTCTTGGAGGGATCGGATGCTTGTTAATTCCCTTGTATCCTCAGCTGTTGCCAAAGGGTTATGCAAAGCCACAGAGCAAGAAAAGAAAGAAGCAATAGGCAGGATATATTCGAATCTTGAAGCTTTCGTCAAGGAAATGGGATCAGAGTTGCATGGAAGGCTAGAGGTGTCTGAGAAGAAAGTTCAATTATTGAACATAGAGGTACAGTCCCTGAGGCAAGAGAAAAACAGTATCAAGAGCAATCTTGAGAAGGCAGAGATTGAGGTGCAATTATTGGATGAGGAAAATAAGGAGCTAAGACTACTACTACGTGGGAAATATCAGGGGTCAGATGGATGTGTTATATCTCCTCTACAAACAAAAGTTAAAGTCTCCTGTCACCAGAAGTCACAGTTAAAGTCTACAGAAAACATTTGTGACATGAAGGGAAATGACATATTGCGGCAACCTCTTACACTTCTTGCGAAAAATGCTGCCGAATCAAGGGTGCACCAACCTCTTACAGCTCTTGCACAAAATGCTGCTGAATCAAGGGTGCAACATAGGTAGCAGGTGCCATATTAGTTAATGGTTGAAATATATATGGAGTTGTCGTCAAAGCTTGTGTTGTGTTAACTAATATTCATTGTCAAGGAGCATCTGTCCCTAGTTTATAACAGGCTACGTAAAGTTATGCCTGTTGATGGGAAATAGCCTTGAAAATTTGAATAAGATAAAATTTATGGTTTATTGTGTTTTGCATATTGAAAAATTCATGTGCACAGAAGAATATAAACATTTTCCCCCTTTCCCTGGCCCCCTCTTTTTTGGTTATGAAGTTCTGTTGTTGCAAGCAGTTTGTTATTGATGTAAGGTTCTTATGGCTCATCACATGCAACATGTCAGCCACATCATCTTCACTTTTGATTTTGGGATTTGTTAGAGACAATTCCGTTCTGTTGTTGCCCATTACAGAGTTCTCTTTTCGCTAAGCAGTAATCCACTCTTTTAGTTTGTTATTCACGTAAGGTTATTATTGCTCATCACATGCAACTTACGAGCCACATCATT

Ace file of the UniGene sp_v2.0_unigene2645 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	uncharacterized protein [Arabidopsis thaliana] emb\|CAB78030.1\| hypothetical protein [Arabidopsis thaliana] gb\|AAM67442.1\| unknown protein [Arabidopsis thaliana] gb\|AEE82717.1\| uncharacterized protein [Arabidopsis thaliana]	-	-	2.0e-19	34%
FL-Next	tr=Putative uncharacterized protein; Arabidopsis lyrata subsp. lyrata (Lyre-leaved rock-cress).	-	-	0.0	40%
Blast2go	uncharacterized protein	-	-	9.92614e-21	60%

Source	GOs	Term	Type	e value	Identity
Blast2go	membrane	GO:0016020	Cellular Component	9.92614e-21	60%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: tr_plants

Fln subject: D7M7P2

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 110 aas, putative chimeric sequence, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 218 - 342

Fln protein: MGFPSEVELDEYVEECIGYTIGLPLCGKALEERLAQAEEGQRELREKVVYLNQRLQQTQQKLDRARAEANLNAQAVRKCVCDNEKGAEQYRKLRDECTRLENECALYLHDREVFMEAADEAEERANEAEERVLQAENKVQELASQVQHLKQGMSHSTASKLKMAEELNSLRCRIRELESGNHSLQDVSTMTKKIKMIVSHTIRLSNWKEKIESYEPF*

Protein Length: 219

Fln nts: CGTCGTGACCTTGGCTGTCACTCAACAATCCACGATAGAAAGGAGATCGAGAGCTTATCTCAGCCACAAAACAAAAGAAAGAGTATTGTTTACCAAAAATAAGAAGTTGCAGCAGGACCATCCCGTCCCATAATTCCCAATTAATTCTCCGCAAATCCTCTGGTAGCTAGATTTTTTCAGTAAAATCCTCGACTGCTCTGTTCAGCTGGGCAAATTGTGGGCTATATTTGGGTTGGACAAGGAATATATTTTCTTTTCTTGAAATGTATGGTTCGGCAAGACTAGAGTAGTGAAATT_-_ATGGGTTTTCCTTCGGAAGTGGAATTAGATGAATATGTTGAAGAGTGCATAGGTTACACAATAGGTCTTCCTTTGTGTGGGAAAGCATTGGAAGAGAGACTAGCTCAAGCGGAGGAAGGACAGAGAGAGCTTCGTGAGAAAGTGGTGTATCTGAATCAGCGTCTCCAGCAGACGCAACAGAAACTGGACCGTGCCAGGGCGGAGGCTAATTTGAATGCACAAGCAGTGAGGAAATGCGTATGTGACAATGAGAAAGGTGCAGAACAGTATAGAAAGCTGCGTGACGAATGCACTCGTCTCGAGAACGAGTGTGCTCTTTATCTTCATGACAGAGAAGTGTTCATGGAGGCTGCTGATGAGGCAGAGGAAAGAGCCAATGAGGCAGAGGAACGTGTATTACAAGCCGAAAATAAAGTACAAGAATTAGCCTCTCAAGTTCAACATCTGAAACAGGGGATGTCACACTCTACAGCGAGCAAATTAAAGATGGCCGAAGAATTGAATTCACTGAGATGTAGAATTAGAGAGCTCGAGTCTGGGAATCACAGCTTGCAGGATGTGTCAACCATGACGAAAAAAATCAAGATGATTGTTTCACACACCATCAGATTGAGCAACTGGAAAGAGAAAATAGAGAGCTACGAGCCCTTTTAGATATTGCAACGTCTAGAAGTGAGGAACAGTTAAAGGAATGCCTAAATTCTAAGCAAAATGCTAAAGAAGACTCTTGGAGGGATCGGATGCTTGTTAATTCCCTTGTATCCTCAGCTGTTGCCAAAGGGTTATGCAAAGCCACAGAGCAAGAAAAGAAAGAAGCAATAGGCAGGATATATTCGAATCTTGAAGCTTTCGTCAAGGAAATGGGATCAGAGTTGCATGGAAGGCTAGAGGTGTCTGAGAAGAAAGTTCAATTATTGAACATAGAGGTACAGTCCCTGAGGCAAGAGAAAAACAGTATCAAGAGCAATCTTGAGAAGGCAGAGATTGAGGTGCAATTATTGGATGAGGAAAATAAGGAGCTAAGACTACTACTACGTGGGAAATATCAGGGGTCAGATGGATGTGTTATATCTCCTCTACAAACAAAAGTTAAAGTCTCCTGTCACCAGAAGTCACAGTTAAAGTCTACAGAAAACATTTGTGACATGAAGGGAAATGACATATTGCGGCAACCTCTTACACTTCTTGCGAAAAATGCTGCCGAATCAAGGGTGCACCAACCTCTTACAGCTCTTGCACAAAATGCTGCTGAATCAAGGGTGCAACATAGGTAGCAGGTGCCATATTAGTTAATGGTTGAAATATATATGGAGTTGTCGTCAAAGCTTGTGTTGTGTTAACTAATATTCATTGTCAAGGAGCATCTGTCCCTAGTTTATAACAGGCTACGTAAAGTTATGCCTGTTGATGGGAAATAGCCTTGAAAATTTGAATAAGATAAAATTTATGGTTTATTGTGTTTTGCATATTGAAAAATTCATGTGCACAGAAGAATATAAACATTTTCCCCCTTTCCCTGGCCCCCTCTTTTTTGGTTATGAAGTTCTGTTGTTGCAAGCAGTTTGTTATTGATGTAAGGTTCTTATGGCTCATCACATGCAACATGTCAGCCACATCATCTTCACTTTTGATTTTGGGATTTGTTAGAGACAATTCCGTTCTGTTGTTGCCCATTACAGAGTTCTCTTTTCGCTAAGCAGTAATCCACTCTTTTAGTTTGTTATTCACGTAAGGTTATTATTGCTCATCACATGCAACTTACGAGCCACATCATT

Fln Alignment:

Contig2645___ELDEYVEECIGYTIGLPLCGKALEERLAQAEEGQRELREKVVYLNQRLQQTQQKLDRARAEANLNAQAVRKCVCDNEKGAEQYRKLRDECTRLENECALYLHDREVFMXXXXXXXXXXXXXXERVLQAENKVQELASQVQHLKQ
D7M7P2______________EVEEYIIDTIDHSLGLPISMEALQKKLYTAEESQRRLREQYLALVSRLKEKDHVIDRVRSEASMNAQALKKFVDENQKLASECGNLLSQCKKLEKECLLYHQDRDALMEFGNESDERAREAEARVRELEDEIGRMSEEMQIFKR

SNPs (tot: 11)

Position	A %	C %	G %	T %	Probability
1702	87		12		0.992554
1727		12		87	0.992554
1985	80			20	0.995493
2050	33		66		0.997452
2051			33	66	0.997452
2053	25		75		0.996473
2055		33	66		0.997452
2057	33		66		0.997452
2060	75	25			0.996473
2063	33	66			0.997452
2066			66	33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain