SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene28358

Length: 2668 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene28358
ATGGGGGGATTTGTTTCTTTTGCCCTGAAGCTTCGTCTGTGGCACGACGAAGCGAGGAAAGTCTTGCGCTATCCCATTACCCTTACAATTCCCCTCTGATATTCTCCACTTCTCCCTCGCCCCGCCCCGTCCTGGATCCATTTGTACTCACTTTGCCCGCCATTTTCCTTATTTTTCTCCCAATATCTGCAGTTGTTTACGTTTTCCAGCTTCCCGTTTATCTTCTCTTCATAACTGCAAATTGCGCATAGATTTTGTCTTGGGATCAAATCCATCAGCTGCCGTTTTCGGCTCTCAAGTCAGGGATTGAGCCCTAGATACATCATCTACAACTTGCTGATCCGTGAGGCGACGTTGTCTCAGGCTAGCTTTTTTCTAGGGCCTGGTATATGCTTTTAGGGATTAGGGTTTGGGCTATTGAGGGTTTGCGCGTATATCCCATATTCAGGGGTCTGCAGGGTTCGAAGCCTAGACGGTATTTGGGATATCTTCGAGGATGGCAGGAGAACGGCAGTATGGTGTTACAGAGCCCATATCAATGGCAGGACCCACTGAGGCTGATCTTCACAGGACTCGGCAGCTGGAAGAGTTTTTGATGAGCGCGGGCCTTTATGAGAGTCCAGAGGACGGTGCCTTAAGGAAGGAGGTTTTGGCCCAAATTGATAAGATTGCAAAAGCATGGGTCAAGAGGATAACTGATGAAAGAGGGTATGGTGAACAGTTGGTGGAAGAAGCAAATGCGAAAATTTATACATTTGGGTCTTATCGTCTATCGGTACATGGTCCAGGATCAGATCTAGACACGCTATGTGTAGGGCCTTGCTATGTGATTAGGGAGGAGGATTTTTTTTGGCGTTTTGCATGATATGTTGGCCGAAATGCCGGAGGTCTCTGAACTCCATCCAGTCCCTGATGCACATGTGCCTGTAATGAAGTTTAAGTTCAGAGGGATATCTATTGATTTGATCTATGCTAGCATATCACTTTGGGTTGTTCCTGAAGATTTTGATATCTCTCAGGAGTCAGTACTTTGTGATGTTGATGAACAAAGTGCAAGGAGCCTCAATGGATGCAGAGTTGCAGATCAGATCTTGCGCCTTGTACCCAATGTTGACCATTTTCGTACAACACTCAGATGCATGAAGCTCTGGGCTAAAAGGCGCGGTCTCTACTCAAATGTCACGGGTTTTTTGGGTGGTGTGAATTGGGCTTTATTAGTTGCTCGGATTTGTCAACTGTATCCAAATGCAATTCCCAGTATGTTGGTTTCGCGATTCTTTAGAGTCTATACACAATGGCGATGGCCAAATCCTGTGATGCTTTGTTCGATAGAGGAGGGTACCCTGGGATTTCCTGTATGGGATCCACGAAAGAACCCTCGTGATCGGACACATCATATGCCCATTATAACTCCTGCTTATCCGTGCATGAACTCCAGTTATAATGTATCTACGAGTACACTTCGTGTCATGACAGAGCAATTTCAGACTGGAAACAAGATTTGTGAGGATATTGAGATGAACAAGGCAGGCTGGGATGCTCTTTTTGAGTCTTATCCTTTCTTTGAGTCATACAGAAATTATTTGCAAATAGACATCGTAGCTGCAGATCAAGATGATTTGAGGACGTGGAAAGGATGGGTTGAGTCACGATTAAGGCAACTTACTTTGAAGATTGAACGAGACACATTTGGGATGCTGCAGTGTCATCCTTACCCTTGTGAATATAGCAACACATCTAGTCAAGGATATCATTGTGCATATTTTATGGGTTTACAAAGGAAACAAGGAGTGCCAATACAGGAAGGTCAACAGTTTGACATACGTGGCACTGTAGACGAATTCAGGCACTCAGTGATCATGTATTTTCTTTGGAGACCAGGAATGGACATCTTTGTATCACATGTACGTCGAAAACAAATTCCACCATATGTCTTTCCTGGAGGGGTATTACCTAGTCGACCTGCAAGAGGTGCGAATCAAACAGTAAAGGCTTCTCCAGGGAGTAGTAGTGGAAATTCTGGCGATGAATCTCCAGAACAGTGCAGCAACAAGTCATCTGTGAAACTGAACAAGAGAAAACATGAAACTGCAGGGAACATTGACAAAGGAGACAAAAAACCTGCTATTAGGTCAAATCGTGTGGTAGCTTGTTTAGGAGCAATGGAAGGTAGTCCATGTCGAGGCAATATAGCTGAAGAAGCAGTTTCTCAGAGGGTAACTGATGGTGATGTACAAAGCCGTCTGAAACAAAAAGTTTCTAAGGTCCTGGGTAGTGCTAGAACCATAGACACAGTTTCTGGCAAAACTTCAACAGAAGGATCATTGGGAAATGTCACTGCAATGAGAGCTGAAAATTTCCAAAATGGGCGGAAGGTGGAGGTCTGGCATTAATAAATGGTGTTTTTGTTAATTTTTAAATCTAATTTAGATATTATGCTTTTTCTTCGAATTCTTTGCATATAAAGAAGAAACCCTGTAGCATGACATTTAGATTTGAGGATATCATAATATATTAATTTATTTTGGGGTTTCCATTGCCAAAAGAGTCAACAAGGTAATATTCCTTGCTGTTACTTCTTCTTGACTATGGCTAAATTTAGATGCAACTGTATGATTCTCTAGGGTTTCAATCTTATCTTCCTATTGCTTTTAAGTTTTTGTTTCTT

Ace file of the UniGene sp_v2.0_unigene28358 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Os06g0558700 protein n=5 Tax=Oryza sativa RepID=C7J4C8_ORYSJ	-	-	64%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	70%
Blast2go	nuclear poly polymerase	-	-	79%

Source	GOs	Term	Type	Identity
Blast2go	RNA polyadenylation	GO:0043631	Biological Process	79%
Blast2go	protein binding	GO:0005515	Molecular Function	79%
Blast2go	nucleus	GO:0005634	Cellular Component	79%

Source	InterPros	Term	Type	Identity
Sma3	Nucleotidyl transferase domain	IPR002934	-	-
Sma3	Sugar transporter, conserved site	IPR005829	-	-
Sma3	Protein of unknown function DUF599	IPR006747	-	-
Sma3	Poly(A) polymerase, RNA-binding domain	IPR007010	-	-
Sma3	Poly(A) polymerase, central domain	IPR007012	-	-
Sma3	Inorganic pyrophosphatase	IPR008162	-	-
Sma3	Poly(A) polymerase	IPR014492	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: C0PQ25

Fln msg: Overlapping hits, possible frame ERROR between 850 and 846, query STOP codon too far from subject stop. Distance to subject end: 49 aas, putative chimeric sequence, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 633 - 701

Fln protein: MAGERQYGVTEPISMAGPTEADLHRTRQLEEFLMSAGLYESPEDGALRKEVLAQIDKIAKAWVKRITDERGYGEQLVEEANAKIYTFGSYRLSVHGPGSDLDTLCVGPCYVIREEDxxxGVLHDMLAEMPEVSELHPVPDAHVPVMKFKFRGISIDLIYASISLWVVPEDFDISQESVLCDVDEQSARSLNGCRVADQILRLVPNVDHFRTTLRCMKLWAKRRGLYSNVTGFLGGVNWALLVARICQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCSIEEGTLGFPVWDPRKNPRDRTHHMPIITPAYPCMNSSYNVSTSTLRVMTEQFQTGNKICEDIEMNKAGWDALFESYPFFESYRNYLQIDIVAADQDDLRTWKGWVESRLRQLTLKIERDTFGMLQCHPYPCEYSNTSSQGYHCAYFMGLQRKQGVPIQEGQQFDIRGTVDEFRHSVIMYFLWRPGMDIFVSHVRRKQIPPYVFPGGVLPSRPARGANQTVKASPGSSSGNSGDESPEQCSNKSSVKLNKRKHETAGNIDKGDKKPAIRSNRVVACLGAMEGSPCRGNIAEEAVSQRVTDGDVQSRLKQKVSKVLGSARTIDTVSGKTSTEGSLGNVTAMRAENFQNGRKVEVWH*

Protein Length: 634

Fln nts: ATGGGGGGATTTGTTTCTTTTGCCCTGAAGCTTCGTCTGTGGCACGACGAAGCGAGGAAAGTCTTGCGCTATCCCATTACCCTTACAATTCCCCTCTGATATTCTCCACTTCTCCCTCGCCCCGCCCCGTCCTGGATCCATTTGTACTCACTTTGCCCGCCATTTTCCTTATTTTTCTCCCAATATCTGCAGTTGTTTACGTTTTCCAGCTTCCCGTTTATCTTCTCTTCATAACTGCAAATTGCGCATAGATTTTGTCTTGGGATCAAATCCATCAGCTGCCGTTTTCGGCTCTCAAGTCAGGGATTGAGCCCTAGATACATCATCTACAACTTGCTGATCCGTGAGGCGACGTTGTCTCAGGCTAGCTTTTTTCTAGGGCCTGGTATATGCTTTTAGGGATTAGGGTTTGGGCTATTGAGGGTTTGCGCGTATATCCCATATTCAGGGGTCTGCAGGGTTCGAAGCCTAGACGGTATTTGGGATATCTTCGAGG_-_ATGGCAGGAGAACGGCAGTATGGTGTTACAGAGCCCATATCAATGGCAGGACCCACTGAGGCTGATCTTCACAGGACTCGGCAGCTGGAAGAGTTTTTGATGAGCGCGGGCCTTTATGAGAGTCCAGAGGACGGTGCCTTAAGGAAGGAGGTTTTGGCCCAAATTGATAAGATTGCAAAAGCATGGGTCAAGAGGATAACTGATGAAAGAGGGTATGGTGAACAGTTGGTGGAAGAAGCAAATGCGAAAATTTATACATTTGGGTCTTATCGTCTATCGGTACATGGTCCAGGATCAGATCTAGACACGCTATGTGTAGGGCCTTGCTATGTGATTAGGGAGGAGGATxxxxxxxxxGGCGTTTTGCATGATATGTTGGCCGAAATGCCGGAGGTCTCTGAACTCCATCCAGTCCCTGATGCACATGTGCCTGTAATGAAGTTTAAGTTCAGAGGGATATCTATTGATTTGATCTATGCTAGCATATCACTTTGGGTTGTTCCTGAAGATTTTGATATCTCTCAGGAGTCAGTACTTTGTGATGTTGATGAACAAAGTGCAAGGAGCCTCAATGGATGCAGAGTTGCAGATCAGATCTTGCGCCTTGTACCCAATGTTGACCATTTTCGTACAACACTCAGATGCATGAAGCTCTGGGCTAAAAGGCGCGGTCTCTACTCAAATGTCACGGGTTTTTTGGGTGGTGTGAATTGGGCTTTATTAGTTGCTCGGATTTGTCAACTGTATCCAAATGCAATTCCCAGTATGTTGGTTTCGCGATTCTTTAGAGTCTATACACAATGGCGATGGCCAAATCCTGTGATGCTTTGTTCGATAGAGGAGGGTACCCTGGGATTTCCTGTATGGGATCCACGAAAGAACCCTCGTGATCGGACACATCATATGCCCATTATAACTCCTGCTTATCCGTGCATGAACTCCAGTTATAATGTATCTACGAGTACACTTCGTGTCATGACAGAGCAATTTCAGACTGGAAACAAGATTTGTGAGGATATTGAGATGAACAAGGCAGGCTGGGATGCTCTTTTTGAGTCTTATCCTTTCTTTGAGTCATACAGAAATTATTTGCAAATAGACATCGTAGCTGCAGATCAAGATGATTTGAGGACGTGGAAAGGATGGGTTGAGTCACGATTAAGGCAACTTACTTTGAAGATTGAACGAGACACATTTGGGATGCTGCAGTGTCATCCTTACCCTTGTGAATATAGCAACACATCTAGTCAAGGATATCATTGTGCATATTTTATGGGTTTACAAAGGAAACAAGGAGTGCCAATACAGGAAGGTCAACAGTTTGACATACGTGGCACTGTAGACGAATTCAGGCACTCAGTGATCATGTATTTTCTTTGGAGACCAGGAATGGACATCTTTGTATCACATGTACGTCGAAAACAAATTCCACCATATGTCTTTCCTGGAGGGGTATTACCTAGTCGACCTGCAAGAGGTGCGAATCAAACAGTAAAGGCTTCTCCAGGGAGTAGTAGTGGAAATTCTGGCGATGAATCTCCAGAACAGTGCAGCAACAAGTCATCTGTGAAACTGAACAAGAGAAAACATGAAACTGCAGGGAACATTGACAAAGGAGACAAAAAACCTGCTATTAGGTCAAATCGTGTGGTAGCTTGTTTAGGAGCAATGGAAGGTAGTCCATGTCGAGGCAATATAGCTGAAGAAGCAGTTTCTCAGAGGGTAACTGATGGTGATGTACAAAGCCGTCTGAAACAAAAAGTTTCTAAGGTCCTGGGTAGTGCTAGAACCATAGACACAGTTTCTGGCAAAACTTCAACAGAAGGATCATTGGGAAATGTCACTGCAATGAGAGCTGAAAATTTCCAAAATGGGCGGAAGGTGGAGGTCTGGCATTAA___TAAATGGTGTTTTTGTTAATTTTTAAATCTAATTTAGATATTATGCTTTTTCTTCGAATTCTTTGCATATAAAGAAGAAACCCTGTAGCATGACATTTAGATTTGAGGATATCATAATATATTAATTTATTTTGGGGTTTCCATTGCCAAAAGAGTCAACAAGGTAATATTCCTTGCTGTTACTTCTTCTTGACTATGGCTAAATTTAGATGCAACTGTATGATTCTCTAGGGTTTCAATCTTATCTTCCTATTGCTTTTAAGTTTTTGTTTCTT

Fln Alignment:

AllPineV4_c2859___RQYGVTEPISMAGPTEADLHRTRQLEEFLMSAGLYESPEDGALRKEVLAQIDKIAKAWVKRITDERGYGEQLVEEANAKIYTFGSYRLSVHGPGSDLDTLCVGPCYVIREEDxxGVLHDMLAEMPEVSELHPVPDAHVPVMKFKFRGISIDLIYASISLWVVPEDFDISQESVLCDVDEQSARSLNGCRVADQILRLVPNVDHFRTTLRCMKLWAKRRGLYSNVTGFLGGVNWALLVARICQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCSIEEGTLGFPVWDPRKNPRDRTHHMPIITPAYPCMNSSYNVSTSTLRVMTEQFQTGNKICEDIEMNKAGWDALFESYPFFESYRNYLQIDIVAADQDDLRTWKGWVESRLRQLTLKIERDTFGMLQCHPYPCEYSNTSSQGYHCAYFMGLQRKQGVPIQEGQQFDIRGTVDEFRHSVIMYFLWRPGMDIFVSHVRRKQIPPYVFPGGVLPSRPARGANQTVK-------ASPGSSSGNSGDESPEQCSNKSSVKLNKRKHETAGNIDKGDKKPAIRSNRVVACLGAMEGSPCRGNIAEEAVSQRVTD
C0PQ25_______________RQYGITKPISLSGPSGSDLQRSRELEEFLVSAGLYESEEESALREEVLAQIDQIVKQWVKQLTREKGYTEQMVEESNAKVFTFGSYRLGVHGPGADIDTLCVGPEYADQEEDxxILLHDMLAEMPEVMELNRVPDAHVPVMKFKFRGISIDLLYASVALRIVPDDLDISQESVLYNVDEQTVRSLNGCRVNEQILRLVPNVEHFRTTLRCMKFWAKRRGVYSNVTGFLGGVNWALLVARVCQLYPNAIPSMLVSRFFRVFTQWRWPNPVMLRSIKEEELGFPVWDPRKNPRDRTHHMPIITPAYPGMNSSYNVSTSTLRVMTEQFQQGNKMCEDIEMNKANWSALFDPYPFFEAYKNYLQVDIVAADQDDLRLWKGWVESRLRQLTLKIERDTFGMLQCHPYPTEYVDNSKQCPHCAFFMGLQRKQGVLIQEGQQFDIRGTVDEFRQSVGMYMFWKPGMDIYVSHVRRKQIPSYVFPEGVRRVRSGRAANQIGKTSPQGRESSPKCCNGNFGDDIVEECNANSAEKANKRKHDMTTDVGKAEKRAAVSPNDVKSASLSVSDVSNRSGLASQTSSAEEPD

SNPs (tot: 56)

Position	A %	C %	G %	T %	Probability
2241		20		80	0.995493
2244			80	20	0.995493
2275	80			20	0.995493
2276		80	20		0.995493
2277	20	80			0.995493
2278		80	20		0.995493
2280	80		20		0.995493
2282		20		80	0.995493
2283	20	80			0.995493
2284		80	20		0.995493
2286		20		80	0.995493
2289	80		20		0.995493
2290	80		20		0.995493
2291			80	20	0.995493
2295	80	20			0.995493
2296			20	80	0.995493
2299		80	20		0.995493
2331	50		50		0.996687
2335		50	50		0.996687
2341		57		42	1.0
2401		77	22		0.999999
2419	88		11		0.991528
2451	12			87	0.992554
2467	85	14			0.993533
2469		85		14	0.993533
2470			85	14	0.993533
2517			83	16	0.994513
2522	85		14		0.993533
2525	14		85		0.993533
2527	14		85		0.993533
2529		14	85		0.993533
2533	85		14		0.993533
2536		14	85		0.993533
2538		14	85		0.993533
2539			14	85	0.993533
2542	85		14		0.993533
2546			83	16	0.994512
2552	16	83			0.994512
2556		16	83		0.994512
2557	83	16			0.994512
2558	16	83			0.994512
2559	16		83		0.994512
2562			66	33	1.0
2564	16		83		0.994512
2565		16	83		0.994512
2567	16		83		0.994512
2604	66		33		0.997476
2607			66	33	0.997455
2611	66	33			0.997453
2612	33			66	0.997452
2614		33	66		0.997453
2615		33	66		0.997452
2620		66	33		0.997452
2621			66	33	0.997452
2624	66	33			0.997452
2628			33	66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain