SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene28760

Length: 1648 nt

Origin of reads: Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v2.0_unigene28760 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Asparagine synthetase n=3 Tax=Pinaceae RepID=E7EAQ2_PINPS	-	-	85%
FL-Next	tr=Asparagine synthetase; Pinus pinaster (Maritime pine).	-	-	90%
Blast2go	asparagine synthetase	-	-	92%

Source	ECs	Term	Type	e value	Identity
Blast2go	Aspartate--ammonia ligase.	EC:6.3.1.1	-	0.0	92%

Source	KEGGs	Term	Identity
Blast2go	Alanine, aspartate and glutamate metabolism	00250	92%
Blast2go	Cyanoamino acid metabolism	00460	92%
Blast2go	Nitrogen metabolism	00910	92%
Blast2go	Metabolic pathways	01100	92%
Blast2go	Biosynthesis of secondary metabolites	01110	92%

Source	GOs	Term	Type	Identity
Blast2go	hyperosmotic salinity response	GO:0042538	Biological Process	92%
Blast2go	asparagine biosynthetic process	GO:0006529	Biological Process	92%
Blast2go	aspartate-ammonia ligase activity	GO:0004071	Molecular Function	92%

Source	InterPros	Term	Type	Identity
Sma3	Glutamine amidotransferase, class-II	IPR000583	-	-
Sma3	Asparagine synthase	IPR001962	-	-
Sma3	Asparagine synthase, glutamine-hydrolyzing	IPR006426	-	-
Sma3	Rossmann-like alpha/beta/alpha sandwich fold	IPR014729	-	-
Sma3	Glutamine amidotransferase, type II	IPR017932	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: E7EAQ2

Fln msg: your sequence is shorter than subject: 493 - 593

Fln protein: VVTLAVTDGSVWFASEMKALHDDCERFELFPPGHIYSSKQGGLRRWYNPTWYSENHIPSTPYDPLILRRAFEKAVIKRLMTDVPFGVLLSGGLDSSLVAAVAARYLAATKAAKQWGEQLHSFCVGLQGSPDLKAAKEVAEYLGTVHHEFHFTVQDGIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPNKEEFHQETCRKIKALHMYDCLRANKSTSAWGAEARVPFLDKEFINVAMAIDPEWKMVKPDIGRIEKWVLRKAFDDEKNPYLPKHILYRQKEQFSDGVGYSWIDGLKSHAAEHVTDTMLVNAKYIFPDNTPTTKEAYYYRMIFERFYPQNAARLTVPGGPSVACSTAKAVEWDAAWAHNLDPSGRAALGVHASAYQECNVGADKVRENGGADIFGHMQIKDVTVAAREVVIKGKFFKTESRSRWPIRGSTVYISIYNHVIRVSRYNRLVYISTHQC*

Protein Length: 494

Fln nts: CGTCGTGACCTTGGCTGTCACTGATGGATCTGTTTGGTTTGCGTCTGAAATGAAAGCTTTGCATGATGATTGTGAACGTTTTGAGTTGTTTCCCCCTGGACATATATACTCCAGCAAACAAGGAGGCCTGCGCAGATGGTACAACCCAACTTGGTATTCAGAAAATCATATACCGTCCACTCCATATGACCCTTTGATTCTGAGACGAGCATTTGAGAAAGCAGTCATAAAAAGGCTGATGACTGATGTTCCATTTGGAGTACTGTTATCAGGAGGGCTCGACTCATCCTTAGTTGCAGCAGTTGCAGCTCGCTATTTGGCTGCTACCAAGGCTGCAAAACAGTGGGGAGAGCAACTACACTCTTTCTGCGTTGGTTTGCAGGGATCGCCAGATCTAAAGGCTGCAAAAGAGGTTGCAGAGTATCTCGGTACCGTTCATCACGAATTCCATTTTACAGTGCAGGATGGTATAGATGCAATCGAGGACGTTATATACCACGTTGAAACATACGACGTGACAACTATCAGAGCAAGCACGCCCATGTTCTTGATGTCTCGGAAAATCAAGTCGCTAGGAGTGAAGATGGTTCTGTCTGGAGAAGGATCAGACGAGATATTTGGGGGCTATTTGTACTTTCACAAAGCTCCTAACAAAGAGGAATTCCACCAGGAAACATGCCGCAAGATAAAGGCTCTTCACATGTATGATTGCTTGAGGGCTAATAAGTCAACATCTGCATGGGGTGCGGAGGCCCGTGTTCCATTTTTAGATAAGGAATTCATCAACGTTGCCATGGCCATTGATCCCGAGTGGAAGATGGTGAAGCCTGATATTGGGCGCATTGAGAAGTGGGTCTTGCGGAAGGCATTCGATGACGAGAAGAATCCTTATCTTCCCAAGCACATTCTGTACAGACAGAAGGAGCAATTCAGCGATGGGGTTGGGTACAGTTGGATTGACGGCTTAAAATCACATGCGGCAGAGCATGTGACAGATACGATGCTGGTCAATGCCAAATATATATTCCCCGACAATACTCCCACTACAAAAGAGGCATACTACTACAGGATGATATTTGAAAGATTTTATCCTCAGAACGCGGCGCGATTGACGGTTCCGGGCGGGCCGAGCGTGGCTTGTAGCACCGCAAAGGCAGTGGAATGGGATGCTGCGTGGGCTCATAATCTCGATCCCTCCGGCAGGGCCGCTCTAGGCGTCCATGCCTCTGCGTACCAAGAGTGTAATGTAGGTGCAGATAAAGTCAGAGAGAATGGCGGCGCCGATATATTCGGCCATATGCAAATAAAGGACGTAACTGTAGCCGCCCGGGAAGTGGTGATAAAAGGTAAGTTTTTTAAAACAGAGAGCAGAAGTCGCTGGCCGATACGGGGTTCCACTGTATACATTAGTATATATAACCATGTAATCCGTGTGTCTCGTTATAATAGACTAGTGTACATCTCTACGCACCAATGCTAA___GTGTACACGTTTGTCAGGTTGTATTTCAATTTGCTGGGTTTGGTTTTCAATATTGATGTCAGAGGACGGGGTGAAATGTGATTTCAATGTTATTATACCAGTGTGGACAAACTTTTTACGTGAATTATATGTTATAATGTGTACTGAAGAGTTTGTTTGGATTTGAAC

Fln Alignment:

AllPineV4_c6377___DGSVWFASEMKALHDDCERFELFPPGHIYSSKQGGLRRWYNPTWYSENHIPSTPYDPLILRRAFEKAVIKRLMTDVPFXXXXXXXXXXXXVXXXXXXXXXXXXXAKQWGEQLHSFCVGLQGSPDLKAAKEVAEYLGTVHHEFHFTVQDGIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPNKEEFHQETCRKIKALHMYDCLRANKSTSAWGAEARVPFLDKEFINVAMAIDPEWKMVKPDIGRIEKWVLRKAFDDEKNPYLPKHILYRQKEQFSDGVGYSWIDGLKSHAAEHVTDTMLVNAKYIFPDNTPTTKEAYYYRMIFERFYPQNAARLTVPGGPSVACSTAKAVEWDAAWAHNLDPSGRAALGVHASAYQECNVGADKVRENGGADIFGHMQIKDVTVAAREVVIKG
E7EAQ2_______________DGSVWFASEMKALHDDCESFESFPPGHIYSSKQGGLRRWYNPPWYSENVIPSSPYDSLVLRRAFEKAVIKRLMTDVPFGVLLSGGLDSSLVAAVASRYLAATKAGKQWGDKLHSFCVGLKGSPDLKAAKEVAEYLGTFHHEFHFTVQDGIDAIEDVIYHVETYDVTTIRASTPMFLMSRKIKSLGVKMVLSGEGSDEIFGGYLYFHKAPNKEEFHQETCRKIKALHMYDCLRANKSTSAWGVEARVPFLDKEFVNVAMDIDPEWKMVKPDIGRIEKWVLRRAFDDEQNPYLPKHILYRQKEQFSDGVGYSWIDGLKSHAAEHVTDTMLRNAKYIFPHNTPTTKEAYYYRMIFERFYPQNAARLTVPGGPSVACSTAKAVEWDAAWAHNLDPSGRAALGVHASAYEECNAGANDGTGNGNVDTFSKMPEREESVGAREVLIKG

SNPs (tot: 47)

Position	A %	C %	G %	T %	Probability
397	50			50	1.0
544		23		76	1.0
547		76		23	1.0
565	72		27		1.0
595		20		80	0.999996
601		25		75	1.0
619	28		57		1.0
621		66	16		0.988813
623	16		83		0.994512
625			83	16	0.994512
626	83		16		0.994512
628		16		83	0.994512
630			16	83	0.994512
631	50	16	33		1.0
632		16		83	0.994512
635	16			83	0.994512
636			16	83	0.994512
637	14		85		0.993533
638	14		71	14	0.984337
639		85		14	0.993533
640	85		14		0.993533
642		14		85	0.993533
644	57	42			1.0
646		85	14		0.993533
663		83	16		0.994512
667			16	83	0.994512
668		16	83		0.994512
670	16			83	0.994512
671	16	83			0.994512
672			16	83	0.994512
674		83		16	0.994512
676	16		83		0.994512
680		16		83	0.994512
716		80		20	0.995493
717		80	20		0.995493
721		80	20		0.995493
722	20		80		0.995493
724	20			80	0.995493
726	80	20			0.995493
727	80	20			0.995493
728	20			80	0.995493
729	20			80	0.995493
731		80	20		0.995493
736			20	80	0.995493
737	20			80	0.995493
1426		66		33	0.997452
1428		33		66	0.997454

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain