SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene28223

Length: 2114 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene28223
CGTCGTGACCTTGGCTGTCACTCATTTTTGCAATTTTTGAACCCTTTTGAAGGTTTGAGCCGATACTGGGCGGATCCTAGGTGGATCCGAATTCGCACACGATCTGGGGATCTGCGGTTCAATATCCCAGGGTGGACGGATCAGTTTGTGGACCAACTCAAGCTCAAGGAAATGGAGTCTGAGAGGTATGAGAAAGAGTTAGCAGTTGATTCCAACAAGGGCATTTCCTCTTTTGAGGACATCTCTCCAGTATTGGCAAAGGAGCTTCTGCAAGATGCACTCGTGTGGGCTTCCTTACATGGCCTTGTGGTTGGCGATAGGGCTGTACAGGGTTCTGGACAAATCCCGGGTGTTGGGTTGGTGCATGCTCCGTTTGCTCTCTTGCCAACACCTTTTCCAGAAGGCTGTTGGAAACAGGCCTGTGATTTGGCACCCTTATTTAATGAGCTTGTAGATCGAGTCAGCATGGACGGAGTTTTTCTGCAAGAGTCACTTTCCAGGACAAAGACAGCTGATGCCTTCACTGCTCGACTCTTAGATATCCATTCTAGTATAATGAAAGATAACATAAAACAGGAAATACGCTTGGGTCTTCATCGTTCAGATTATATGCTTGATATAGCTACAGGAAAACTGCTACAAGTAGAGCTTAATACAATATCATCGTCTTTTGCAGGACTTGGTTGCCTTGTTAGTCAGCTTCACAGATACTTGATCAATCATTCTGGGAAGAAACTTAGCTTGGATTCAAAAAGAGTACCTGAAAACACAGCAGCAAACAAATTTGCAGAGGCATTGGCCCTAGCCTGGAAGGAGTTCAATGACTTAAGTTCTGTAGTACTGGTAGTTGTGCAGCCAGAGGAGCGTAATATGTATGATCAACACTGGATTTGCATGGTGCTGAAAGAATCATATGCGGTGCCATCTATCCGGAAAACTCTAGCTGAAATTGATGCTGAGGGTGAACTTTTATCAAATGGAACTCTTCACATAGGACAACGAGCTGTTGCTGTTGTTTACTTCAGGGCTGGATATACACCTGTTGATTATCCCTCTGAAGCAGAGTGGAGAGCTAGACTAATGTTGGAGCAGTCGTCTGCCATCAAATGCCCTTCAATTTCATATCATTTAGCAGGCACAAAAAAAATTCAACAAGAATTGGCTAAACCTCATGTTCTGGAAAGGTTCATCGATAATAAAGATGACGTTTCTAATCTTAGGAAATGCTTTGCTGGATTATGGAGCTTGGACAATAATGCAAATGTTATAGAAGATGCCATCAGAAGGCCAGAATCATTTGTGCTGAAGCCCCAGCGTGAAGGAGGGGGAAATAACATATATGGAAATGATGTCAAGGAAAAATTACTTGAGTTGAAAAGAAATGGTGGTGATGGACATGCTGCTTACATACTAATGCAAAGGATCTTTCCTCCAATTCATGTGTCATATCTTGCCCGTGGTGGTATCTATCAACACGGTGAAACTGTGTCTGAACTTGGCATATTTGGTGCATATTTACGGAGTAAAGACAATGTGATACTAAACAACCAAAGTGGTTATCTCATGAGAACAAAAGTTTCTTCCTCCAATGAGGGTGGTGTTGCTGCAGGCTTTGCTGTTGTGGATAGTCTCTATCTAATTTGAGTTATCTTCAATTGTACCCTGTGAATTTATGGCATGGAAATTTAAGGAACCTTTTCATTGTCTAATTGGTATTGATTTTTCCATTTCAGTTCTCTTGGAAATGAGAATGTCAACTCCGTTACTTTGCTCGTAGCATGTTCACCTATTGGTTGTCCCACCTCCTGAGAGAATATTCAAATGAAAATAGAATGTGTGCTGGCCTAGTTATAAACTGGTAAAGCTTAGCAAAATTTAGAAGTGGTTCTGTAGCTTGAAGGGTGAAATGTATTGCGTCCTCTTTGAGTAATTGGCCTTTTTAACATGAACATGAATTTATTACATATATTCATGCATAAACTATTCCTTTTCAAAAGTTATACCTCAGACTTCTTCCTTGTACAAGAAGTCTCAATGCTTTGAATCTGTAGAGGCATACAACTATAATCTAGGAATATGCAGCTCTTCTATCCCAACTTAGTTCTTATAGTTAC

Ace file of the UniGene sp_v2.0_unigene28223 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Glutathione synthetase, chloroplastic n=2 Tax=Brassica RepID=GSHB_BRAJU	-	-	59%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	92%
Blast2go	glutathione synthetase	-	-	80%

Source	ECs	Term	Type	e value	Identity
Blast2go	Glutathione synthase.	EC:6.3.2.3	-	0.0	80%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Glutathione metabolism	00480		0.0	80%
Blast2go	Metabolic pathways	01100		0.0	80%

Source	GOs	Term	Type	Identity
Blast2go	response to jasmonic acid stimulus	GO:0009753	Biological Process	80%
Blast2go	glutathione biosynthetic process	GO:0006750	Biological Process	80%
Blast2go	binding	GO:0005488	Molecular Function	80%
Blast2go	glutathione synthase activity	GO:0004363	Molecular Function	80%
Blast2go	cytosol	GO:0005829	Cellular Component	80%
Blast2go	chloroplast	GO:0009507	Cellular Component	80%

Source	InterPros	Term	Type	Identity
Sma3	Peptidase M14, carboxypeptidase A	IPR000834	-	-
Sma3	F-box domain, cyclin-like	IPR001810	-	-
Sma3	Glutathione synthase, substrate-binding, eukaryotic	IPR004887	-	-
Sma3	Glutathione synthase, eukaryotic	IPR005615	-	-
Sma3	ATP-grasp fold, subdomain 2	IPR013816	-	-
Sma3	Glutathione synthase, alpha-helical, eukaryotic	IPR014042	-	-
Sma3	Glutathione synthase, C-terminal, eukaryotic	IPR014709	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5A8U7

Fln msg: your sequence is longer than subject: 491 - 187

Fln protein: MESERYEKELAVDSNKGISSFEDISPVLAKELLQDALVWASLHGLVVGDRAVQGSGQIPGVGLVHAPFALLPTPFPEGCWKQACDLAPLFNELVDRVSMDGVFLQESLSRTKTADAFTARLLDIHSSIMKDNIKQEIRLGLHRSDYMLDIATGKLLQVELNTISSSFAGLGCLVSQLHRYLINHSGKKLSLDSKRVPENTAANKFAEALALAWKEFNDLSSVVLVVVQPEERNMYDQHWICMVLKESYAVPSIRKTLAEIDAEGELLSNGTLHIGQRAVAVVYFRAGYTPVDYPSEAEWRARLMLEQSSAIKCPSISYHLAGTKKIQQELAKPHVLERFIDNKDDVSNLRKCFAGLWSLDNNANVIEDAIRRPESFVLKPQREGGGNNIYGNDVKEKLLELKRNGGDGHAAYILMQRIFPPIHVSYLARGGIYQHGETVSELGIFGAYLRSKDNVILNNQSGYLMRTKVSSSNEGGVAAGFAVVDSLYLI*

Protein Length: 492

Fln nts: CGTCGTGACCTTGGCTGTCACTCATTTTTGCAATTTTTGAACCCTTTTGAAGGTTTGAGCCGATACTGGGCGGATCCTAGGTGGATCCGAATTCGCACACGATCTGGGGATCTGCGGTTCAATATCCCAGGGTGGACGGATCAGTTTGTGGACCAACTCAAGCTCAAGGAA_-_ATGGAGTCTGAGAGGTATGAGAAAGAGTTAGCAGTTGATTCCAACAAGGGCATTTCCTCTTTTGAGGACATCTCTCCAGTATTGGCAAAGGAGCTTCTGCAAGATGCACTCGTGTGGGCTTCCTTACATGGCCTTGTGGTTGGCGATAGGGCTGTACAGGGTTCTGGACAAATCCCGGGTGTTGGGTTGGTGCATGCTCCGTTTGCTCTCTTGCCAACACCTTTTCCAGAAGGCTGTTGGAAACAGGCCTGTGATTTGGCACCCTTATTTAATGAGCTTGTAGATCGAGTCAGCATGGACGGAGTTTTTCTGCAAGAGTCACTTTCCAGGACAAAGACAGCTGATGCCTTCACTGCTCGACTCTTAGATATCCATTCTAGTATAATGAAAGATAACATAAAACAGGAAATACGCTTGGGTCTTCATCGTTCAGATTATATGCTTGATATAGCTACAGGAAAACTGCTACAAGTAGAGCTTAATACAATATCATCGTCTTTTGCAGGACTTGGTTGCCTTGTTAGTCAGCTTCACAGATACTTGATCAATCATTCTGGGAAGAAACTTAGCTTGGATTCAAAAAGAGTACCTGAAAACACAGCAGCAAACAAATTTGCAGAGGCATTGGCCCTAGCCTGGAAGGAGTTCAATGACTTAAGTTCTGTAGTACTGGTAGTTGTGCAGCCAGAGGAGCGTAATATGTATGATCAACACTGGATTTGCATGGTGCTGAAAGAATCATATGCGGTGCCATCTATCCGGAAAACTCTAGCTGAAATTGATGCTGAGGGTGAACTTTTATCAAATGGAACTCTTCACATAGGACAACGAGCTGTTGCTGTTGTTTACTTCAGGGCTGGATATACACCTGTTGATTATCCCTCTGAAGCAGAGTGGAGAGCTAGACTAATGTTGGAGCAGTCGTCTGCCATCAAATGCCCTTCAATTTCATATCATTTAGCAGGCACAAAAAAAATTCAACAAGAATTGGCTAAACCTCATGTTCTGGAAAGGTTCATCGATAATAAAGATGACGTTTCTAATCTTAGGAAATGCTTTGCTGGATTATGGAGCTTGGACAATAATGCAAATGTTATAGAAGATGCCATCAGAAGGCCAGAATCATTTGTGCTGAAGCCCCAGCGTGAAGGAGGGGGAAATAACATATATGGAAATGATGTCAAGGAAAAATTACTTGAGTTGAAAAGAAATGGTGGTGATGGACATGCTGCTTACATACTAATGCAAAGGATCTTTCCTCCAATTCATGTGTCATATCTTGCCCGTGGTGGTATCTATCAACACGGTGAAACTGTGTCTGAACTTGGCATATTTGGTGCATATTTACGGAGTAAAGACAATGTGATACTAAACAACCAAAGTGGTTATCTCATGAGAACAAAAGTTTCTTCCTCCAATGAGGGTGGTGTTGCTGCAGGCTTTGCTGTTGTGGATAGTCTCTATCTAATTTGA___GTTATCTTCAATTGTACCCTGTGAATTTATGGCATGGAAATTTAAGGAACCTTTTCATTGTCTAATTGGTATTGATTTTTCCATTTCAGTTCTCTTGGAAATGAGAATGTCAACTCCGTTACTTTGCTCGTAGCATGTTCACCTATTGGTTGTCCCACCTCCTGAGAGAATATTCAAATGAAAATAGAATGTGTGCTGGCCTAGTTATAAACTGGTAAAGCTTAGCAAAATTTAGAAGTGGTTCTGTAGCTTGAAGGGTGAAATGTATTGCGTCCTCTTTGAGTAATTGGCCTTTTTAACATGAACATGAATTTATTACATATATTCATGCATAAACTATTCCTTTTCAAAAGTTATACCTCAGACTTCTTCCTTGTACAAGAAGTCTCAATGCTTTGAATCTGTAGAGGCATACAACTATAATCTAGGAATATGCAGCTCTTCTATCCCAACTTAGTTCTTATAGTTAC

Fln Alignment:

AllPineV4_c219___MLEQSSAIKCPSISYHLAGTKKIQQELAKPHVLERFIDNKDDVSNLRKCFAGLWSLDNNANVIEDAIRRPESFVLKPQREGGGNNIYGNDVKEKLLELKRNGGDGHAAYILMQRIFPPIHVSYLARGGIYQHGETVSELGIFGAYLRSKDNVILNNQSGYLMRTKVSSSNEGGVAAGFAVVDSLYL
D5A8U7_______________MLEQSSATKCPSISYHLAGTKKIQQELAKPHVLERFIDNKDDIANLRKCFAGLWSLDNNANVIEDAIRRPEAFVLKPQREGGGNNIYGNDAKEKLLEMKRNGGDGHAAYILMQRIFPPFHVSYLVRGGIYHHGETFSELGIFGAYLRNKDNVLLNNQSGYLMRTKISSSDEGGVAAGFAVVDSLYL

SNPs (tot: 30)

Position	A %	C %	G %	T %	Probability
1326	14		85		0.993533
1473	85		14		0.993533
1475			16	83	0.994512
1476	16		83		0.994512
1477		16		83	0.994512
1480		16		83	0.994512
1481		16		83	0.994512
1485		83	16		0.994512
1492	16			83	0.994512
1493		16		83	0.994512
1811		75		25	1.0
1820		87		12	0.992554
1870	87		12		0.992554
1899	37			12	1.0
1900		12	37	50	1.0
1901		62		37	1.0
1902		50	37	12	1.0
1903	50			50	1.0
1906	62		37		1.0
1907	62	37			1.0
1908	57	42			1.0
1909	42		57		1.0
1913	57			42	1.0
1914	57	42			1.0
1916		42		57	1.0
1918	42	57			1.0
1920		57	42		1.0
2055	40	60			1.0
2061		20		80	0.995494
2063			80	20	0.995499

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain