SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene33363

Length: 1998 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene33363
CGTCGTGACCTTGGCTGTCACTCAGTGGTCTAACCATACCTTCGTCGTGACCTTGGCGGAGTTTCAATTTTGGAGGATATCCGTCTATCAGCTGCGATGGAGATGGATATGGAGAAGTTACGCATCAGACAGCAAGTGTTGTTGGACCATCTGAGGCCTTCTCGTTTTCCCAAGGTTTCCACAGATGTGGTGGCTTCAGTATGTGCTGCTGGAGACAGTGCAGCTTATGAAAGATCTGCTGAGTTTGGTGATGATGTTGTCATTGTTGCAGCTTATCGTACCCCTATATGCAAGGCTAAGCGGGGGGGATTTAAGGACACTCTTCCTGATGACTTGCTTGCTCCCGTTTTGAAGGCATTGATTGATAAGACTGGAGTTAATCCAAATGAAGTTGGTGATATCGTTGTGGGTACTGTTCTTGCACCTGGCTCTCAACGTGCTAGTGAGTGCAGGATGGCATCTTTTTATGCTGGATTCCCAGAGACTGTACCTGTCAGGACTGTCAATAGACAATGCTCTTCAGGTCTCCAAGCTGTTGCAGATGTTGCAGCTGCAATTAAAGCTGGTTTTTATGACATTGGCATTGGTGCAGGATTGGAGTCAATGACTGTAAACCCTATGGCGTGGGAAGGCTCTGTCAATTCAAAGGTTGAAATTTTCAAACAAGCACAAGATTGTCTTCTGCCCATGGGTATCACCTCTGAAAATGTCGCTGAGCGGTACGGAGTTTCACGTCAAGACCAGGATCAAGCAGCGGTTGAATCTCATAGGCGTGCAGCAGCAGCAACAGCTTCTGGGAAGTTCAAAGATGAGATCATCCCTGTTGAAACCAAGATTGTTGATCCTAAAACTGGGGATGAGAAGCCCGTCACGGTTTCTGTTGATGATGGCATTCGTCCTACTGCTTCTCTTTCTGATTTAGCGAAACTGAAACCTGCATTTAAGAAGAGTGGAAGCACTACTGCTGGAAATTCAAGCCAAGTAAGTGATGGAGCTGGTGCTGTATTGCTGATGAAGAGGAAAGTGGCTATACAGAAGGGTTTGCCTATTTTGGGTGTATTCCCAGCAGCGGTGAAGGCAGCTGGGCTAGAAATTGATGATATTGATCTATTTGAGATAAATGAGGCATTTGCCTCGCAATTTACATACTGTAGACAGAAATTGGAACTAGACCCTGAAAAAGTAAATGTGAATGGAGGAGCTATTGCCCTTGGTCATCCCCTTGGAGCAACAGGGGCTCGTTGTGTGGCAACCTTACTGCATGAAATGAAACGCCGTGGAAAAGACTGCAGGTTTGGAGTTATATCGATGTGTATAGGCACTGGGATGGGGGCAGCAGCAGTATTTGAACGTGGCAGTGCTGTAGATCAGCTGTGCAATGCTCGGCCAATCAAGGATCATACCTCGCTGTCAAGAGATGCACGATAACAAACAGTGTTTTCTTGGAGTCTTGAAGGCTTTTCAATTATCTCCTTTTTAATGGACTCACTTCATGGGTTCTGGTTAACCTTTTAATGAGATGGCCTTTCTGGCCTATGGCCTGTATTGAGATTTTATGGCTTATGACATTTTCCATAGCATGGTTTTGGAATGGAATATAATAATGGCCATTTTTTAAATATTTAAAGTAAAATCTTGTCTGAAATATTGTGAACGAGACAAAAATGATGCATATGATTTGAAATGCTACTTTATGGACCTTTCGTAGCCATATGAAATATAAACTGGCACATGCATCTTAAATCGAGAACTATTCAGCACTGAGGAATAAAATGAGATTGAGCTGTGAACCTCTTGGCACTTAATAGATTGGAAGTTAAGGTGAATCCATGCTCGTGTGTAATTGGTTCTCTTTTATGTGGGTAGGAGCATTGCATGAAGACACACATGGAGTAAATGTATATCTGCTCCACCTGAACTGTAGAGCACAGTGTTTCTCTTGACTTGATCGCTAGTGTAATGAGAATGGAAGGAATGAATGAGAATTTTGATGTTAAG

Ace file of the UniGene sp_v2.0_unigene33363 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	3-ketoacyl-CoA thiolase 2 [Arabidopsis thaliana] sp\|Q56WD9.2\|THIK2_ARATH RecName: Full=3-ketoacyl-CoA thiolase 2, peroxisomal; AltName: Full=Acetyl-CoA acyltransferase 2; AltName: Full=Beta-ketothiolase 2; AltName: Full=Peroxisomal 3-oxoacyl-CoA thiolase	-	-	72%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	90%
Blast2go	3-ketoacyl- thiolase	-	-	86%

Source	ECs	Term	Type	e value	Identity
Blast2go	Acetyl-CoA C-acyltransferase.	EC:2.3.1.16	-	0.0	86%

Source	KEGGs	Term	Identity
Blast2go	Fatty acid elongation in mitochondria	00062	86%
Blast2go	Fatty acid metabolism	00071	86%
Blast2go	Valine, leucine and isoleucine degradation	00280	86%
Blast2go	Geraniol degradation	00281	86%
Blast2go	Benzoate degradation	00362	86%
Blast2go	alpha-Linolenic acid metabolism	00592	86%
Blast2go	Ethylbenzene degradation	00642	86%
Blast2go	Biosynthesis of unsaturated fatty acids	01040	86%
Blast2go	Biosynthesis of plant hormones	01070	86%
Blast2go	Metabolic pathways	01100	86%
Blast2go	Biosynthesis of secondary metabolites	01110	86%

Source	GOs	Term	Type	Identity
Blast2go	positive regulation of abscisic acid mediated signaling pathway	GO:0009789	Biological Process	86%
Blast2go	glyoxysome organization	GO:0010111	Biological Process	86%
Blast2go	metabolic process	GO:0008152	Biological Process	86%
Blast2go	acetyl-CoA C-acyltransferase activity	GO:0003988	Molecular Function	86%
Blast2go	peroxisome	GO:0005777	Cellular Component	86%
Blast2go	mitochondrion	GO:0005739	Cellular Component	86%
Blast2go	nucleolus	GO:0005730	Cellular Component	86%
Blast2go	vacuolar membrane	GO:0005774	Cellular Component	86%
Blast2go	chloroplast	GO:0009507	Cellular Component	86%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: C0PQ57

Fln msg: your sequence is shorter than subject: 444 - 462

Fln protein: MEMDMEKLRIRQQVLLDHLRPSRFPKVSTDVVASVCAAGDSAAYERSAEFGDDVVIVAAYRTPICKAKRGGFKDTLPDDLLAPVLKALIDKTGVNPNEVGDIVVGTVLAPGSQRASECRMASFYAGFPETVPVRTVNRQCSSGLQAVADVAAAIKAGFYDIGIGAGLESMTVNPMAWEGSVNSKVEIFKQAQDCLLPMGITSENVAERYGVSRQDQDQAAVESHRRAAAATASGKFKDEIIPVETKIVDPKTGDEKPVTVSVDDGIRPTASLSDLAKLKPAFKKSGSTTAGNSSQVSDGAGAVLLMKRKVAIQKGLPILGVFPAAVKAAGLEIDDIDLFEINEAFASQFTYCRQKLELDPEKVNVNGGAIALGHPLGATGARCVATLLHEMKRRGKDCRFGVISMCIGTGMGAAAVFERGSAVDQLCNARPIKDHTSLSRDAR*

Protein Length: 445

Fln nts: CGTCGTGACCTTGGCTGTCACTCAGTGGTCTAACCATACCTTCGTCGTGACCTTGGCGGAGTTTCAATTTTGGAGGATATCCGTCTATCAGCTGCG_-_ATGGAGATGGATATGGAGAAGTTACGCATCAGACAGCAAGTGTTGTTGGACCATCTGAGGCCTTCTCGTTTTCCCAAGGTTTCCACAGATGTGGTGGCTTCAGTATGTGCTGCTGGAGACAGTGCAGCTTATGAAAGATCTGCTGAGTTTGGTGATGATGTTGTCATTGTTGCAGCTTATCGTACCCCTATATGCAAGGCTAAGCGGGGGGGATTTAAGGACACTCTTCCTGATGACTTGCTTGCTCCCGTTTTGAAGGCATTGATTGATAAGACTGGAGTTAATCCAAATGAAGTTGGTGATATCGTTGTGGGTACTGTTCTTGCACCTGGCTCTCAACGTGCTAGTGAGTGCAGGATGGCATCTTTTTATGCTGGATTCCCAGAGACTGTACCTGTCAGGACTGTCAATAGACAATGCTCTTCAGGTCTCCAAGCTGTTGCAGATGTTGCAGCTGCAATTAAAGCTGGTTTTTATGACATTGGCATTGGTGCAGGATTGGAGTCAATGACTGTAAACCCTATGGCGTGGGAAGGCTCTGTCAATTCAAAGGTTGAAATTTTCAAACAAGCACAAGATTGTCTTCTGCCCATGGGTATCACCTCTGAAAATGTCGCTGAGCGGTACGGAGTTTCACGTCAAGACCAGGATCAAGCAGCGGTTGAATCTCATAGGCGTGCAGCAGCAGCAACAGCTTCTGGGAAGTTCAAAGATGAGATCATCCCTGTTGAAACCAAGATTGTTGATCCTAAAACTGGGGATGAGAAGCCCGTCACGGTTTCTGTTGATGATGGCATTCGTCCTACTGCTTCTCTTTCTGATTTAGCGAAACTGAAACCTGCATTTAAGAAGAGTGGAAGCACTACTGCTGGAAATTCAAGCCAAGTAAGTGATGGAGCTGGTGCTGTATTGCTGATGAAGAGGAAAGTGGCTATACAGAAGGGTTTGCCTATTTTGGGTGTATTCCCAGCAGCGGTGAAGGCAGCTGGGCTAGAAATTGATGATATTGATCTATTTGAGATAAATGAGGCATTTGCCTCGCAATTTACATACTGTAGACAGAAATTGGAACTAGACCCTGAAAAAGTAAATGTGAATGGAGGAGCTATTGCCCTTGGTCATCCCCTTGGAGCAACAGGGGCTCGTTGTGTGGCAACCTTACTGCATGAAATGAAACGCCGTGGAAAAGACTGCAGGTTTGGAGTTATATCGATGTGTATAGGCACTGGGATGGGGGCAGCAGCAGTATTTGAACGTGGCAGTGCTGTAGATCAGCTGTGCAATGCTCGGCCAATCAAGGATCATACCTCGCTGTCAAGAGATGCACGATAA___CAAACAGTGTTTTCTTGGAGTCTTGAAGGCTTTTCAATTATCTCCTTTTTAATGGACTCACTTCATGGGTTCTGGTTAACCTTTTAATGAGATGGCCTTTCTGGCCTATGGCCTGTATTGAGATTTTATGGCTTATGACATTTTCCATAGCATGGTTTTGGAATGGAATATAATAATGGCCATTTTTTAAATATTTAAAGTAAAATCTTGTCTGAAATATTGTGAACGAGACAAAAATGATGCATATGATTTGAAATGCTACTTTATGGACCTTTCGTAGCCATATGAAATATAAACTGGCACATGCATCTTAAATCGAGAACTATTCAGCACTGAGGAATAAAATGAGATTGAGCTGTGAACCTCTTGGCACTTAATAGATTGGAAGTTAAGGTGAATCCATGCTCGTGTGTAATTGGTTCTCTTTTATGTGGGTAGGAGCATTGCATGAAGACACACATGGAGTAAATGTATATCTGCTCCACCTGAACTGTAGAGCACAGTGTTTCTCTTGACTTGATCGCTAGTGTAATGAGAATGGAAGGAATGAATGAGAATTTTGATGTTAAG

Fln Alignment:

AllPineV4_c20284___MDMEKLRIRQQVLLDHLRPSRFPKVSTDVVASVCAAGDSAAYERSAEFGDDVVIVAAYRTPICKAKRGGFKDTLPDDLLAPVLKALIDKTGVNPNEVGDIVVGTVLAPGSQRASECRMASFYAGFPETVPVRTVNRQCSSGLQXXXXXXXXXXAGFYDIGIGAGLESMTVNPMAWEGSVNSKVEIFKQAQDCLLPMGITSENVAERYGVSRQDQDQXXXXXXXXXXXXXXSGKFKDEIIPVETKIVDPKTGDEKPVTVSVDDGIRPTASLSDLAKLKPAFKKSGSTTAGNSSQVSDGAGAVLLMKRKVAIQKGLPILGVF---------------------PAAVKAAGLEIDDIDLFEINEAFASQFTYCRQKLELDPEKVNVNGGAIALGHPLGATGARCVATLLHEMKRRGKDCRFGVISMCIGTGMGAAAVFERGSAVDQLCNARPIKDHTSLSRDAR
C0PQ57_______________MEMEKLRIRQQVLLDHLRPARFPKASTDLVASVCSAGDSAAYERAAEFGDDVVIVAAYRTPICKAKRGGFKDTLPDDLLAPVLKALIDKTGINPNEVRDIVVGTVLAPGSQRASECRMASFYAGFPETVPVRTVNRQCSSGLQAVADVAAAIKAGFYDIGIGAGLESMTANPMAWEGSVNPRVEIFKQAQDCLLPMGITSENVAERYGISRQDQDQAAVESHRRAAAATAAGKFKDEIIPVETKIVDPKTGDEKPVRISVDDGIRPTASLSDLAKLKPAFKKGGSTTAGNSSQVSDGAGAVLLMKRTVAMQKGLPILGVFRSFTAVGVDPAVMGVGPAVAIPAAVKAAGLEIDDIDLFEINEAFASQFTYCSQKLELDPEKVNVNGGALALGHPLGATGARCVATLLHEMKRRGKDCRFGVISMCIGTGMGAAAVFERGSAVDQLCNARPIKDHTSLSKDAQ

SNPs (tot: 100)

Position	A %	C %	G %	T %	Probability
94		50	50		1.0
177	88		11		0.991528
370	85		14		0.999981
371	14			85	0.999981
372			14	85	0.999981
374			14	85	0.999981
377	13			86	0.999973
378			14	85	0.999981
379		14		85	0.999981
380	87	12			0.999964
384	86		13		0.999973
385			13	86	0.999973
387		13		86	0.999973
389	86	13			0.999973
390	86		13		0.999973
392	86	13			0.999973
393	86		13		0.999973
429		13		86	0.999973
430	13		86		0.999973
432	86	13			0.999973
433	86	13			0.999973
436			10	90	0.999898
442			15	85	1.0
444	90	10			0.999898
487		88	11		0.999937
488	11			88	0.999937
680		92	1		0.99999
721		91	8		1.0
724		91	8		1.0
730		9		90	1.0
732	10			85	1.0
733	10			90	1.0
734			10	90	1.0
735			5	82	1.0
736		87	5	7	0.999996
741	12		87		1.0
783	6			93	0.999153
787	3		9	84	0.999999
791			9	90	1.0
794	93		6		0.999153
796		6	93		0.999264
798			88	11	1.0
799		11	88		1.0
801		12		88	1.0
805		88	12		1.0
879	10			90	0.90298
884	10		90		0.90298
887	90	10			0.90298
890			10	90	0.90298
892		90		10	0.90298
894	10			90	0.90298
897		10		90	0.90298
898			10	90	0.90298
939		16	83		0.994512
943	16			83	0.994512
951		20	80		0.995494
958	60			40	1.0
960	60		40		1.0
963	16	83			0.994512
965		83	16		0.994512
966	83		16		0.994512
1671			10	90	0.90298
1675	85	14			0.993533
1676	85		14		0.993533
1691	83		16		0.994512
1709	16			83	0.994512
1712		83	16		0.994512
1713		16		83	0.994512
1714		16		83	0.994512
1717	83		16		0.994512
1719			16	83	0.994512
1722		83		16	0.994513
1724	16	83			0.994514
1726		83	16		0.994514
1727	16	83			0.994512
1844		90		10	0.90298
1845		90	10		0.90298
1849			10	90	0.90298
1851		10		90	0.90298
1852	11		88		0.991528
1853	10			90	0.90298
1856	90			10	0.90298
1857	90	10			0.90298
1860		90	10		0.90298
1971			14	85	0.993535
1972	14		85		0.993533
1974	57		42		1.0
1975	57			42	1.0
1976		57	42		1.0
1977	42			57	1.0
1979	42	57			1.0
1982	50	50			1.0
1983	50		50		1.0
1985		40	60		1.0
1987	25			75	0.99649
1988	25	75			0.99649
1990		75	25		0.996486
1991			75	25	0.996486
1992	75		25		0.996486
1993		75		25	0.996486

SSRs (tot: 1)

Start position	End position	Sequence	Length
1213	1224	TGC TGC TGC TGC	12

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain