SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene823

Length: 2197 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene823
GCTGCATTTCTGAAATATTTAATTGAGAATTCCAAGGGTGATAATTTTGAGGAACCATTGTTTCTTAATCTGACTGATAATGATGTTAAAGGAGATGGTTTTCCACAAGTTGATCGCATCAAAGAATTTGTTATTCAAGGCCTATTGGGCTTTATTGGCACTTCAGATGTAACTCCTTCAACTTATCTTCTTCATCTTGAGGTGGTTAATCTGCTTCTGGTGGCGATGTCGACTCAGCTTCATTCTGGGCCATCCCCAGGTCCAAAGGATGTCCATCCATTTATTGATGCTGCTATGGTGCAGGAAAGCGCATTGGTGGGTCCGTTTGTTCGCAGGTTATTGATTTCGTATATAACACGAGTACCAGCTCCTTTAAATGCTGCATATCATCTCAGTATTTCGAAAGTCAGTCAGCCTGGAGTTTTACGAAAAGTTGGTTCTGCAGCTGCTACTGTTGTATTGTTGCCATATTACACTTATAATTACCTTCTGAGTTCAAGTGGAGAAATATCAAAGCGACCCTTGGCAGATAACAGTGTTCTTTTACTTCTTGTTCTTGTCCATTATCGCAAAAGTTTGCTAGTTGAAGAGGCCACAGAAAATAAGCTGGATGCCAATGGTGGTGATACTGGAACTTTTGGGGAGAGCTCATGCTTTCATCATAATCCTTACGCCAGAGCCCTTGAAAGTGCAAGGGACATTGAATTTGATCGTGTAGATGTTGAAGGGAATGCACTAGGTGGCCCAATTGTGCGATTACCTTTTGCATCTTTATTCGACACCCTTGGGGCCTGCCTGACAGATGAGACTTCAATCTTGCTGCTCTACTCACTGGTCCATGGGAATTCTGACTTCTTGGAGTATGTGTTAGTACGAACTGATTTAGATACATTGCTGATACCAATTCTGGAAATGCTTTATGATGCACCACGGAGATCTTCAAATCAAATTTATATGTTGCTTATTATTCTTTTAATACTTAGTCAAGATTCATCTTTCAATGCTAGTATACATAAGTTGATACTGCCAAACGTTTCATGGTACCAAGAACGACTCTTACATCGAACATCACTTGGTTCACTGATGGTTGTGATCCTTATAAGAACAGTGCAGTATAATCTATTAAAACTGAGGGATGTTTATCTTCACACAAACTGCCTTGCAACATTGGCAAACATGGCCCCACATGTCCATAGATTGAGTGCATATGCATCACAAAGGCTTGTTAGCCTTTTTGACATGCTATCACGGAAGTACATAAAACTAGCAGAAGTTAACAGTAGCAAGATCATTGCACTGAAACATAATCCATCGGAGGGAAAAGATTTGGCTGAGGATATGCCAACTGAGCTACATATCTACACCGACTTCTTAAGAATTGTGTTGGAGATGATCAATGCTATTCTTACATATGCTTTGCCTCGAAATCCAGAGGTTGTGTATGCCATATTGCATAGACAGGAGCTGTTCAGGCCATTTAAGGACCATCCACGATTTTATGAACTAGTGGAGAACATTTATACAGTACTGGATTTTTTCAACAGTCGTATAGATGCTCAACATACAGATGGGGAATGGTCAGTAGAAAAGGTTCTTCAGGTCATAGTTGCTAATAGCTGGTCATGGCGCGGTGAAGGGATGAAGATGTTCACCCAGTTAAAATTCACCTATGAGCAGGAACTTCACCCAGAAGAGTTTTTTATTCCATATGTGTGGAAGTTAGTTGTATCTCAGAGTGGTGTGGATTTCAATCTCGACACCATTCACTTATTTCAAGCAAGGTCACCTGCGGAGGATGCTTCACAGGAGAAGACATTTGATGCACCAATCTCTCCTTTCATAGACGTCTAGAATGTATATATTACCCGAATAACCTTCGGTTTTTGGAATGACCCACCAATTGGTTCACTTGTATTCTTCCTGGACACGTAGCCATATCATATAATTTTTAAAGTAATTCTTTACACTGAACCTTTGAGTTGCCAAATACAAATGCAGCCTCAGATTGTTGTTTGCTATGTACAGAAGGCTTCTACGCAGATGTAAATTACAATTGTTTCAATTCTTTACACAATTGAATTTTCCAAACTTTCACGAGTTCTTGGTGAAGTGAGCTTGCTTTTAGTTGATATTCAAATTCTCTATCAGATATGCATGCATCTTTTGAACCCAATATGCGGTATTTTAGCAACGCATG

Ace file of the UniGene sp_v2.0_unigene823 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	pfam09742, Dymeclin, Dyggve-Melchior-Clausen syndrome protein	-	-	39%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	86%
Blast2go	uncharacterized protein	-	-	77%

Source	InterPros	Term	Type	e value	Identity
Sma3	Dymeclin	IPR019142	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5A9A6

Fln msg: your sequence is longer than subject: 542 - 129

Fln protein: MSTQLHSGPSPGPKDVHPFIDAAMVQESALVGPFVRRLLISYITRVPAPLNAAYHLSISKVSQPGVLRKVGSAAATVVLLPYYTYNYLLSSSGEISKRPLADNSVLLLLVLVHYRKSLLVEEATENKLDANGGDTGTFGESSCFHHNPYARALESARDIEFDRVDVEGNALGGPIVRLPFASLFDTLGACLTDETSILLLYSLVHGNSDFLEYVLVRTDLDTLLIPILEMLYDAPRRSSNQIYMLLIILLILSQDSSFNASIHKLILPNVSWYQERLLHRTSLGSLMVVILIRTVQYNLLKLRDVYLHTNCLATLANMAPHVHRLSAYASQRLVSLFDMLSRKYIKLAEVNSSKIIALKHNPSEGKDLAEDMPTELHIYTDFLRIVLEMINAILTYALPRNPEVVYAILHRQELFRPFKDHPRFYELVENIYTVLDFFNSRIDAQHTDGEWSVEKVLQVIVANSWSWRGEGMKMFTQLKFTYEQELHPEEFFIPYVWKLVVSQSGVDFNLDTIHLFQARSPAEDASQEKTFDAPISPFIDV*

Protein Length: 543

Fln nts: GCTGCATTTCTGAAATATTTAATTGAGAATTCCAAGGGTGATAATTTTGAGGAACCATTGTTTCTTAATCTGACTGATAATGATGTTAAAGGAGATGGTTTTCCACAAGTTGATCGCATCAAAGAATTTGTTATTCAAGGCCTATTGGGCTTTATTGGCACTTCAGATGTAACTCCTTCAACTTATCTTCTTCATCTTGAGGTGGTTAATCTGCTTCTGGTGGCG_-_ATGTCGACTCAGCTTCATTCTGGGCCATCCCCAGGTCCAAAGGATGTCCATCCATTTATTGATGCTGCTATGGTGCAGGAAAGCGCATTGGTGGGTCCGTTTGTTCGCAGGTTATTGATTTCGTATATAACACGAGTACCAGCTCCTTTAAATGCTGCATATCATCTCAGTATTTCGAAAGTCAGTCAGCCTGGAGTTTTACGAAAAGTTGGTTCTGCAGCTGCTACTGTTGTATTGTTGCCATATTACACTTATAATTACCTTCTGAGTTCAAGTGGAGAAATATCAAAGCGACCCTTGGCAGATAACAGTGTTCTTTTACTTCTTGTTCTTGTCCATTATCGCAAAAGTTTGCTAGTTGAAGAGGCCACAGAAAATAAGCTGGATGCCAATGGTGGTGATACTGGAACTTTTGGGGAGAGCTCATGCTTTCATCATAATCCTTACGCCAGAGCCCTTGAAAGTGCAAGGGACATTGAATTTGATCGTGTAGATGTTGAAGGGAATGCACTAGGTGGCCCAATTGTGCGATTACCTTTTGCATCTTTATTCGACACCCTTGGGGCCTGCCTGACAGATGAGACTTCAATCTTGCTGCTCTACTCACTGGTCCATGGGAATTCTGACTTCTTGGAGTATGTGTTAGTACGAACTGATTTAGATACATTGCTGATACCAATTCTGGAAATGCTTTATGATGCACCACGGAGATCTTCAAATCAAATTTATATGTTGCTTATTATTCTTTTAATACTTAGTCAAGATTCATCTTTCAATGCTAGTATACATAAGTTGATACTGCCAAACGTTTCATGGTACCAAGAACGACTCTTACATCGAACATCACTTGGTTCACTGATGGTTGTGATCCTTATAAGAACAGTGCAGTATAATCTATTAAAACTGAGGGATGTTTATCTTCACACAAACTGCCTTGCAACATTGGCAAACATGGCCCCACATGTCCATAGATTGAGTGCATATGCATCACAAAGGCTTGTTAGCCTTTTTGACATGCTATCACGGAAGTACATAAAACTAGCAGAAGTTAACAGTAGCAAGATCATTGCACTGAAACATAATCCATCGGAGGGAAAAGATTTGGCTGAGGATATGCCAACTGAGCTACATATCTACACCGACTTCTTAAGAATTGTGTTGGAGATGATCAATGCTATTCTTACATATGCTTTGCCTCGAAATCCAGAGGTTGTGTATGCCATATTGCATAGACAGGAGCTGTTCAGGCCATTTAAGGACCATCCACGATTTTATGAACTAGTGGAGAACATTTATACAGTACTGGATTTTTTCAACAGTCGTATAGATGCTCAACATACAGATGGGGAATGGTCAGTAGAAAAGGTTCTTCAGGTCATAGTTGCTAATAGCTGGTCATGGCGCGGTGAAGGGATGAAGATGTTCACCCAGTTAAAATTCACCTATGAGCAGGAACTTCACCCAGAAGAGTTTTTTATTCCATATGTGTGGAAGTTAGTTGTATCTCAGAGTGGTGTGGATTTCAATCTCGACACCATTCACTTATTTCAAGCAAGGTCACCTGCGGAGGATGCTTCACAGGAGAAGACATTTGATGCACCAATCTCTCCTTTCATAGACGTCTAG___AATGTATATATTACCCGAATAACCTTCGGTTTTTGGAATGACCCACCAATTGGTTCACTTGTATTCTTCCTGGACACGTAGCCATATCATATAATTTTTAAAGTAATTCTTTACACTGAACCTTTGAGTTGCCAAATACAAATGCAGCCTCAGATTGTTGTTTGCTATGTACAGAAGGCTTCTACGCAGATGTAAATTACAATTGTTTCAATTCTTTACACAATTGAATTTTCCAAACTTTCACGAGTTCTTGGTGAAGTGAGCTTGCTTTTAGTTGATATTCAAATTCTCTATCAGATATGCATGCATCTTTTGAACCCAATATGCGGTATTTTAGCAACGCATG

Fln Alignment:

Contig823___LFRPFKDHPRFYELVENIYTVLDFFNSRIDAQHTDGEWSVEKVLQVIVANSWSWRGEGMKMFTQLKFTYEQELHPEEFFIPYVWKLVVSQSGVDFNLDTIHLFQARSPAEDASQEKTFDAPISPFIDV
D5A9A6_____________LFQMLKIICSFY-----FFQVLDFFNSRIDAQHTDGEWSVEKVLQVIVANSWSWRGEGMKMFTQLKFTYEQELHPEEFFIPYVWKLVVSQSGVDFNLDTIHLFQARSPAEDASQEKTFDVPFSPFIDV

SNPs (tot: 52)

Position	A %	C %	G %	T %	Probability
1593		75		25	0.996473
1633	25			75	1.0
1649			88	11	0.923564
1650	88			11	0.923564
1660	87			12	0.992554
1661		87	12		0.992554
1663		87	12		0.992554
1671	12			87	0.992554
1682	12		87		0.992554
1684			12	87	0.992554
1685	12			87	0.992554
1686		12		87	0.992554
1758	90		10		0.90298
1837	90	10			0.90298
1841	10		90		0.90298
1863		88		11	0.923564
1864		88	11		0.923564
1865	11			88	0.923564
1867		88		11	0.923564
1872	88	11			0.923564
1878	88			11	0.923564
1880	11	88			0.923564
1884		88	11		0.923564
1887		12		87	0.992554
1888			75	25	1.0
1891	25	75			1.0
1892			25	75	1.0
1894			25	75	1.0
1895	25	75			1.0
1897	25			75	1.0
1902	28	71			1.0
1904			28	71	1.0
1905	71		28		1.0
1906			71	28	1.0
1909		14	85		0.993533
1910		71	28		1.0
1912		28		71	1.0
1913		71	28		1.0
1938	80	20			0.995493
1959	80		20		0.995493
2077			14	85	0.993533
2078	14			85	0.993533
2083	12	87			0.992554
2084		12	87		0.992554
2086			12	87	0.992554
2090		87	12		0.992554
2091			12	87	0.992554
2093		12	87		0.992554
2094	12			87	0.992554
2095		12	87		0.992554
2098	87	12			0.992554
2100	87		12		0.992554

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain