SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene60367

Length: 2096 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene60367
CGTCGTGACCTTGTCTGTCACCCATGGAGCGGAAATCTTAATTTTTATTACTGGGCGGAAGGCAGGACAGACAGAACTCTCTGCATCTCGGCTCCCTACAGGTCGGAAACCCTTGGCCTGCGCATCCATGTTTGGTTTCGATCCATGGTTCTGCCCTTAACATTTCAGGTTTTTAAGGGGTTTCACGCTTAATTCTGAACGTATAGGGGTTTTAGGAGTGTTTACGCTTAATTTTAAGGCGAAATCGGTGCAGATCGGCGATCTCCGGCAGCAGATCTGGAGTTTTTTGGAGCTATATTTGTAAGCTTGATATCTTGGGTTTCTCTTCCGAGAAGAAAAAATGCCAAGTGGTGGTGGGTTTCGGGTGCTTCATCTAGTGAGGCCGTTTCTGTCAATCTTGCCCGAGGTCCAGACAGCTGAAAGAAAAGTGCCCTTCAGAGAGAAGGTTCTGTACACTGTAATCTCGCTTTTTATCTTTTTAGTGTGCAGTCAGTTGCCCCTATATGGCATACATTCAGCAACTGGGGCGGATCCATTCTACTGGATGCGTGTAATTCTGGCTTCTAATCGTGGAACTGTTATGGAGCTTGGTATTACCCCAATCGTTACTTCGGGCCTAGTAATGCAGCTTTTGGCAGGATCCAAGATAATTGAGGTGGATAACAGTGTTCGTGAAGATCGGGCTCTTCTTAATGGGGCTCAGAAGTTACTCGGTGTCCTCATCACAATAGGTGAGGCAGTTGCATACGTGCTGTCTGGAATGTATGGTGACATCCGGGACCTCGGAGCTGGCAATGCCATCCTCATTATTGTCCAGCTCTTTTTCGCTGGTATAATTGTGATATGTTTGGACGAGCTACTCCAGAAAGGTTATGGATTGGGATCTGGCATTTCATTATTCATTGCTACTAATATATGCGAGAGCATTATATGGAAGGCCTTTAGTCCCACTACAATCAATAGTGGACGAGGTGCAGAGTTCGAGGGAGCTGTTATTGCATTGTTCCATTTGTTGATAACCAGAACAGACAAGGTCCGGGCACTGCGGGAAGCTTTCTACCGGCAGAATCTTCCAAATGTTACCAACCTGCTTGCTACAGTTCTGGTTTTTCTCGTTGTCATATACTTCCAGGGTTTTCGTGTTGTTTTACCAGTGAGGTCAAAGAATGCCCGTGGGCAACAGGGATCATATCCTATCAAGTTATTCTATACCTCAAACATGCCAATTATTCTACAGTCAGCTCTTGTTTCAAACTTGTATTTTATTTCCCAGTTGCTTTACCGGAAGTACAGTGGAAACTTTCTTGTTAATCTGTTGGGCAAGTGGAAGGAGTCTGAATATTCATCAAGTGGTCAATTTGTTCCTGTAGGTGGTCTTGTTTATTACATCACTCCACCTTCCAGCTTGGCGGATATGGCAGCAAATCCTTTTCATGCACTGTTCTATTTAACTTTTATGCTGACAGCCTGTGCTCTATTCTCAAAGACATGGATTGAGGTATCTGGCTCCTCTGCAAGAGATGTGGCAAAACAGCTGAAGGAACAACAAATGGTTATGCCAGGCCACAGAGAATCTAATCTTCAGAAAGAGCTAAATCGTTACATACCAACAGCTGCGGCATTTGGTGGCATGTGCATTGGTGCCCTTACTGTCATGGCTGATTTTATGGGGGCCATTGGTTCTGGGACAGGAATTCTTCTTGCAGTGACTATTATTTATCAGTACTTTGAGACATTCGAGAAGGAAAGGGCCAGTGAACTAGGTTTCTTTGGTTTTTAATGCAGTCATTTGTATTTGACAATTTTAGAGGTTAGGATGCAGACTAAACTTTAGTTGGGCTAATGCTATGGCCCTTGAAGCATGTGAATGCTGGGGATTTTGATGCCCCTTCGAAGTTTTGCAGCCATCTTTTGATTTAGATATTAAATAGAATTCACAGAAGTCAAAACTGGCAGGCAGAAAAGTCCTGTGTGCTGTACTGACATTGAGATGTTTAGGGCACTTTGAAATAACTTCTTGGTTTTTGGGACAAAAGCAATTGTATAGTTGAAAGGCCATGTATTTGTGGTATTTGCAGTTCTTTGTTTAATCACGT

Ace file of the UniGene sp_v2.0_unigene60367 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	[U] COG0201 Preprotein translocase subunit SecY	-	-	54%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	97%
Blast2go	protein transport protein sec61 subunit alpha	-	-	96%

Source	GOs	Term	Type	e value	Identity
Blast2go	cell part	GO:0044464	Cellular Component	0.0	96%

Source	InterPros	Term	Type	e value	Identity
Sma3	SecY protein	IPR002208	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LKZ6

Fln msg:

Fln protein: MPSGGGFRVLHLVRPFLSILPEVQTAERKVPFREKVLYTVISLFIFLVCSQLPLYGIHSATGADPFYWMRVILASNRGTVMELGITPIVTSGLVMQLLAGSKIIEVDNSVREDRALLNGAQKLLGVLITIGEAVAYVLSGMYGDIRDLGAGNAILIIVQLFFAGIIVICLDELLQKGYGLGSGISLFIATNICESIIWKAFSPTTINSGRGAEFEGAVIALFHLLITRTDKVRALREAFYRQNLPNVTNLLATVLVFLVVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSALVSNLYFISQLLYRKYSGNFLVNLLGKWKESEYSSSGQFVPVGGLVYYITPPSSLADMAANPFHALFYLTFMLTACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGMCIGALTVMADFMGAIGSGTGILLAVTIIYQYFETFEKERASELGFFGF*

Protein Length: 481

Fln nts: CGTCGTGACCTTGTCTGTCACCCATGGAGCGGAAATCTTAATTTTTATTACTGGGCGGAAGGCAGGACAGACAGAACTCTCTGCATCTCGGCTCCCTACAGGTCGGAAACCCTTGGCCTGCGCATCCATGTTTGGTTTCGATCCATGGTTCTGCCCTTAACATTTCAGGTTTTTAAGGGGTTTCACGCTTAATTCTGAACGTATAGGGGTTTTAGGAGTGTTTACGCTTAATTTTAAGGCGAAATCGGTGCAGATCGGCGATCTCCGGCAGCAGATCTGGAGTTTTTTGGAGCTATATTTGTAAGCTTGATATCTTGGGTTTCTCTTCCGAGAAGAAAAA_-_ATGCCAAGTGGTGGTGGGTTTCGGGTGCTTCATCTAGTGAGGCCGTTTCTGTCAATCTTGCCCGAGGTCCAGACAGCTGAAAGAAAAGTGCCCTTCAGAGAGAAGGTTCTGTACACTGTAATCTCGCTTTTTATCTTTTTAGTGTGCAGTCAGTTGCCCCTATATGGCATACATTCAGCAACTGGGGCGGATCCATTCTACTGGATGCGTGTAATTCTGGCTTCTAATCGTGGAACTGTTATGGAGCTTGGTATTACCCCAATCGTTACTTCGGGCCTAGTAATGCAGCTTTTGGCAGGATCCAAGATAATTGAGGTGGATAACAGTGTTCGTGAAGATCGGGCTCTTCTTAATGGGGCTCAGAAGTTACTCGGTGTCCTCATCACAATAGGTGAGGCAGTTGCATACGTGCTGTCTGGAATGTATGGTGACATCCGGGACCTCGGAGCTGGCAATGCCATCCTCATTATTGTCCAGCTCTTTTTCGCTGGTATAATTGTGATATGTTTGGACGAGCTACTCCAGAAAGGTTATGGATTGGGATCTGGCATTTCATTATTCATTGCTACTAATATATGCGAGAGCATTATATGGAAGGCCTTTAGTCCCACTACAATCAATAGTGGACGAGGTGCAGAGTTCGAGGGAGCTGTTATTGCATTGTTCCATTTGTTGATAACCAGAACAGACAAGGTCCGGGCACTGCGGGAAGCTTTCTACCGGCAGAATCTTCCAAATGTTACCAACCTGCTTGCTACAGTTCTGGTTTTTCTCGTTGTCATATACTTCCAGGGTTTTCGTGTTGTTTTACCAGTGAGGTCAAAGAATGCCCGTGGGCAACAGGGATCATATCCTATCAAGTTATTCTATACCTCAAACATGCCAATTATTCTACAGTCAGCTCTTGTTTCAAACTTGTATTTTATTTCCCAGTTGCTTTACCGGAAGTACAGTGGAAACTTTCTTGTTAATCTGTTGGGCAAGTGGAAGGAGTCTGAATATTCATCAAGTGGTCAATTTGTTCCTGTAGGTGGTCTTGTTTATTACATCACTCCACCTTCCAGCTTGGCGGATATGGCAGCAAATCCTTTTCATGCACTGTTCTATTTAACTTTTATGCTGACAGCCTGTGCTCTATTCTCAAAGACATGGATTGAGGTATCTGGCTCCTCTGCAAGAGATGTGGCAAAACAGCTGAAGGAACAACAAATGGTTATGCCAGGCCACAGAGAATCTAATCTTCAGAAAGAGCTAAATCGTTACATACCAACAGCTGCGGCATTTGGTGGCATGTGCATTGGTGCCCTTACTGTCATGGCTGATTTTATGGGGGCCATTGGTTCTGGGACAGGAATTCTTCTTGCAGTGACTATTATTTATCAGTACTTTGAGACATTCGAGAAGGAAAGGGCCAGTGAACTAGGTTTCTTTGGTTTTTAA___TGCAGTCATTTGTATTTGACAATTTTAGAGGTTAGGATGCAGACTAAACTTTAGTTGGGCTAATGCTATGGCCCTTGAAGCATGTGAATGCTGGGGATTTTGATGCCCCTTCGAAGTTTTGCAGCCATCTTTTGATTTAGATATTAAATAGAATTCACAGAAGTCAAAACTGGCAGGCAGAAAAGTCCTGTGTGCTGTACTGACATTGAGATGTTTAGGGCACTTTGAAATAACTTCTTGGTTTTTGGGACAAAAGCAATTGTATAGTTGAAAGGCCATGTATTTGTGGTATTTGCAGTTCTTTGTTTAATCACGT

Fln Alignment:

AllPineV4_rep_c63056___MPSGGGFRVLHLVRPFLSILPEVQTAERKVPFREKVLYTVISLFIFLVCSQLPLYGIHSATGADPFYWMRVILASNRGTVMELGITPIVTSGLVMQLLAGSKIIEVDNSVREDRALLNGAQKLLGVLITIGEAVAYVLSGMYGDIRDLGAGNAILIIVQLFFAGIIVICLDELLQKGYGLGSGISLFIATNICESIIWKAFSPTTINSGRGAEFEGAVIALFHLLITRTDKVRALREAFYRQNLPNVTNLLATVLVFLVVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSALVSNLYFISQLLYRKYSGNFLVNLLGKWKESEYSSSGQFVPVGGLVYYITPPSSLADMAANPFHALFYLTFMLTACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGMCIGALTVMADFMGAIGSGTGILLAVTIIYQYFETFEKERASELGFFGF
B8LKZ6_______________MPSGG-FRVLHLVRPFLSILPEVQTADRKVPFREKVLYTVISLFIFLVCSQLPLYGIHSATGADPFYWMRVILASNRGTVMELGITPLVTSGLVMQLLAGSKIIEVDNSVREDRELLNGAQKLLGVLITIGEAVAYVLSGMYGDITDLGAGNAILIIVQLFFAGIIVICLDELLQKGYGLGSGISLFIATNICESIIWKAFSPTTINSGRGAEFEGAVIALFHLLITRTDKVRALREAFYRQNLPNVTNLLATVLVFLIVIYFQGFRVVLPVRSKNARGQQGSYPIKLFYTSNMPIILQSALVSNLYFISQLLYRKYSGNFLVNMLGKWKESEYSSSGQSIPVGGLVYYITPPSSLAEMAANPFHALFYLTFMLTACALFSKTWIEVSGSSARDVAKQLKEQQMVMPGHRESNLQKELNRYIPTAAAFGGMCIGALTVMADFMGAIGSGTGILLAVTIIYQYFETFEKERASELGFFGF

SNPs (tot: 51)

Position	A %	C %	G %	T %	Probability
1406		14		85	1.0
1666	82		5		0.999919
1850	91		8		0.999807
1931		89		10	0.999919
1932	5	84	10		0.999846
1933	10		5	84	0.999846
1934	84	5	10		0.999846
1938	10		89		0.999919
1939			15	84	1.0
1940		10		89	0.999919
1943	9		90		0.999872
1944	90		9		0.999872
1945	85	4	9		0.999726
1947		10		90	0.999898
1949	90	10			0.999898
2037		92		7	0.999532
2049		7	92		0.999532
2052	92		7		0.999532
2061	21		78		1.0
2070	7		88	3	0.998862
2073		92		7	0.999664
2085	8	92			0.999718
2091	90		10		0.999898
2093	72		11		1.0
2095	88	11			0.999952
2097	88		11		0.999952
2098		11		88	0.999952
2099		11		88	0.999952
2101	82			17	1.0
2103	88		11		0.999952
2104			17	82	1.0
2105		11		88	0.999952
2106	11		17	70	1.0
2107	17	82			1.0
2108		88	11		0.999952
2109	12		68		1.0
2110	18	81			1.0
2111			33	66	1.0
2115	7			78	0.999974
2116	16	8	50	25	1.0
2118	27	9	18	9	1.0
2119		20	60	20	0.999999
2120	18			81	0.999994
2121		9	18		0.999994
2122		18	54	27	1.0
2123	80	20			0.999996
2125	44	22			1.0
2126		22	66		0.999999
2127	50	37		12	1.0
2128		62		37	1.0
2129	50		33	16	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain