SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene62403

Length: 1070 nt

Origin of reads: Biogeco1 v2 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene62403 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Os10g0543500 protein (Fragment) n=2 Tax=Oryza sativa RepID=Q0IW03_ORYSJ	-	-	46%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	85%
Blast2go	adipocyte plasma membrane-associated protein	-	-	68%

Source	InterPros	Term	Type	Identity
Sma3	NHL repeat	IPR001258	-	-
Sma3		IPR004141	-	-
Sma3	Six-bladed beta-propeller, TolB-like	IPR011042	-	-
Sma3	Strictosidine synthase, conserved region	IPR018119	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LRM9

Fln msg: Separated hits, possible frame ERROR between 651 and 701, Unexpected stop codon in the beginning of your sequence, your sequence is shorter than subject: 282 - 365

Fln protein: YVNHIRYKSVSTDQ*TQGILQVIITTFQRRSNSRLADGVDASREGAVYFTDASYKYGFHDYLFDILEYRPHGRLLKYEPSTKRTSVLLTDLYFPNGVALSAKQDFLVFCETSLYRCQKYWLKGPEKGTVESFIDNLPGLPDNIHYDGNGTFWIGFPTSRTLTWKIATRFPSVRHVLALMATLAPGAVTDAIVDEPGVLAVNEMGDPIALYSNPNSKxxxxxxxxxxxxxxxxxxDPIALYSNPNSKNQINTGLKKGECVYYGSLTLDYVGRVPLTQEIPIN*

Protein Length: 283

Fln nts: TATGTGAATCATATTAGGTATAAATCAGTTAGCACAGACCAGTAGACTCAAGGAATTCTTCAAGTAATCATAACTACATTCCAGAGGCGTAGTAACTCAAGATTGGCAGACGGAGTGGATGCAAGCAGAGAAGGGGCAGTTTATTTCACAGATGCCTCATATAAATATGGCTTCCATGACTACTTATTTGATATCTTGGAGTACCGACCACACGGCCGGTTGCTGAAATACGAGCCCTCCACCAAGAGAACGAGCGTACTTCTCACAGATCTTTACTTCCCCAACGGAGTGGCCCTCTCTGCCAAACAGGACTTCCTCGTCTTCTGTGAAACTTCACTGTATCGTTGCCAGAAGTACTGGTTAAAGGGGCCAGAGAAGGGCACAGTCGAATCTTTCATTGACAATCTCCCTGGACTTCCAGATAACATCCATTATGACGGGAACGGCACCTTCTGGATCGGATTTCCGACGAGCCGAACCTTAACCTGGAAAATTGCAACGAGGTTCCCGTCTGTGAGGCACGTGTTGGCTCTGATGGCGACACTGGCGCCAGGTGCAGTCACGGATGCTATAGTTGATGAGCCAGGTGTTTTGGCTGTGAATGAGATGGGCGATCCGATTGCGCTGTACTCCAATCCGAATTCGAAAAAxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxCGATCCGATTGCGCTGTACTCCAATCCGAATTCGAAAAATCAAATAAATACAGGATTGAAGAAGGGAGAGTGTGTGTACTATGGATCTCTTACTCTGGATTACGTTGGACGTGTTCCTCTCACCCAAGAGATACCAATTAATTAAAATTGGGTCCCAGGGATACATAGGACATTGGGAGACCAAATTTCCCTATTTTCCAAATTTCCCTAAAGATTTGTTAAAGTCACCTTAATCTAGGCAACCATGTTACTTGCAATTCCTTAAATATTAGTATGTTTTCTGTGTAAACTTTTACACGTTTTTCAGGTGAAATTAAAATAATGTAGAATTCGAGAATCTTTTGAAAAATTAAATGAATGGCAAGATTTGG

Fln Alignment:

AllPineV4_rep_c74649___RLADGVDASREGAVYFTDASYKYGFHDYLFDILEYRPHGRLLKYEPSTKRTSVLLTDLYFPNGVALSAKQDFLVFCETSLYRCQKYWLKGPEKGTVESFIDNLPGLPDNIHYDGNGTFWIGFPTSRTLTWKIATRFPSVRHVLALMATLAPGAVTDAIVDEPGVLAVNEMGDPIALYSNPNSKNxxxxxxxxxxxxxxxxxDPIALYSNPNSKNQINTGLKKGECVYYGSLTLDYVGRVPLTQEI
B8LRM9_______________KFADGVDASREGAVYFTDASYKYGFHDHLLDMLEYRPHGRLLKYDSSTKTTSVLLKDLYFPNGVALSAKQDFLVFCETTLYRCQKYWLEGPEKGTVESFIDNLPGLPDNIHYDGNGTFWIALATSRTLSWTIATKFPSVRHVLALIARLAPGAVIDAIVNEPSVLAVNEMGNPIALYADPNLSNxxxxxxxxxxxxxxxxxNPIALYADPNLSNQINSGLKMGDSLYYGSLTADYIGHVPLTEEI

SNPs (tot: 63)

Position	A %	C %	G %	T %	Probability
698	12			87	0.992554
747		90	10		0.90298
749		90	10		0.90298
750	90			10	0.90298
756	90	10			0.90298
758	10		90		0.90298
761			90	10	0.90298
763	90	10			0.90298
764	10		90		0.90298
765		10		90	0.90298
767	20	80			0.999996
769	10			80	0.959698
772		11		88	0.923564
773	11			88	0.923565
774	11			88	0.923564
781	88	11			0.923564
785		11	88		0.923564
790	14	85			0.993533
791	14	85			0.993533
795		14		85	0.993533
796	14	85			0.993533
797	14	85			0.993533
799			14	85	0.993533
801		14	85		0.993533
802	14		85		0.993533
803		14	85		0.993533
806	85	14			0.993533
817			83	16	0.994512
819			80	20	0.995493
822	80	20			0.995493
825		20		80	0.995493
831	20	80			0.995493
835		75	25		0.996473
848		25	75		0.996473
858		83	16		0.994512
864		83		16	0.994512
880	16			83	0.994512
885		83	16		0.994512
894	83			16	0.994512
902	83		16		0.994512
918		20		80	0.995493
926	80			20	0.995493
927			80	20	0.995493
933	20			80	0.995493
954	20			80	0.995493
955	20	80			0.995493
961	20		80		0.995493
973	25		75		0.996473
974	25			75	0.996473
975		75	25		0.996473
978	25	50			0.995533
979	25	75			0.996474
981	66		33		0.997452
982	33	33		33	0.997753
983		66		33	0.997452
984	33			66	0.997452
985	33			66	0.997452
988	33	33	33		0.997752
990		33	33	33	0.997752
991	66			33	0.997453
993	33		33	33	0.997751
995		66		33	0.997476
996	33	33	33		0.997748

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain