SustainPineDB

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Assembly info
Unigenes
SNPs
SSRs
Descriptions
GOs
ECs
KEGGs
InterPros
miRNAs

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene83201

Length: 371 nt

Origin of reads: Biogeco3 v1 Uagpf v2

UniGene Fasta

>sp_v2.0_unigene83201
CGTCGTGACCTTGGCTGTCACTCGGTGTTAATCTGCTTGTAGAAACTTACTTTGCTGATATCCCTGTTGAGACTTACAAGTAAGAGCCGGTCTTATTTGATCTTTGAAGTTTTTTAATTTGTCAGTATATTTTAGTATCTTAGTATCTTAGAAGAATGGATTTCGCGTTTCTAGATATCTGATGTTATGAAACATCGATCTTTGCAGGACCATTACTGGCTTGAAGGGTATCTCTGCTATTGGGTTTGATCTTATCCGTGGAACAAAGAATCTCGAACTCATCAAGGAGATTGGTTTCCCTGCTGACAAAATCTTGTTTGCAGGAGTAGTTGACGGCCGAAATATCTGGGCCAATGATCTGGCTGCTTCAT

Ace file of the UniGene sp_v2.0_unigene83201

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Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Vitamin-b12 independent methionine synthase, 5-methyltetrahydropteroyltriglutamate-homocysteine n=9 Tax=core eudicotyledons RepID=B9HQI3_POPTR	-	-	3.0e-18	62%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	100%
Blast2go	methionine synthase 2	-	-	2.44883e-26	83%

Source	ECs	Term	Type	e value	Identity
Blast2go	Methionine synthase.	EC:2.1.1.13	-	2.44883e-26	83%

Source	KEGGs	Term	e value	Identity
Blast2go	Cysteine and methionine metabolism	00270	2.44883e-26	83%
Blast2go	Selenocompound metabolism	00450	2.44883e-26	83%
Blast2go	One carbon pool by folate	00670	2.44883e-26	83%
Blast2go	Metabolic pathways	01100	2.44883e-26	83%
Blast2go	Biosynthesis of secondary metabolites	01110	2.44883e-26	83%

Source	GOs	Term	Type	e value	Identity
Blast2go	methionine biosynthetic process	GO:0009086	Biological Process	2.44883e-26	83%
Blast2go	response to salt stress	GO:0009651	Biological Process	2.44883e-26	83%
Blast2go	pollen exine formation	GO:0010584	Biological Process	2.44883e-26	83%
Blast2go	response to cadmium ion	GO:0046686	Biological Process	2.44883e-26	83%
Blast2go	methionine synthase activity	GO:0008705	Molecular Function	2.44883e-26	83%
Blast2go	fatty-acyl-CoA reductase (alcohol-forming) activity	GO:0080019	Molecular Function	2.44883e-26	83%
Blast2go	cytosol	GO:0005829	Cellular Component	2.44883e-26	83%
Blast2go	chloroplast envelope	GO:0009941	Cellular Component	2.44883e-26	83%
Blast2go	plasma membrane	GO:0005886	Cellular Component	2.44883e-26	83%
Blast2go	apoplast	GO:0048046	Cellular Component	2.44883e-26	83%

Source	InterPros	Term	Type	Identity
Sma3	Methionine synthase, vitamin-B12 independent	IPR002629	-	-
Sma3	Cobalamin-independent methionine synthase	IPR006276	-	-
Sma3	Cobalamin (vitamin B12)-independent methionine synthase MetE, N-terminal	IPR013215	-	-

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: D5A7T4

Fln msg: Separated hits, possible frame ERROR between 80 and 194, Unexpected stop codon in the beginning of your sequence, Distance to subject end: 459 aas, your sequence is shorter than subject: 123 - 766

Fln protein: S*PWLSLGVNLLVETYFADIPVETYxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxIDLCRTITGLKGISAIGFDLIRGTKNLELIKEIGFPADKILFAGVVDGRNIWANDLAAS

Protein Length: 124

Fln nts: CGTCGTGACCTTGGCTGTCACTCGGTGTTAATCTGCTTGTAGAAACTTACTTTGCTGATATCCCTGTTGAGACTTACAAxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxCATCGATCTTTGCAGGACCATTACTGGCTTGAAGGGTATCTCTGCTATTGGGTTTGATCTTATCCGTGGAACAAAGAATCTCGAACTCATCAAGGAGATTGGTTTCCCTGCTGACAAAATCTTGTTTGCAGGAGTAGTTGACGGCCGAAATATCTGGGCCAATGATCTGGCTGCTTCAT

Fln Alignment:

AllPineV4_rep_c143537___SLGVNLLVETYFADIPVETYKxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxIDLCRTITGLKGISAIGFDLIRGTKNLELIKEIGFPADKILFAGVVDGRNIWANDLAAS
D5A7T4_______________SLGVNLLVETYFADIPVETYKxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxVETYKTITGLKGISAIGFDFIRGSKTLGLIKEAGFPADKVLFAGVVDGRNIWANDLADS

SNPs (tot: 3)

Position	A %	G %	T %	Probability
146	25	75		0.996478
149	75		25	0.996478
180	50		50	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain