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Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NXW9

Fln msg: Separated hits, possible frame ERROR between 230 and 347, Distance to subject end: 315 aas, atg_distance in limit (1-15): atg_distance = 9, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 134 - 460

Fln protein: HGDSVHSFLLLSVSARYSSAVNGEKLTNKEVPGFRANLIHIDSLLSPYSPGNISFPDRLQRAxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxEFLMKLAIGTPALSYSAILDTGSDLTWTQCKP

Protein Length: 135

Fln nts: ACATGGGACAATTTTGCAGAGCATTTGATGTCTGGTGCTAGC_-_ATGGGGATTCTGTTCATTCTTTTTTATTGCTTTCTGTGTCAGCCAGATACAGTTCTGCTGTAAATGGAGAGAAGCTAACAAACAAAGAGGTTCCTGGATTCAGAGCAAATCTGATACATATAGACTCGCTGCTGTCTCCATATAGTCCAGGGAATATTAGTTTCCCTGACCGCCTGCAAAGAGCAGTxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxAGAATTCCTCATGAAGCTGGCAATTGGCACGCCAGCTTTATCCTATTCTGCAATTCTTGACACAGGAAGCGACCTAACATGGACGCAGTGCAAGCCC

Fln Alignment:

AllPineV4_rep_c78053___VNGEKLTNKEVPGFRANLIHIDSLLSPYSPGNISFPDRLQRAVxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxEFLMKLAIGTPALSYSAILDTGSDLTWTQCKP
A9NXW9_______________VVGVRLQEEPLIGLRIDLVRTDSPLSPFSPGNISSTERFKRAIxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxEFLMKMAIGTPSLSFSAILDTGSDLTWTQCKP