SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene28185

Length: 2785 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene28185
CTACCACAGCGTTCACATTCCACAAAATCACAGGCTGAATTAGTTAAGGCACCTATTTTACAGAGGCACAGCGAAAGCCCTTCTTTGGCTTGGCTGTCACTCCAGTAGAGGACTGGCAGACATGAATATGACCTCAATGACTTCCAAAGATCAAGTTAGAGGTGCCCGCTTACATCAACAGTCTGCATCTATTACACCCAATTTGTATGGTGCCACATCAGCTGCTGGGATGTCCAATGGGGATTGCTCCAACGGGCAATATCAGAGTCCACATATTTCAAATGTATGTACTCCTAATTTTGGCTTGAGTGGATATCCAATTAATCCAATGATTTTACAACCCATGATGGCCAGCTTTGCAGCACCTGGTACTTTGGCTCCCACATTTGACAATATTGCCGCAGCTGCTGGCATGGATTCAAGATCTACTCCTGGTAGCCTGTCTGGAGGAACATCAGGAGGCAACATGGACCTACAGAACTTCTATAGATTAAGTGGGCAAATGGGAGCTGGAATTCAGATGCCAATCATGGATCCAATAAGCATGCAGCAATTGCAGAGAACTGCAGAATATGCTGCACGAATGGCCACAGGATTGAATGATCATTCTCTATCAAGGAATTACATGGGTGGCTCTTATCTTGATTTTCTTGAATTGCAGAAAGCCTACTTGACTGCTTTGCTTGCTCAACAGCAGTCGCAATATGGTGTGCCATATTTGGGAAAAGGTGGCAATGCTAGTCCAGGATGCTATGTCAGTCCTTCCTTTGGCTTTGCCATGCCATATCCTGGTAGCCCAATTGGAAGTCCTGTTCTTCCTGGTTCTCCTATGGCATCTACATCTTCACCTATTCGACATAATGAACGAAACCCTCGTTATTCCTCTGGTTCAAAGAGTTCATCTGTGGGAGGTTGGCAGTCTGAAAATGGGGGTGACATAGAGGAGCGCTATGGTTCCTCTTTGCTGGAAGAGTTCAAGAACAACAAAAACCGATGTTTTGAATTGTCAGATATTGCTGGACATGTTTTTGAGTTCAGCGCTGATCAAATTGGAAGCCGTTTTATTCAACAAAAACTGGAAACTGCAAACTCTGAAGACAAGAATATGGTGTTTATGGAAATCTTTCCTCAAGCATTGATTCTCATGACAGATGTTTTCGGAAATTATGTTCTACAGAAGTTTTTCGAGCATGGCACTGCAAAGCAAAGGCGTGATCTGGCAAATCTGCTTGCTGGCCATGTTTTAACACTTAGTCTTCAAATGTATGGTTGCCGAGTTATCCAGAAGGCTGTTGAGGTTGTTGATGCTGACCAGCAGTTGAAGCTCGTCCAGGAATTGGATGGACATGTTATGCGCTGTGTGCGAGATCAAAATGGGAACCATGTTATCCAGAAGTGTATTGAATGCATTTCCCAGGATAAAATACATTTCATTATTTCAGCATTTTTTGGCCAAGTTGTTGTATTGTCCTCCCATCCATATGGCTGTCGTGTCATTCAGAGAGTATTGGAGCACTGCAAAGAGGACAAGATACAGAGTGTTATGATGGATGAGATCTTGCAATCTGTCTGCACTCTGGCACAAGACCAATATGGAAATTATGTGATACAGCATGTCCTTGAGCATGGTAAACCGCATGAGCGTGCTGCTGTAATTAAGAAACTAGCTGGACAAATAGTTCAGATGAGTCAACAGAAGTTTGCTTCAAATGTTATAGAAAAGTGTTTACAATTTGGAGGTCCAGCTGAGCGACAGATTCTGATCAGTGAGATGCTGGGCTCAACAGATGAAAATGAACCTTTGCAGGCAATGATGAAAGATCAGTTTGCAAATTATGTTGTTCAAAAGGTTTTGGAAACTTGTGATGACCAACAACGTGATTTCATTCTTTGTCGCATCAAAGTGCATCTAAATGCCTTAAAGAAGTATACATATGGAAAGCATATAGTTGCTCGTGTTGAGAAGCTTGTTGCAGCTGGAGAAAGGCGTATTGGAACTCGTCCAACATATCCTTTGTAAATTCTACAGAGATAACACATAAATGAGAACTTGTATAGTTGAGGTTGGTGTTACATGTTGGCGTTGGTCACATTAAAGTGCTGCTGTACTTTCAATTCTGTCAAGTTACTTCAGAAGGAAAGTTCTAGCTGTTGTATATGGATTAGAAATATTTTTATGTTCAGGCTGTGTTGTATATTCTGTAGCATTTATAGACTGAGGGCAAAATAGGGCATATGTGAGGCCCCTTGTGCAGTGAAGTTCTGTTAGAATTTGTGCATGAAATAAGTACTGTGTAAAGAATGTTTGGCAACATAACTGTACAGATGCAATTTCGATTGCATGTGGTGGAATTTGAATTATGTATTTCGCTTGGTGATGGATGCAAGCAATCCATTTATTGTTTCTGAAGAAATGCCCAGAATTTTGCCTAGAAATGCCCTGAATTCTAGCGAGGTGAAAAATCATGTGATTTGCATTCGTTGATTGATTCGAACTCTTCATTTATTGTAGTGTTACATGGGGACATGCTTTCTAAGATGGGCATTGCTCCCAAGATGGTGAAATTCCAAGAGACATGTTTTCTGCTGTTCCCAAAATTTCAAATTTTGGCAAAACATGTCTCAGGAAAAGCAGGATGTTCTACATGTGGGGGACACCTTGGCCCCCTTCCTGATATTTGTCTTTCAAAATTGAAAGGAAAAAAACAAAAACCTGAAGCTTAAATCTTTGTTCATTGGTTTATTTAACACTTAACAGTTAGATCATTTATGTTTTCATATCGTC

Ace file of the UniGene sp_v2.0_unigene28185 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Nucleic acid binding NABP [Medicago truncatula]	-	-	59%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	92%
Blast2go	pumilio homolog 2-like	-	-	73%

Source	InterPros	Term	Type	Identity
Sma3	Peptidase M14, carboxypeptidase A	IPR000834	-	-
Sma3	Pumilio RNA-binding repeat	IPR001313	-	-
Sma3	Aminoacyl-tRNA synthetase, class I, conserved site	IPR001412	-	-
Sma3	Phosphopantetheine attachment site	IPR006162	-	-
Sma3	Domain of unknown function DUF632	IPR006867	-	-
Sma3	Domain of unknown function DUF630	IPR006868	-	-
Sma3	HAT dimerisation	IPR008906	-	-
Sma3		IPR011523	-	-
Sma3	Armadillo-like helical	IPR011989	-	-
Sma3	Nucleic acid binding NABP	IPR012940	-	-
Sma3	Amino acid transporter, transmembrane	IPR013057	-	-
Sma3	Tubulin, conserved site	IPR017975	-	-
Sma3	Peptidase S26A, signal peptidase I, serine active site	IPR019756	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5AD88

Fln msg: your sequence is longer than subject: 633 - 82

Fln protein: MNMTSMTSKDQVRGARLHQQSASITPNLYGATSAAGMSNGDCSNGQYQSPHISNVCTPNFGLSGYPINPMILQPMMASFAAPGTLAPTFDNIAAAAGMDSRSTPGSLSGGTSGGNMDLQNFYRLSGQMGAGIQMPIMDPISMQQLQRTAEYAARMATGLNDHSLSRNYMGGSYLDFLELQKAYLTALLAQQQSQYGVPYLGKGGNASPGCYVSPSFGFAMPYPGSPIGSPVLPGSPMASTSSPIRHNERNPRYSSGSKSSSVGGWQSENGGDIEERYGSSLLEEFKNNKNRCFELSDIAGHVFEFSADQIGSRFIQQKLETANSEDKNMVFMEIFPQALILMTDVFGNYVLQKFFEHGTAKQRRDLANLLAGHVLTLSLQMYGCRVIQKAVEVVDADQQLKLVQELDGHVMRCVRDQNGNHVIQKCIECISQDKIHFIISAFFGQVVVLSSHPYGCRVIQRVLEHCKEDKIQSVMMDEILQSVCTLAQDQYGNYVIQHVLEHGKPHERAAVIKKLAGQIVQMSQQKFASNVIEKCLQFGGPAERQILISEMLGSTDENEPLQAMMKDQFANYVVQKVLETCDDQQRDFILCRIKVHLNALKKYTYGKHIVARVEKLVAAGERRIGTRPTYPL*

Protein Length: 634

Fln nts: CTACCACAGCGTTCACATTCCACAAAATCACAGGCTGAATTAGTTAAGGCACCTATTTTACAGAGGCACAGCGAAAGCCCTTCTTTGGCTTGGCTGTCACTCCAGTAGAGGACTGGCAGAC_-_ATGAATATGACCTCAATGACTTCCAAAGATCAAGTTAGAGGTGCCCGCTTACATCAACAGTCTGCATCTATTACACCCAATTTGTATGGTGCCACATCAGCTGCTGGGATGTCCAATGGGGATTGCTCCAACGGGCAATATCAGAGTCCACATATTTCAAATGTATGTACTCCTAATTTTGGCTTGAGTGGATATCCAATTAATCCAATGATTTTACAACCCATGATGGCCAGCTTTGCAGCACCTGGTACTTTGGCTCCCACATTTGACAATATTGCCGCAGCTGCTGGCATGGATTCAAGATCTACTCCTGGTAGCCTGTCTGGAGGAACATCAGGAGGCAACATGGACCTACAGAACTTCTATAGATTAAGTGGGCAAATGGGAGCTGGAATTCAGATGCCAATCATGGATCCAATAAGCATGCAGCAATTGCAGAGAACTGCAGAATATGCTGCACGAATGGCCACAGGATTGAATGATCATTCTCTATCAAGGAATTACATGGGTGGCTCTTATCTTGATTTTCTTGAATTGCAGAAAGCCTACTTGACTGCTTTGCTTGCTCAACAGCAGTCGCAATATGGTGTGCCATATTTGGGAAAAGGTGGCAATGCTAGTCCAGGATGCTATGTCAGTCCTTCCTTTGGCTTTGCCATGCCATATCCTGGTAGCCCAATTGGAAGTCCTGTTCTTCCTGGTTCTCCTATGGCATCTACATCTTCACCTATTCGACATAATGAACGAAACCCTCGTTATTCCTCTGGTTCAAAGAGTTCATCTGTGGGAGGTTGGCAGTCTGAAAATGGGGGTGACATAGAGGAGCGCTATGGTTCCTCTTTGCTGGAAGAGTTCAAGAACAACAAAAACCGATGTTTTGAATTGTCAGATATTGCTGGACATGTTTTTGAGTTCAGCGCTGATCAAATTGGAAGCCGTTTTATTCAACAAAAACTGGAAACTGCAAACTCTGAAGACAAGAATATGGTGTTTATGGAAATCTTTCCTCAAGCATTGATTCTCATGACAGATGTTTTCGGAAATTATGTTCTACAGAAGTTTTTCGAGCATGGCACTGCAAAGCAAAGGCGTGATCTGGCAAATCTGCTTGCTGGCCATGTTTTAACACTTAGTCTTCAAATGTATGGTTGCCGAGTTATCCAGAAGGCTGTTGAGGTTGTTGATGCTGACCAGCAGTTGAAGCTCGTCCAGGAATTGGATGGACATGTTATGCGCTGTGTGCGAGATCAAAATGGGAACCATGTTATCCAGAAGTGTATTGAATGCATTTCCCAGGATAAAATACATTTCATTATTTCAGCATTTTTTGGCCAAGTTGTTGTATTGTCCTCCCATCCATATGGCTGTCGTGTCATTCAGAGAGTATTGGAGCACTGCAAAGAGGACAAGATACAGAGTGTTATGATGGATGAGATCTTGCAATCTGTCTGCACTCTGGCACAAGACCAATATGGAAATTATGTGATACAGCATGTCCTTGAGCATGGTAAACCGCATGAGCGTGCTGCTGTAATTAAGAAACTAGCTGGACAAATAGTTCAGATGAGTCAACAGAAGTTTGCTTCAAATGTTATAGAAAAGTGTTTACAATTTGGAGGTCCAGCTGAGCGACAGATTCTGATCAGTGAGATGCTGGGCTCAACAGATGAAAATGAACCTTTGCAGGCAATGATGAAAGATCAGTTTGCAAATTATGTTGTTCAAAAGGTTTTGGAAACTTGTGATGACCAACAACGTGATTTCATTCTTTGTCGCATCAAAGTGCATCTAAATGCCTTAAAGAAGTATACATATGGAAAGCATATAGTTGCTCGTGTTGAGAAGCTTGTTGCAGCTGGAGAAAGGCGTATTGGAACTCGTCCAACATATCCTTTGTAA___ATTCTACAGAGATAACACATAAATGAGAACTTGTATAGTTGAGGTTGGTGTTACATGTTGGCGTTGGTCACATTAAAGTGCTGCTGTACTTTCAATTCTGTCAAGTTACTTCAGAAGGAAAGTTCTAGCTGTTGTATATGGATTAGAAATATTTTTATGTTCAGGCTGTGTTGTATATTCTGTAGCATTTATAGACTGAGGGCAAAATAGGGCATATGTGAGGCCCCTTGTGCAGTGAAGTTCTGTTAGAATTTGTGCATGAAATAAGTACTGTGTAAAGAATGTTTGGCAACATAACTGTACAGATGCAATTTCGATTGCATGTGGTGGAATTTGAATTATGTATTTCGCTTGGTGATGGATGCAAGCAATCCATTTATTGTTTCTGAAGAAATGCCCAGAATTTTGCCTAGAAATGCCCTGAATTCTAGCGAGGTGAAAAATCATGTGATTTGCATTCGTTGATTGATTCGAACTCTTCATTTATTGTAGTGTTACATGGGGACATGCTTTCTAAGATGGGCATTGCTCCCAAGATGGTGAAATTCCAAGAGACATGTTTTCTGCTGTTCCCAAAATTTCAAATTTTGGCAAAACATGTCTCAGGAAAAGCAGGATGTTCTACATGTGGGGGACACCTTGGCCCCCTTCCTGATATTTGTCTTTCAAAATTGAAAGGAAAAAAACAAAAACCTGAAGCTTAAATCTTTGTTCATTGGTTTATTTAACACTTAACAGTTAGATCATTTATGTTTTCATATCGTC

Fln Alignment:

Contig28185___MLGSTDENEPLQAMMKDQFANYVVQKVLETCDDQQRDFILCRIKVHLNALKKYTYGKHIVARVEKLVAAGERRIGTRPTY
D5AD88_______________MLGLTDENEPLQAMMKDQFANYVVQKVLETCDDQQRELILSRIKVHLNALKKYTYGKHIVARVEKLVAAGERRIGTQSTY

SNPs (tot: 43)

Position	A %	C %	G %	T %	Probability
2211	14	85			0.993533
2212	71		28		1.0
2214		85		14	0.993533
2297	83		16		0.994512
2299			16	83	0.994512
2300		83		16	0.994512
2301			16	83	0.994512
2303		16		83	0.994512
2309			16	83	0.994512
2312	16			83	0.994512
2313		16	83		0.994512
2314			16	83	0.994512
2316		16	83		0.994512
2336	14		85		0.993533
2406			12	87	0.992554
2420		85		14	0.993533
2421		85	14		0.993533
2428	14			85	0.993533
2431	85	14			0.993533
2435		85	14		0.993533
2437		14	85		0.993533
2438	14		85		0.993533
2440		85	14		0.993533
2442		14		85	0.993533
2443		85	14		0.993533
2649	10			90	0.90298
2660	88	11			0.923564
2661		11		88	0.923564
2686			71	28	1.0
2687			28	71	1.0
2688	28			71	1.0
2689			28	71	1.0
2693		28		71	1.0
2694	28			71	1.0
2701		71	28		1.0
2702		71		28	1.0
2703	71	28			1.0
2704	28		71		1.0
2705	71	28			1.0
2708	71	28			1.0
2711	20		80		0.995493
2713	20			80	0.995493
2714			20	80	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain