SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene78502

Length: 1970 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene78502
CGTCGTGACCTTGGCTGTCACTCAATTTTCTTGGTTACATTGCGAGAGGTTTCAGGCTCAGTCATATTGCAGCAACAATGCAGGGATCTCACATTTTGCTCGAGGAGCCCATTCGCATGGCCTCCATTCTCGAACCTTCCAAGCCGAATTTCTTTCCTGCGATGACAAAAATTATTGGCACTCTTGGACCACAGTCACGATCTGTTGAGGTGATTGCGGGCTGCCTTAAGGCTGGGATGTCTGTTGCCCGGTTTGATTTCTCGGGGGGAGATAATGACTATCACCAAGAAACTCTTGAAAATTTGAAGATTGCTGCAAAAGGAATGAATAAACTTTGTGCTATCATGCTCGATACAGTTGGTCCAGAGTTGCGAGTTTTTAACAGGAGTGAGGAGCCTGTTGCGCTTGAGATTGATGCGTTTGTGACTCTGACACCAGATCAGACCAAGGAAGCATCACCTGAGGTTTTGCCAATAAATTATGGTGGGCTTGCCAAGGCAGTGAAGAAAGGGGACACCATTTTTGTTGGTCAATATCTCTTTACAGGAAGTGAGACCACTTCTGTCTGGCTTGAGGTTGCAGAGATTAAAGATCAAGATATAGTTTGCTGTGTCAAAAACAGTGCAACTCTTGCTGGTTCATTATTTACAATGCATGCCACTCAAGTTCATATTGATCTTCCAACATTGAGCGACACAGATAAGCATAATATTGCAACTTGGGGAGTTCGCAACAACATTGACTTTCTTTCTTTGTCATACACACGACATGCTGAAGACATTATACAAACCCGCGCTTATCTTGATGAACTTGGGAATCTTAAACAGACACAAATATTTGCTAAGATTGACACTATAGAGGGCCTGGAGCACTTTGATGAGATCTTGCGACAAGCAGATGGTATTATTATTTCACGGGGTCACCTAGGGATAGATCTTCCACCAGAGAAGGTGTTTTTATTCCAGAAAGCCGCTGTCCACAAGTGTAACATGGCTGGAAAGCCCGTTGTAGTCACGCGTGTTGTTGACAGTATGTCGGGTAATTTACGGCCCACACGTGCAGAAGCAACTGATGTTGCAAATGCTGTTCTGGATGGTACAGATGCAATTCTGTTAGGTGCTGAAACTTACCGTGGATTATACCCTGTTCAGACTATCTCAATCGTAGGAAAGATCTGTGCAGAAGCGGAGAAAGTGTACAATCAAGCTATTCATTTTAAGAGGACTATGAAATATAGCAATGAACCAACGAGTCACTTGGAATCTATTGCAACTAGCGCAGTAAGTTCTGCAATCAAAGTGAATGCTTCGGTTATTGTTGTATTCACAGCAACTGGACAAACTGCTAGGTTAATAGCAAAGTACCGTCCTACCATGCCAGTTTTATCCGTTGTCATCCCAAGATTGACATCAAATCAGTTGAGATGGAGCTTTACTGGTGCATTTCAAGCAAGACAGACACTTGTAGTTAGGGGAGTTTTCCCTATGCTTGCAGATCCTCAACATCTGGCAGAATCAATTAATGAGACGAATGAGTCTGTTTTGAATCTTGCATTGGATCATGGTAAAACAACAGGGTTAATTAAACCTCATGATCGAATCGTTGTATGCCAAAAAATAGGGAACTCGGCTGTTGTTAAGATAGTCGAGCTTGAAGACTAGTCTGATACAGAAAGCAGTTGACAGATTTTGTCTCGTGTACAGAATATCATTAGGAATATGCCAAATTTTCTTAGTTATATCTGAACAGTGAATTTCGACGAGTTTTTCTTTTGCTTTTCAAATTCATCGGATAATCCATTGAAATTCACATGGATTTTATTGAGGAACCTGTAACAGTGAATTTCTAAAAGTATTTACTTAGATTAATTTATTTTAGATGAAAAGCTAGGTCCATTATTTATGAAATTTTGTTGTGTAACCTGCGGAATGAAAGTTGAACGATTAATATATCGTTTTCCCCGAAAATCT

Ace file of the UniGene sp_v2.0_unigene78502 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Pyruvate kinase n=2 Tax=Picea sitchensis RepID=A9NUL1_PICSI	-	-	89%
FL-Next	sp=Pyruvate kinase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	89%
Blast2go	pyruvate kinase	-	-	88%

Source	GOs	Term	Type	Identity
Blast2go	response to cadmium ion	GO:0046686	Biological Process	88%
Blast2go	catalytic activity	GO:0003824	Molecular Function	88%
Blast2go	plasma membrane	GO:0005886	Cellular Component	88%

Source	InterPros	Term	Type	Identity
Sma3	Pyruvate kinase	IPR001697	-	-
Sma3	Pyruvate/Phosphoenolpyruvate kinase	IPR015813	-	-
Sma3	Pyruvate kinase, alpha/beta	IPR015794	-	-
Sma3	Pyruvate kinase, barrel	IPR015793	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NUL1

Fln msg:

Fln protein: MQGSHILLEEPIRMASILEPSKPNFFPAMTKIIGTLGPQSRSVEVIAGCLKAGMSVARFDFSGGDNDYHQETLENLKIAAKGMNKLCAIMLDTVGPELRVFNRSEEPVALEIDAFVTLTPDQTKEASPEVLPINYGGLAKAVKKGDTIFVGQYLFTGSETTSVWLEVAEIKDQDIVCCVKNSATLAGSLFTMHATQVHIDLPTLSDTDKHNIATWGVRNNIDFLSLSYTRHAEDIIQTRAYLDELGNLKQTQIFAKIDTIEGLEHFDEILRQADGIIISRGHLGIDLPPEKVFLFQKAAVHKCNMAGKPVVVTRVVDSMSGNLRPTRAEATDVANAVLDGTDAILLGAETYRGLYPVQTISIVGKICAEAEKVYNQAIHFKRTMKYSNEPTSHLESIATSAVSSAIKVNASVIVVFTATGQTARLIAKYRPTMPVLSVVIPRLTSNQLRWSFTGAFQARQTLVVRGVFPMLADPQHLAESINETNESVLNLALDHGKTTGLIKPHDRIVVCQKIGNSAVVKIVELED*

Protein Length: 529

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTTTCTTGGTTACATTGCGAGAGGTTTCAGGCTCAGTCATATTGCAGCAACA_-_ATGCAGGGATCTCACATTTTGCTCGAGGAGCCCATTCGCATGGCCTCCATTCTCGAACCTTCCAAGCCGAATTTCTTTCCTGCGATGACAAAAATTATTGGCACTCTTGGACCACAGTCACGATCTGTTGAGGTGATTGCGGGCTGCCTTAAGGCTGGGATGTCTGTTGCCCGGTTTGATTTCTCGGGGGGAGATAATGACTATCACCAAGAAACTCTTGAAAATTTGAAGATTGCTGCAAAAGGAATGAATAAACTTTGTGCTATCATGCTCGATACAGTTGGTCCAGAGTTGCGAGTTTTTAACAGGAGTGAGGAGCCTGTTGCGCTTGAGATTGATGCGTTTGTGACTCTGACACCAGATCAGACCAAGGAAGCATCACCTGAGGTTTTGCCAATAAATTATGGTGGGCTTGCCAAGGCAGTGAAGAAAGGGGACACCATTTTTGTTGGTCAATATCTCTTTACAGGAAGTGAGACCACTTCTGTCTGGCTTGAGGTTGCAGAGATTAAAGATCAAGATATAGTTTGCTGTGTCAAAAACAGTGCAACTCTTGCTGGTTCATTATTTACAATGCATGCCACTCAAGTTCATATTGATCTTCCAACATTGAGCGACACAGATAAGCATAATATTGCAACTTGGGGAGTTCGCAACAACATTGACTTTCTTTCTTTGTCATACACACGACATGCTGAAGACATTATACAAACCCGCGCTTATCTTGATGAACTTGGGAATCTTAAACAGACACAAATATTTGCTAAGATTGACACTATAGAGGGCCTGGAGCACTTTGATGAGATCTTGCGACAAGCAGATGGTATTATTATTTCACGGGGTCACCTAGGGATAGATCTTCCACCAGAGAAGGTGTTTTTATTCCAGAAAGCCGCTGTCCACAAGTGTAACATGGCTGGAAAGCCCGTTGTAGTCACGCGTGTTGTTGACAGTATGTCGGGTAATTTACGGCCCACACGTGCAGAAGCAACTGATGTTGCAAATGCTGTTCTGGATGGTACAGATGCAATTCTGTTAGGTGCTGAAACTTACCGTGGATTATACCCTGTTCAGACTATCTCAATCGTAGGAAAGATCTGTGCAGAAGCGGAGAAAGTGTACAATCAAGCTATTCATTTTAAGAGGACTATGAAATATAGCAATGAACCAACGAGTCACTTGGAATCTATTGCAACTAGCGCAGTAAGTTCTGCAATCAAAGTGAATGCTTCGGTTATTGTTGTATTCACAGCAACTGGACAAACTGCTAGGTTAATAGCAAAGTACCGTCCTACCATGCCAGTTTTATCCGTTGTCATCCCAAGATTGACATCAAATCAGTTGAGATGGAGCTTTACTGGTGCATTTCAAGCAAGACAGACACTTGTAGTTAGGGGAGTTTTCCCTATGCTTGCAGATCCTCAACATCTGGCAGAATCAATTAATGAGACGAATGAGTCTGTTTTGAATCTTGCATTGGATCATGGTAAAACAACAGGGTTAATTAAACCTCATGATCGAATCGTTGTATGCCAAAAAATAGGGAACTCGGCTGTTGTTAAGATAGTCGAGCTTGAAGACTAG___TCTGATACAGAAAGCAGTTGACAGATTTTGTCTCGTGTACAGAATATCATTAGGAATATGCCAAATTTTCTTAGTTATATCTGAACAGTGAATTTCGACGAGTTTTTCTTTTGCTTTTCAAATTCATCGGATAATCCATTGAAATTCACATGGATTTTATTGAGGAACCTGTAACAGTGAATTTCTAAAAGTATTTACTTAGATTAATTTATTTTAGATGAAAAGCTAGGTCCATTATTTATGAAATTTTGTTGTGTAACCTGCGGAATGAAAGTTGAACGATTAATATATCGTTTTCCCCGAAAATCT

Fln Alignment:

AllPineV4_rep_c121180___MQGSHILLEEPIRMASILEPSKPNFFPAMTKIIGTLGPQSRSVEVIAGCLKAGMSVARFDFSGGDNDYHQETLENLKIAAKGMNKLCAIMLDTVGPELRVFNRSEEPVALEIDAFVTLTPDQTKEASPEVLPINYGGLAKAVKKGDTIFVGQYLFTGSETTSVWLEVAEIKDQDIVCCVKNSATLAGSLFTMHATQVHIDLPTLSDTDKHNIATWGVRNNIDFLSLSYTRHAEDIIQTRAYLDELGNLKQTQIFAKIDTIEGLEHFDEILRQADGIIISRGHLGIDLPPEKVFLFQKAAVHKCNMAGKPVVVTRVVDSMSGNLRPTRAEATDVANAVLDGTDAILLGAETYRGLYPVQTISIVGKICAEAEKVYNQAIHFKRTMKYSNEPTSHLESIATSAVSSAIKVNASVIVVFTATGQTARLIAKYRPTMPVLSVVIPRLTSNQLRWSFTGAFQARQTLVVRGVFPMLADPQHLAESINETNESVLNLALDHGKTTGLIKPHDRIVVCQKIGNSAVVKIV
A9NUL1_______________MQGAHLLLEEPIRMASILEPSKANFFHAMTKIVGTLGPNSRSVEVIEGCLKAGMSVARFDFSGGDNDYHQETLENLKIAVKEIKKLCAIMLDTVGPELQVFNRSEEPIVLEIDAFVTLTPDQSKEASSEVLPINYAGLAKTVTKGDTIFVGQYLFTGSETTSVWLEVAEIKDQDVVCCVKNSATLAGALFTMHISQVHIDLPTLSDTDKHVIATWGVRNNIDFLSLSYTRHAEDIRQTRAYLDELGNLQQTQIFAKIETIEGLEHFDEILQEADGIILSRGNLGIDLPPEKVFLFQKAAVHKCNMAGKPAVVTRVVDSMSGNLRPTRAEATDVANAVLDGTDAILLGAETFRGLYPFETISIVGKICAEAEKVYNQAVYFKRTMKHSNEPMSHLESIATTAVSAAINVKASVIVVFTASGRTARLIAKYRPTMPVLSVVIPRLTTNQLRWSFTGAFQARQTLIVRGVFPMLADPRHSAESINATNESVLEIALDHGKTAGLIKPHDRIVVCQKVGDSAVVKIL

SNPs (tot: 22)

Position	A %	C %	G %	T %	Probability
1546		80		20	0.995493
1564	20	80			0.995497
1568	20		80		0.995495
1576		80		20	0.995499
1592	20		80		0.995497
1596		80		20	0.995495
1606	80		20		0.995493
1607		80		20	0.995493
1610		80		20	0.995493
1645		25	75		0.996473
1666	75	25			0.99649
1692	60		40		1.0
1732	50	50			1.0
1744	50	50			1.0
1747	50		50		1.0
1774	40		60		1.0
1778			60	40	1.0
1816	25		75		0.996475
1961			33	66	0.997461
1964	66	33			0.997464
1967		66	33		0.997464
1969	66	33			0.997464

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain