SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene32691

Length: 1707 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene32691 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	serine/threonine-protein kinase AFC1 [Arabidopsis thaliana] gb\|AEE79109.1\| serine/threonine-protein kinase AFC1 [Arabidopsis thaliana]	-	-	70%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	95%
Blast2go	protein	-	-	84%

Source	ECs	Term	Type	e value	Identity
Blast2go	Ferrochelatase.	EC:4.99.1.1	-	0.0	84%

Source	KEGGs	Term	Identity
Blast2go	Porphyrin and chlorophyll metabolism	00860	84%
Blast2go	Metabolic pathways	01100	84%
Blast2go	Biosynthesis of secondary metabolites	01110	84%

Source	GOs	Term	Type	Identity
Blast2go	response to oxidative stress	GO:0006979	Biological Process	84%
Blast2go	heme biosynthetic process	GO:0006783	Biological Process	84%
Blast2go	ferrochelatase activity	GO:0004325	Molecular Function	84%
Blast2go	transferase activity	GO:0016740	Molecular Function	84%
Blast2go	protein binding	GO:0005515	Molecular Function	84%
Blast2go	mitochondrion	GO:0005739	Cellular Component	84%
Blast2go	chloroplast	GO:0009507	Cellular Component	84%

Source	InterPros	Term	Type	Identity
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	Serine/threonine- / dual-specificity protein kinase, catalytic domain	IPR002290	-	-
Sma3	Serine/threonine-protein kinase, active site	IPR008271	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-
Sma3		IPR017442	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LL66

Fln msg: Separated hits, possible frame ERROR between 600 and 753, and possible frame ERROR between 1185 and 1187, your sequence is shorter than subject: 432 - 459

Fln protein: YKILKKIGEGTFGQVLECWDREIRDFVAIKIVRGLQRYREAAFIEIDALREVAKYDRSGTRCVQMLNWFDYRNHICIVFEKLGPSLYDFLRKNNYHPFPIDIVRELGKQLLESVAYMHQLHLIHTDLKPENILFASSEYKKVPYYKNGLKRLSQDGTCYMRLPKSTAIKLIDFGSATFEDQNHSSIISTRHYRAPEVILxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxISGFGWSYPADVWSIGCILVELCVVINPICFFV*IYLLLVKFWSLLSQHGGRNKFAILNSISVTLS*ICRGNVVSNT*KHGASCHDGKGIGAISTTSN*ESRCTISKVFSTWNKIKFSRRSIFKRKHQSCQEVTPTTEFNNGTxx*PLCWVTHRLAARPS*V*AFRAFGSSRSSEASLLH*

Protein Length: 433

Fln nts: TACAAAATACTCAAAAAAATTGGGGAAGGAACTTTTGGTCAGGTTTTGGAATGTTGGGACCGGGAAATAAGAGACTTTGTGGCCATCAAAATTGTTCGTGGTTTACAGAGATATCGTGAAGCAGCATTTATTGAAATTGATGCACTTCGTGAAGTTGCTAAGTATGATAGAAGTGGCACAAGATGCGTTCAGATGCTGAACTGGTTTGACTATCGCAATCATATTTGCATTGTTTTTGAGAAGCTTGGACCGAGTTTATATGATTTTCTAAGGAAAAACAATTATCATCCATTTCCCATTGATATTGTTCGGGAGCTTGGCAAACAACTTTTGGAGTCTGTGGCATATATGCATCAGTTGCATCTCATTCACACAGATTTGAAGCCTGAAAATATACTTTTTGCGTCATCAGAGTACAAGAAGGTTCCATATTATAAGAATGGCTTGAAGAGGCTTTCACAAGATGGAACGTGTTACATGAGATTGCCAAAGTCAACTGCAATAAAGCTTATTGATTTTGGAAGTGCTACTTTCGAGGACCAGAATCATAGTTCTATAATTTCTACAAGGCATTATAGAGCACCTGAAGTAATTCTAGGxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxCATCTCAGGTTTTGGATGGAGCTACCCTGCAGATGTCTGGAGTATTGGATGCATACTTGTGGAGCTTTGTGTGGTAATAAATCCCATATGTTTTTTTGTTTAAATTTATCTCCTTTTGGTTAAGTTTTGGAGTTTGTTAAGTCAACATGGAGGAAGAAATAAATTTGCTATTTTAAATTCTATTAGTGTAACTCTATCGTAAATTTGTAGGGGAAACGTTGTTTCAAACACATGAAAACATGGAGCATCTTGCCATGATGGAAAGGGTATTGGGGCCATTTCCACAACATCTAATTAGGAAAGCAGATGCACGATCAGCAAAGTATTTTCGACATGGAACAAGATTAAATTTTCCCGAAGGAGCATCTTCAAGAGAAAGCATCAGAGCTGTCAAGAAGTTACCCCGACTACGGAATTTAATAATGGAACGTGxxxGTGACCTCTCTGCTGGGTCACTCATAGACTTGCTGCAAGGCCTTCTTAAGTATGAGCCTTCAGAGCGTTTGGAAGCTCAAGAAGCTCTGAGGCATCCCTTCTTCACTGAAGATTATAGGGGATTTGGAAGTGGAGTGTTTAACATATGATGTTCATTAACTTGCAGCCTCCTGCCTTCACCGTCTGTCATACAATTTCTGGAGTTCCCTGTTGCAGCCGACAATTGATTCGTAGTGCCAGGCAAGGTTTGGTCGAATTGATTTTTCGGTCCCGTGATAGGCTTTGCAAGGCCTACGGCTGGCTATTGTAAAATTATTTATATCAAGCACTCTGTAGTCTAAGTGTTGCAAAAACTGCTGCAAAATATTTCTGTATGATTCAATCATTTTTTTTCAAGGAGAGGTTGCCTGTATTATGCAATTGATTAAATCAACGCTAAAATTTTGTGTCATCTGCACTAGTGGTGCCGATAATGCAATAGGGCATCTTAATTTCCAGATTAGAATTGTTGTTTCAAATTTTT

Fln Alignment:

AllPineV4_c17765___YKILKKIGEGTFGQVLECWDREIRDFVAIKIVRGLQRYREAAFIEIDALREVAKYDRSGTRCVQMLNWFDYRNHICIVFEKLGPSLYDFLRKNNYHPFPIDIVRELGKQLLESVAYMHQLHLIHTDLKPENILFASSEYKKVPYYKNGLKRLSQDGTCYMRLPKSTAIKLIDFGSATFEDQNHSSIISTRHYRAPEVILGxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxISGFGWSYPADVWSIGCILVELCVxDLSAGSLIDLLQGLLKYEPSERLEAQEALRHPFFTEDYRGFGSGVFNI
B8LL66_______________YKILNKIGEGTFGQVLECWDRETREFVAVKIVRGLQRYREAAMIEIDVLREVAKYDRSGTRCVQMRNWFDYRNHVCIVFEKLGPSLYDFLRKNNYHPFPIDIVRELGKQLLESVAYMHHLHLIHTDLKPENILFASSEYKKVPYYKNGLKRLSQDGTCYMRLPKSTAIKLIDFGSATFEDQNHSSIISTRHYRAPEVILGxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxILGLGWSYPADVWSIGCILVELCVxDHSAGALIDLLQGLLKYEPSERLEAQEALRHPFFTEDYRGFGSGVFNI

SNPs (tot: 6)

Position	A %	C %	T %	Probability
1541		11	88	0.923564
1551	88	11		0.991528
1553	11		88	0.923564
1623	87	12		0.992554
1625		87	12	0.992554
1626		87	12	0.992554

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain