SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene61121

Length: 1385 nt

Origin of reads: Biogeco2 v2 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene61121 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Pyruvate decarboxylase 1 n=1 Tax=Lotus corniculatus RepID=Q84V95_LOTCO	-	-	80%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	95%
Blast2go	pyruvate decarboxylase	-	-	91%

Source	InterPros	Term	Type	Identity
Sma3	TPP-binding enzyme, conserved site	IPR000399	-	-
Sma3	Glycosyl transferase, family 20	IPR001830	-	-
Sma3	Pentatricopeptide repeat	IPR002885	-	-
Sma3	Thiamine pyrophosphate enzyme, C-terminal TPP-binding	IPR011766	-	-
Sma3	Thiamine pyrophosphate enzyme, central domain	IPR012000	-	-
Sma3	Thiamine pyrophosphate enzyme, N-terminal TPP-binding domain	IPR012001	-	-
Sma3	Pyruvate decarboxylase/indolepyruvate decarboxylase	IPR012110	-	-
Sma3	Gonadotropin, beta subunit, conserved site	IPR018245	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: C0PRN0

Fln msg: your sequence is shorter than subject: 253 - 409

Fln protein: VVTLAVTHFGCVLMKDFLEALAKKVKHNPTSLENYRRIFVPDGVPLKSAPNEPLRVNVLFKHIQGMLTDDTAVIAETGDSWFNCQKLKLPEKCGYEFQMQYGSIGWSVGATLGYAQATPEKRLISCIGDGSFQVTVQDVSTMIRNGQNPIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDALHNGEGKCWTAKVKTEEEAVEAIKTALGPKKDCLCFIEVIVHKDDTSKELLEWGSRVSAANSRPPNPQ*

Protein Length: 254

Fln nts: CGTCGTGACCTTGGCTGTCACTCATTTTGGGTGTGTGTTGATGAAAGATTTCTTAGAAGCCCTTGCAAAGAAGGTGAAACACAACCCCACATCTTTGGAAAATTATCGGCGTATTTTTGTTCCTGACGGTGTTCCCTTGAAAAGTGCACCAAATGAACCCTTAAGGGTCAATGTGTTATTCAAACACATCCAGGGCATGCTAACAGATGACACTGCTGTAATTGCTGAAACCGGTGACTCTTGGTTCAATTGTCAGAAGCTTAAATTGCCAGAAAAGTGTGGGTATGAGTTCCAAATGCAGTATGGATCGATAGGCTGGTCTGTAGGAGCAACACTAGGATATGCTCAAGCAACGCCAGAGAAGCGTTTAATATCATGCATTGGTGATGGAAGCTTTCAGGTGACAGTCCAGGATGTGTCTACTATGATTCGAAATGGGCAGAATCCTATCATTTTTCTGATTAATAATGGAGGATATACCATAGAAGTTGAAATTCATGATGGTCCATACAATGTCATAAAGAATTGGAACTACACCGGTTTAGTAGATGCCCTTCATAACGGTGAAGGGAAATGCTGGACTGCCAAGGTTAAGACGGAAGAAGAGGCTGTAGAGGCTATTAAAACTGCTCTAGGACCCAAAAAGGATTGCTTATGCTTCATAGAAGTTATTGTACACAAGGATGACACAAGTAAAGAGTTGCTTGAGTGGGGTTCCAGAGTCTCGGCAGCAAACAGCAGGCCGCCAAATCCTCAGTAG___GGAGGCTTTGAAAAGACGAGCAATTGGAAACACTTTTTTGCGGCTCATGTCTGAGATGTCATTCTTTCTCTGGTCTGGCTTTATAGGATCATGTTACAATTATTTATATCTGAATTTACTTTCTTATATCCGAGGTCAAGTTGTGGTCACACAACATAGGGCTACTTTTTGGTGACTGGTTAGCATGCTTAGTGTTTAGTGTCAAACTTAGTCGGAGCTATTTTTGCAATATAATTAAAATAGCAAAATTTCTGGTTATGGAATTTTGATCTAGATGATCTGTAATTGGTAACCCTTTATATTAGTTATATGGAATACCAGCAATTATATATATGGACAGTTTAATCAAACAGCTCAGCTGTTACTCTGTTACATTTAGTTTTTTTGTCAAATTTGATGTGATGGAATGTTCAAATTTGGTTGGTCACAGTGTTTTTATATCAAAAACTTGATATGAATGAATGTGGATATTCAATATTTGTTGGCACATGTAACAGATCTAATCAAACAGCTGTCACTCTGTTACAGTGATTTGCCTCGAACTTAATATTAAGGAATGAGGATGTTCAATGCTGGTTGGGCTATATAACTGATATTATTGAATGGCTGTTAATCTGGAATTCGC

Fln Alignment:

AllPineV4_rep_c67451___FGCVLMKDFLEALAKKVKHNPTSLENYRRIFVPDGVPLKSAPNEPLRVNVLFKHIQGMLTDDTAVIAETGDSWFNCQKLKLPEKCGYEFQMQYGSIGWSVGATLGYAQATPEKRLISCIGDGSFQVTVQDVSTMIRNGQNPIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDALHNGEGKCWXXXXXXXXXXXXXXXXALGPKKDCLCFIEVIVHKDDTSKELLEWGSRVSAANSRPPNPQ
C0PRN0_______________FGCVLMKDFLEALAKKVKHNPTSLENYRRIFVPEGVPLKSAPNEPLRVNVLFKHIQGMLTNETAVIAETGDSWFNCQKLKLPEKCGYEFQMQYGSIGWSVGATLGYAQAAPDKRVIACIGDGSFQVTVQDVSTMIRNGQNTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDALHNGEGKCWTVKVKTEEEVVEAIKTSLGPKKDCLCFIEVIVHKDDTSKELLEWGSRVSAANGRPPNPQ

SNPs (tot: 80)

Position	A %	C %	G %	T %	Probability
559	90			10	0.90298
563	90			10	0.90298
564	90		10		0.90298
569		10	90		0.90298
571	60		40		1.0
572		10		90	0.90298
573		10	90		0.90298
577			40	60	1.0
578		90	10		0.90298
579		30		70	1.0
580	30	70			1.0
582		10	30		1.0
584	70			30	1.0
586	90		10		0.90298
588		33		66	1.0
593		83		16	0.994512
594		83	16		0.994512
595	16		83		0.994512
598	80		20		0.995493
599	80	20			0.995493
600	80	20			0.995493
607		14	85		0.993533
609	14	14		71	0.984337
610	14	14		71	0.984337
611			28	71	1.0
612	14	85			0.993533
614	85		14		0.993533
616			16	83	0.994512
617			16	83	0.994512
618			83	16	0.994512
620	16			83	0.994512
623	16			83	0.994512
625			16	83	0.994512
651		20		80	0.995493
652	20			80	0.995493
654		20	80		0.995493
662	20	80			0.995493
663		20		80	0.995493
665	20	80			0.995493
666		20		80	0.995493
770	83			16	0.994512
774		80		20	0.995493
776		20	80		0.995493
777	20			80	0.995494
778			20	80	0.995494
779		20		80	0.995494
780		20		80	0.995494
784	80		20		0.995493
786		80		20	0.995493
788	20			80	0.995493
790			20	80	0.995493
905		85	14		0.993533
911	85	14			0.993533
912	14			85	0.993533
913			14	85	0.993533
914		14		85	0.993533
918		85	14		0.993533
919			14	85	0.993533
924		14		85	0.993533
926	14		85		0.993533
927		14		85	0.993533
928	85		14		0.993533
959			80	20	0.995493
964			20	80	0.995493
966	20			80	0.995493
970	20	80			0.995493
972			20	80	0.995493
973			20	80	0.995493
976	20		80		0.995493
977	80	20			0.995493
978	80		20		0.995493
979	20			80	0.995493
981	80		20		0.995493
1070		33		66	1.0
1226		25		75	0.996473
1227		25	75		0.996473
1228	75	25			0.996473
1255		33		66	0.997467
1256			33	66	0.997467
1258		33	66		0.997471

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain