SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene27379

Length: 3247 nt

Origin of reads: Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene27379
CGTCGTGACCTTGGATGGCACTCAGATCTTTTGTGTTGACACGATGCGAACTTTAAGCTAAAGAAAGAAATCAAGGTCTTTGGGGTGACCTGCTGGTACTCTGAAGGAAGGAAGAATGCAGGGGAGTATACTTGCTCTTGGATTTGGAATCGGAGTTGGACCTAGTGACGTCAATCTTTCTTGTGGATGGGGATGGCACTGTCATTGTCAATCGAAGAATTCTGAAAGAACACAGTTTTCTTCAAAACAAGATTCAACATGGCAATCTAATAGTTCCAAATGCTTGCTTAGAAAGGATCTACCTCGGAATTTGCACACAGCGTTGAATGGAAGAGTGATCTCCAAGTTTCCTGCTAGGAGGAGAATTTTCATAGTACATTCTACTCTGAAAAATAACAAGTCAACGATGGATAGAACTATTGAGGATGAACTGGAAAATTTGGCTGTTTTGACTGTGGATCCATCCTTGGCTTCACATAAAGCTCATTTCCAGTACAGATATACAAAGTATTTACAACAAAAGCAAGCAATAGAGAAGTATGAAGGGAGCCTTGAGGACTTTTCTAAAGGTTATTTGAAGTTTGGTTTCAATCGAGAAGGAAATAAAATTGTATATCGGGAGTGGGCACCTGCTGCAAAAGAGGCACAGCTTATTGGGGATTTCAATGCTTGGAATGGATTGGGTCACCAAATGGAAAAGGATAAGTTTGGTGTTTGGACAGTCTACATACCGGATAAAGATGGAAATCCTGCTATCCCACATGGTTCAAATGTTAAAATTCGAATGAAGGTTGCTAATGGAACATGGATTGATAGGATACCAGCCTGGATTAGGTATGCCACTGTGGACCCTAGCAACTTTGGTGCAGCGTACGATGGTGTTTATTGGGATCCCTCACCTTCTGAAAGGTATGAATTCAAATATCCCCGGCCACCAAAGCCTGAGGCGCCACGAATTTATGAAGCTCATGTAGGAATGAGTGGCATAGAACCACGTGTAACATCATATGTGGAGTTTGCGGAAAATGTACTCCCTCGCATAAAAGCAAACAACTACAACACAGTTCAGTTGATGGCTGTGGCAGAGCATTCTTATTATGGATCATTTGGATATCATGTCACAAACTTTTTTGCTCCCAGTAGTCGGTCAGGTACGCCAGAGGACCTGAAATTCTTGATTGACAACGCTCACAGTTTAGGTTTACAGGTGTTAATCGACATGGTTCACAGTCATGCTAGTAACAATGTCACAGATGGTCTCAATGGTTATGATATTGGTCAGAGGACAGAAGAATCGTACTTCCACTCTGGAGAGAGGGGATACCACAAGTTGTGGGATAGTCGGCTATTTAACTATGGAAACTGGGAAGTTCAACGTTTTCTGCTTTCTAATTTACGGTGGTGGCTAGAAGAATTCCAGTTTGATGGTTTCCGTTTTGATGGAGTAACTTCAATGTTATACCATCATCATGGTGTTAATGTAGGATTTACTGGGAATTATCAGGAGTACTTTGGCAAGGCAACTGATGTAGATGCCATAGTGTATCTTATGCTAGCCAATTCACTCATACGCAATATCTATCCTGATGCAACCGTTATTGCGGAAGATGTTTCTGGTATGCCAACTTTAGGTCGGCCTGTTTCTGAAGGTGGACTTGGATTTGATTATCGCCTAGCAATGGCTATTCCAGACAAGTGGATTGAATATTTGAAGCATAAACGAGATGAACAGTGGTTGATGGAAGAAATAGTGCTCACACTGACCAACAGGAGATATACTGAGAAATGTGTTGCATATGCAGAAAGTCATGATCAGGCAATTGTTGGTGACAAGACTATTGCATTCACCTTAATGGACAAGGAGATGTACTCTGCCATGTCATGTTTGCAATCACTTTCTCCAGTTGTTGATCGTGGGATTGCACTTCACAAGATGATTCATTTCATAACAATGGCACTAGGTGGCGAGGGCTATCTTAATTTTATGGGAAATGAGTTTGGTCATCCGGAATGGATTGACTTTCCAAGGGAAGGAAATGGTTGGAGCTATGAAAAATGTAGAAGACAGTGGAACTTAGTGGACACGGATCACCTGAGATACAAGTTTATGAATAATTTTGACAAAGCCATGAATGAGCTTGATGAGAAATTTCATTTCCTCGCATCGTCAAAGCAAATAGTTAGCAGTGCAAATGAAGAAGATAAGGTGATTGTTTTTGAACGAGGCGATATAGTGTTTGTTTTCAACTTCCATCCCAAGAATACATATTCTGGGTACAAGATTGGTTGTGATATGCCTGGAAAATATAGAGTTGCCTTAGACAGTGATGCATTAGAATTTGGTGGTCATGGCAGGGTAAGTCATGATGTGGACCATTTCACTTCCCCTGAAGGTGTACCTGGGAAACCAGAAACAAATTTTAACGGTCGGCCAAATTCTTTCAAAATATTAATGCCTTCACGTACATGCCTGGTATATTTTAAAGTTCCAGAGACTACTCATCTTACTGGTGATAGCACCTTTAGCATGGCTCATCTTACTGGTGATAGCACCTTTAGCATGGTTGGAAAACCTGTACTGGATTGATATGTCTCAGCGTTGCTATGGTTGAAACGTCGGCTTGAAATATAATGCTCAAAAGTAATCACTAATCATCTTCCATTCTGGATTTCTTATAGAAAGTGCAGTCAGGACAGTAGTGAATTAATGCACAGCACTAAGTTTAATGGAATTTCTCTAGGAACCAACTACTTAGTTCATTTTGTTTAACAATTGTATTTGGCACAACGAAAAATTATATTTACTCCATAGCAGTGAACCAGCATGGTTTAAATTCTTTATTTCTTATGTTCTAGAACTTTAGGAGTCTGAAATGAGTAGAGGTGTCTGTACATGCTAGTTTGTATAATAAGACCAATAAATTTTGAAGAAGTTAGTATTATACAATATGCTTTTTTCTAGCTAATGCAAAAGATTGCAGCTTTCACCCATTATCTGCTTGAAAAGTTGAATGTGTCAAGATTTGGTGGAAGGCAGTTTTGTGGCATGCTCTGTGCATTATCTCTGGAATTTGCGTGATGGGCAGTAAACAATTTTCTTGCTTGCAGAAAATACTAAGAATTAGTGCTTAAACAAGATGAACACATTAAAAAGGTGCACACCAGATTTGACTGGCCTGTGTTAATTTTCATTGTTGATGTACATCTGCAGCTCAAAATCTCAATTCTCAACCAATTATGTTCTCAT

Ace file of the UniGene sp_v2.0_unigene27379 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	1,4-alpha-glucan branching enzyme (Fragment) n=1 Tax=Solanum tuberosum RepID=O04864_SOLTU	-	-	76%
FL-Next	sp=1,4-alpha-glucan-branching enzyme; Solanum tuberosum (Potato).	-	-	72%
Blast2go	starch branching enzyme 1	-	-	85%

Source	GOs	Term	Type	e value	Identity
Blast2go	catalytic activity	GO:0003824	Molecular Function	0.0	85%

Source	InterPros	Term	Type	Identity
Sma3	Alpha-amylase, C-terminal all beta	IPR006048	-	-
Sma3	Glycosyl hydrolase, family 13, all-beta	IPR013780	-	-
Sma3	Glycosyl hydrolase, family 13, catalytic domain	IPR006047	-	-
Sma3	Glycoside hydrolase, family 13, N-terminal	IPR004193	-	-
Sma3	Glycoside hydrolase, subgroup, catalytic domain	IPR013781	-	-
Sma3	Immunoglobulin-like fold	IPR013783	-	-

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: sp_plants

Fln subject: P30924

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 57 aas, putative chimeric sequence, your sequence is shorter than subject: 824 - 861

Fln protein: MQGSILALGFGIGVGPSDVNLSCGWGWHCHCQSKNSERTQFSSKQDSTWQSNSSKCLLRKDLPRNLHTALNGRVISKFPARRRIFIVHSTLKNNKSTMDRTIEDELENLAVLTVDPSLASHKAHFQYRYTKYLQQKQAIEKYEGSLEDFSKGYLKFGFNREGNKIVYREWAPAAKEAQLIGDFNAWNGLGHQMEKDKFGVWTVYIPDKDGNPAIPHGSNVKIRMKVANGTWIDRIPAWIRYATVDPSNFGAAYDGVYWDPSPSERYEFKYPRPPKPEAPRIYEAHVGMSGIEPRVTSYVEFAENVLPRIKANNYNTVQLMAVAEHSYYGSFGYHVTNFFAPSSRSGTPEDLKFLIDNAHSLGLQVLIDMVHSHASNNVTDGLNGYDIGQRTEESYFHSGERGYHKLWDSRLFNYGNWEVQRFLLSNLRWWLEEFQFDGFRFDGVTSMLYHHHGVNVGFTGNYQEYFGKATDVDAIVYLMLANSLIRNIYPDATVIAEDVSGMPTLGRPVSEGGLGFDYRLAMAIPDKWIEYLKHKRDEQWLMEEIVLTLTNRRYTEKCVAYAESHDQAIVGDKTIAFTLMDKEMYSAMSCLQSLSPVVDRGIALHKMIHFITMALGGEGYLNFMGNEFGHPEWIDFPREGNGWSYEKCRRQWNLVDTDHLRYKFMNNFDKAMNELDEKFHFLASSKQIVSSANEEDKVIVFERGDIVFVFNFHPKNTYSGYKIGCDMPGKYRVALDSDALEFGGHGRVSHDVDHFTSPEGVPGKPETNFNGRPNSFKILMPSRTCLVYFKVPETTHLTGDSTFSMAHLTGDSTFSMVGKPVLD*

Protein Length: 825

Fln nts: CGTCGTGACCTTGGATGGCACTCAGATCTTTTGTGTTGACACGATGCGAACTTTAAGCTAAAGAAAGAAATCAAGGTCTTTGGGGTGACCTGCTGGTACTCTGAAGGAAGGAAGA_-_ATGCAGGGGAGTATACTTGCTCTTGGATTTGGAATCGGAGTTGGACCTAGTGACGTCAATCTTTCTTGTGGATGGGGATGGCACTGTCATTGTCAATCGAAGAATTCTGAAAGAACACAGTTTTCTTCAAAACAAGATTCAACATGGCAATCTAATAGTTCCAAATGCTTGCTTAGAAAGGATCTACCTCGGAATTTGCACACAGCGTTGAATGGAAGAGTGATCTCCAAGTTTCCTGCTAGGAGGAGAATTTTCATAGTACATTCTACTCTGAAAAATAACAAGTCAACGATGGATAGAACTATTGAGGATGAACTGGAAAATTTGGCTGTTTTGACTGTGGATCCATCCTTGGCTTCACATAAAGCTCATTTCCAGTACAGATATACAAAGTATTTACAACAAAAGCAAGCAATAGAGAAGTATGAAGGGAGCCTTGAGGACTTTTCTAAAGGTTATTTGAAGTTTGGTTTCAATCGAGAAGGAAATAAAATTGTATATCGGGAGTGGGCACCTGCTGCAAAAGAGGCACAGCTTATTGGGGATTTCAATGCTTGGAATGGATTGGGTCACCAAATGGAAAAGGATAAGTTTGGTGTTTGGACAGTCTACATACCGGATAAAGATGGAAATCCTGCTATCCCACATGGTTCAAATGTTAAAATTCGAATGAAGGTTGCTAATGGAACATGGATTGATAGGATACCAGCCTGGATTAGGTATGCCACTGTGGACCCTAGCAACTTTGGTGCAGCGTACGATGGTGTTTATTGGGATCCCTCACCTTCTGAAAGGTATGAATTCAAATATCCCCGGCCACCAAAGCCTGAGGCGCCACGAATTTATGAAGCTCATGTAGGAATGAGTGGCATAGAACCACGTGTAACATCATATGTGGAGTTTGCGGAAAATGTACTCCCTCGCATAAAAGCAAACAACTACAACACAGTTCAGTTGATGGCTGTGGCAGAGCATTCTTATTATGGATCATTTGGATATCATGTCACAAACTTTTTTGCTCCCAGTAGTCGGTCAGGTACGCCAGAGGACCTGAAATTCTTGATTGACAACGCTCACAGTTTAGGTTTACAGGTGTTAATCGACATGGTTCACAGTCATGCTAGTAACAATGTCACAGATGGTCTCAATGGTTATGATATTGGTCAGAGGACAGAAGAATCGTACTTCCACTCTGGAGAGAGGGGATACCACAAGTTGTGGGATAGTCGGCTATTTAACTATGGAAACTGGGAAGTTCAACGTTTTCTGCTTTCTAATTTACGGTGGTGGCTAGAAGAATTCCAGTTTGATGGTTTCCGTTTTGATGGAGTAACTTCAATGTTATACCATCATCATGGTGTTAATGTAGGATTTACTGGGAATTATCAGGAGTACTTTGGCAAGGCAACTGATGTAGATGCCATAGTGTATCTTATGCTAGCCAATTCACTCATACGCAATATCTATCCTGATGCAACCGTTATTGCGGAAGATGTTTCTGGTATGCCAACTTTAGGTCGGCCTGTTTCTGAAGGTGGACTTGGATTTGATTATCGCCTAGCAATGGCTATTCCAGACAAGTGGATTGAATATTTGAAGCATAAACGAGATGAACAGTGGTTGATGGAAGAAATAGTGCTCACACTGACCAACAGGAGATATACTGAGAAATGTGTTGCATATGCAGAAAGTCATGATCAGGCAATTGTTGGTGACAAGACTATTGCATTCACCTTAATGGACAAGGAGATGTACTCTGCCATGTCATGTTTGCAATCACTTTCTCCAGTTGTTGATCGTGGGATTGCACTTCACAAGATGATTCATTTCATAACAATGGCACTAGGTGGCGAGGGCTATCTTAATTTTATGGGAAATGAGTTTGGTCATCCGGAATGGATTGACTTTCCAAGGGAAGGAAATGGTTGGAGCTATGAAAAATGTAGAAGACAGTGGAACTTAGTGGACACGGATCACCTGAGATACAAGTTTATGAATAATTTTGACAAAGCCATGAATGAGCTTGATGAGAAATTTCATTTCCTCGCATCGTCAAAGCAAATAGTTAGCAGTGCAAATGAAGAAGATAAGGTGATTGTTTTTGAACGAGGCGATATAGTGTTTGTTTTCAACTTCCATCCCAAGAATACATATTCTGGGTACAAGATTGGTTGTGATATGCCTGGAAAATATAGAGTTGCCTTAGACAGTGATGCATTAGAATTTGGTGGTCATGGCAGGGTAAGTCATGATGTGGACCATTTCACTTCCCCTGAAGGTGTACCTGGGAAACCAGAAACAAATTTTAACGGTCGGCCAAATTCTTTCAAAATATTAATGCCTTCACGTACATGCCTGGTATATTTTAAAGTTCCAGAGACTACTCATCTTACTGGTGATAGCACCTTTAGCATGGCTCATCTTACTGGTGATAGCACCTTTAGCATGGTTGGAAAACCTGTACTGGATTGA___TATGTCTCAGCGTTGCTATGGTTGAAACGTCGGCTTGAAATATAATGCTCAAAAGTAATCACTAATCATCTTCCATTCTGGATTTCTTATAGAAAGTGCAGTCAGGACAGTAGTGAATTAATGCACAGCACTAAGTTTAATGGAATTTCTCTAGGAACCAACTACTTAGTTCATTTTGTTTAACAATTGTATTTGGCACAACGAAAAATTATATTTACTCCATAGCAGTGAACCAGCATGGTTTAAATTCTTTATTTCTTATGTTCTAGAACTTTAGGAGTCTGAAATGAGTAGAGGTGTCTGTACATGCTAGTTTGTATAATAAGACCAATAAATTTTGAAGAAGTTAGTATTATACAATATGCTTTTTTCTAGCTAATGCAAAAGATTGCAGCTTTCACCCATTATCTGCTTGAAAAGTTGAATGTGTCAAGATTTGGTGGAAGGCAGTTTTGTGGCATGCTCTGTGCATTATCTCTGGAATTTGCGTGATGGGCAGTAAACAATTTTCTTGCTTGCAGAAAATACTAAGAATTAGTGCTTAAACAAGATGAACACATTAAAAAGGTGCACACCAGATTTGACTGGCCTGTGTTAATTTTCATTGTTGATGTACATCTGCAGCTCAAAATCTCAATTCTCAACCAATTATGTTCTCAT

Fln Alignment:

Contig27379___SKNSERTQFSSKQDSTWQSNSSKCLLRKDLPRNLHTALNGRVISKFPARRRIFIVHSTLKNNKSTMDRTIED-ELENLAVLTVDPSLASHKAHFQYRYTKYLQQKQAIEKYEGSLEDFSKGYLKFGFNREGNKIVYREWAPAAKEAQLIGDFNAWNGLGHQMEKDKFGVWTVYIPDKDGNPAIPHGSNVKIRMKVANGTWIDRIPAWIRYATVDPSNFGAAYDGVYWDPSPSERYEFKYPRPPKPEAPRIYEAHVGMSGIEPRVTSYVEFAENVLPRIKANNYNTVQLMAVAEHSYYGSFGYHVTNFFAPSSRSGTPEDLKFLIDNAHSLGLQVLIDMVHSHASNNVTDGLNGYDIGQRTEESYFHSGERGYHKLWDSRLFNYGNWEVQRFLLSNLRWWLEEFQFDGFRFDGVTSMLYHHHGVNVGFTGNYQEYFGKATDVDAIVYLMLANSLIRNIYPDATVIAEDVSGMPTLGRPVSEGGLGFDYRLAMAIPDKWIEYLKHKRDEQWLMEEIVLTLTNRRYTEKCVAYAESHDQAIVGDKTIAFTLMDKEMYSAMSCLQSLSPVVDRGIALHKMIHFITMALGGEGYLNFMGNEFGHPEWIDFPREGNGWSYEKCRRQWNLVDTDHLRYKFMNNFDKAMNELDEKFHFLASSKQIVSSANEEDKVIVFERGDIVFVFNFHPKNTYSGYKIGCDMPGKYRVALDSDALEFGGHGRVSHDVDHFTSPEGVPGKPETNFNGRPNSFKILMPSRTCLV
P30924_______________SQHSTGLKFGSQERSWDISSTPKSRVRKD-ERMKHSSA----------------ISAVLTDDNSTMAPLEEDVKTENIGLLNLDPTLEPYLDHFRHRMKRYVDQKMLIEKYEGPLEEFAQGYLKFGFNREDGCIVYREWAPAAQEDEVIGDFNGWNGSNHMMEKDQFGVWSIRIPDVDSKPVIPHNSRVKFRFKHGNGVWVDRIPAWIKYATADATKFAAPYDGVYWDPPPSERYHFKYPRPPKPRAPRIYEAHVGMSSSEPRVNSYREFADDVLPRIKANNYNTVQLMAIMEHSYYGSFGYHVTNFFAVSSRYGNPEDLKYLIDKAHSLGLQVLVDVVHSHASNNVTDGLNGFDIGQGSQESYFHAGERGYHKLWDSRLFNYANWEVLRFLLSNLRWWLEEYNFDGFRFDGITSMLYVHHGINMGFTGNYNEYFSEATDVDAVVYLMLANNLIHKIFPDATVIAEDVSGMPGLGRPVSEGGIGFDYRLAMAIPDKWIDYLKNKNDEDWSMKEVTSSLTNRRYTEKCIAYAESHDQSIVGDKTIAFLLMDKEMYSGMSCLTDASPVVDRGIALHKMIHFFTMALGGEGYLNFMGNEFGHPEWIDFPREGNNWSYDKCRRQWNLADSEHLRYKFMNAFDRAMNSLDEKFSFLASGKQIVSSMDDDNKVVVFERGDLVFVFNFHPKNTYEGYKVGCDLPGKYRVALDSDAWEFGGHGRTGHDVDHFTSPEGIPGVPETNFNGRQ------IPSKCCLL

SNPs (tot: 26)

Position	A %	C %	G %	T %	Probability
2044		90		10	0.90298
2045		88		11	0.923564
2066			10	90	0.90298
2096		90		10	0.90298
2128			12	87	0.992554
2129	12			87	0.992554
2136			12	87	0.992554
2137		12	87		0.992554
2138	87	12			0.992554
2139	12			87	0.992554
2141			12	87	0.992554
2142		87	12		0.992554
2145	87	12			0.992554
2147		12		87	0.992554
2165	28	71			1.0
2168	33			66	1.0
2173	33	66			1.0
2174	66		33		1.0
2176		66		33	1.0
2181	60		40		1.0
2184			40	60	1.0
2758	25		75		0.996473
2777	25			75	0.996473
2839		14		85	0.993533
2972		66		33	1.0
3093	33		66		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain