SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene28798

Length: 2727 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene28798
ATGGGGGCTCAGAGGCCTGTGATCCACAAGCGTCCTTTGGATAAGTTGATAGCGAATCCACTCAATCCTTTGGGCTAAGTTAACATCGACATAAAGAATTTCTGTACACGATGATTTGCTCTGATCCAAAAAAACTGCATACGTGGAACGATGGGAAGAAGTTGCGTTAAGAATACGTACACAGAATTTCCAGTACTTTCTACAACTCCTTGCCCGCACAAATTATCTTTCAGAAATTAAGCTGCAACAATGGATAATTTATCACTAAGCTTTGGTGGCAGACATTTTGGTCCAGTTCTTGTTTGCATTCGTCAGAGTATTTCTTAACAGGGGAGAGACTTTTCAACAAGTCTTGTTGATTCTATAGTTTCTTCTACAAGGGCAGGCTACTCGAGTAACCTTATAAAACTTATATTTGTCTATTGGCTTCTATCTATTTCGTCTGATATGGAAGTTATGAGGACATCACAATGTGTGCCACAATACAACAGGTATGCACTGTTTGTGCAGAGGGACTTCAATGGGTCTTCTGCTAGTAGCAACAGTCATCTTCGCAACTTGTTTTATCAAATATTTGTTGACAACTATCTAGATGTAAAAACAAGTACTTACAGTCAGAAGACAGAAAAGTTGGCACCTACAAGACAAAAAAGAAAGATTCTGGTTTATGCAGCATCCAATTCAAGTTCTAGTTCGCAAGGAGCTGCACCAAGAAATAAGGTACCATTACGGAAAAGAGGAGAAAAAGATAATTCTATGATGAGAGTTTCACAAGAAAAATCTAGCAAAGCTAATGGAGGAGCTCGATCTTGAACGAGGTGTCTGCTTTCCTTTTCGAAAATATACTCCAGAAATGGTTAGGGCAAAAGTTTTAGGCTCAAGAGGGGCAGTATTCTCACTTTTTGGTCGTGGAGTAGAGATCCTCTCGAAACTAGGGCTATATTGGATCACCCTTTTCTATGATCGACTTGTTGGGAGGGGTCTAGAGATAGTTCCATTACGTGCAGAGCAACTTCGGAATTTGCTATGTGATTTGGGACCTTCTTTTATCAAAGCTGGCCAGGTTTTGGCAAACAGACCTGATATTGTTCGTGAAGATTATATGAATGAACTTTGCATTCTCCAAGATGATGTTCCTTCATTTCCAAATAAGATTGCTTTTACAATCATAGAAGAAGAGGCCGGTGTACCTCTTGAACAGATATTTAGCAAAATATCTTCTCAGACTATAGCAGCAGCAAGTTTAGGACAGGTTTATCGTGCCACTCTTCGTGGCTCAGTCGAAGAGGTTGCTATAAAGGTACAGAGGCCAGAAATTGAACCTATCATATACAGGGACCTTTTCCTTTTCGAACTCTAGCTTCATTTCTGAATGGCATTAGTCTTGAGAAGCTTGGTTGTAATGCGGAGCTTATTGTTGATGAATTTGGCGAGAAGCTACTTGAAGAGCTTGACTATACACTTGAGGCACGTAACATTGAGGACTTCTTGGAGAACTTCAAAGATGATCCAACAGTTAAGATTCCTCGAGTATACAAAGAACTTTCTGGTTCACGGGTTCTTGTGATGGAATGGATTGATGGCATCCGTTGCACTGATCCACAAGCTATTAAAGAAGTTGGCATCCGCATACCTGATTTTCTCACTGTTGGTGTGAGTGCTGCTCTGAGACAGCTGCTAGAATTTGGGTTGTTTCATGGAGATCCACATCCTGGGAACATCTTTGCCATGCGTGATGGACGTATTGCATATGTAGACTTTGGAAATGTTGCAGTTCTTAGTCAGCAAAACAAACAAATCCTGATTGATGCAGTGGTACATGCTGTGAATGAGGACTATGCGGAGATGGCAAATGATTTTACAAGACTGGGTTTCCTTGCTCCTGGCACTGACGTAACTCCTATTATTCCAGCATTAGAAGCAATATGGCAGAACTCAGTTGGGAAAGGACTCGCTGATTTTAATTTTAGGATCGTCACTGAGAAATTTAACAAACTTGTATACCAGTATCCAATCCGTATTCCTGAAAGGTTTTCTCTTGTTATTCGATCATTATTGACTCAAGAAGGAATTTGTTTCACTCTTCAACCAGACTTCAAGTTTCTTGAGGTAGCTTACCCATATGTGGCGAAACGTCTCCTGACAGACCCAGATCCAGCATTACGAGAGCGCCTCATTCAGGTGCTTTTTAAAGACGGAGTATTTCAATGGAAACGCTTAGAAAATCTGATTATATTGGCTAAGGAACAGGTAGCCATTATGAGTCGGAATCCAGCACTGCAAGAAGCAAGCAGACAAGATACAGGAAATCGGCGAGTTGAAAGAAAATTAGATCTGACAGACACACTTAAGGATGGTGCTCGAGTGGTAATAGTAGATGCTAGTATTCGGCGTAAACTTCTTTTGGCTTTGACTGAAGATTCTAAATTGCACGTTCAGGAGCTTATGGATATTTACAGATTAGTTGAAGGGGAATTTGATATTCCTTCCTTGGCTTTAGATCTTGTTCAAAGACTCCCAGTTGCTACACGAGATATGATGCTCGCTTGGAGTGAATCTGTCCTTGTAGATCGTTGAGAATGGCTTTCAAATGCTCTTAAAAATTTATTGTCATGCAGGACCTTTTGTAGTTGATTATTTTCATAAATTGTTTGCAGCAATCACCGGGATATTAAAAAATTTTAATCGTTGAGAATTCAATGACAGTAGTAGCTCGCCGCATACATT

Ace file of the UniGene sp_v2.0_unigene28798 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Predicted unusual protein kinase (ISS) [Ostreococcus tauri] emb\|CAL50461.1\| Predicted unusual protein kinase (ISS) [Ostreococcus tauri]	-	-	64%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	31%
Blast2go	uncharacterized aarf domain-containing protein kinase chloroplastic-like	-	-	87%

Source	GOs	Term	Type	e value	Identity
Blast2go	plastoglobule	GO:0010287	Cellular Component	0.0	87%

Source	InterPros	Term	Type	Identity
Sma3	Protein kinase, catalytic domain	IPR000719	-	-
Sma3	ABC-1	IPR004147	-	-
Sma3	Cation/H+ exchanger	IPR006153	-	-
Sma3	Protein kinase, ATP binding site	IPR017441	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LLY2

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 50 aas, putative chimeric sequence, W1: There is no M at the beginning, your sequence is shorter than subject: 419 - 442

Fln protein: TYHIQGPFPFRTLASFLNGISLEKLGCNAELIVDEFGEKLLEELDYTLEARNIEDFLENFKDDPTVKIPRVYKELSGSRVLVMEWIDGIRCTDPQAIKEVGIRIPDFLTVGVSAALRQLLEFGLFHGDPHPGNIFAMRDGRIAYVDFGNVAVLSQQNKQILIDAVVHAVNEDYAEMANDFTRLGFLAPGTDVTPIIPALEAIWQNSVGKGLADFNFRIVTEKFNKLVYQYPIRIPERFSLVIRSLLTQEGICFTLQPDFKFLEVAYPYVAKRLLTDPDPALRERLIQVLFKDGVFQWKRLENLIILAKEQVAIMSRNPALQEASRQDTGNRRVERKLDLTDTLKDGARVVIVDASIRRKLLLALTEDSKLHVQELMDIYRLVEGEFDIPSLALDLVQRLPVATRDMMLAWSESVLVDR*

Protein Length: 420

Fln nts: ATGGGGGCTCAGAGGCCTGTGATCCACAAGCGTCCTTTGGATAAGTTGATAGCGAATCCACTCAATCCTTTGGGCTAAGTTAACATCGACATAAAGAATTTCTGTACACGATGATTTGCTCTGATCCAAAAAAACTGCATACGTGGAACGATGGGAAGAAGTTGCGTTAAGAATACGTACACAGAATTTCCAGTACTTTCTACAACTCCTTGCCCGCACAAATTATCTTTCAGAAATTAAGCTGCAACAATGGATAATTTATCACTAAGCTTTGGTGGCAGACATTTTGGTCCAGTTCTTGTTTGCATTCGTCAGAGTATTTCTTAACAGGGGAGAGACTTTTCAACAAGTCTTGTTGATTCTATAGTTTCTTCTACAAGGGCAGGCTACTCGAGTAACCTTATAAAACTTATATTTGTCTATTGGCTTCTATCTATTTCGTCTGATATGGAAGTTATGAGGACATCACAATGTGTGCCACAATACAACAGGTATGCACTGTTTGTGCAGAGGGACTTCAATGGGTCTTCTGCTAGTAGCAACAGTCATCTTCGCAACTTGTTTTATCAAATATTTGTTGACAACTATCTAGATGTAAAAACAAGTACTTACAGTCAGAAGACAGAAAAGTTGGCACCTACAAGACAAAAAAGAAAGATTCTGGTTTATGCAGCATCCAATTCAAGTTCTAGTTCGCAAGGAGCTGCACCAAGAAATAAGGTACCATTACGGAAAAGAGGAGAAAAAGATAATTCTATGATGAGAGTTTCACAAGAAAAATCTAGCAAAGCTAATGGAGGAGCTCGATCTTGAACGAGGTGTCTGCTTTCCTTTTCGAAAATATACTCCAGAAATGGTTAGGGCAAAAGTTTTAGGCTCAAGAGGGGCAGTATTCTCACTTTTTGGTCGTGGAGTAGAGATCCTCTCGAAACTAGGGCTATATTGGATCACCCTTTTCTATGATCGACTTGTTGGGAGGGGTCTAGAGATAGTTCCATTACGTGCAGAGCAACTTCGGAATTTGCTATGTGATTTGGGACCTTCTTTTATCAAAGCTGGCCAGGTTTTGGCAAACAGACCTGATATTGTTCGTGAAGATTATATGAATGAACTTTGCATTCTCCAAGATGATGTTCCTTCATTTCCAAATAAGATTGCTTTTACAATCATAGAAGAAGAGGCCGGTGTACCTCTTGAACAGATATTTAGCAAAATATCTTCTCAGACTATAGCAGCAGCAAGTTTAGGACAGGTTTATCGTGCCACTCTTCGTGGCTCAGTCGAAGAGGTTGCTATAAAGGTACAGAGGCCAGAAATTGAACCTATCATATACAGGGACCTTTTCCTTTTCGAACTCTAGCTTCATTTCTGAATGGCATTAGTCTTGAGAAGCTTGGTTGTAATGCGGAGCTTATTGTTGATGAATTTGGCGAGAAGCTACTTGAAGAGCTTGACTATACACTTGAGGCACGTAACATTGAGGACTTCTTGGAGAACTTCAAAGATGATCCAACAGTTAAGATTCCTCGAGTATACAAAGAACTTTCTGGTTCACGGGTTCTTGTGATGGAATGGATTGATGGCATCCGTTGCACTGATCCACAAGCTATTAAAGAAGTTGGCATCCGCATACCTGATTTTCTCACTGTTGGTGTGAGTGCTGCTCTGAGACAGCTGCTAGAATTTGGGTTGTTTCATGGAGATCCACATCCTGGGAACATCTTTGCCATGCGTGATGGACGTATTGCATATGTAGACTTTGGAAATGTTGCAGTTCTTAGTCAGCAAAACAAACAAATCCTGATTGATGCAGTGGTACATGCTGTGAATGAGGACTATGCGGAGATGGCAAATGATTTTACAAGACTGGGTTTCCTTGCTCCTGGCACTGACGTAACTCCTATTATTCCAGCATTAGAAGCAATATGGCAGAACTCAGTTGGGAAAGGACTCGCTGATTTTAATTTTAGGATCGTCACTGAGAAATTTAACAAACTTGTATACCAGTATCCAATCCGTATTCCTGAAAGGTTTTCTCTTGTTATTCGATCATTATTGACTCAAGAAGGAATTTGTTTCACTCTTCAACCAGACTTCAAGTTTCTTGAGGTAGCTTACCCATATGTGGCGAAACGTCTCCTGACAGACCCAGATCCAGCATTACGAGAGCGCCTCATTCAGGTGCTTTTTAAAGACGGAGTATTTCAATGGAAACGCTTAGAAAATCTGATTATATTGGCTAAGGAACAGGTAGCCATTATGAGTCGGAATCCAGCACTGCAAGAAGCAAGCAGACAAGATACAGGAAATCGGCGAGTTGAAAGAAAATTAGATCTGACAGACACACTTAAGGATGGTGCTCGAGTGGTAATAGTAGATGCTAGTATTCGGCGTAAACTTCTTTTGGCTTTGACTGAAGATTCTAAATTGCACGTTCAGGAGCTTATGGATATTTACAGATTAGTTGAAGGGGAATTTGATATTCCTTCCTTGGCTTTAGATCTTGTTCAAAGACTCCCAGTTGCTACACGAGATATGATGCTCGCTTGGAGTGAATCTGTCCTTGTAGATCGTTGA___GAATGGCTTTCAAATGCTCTTAAAAATTTATTGTCATGCAGGACCTTTTGTAGTTGATTATTTTCATAAATTGTTTGCAGCAATCACCGGGATATTAAAAAATTTTAATCGTTGAGAATTCAATGACAGTAGTAGCTCGCCGCATACATT

Fln Alignment:

AllPineV4_c6598___LDYTLEARNIEDFLENFKDDPTVKIPRVYKELSGSRVLVMEWIDGIRCTDPQAIKEVGIRIPDFLTVGVSAALRQLLEFGLFHGDPHPGNIFAMRDGRIAYVDFGNVAVLSQQNKQILIDAVVHAVNEDYAEMANDFTRLGFLAPGTDVTPIIPALEAIWQNSVGKGLADFNFRIVTEKFNKLVYQYPIRIPERFSLVIRSLLTQEGICFTLQPDFKFLEVAYPYVAKRLLTDPDPALRERLIQVLFKDGVFQWKRLENLIILAKEQVAIMSRNPALQEASRQDTGNRRVERKLDLT-------------------------DTLKDGARVVIVDASIRRKLLLALTEDSKLHVQELMDIYRLV----EGEFDIPSLALDLVQRLPVATRDMMLAWSESVLV
B8LLY2_______________MDYEAEAQNGLKFRKLFGQLPEVVIPEMYLDISSRRVLIMEWVEGQKLSE---VKDLHL-----VEVGLYCSLTQLLESGFYHADPHPGNLLRTYDGKLAYIDFGMMGEFKQDFRDGLIEACLHLVNREFDALAGDFVTLGLLPPTAQMSDVANALTGVFQNAVTKGIQNISFGDLSGNLGRTMYKFKFQIPSYFSLVIRSLAVLEGLALSSDPNYKVLGSSYPWIARKVLTDSSPQLRSTLQALLYKDGTFRIDRLESLITESLRSTA--NETLSGQEEKTDKQSRLAIKRILKFTLSEKGDFVREILLEELSKGIDALNRSTFQKAASAVISSLPITFSLPLHLVEDE--DIKNLKTLRRLILALSEGLTEEQNHGLEEGNYL-LKEVDVQMTWNDAVVV

SNPs (tot: 45)

Position	A %	C %	G %	T %	Probability
542		25		75	0.996473
882	66		33		1.0
946		25		75	0.996473
1016	75			25	0.996473
1159	88			11	0.991528
1160	11			88	0.991528
1191		12	87		0.992554
1192		12		87	0.992554
1193		12	87		0.992554
1195	87			12	0.992554
1353	75			25	0.996473
1354			75	25	0.996473
1383		33	66		0.997452
1385	66			33	0.997452
1386			66	33	0.997452
1444			25	75	0.996473
1445	75	25			0.996473
1682	80	20			0.995493
1683	80	20			0.995493
1685	80			20	0.995494
1719		80	20		0.995493
1720	80			20	0.995493
1763	20			80	0.995493
1764		80		20	0.995493
1838	25	75			0.996473
1841		75		25	0.996473
1842			75	25	0.996473
1924	33		66		0.997452
1927			83	16	0.994512
1928	83			16	0.994512
2123		20		80	0.995495
2148	20			80	0.995493
2149		20		80	0.995493
2150	20		80		0.995493
2151		20		80	0.995493
2197		25	75		0.996473
2199		25	75		0.996473
2201	75			25	0.996473
2534	83	16			0.994513
2535	83	16			0.994513
2536		16		83	0.994513
2537	16			83	0.994519
2561	75		25		0.996473
2566		25		75	0.996473
2580	50	50			1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain