SustainPineDB

Assembly Name: SustainPine v2.0

UniGene Name: sp_v2.0_unigene26818

Length: 2062 nt

Origin of reads: Inia v1 Biogeco1 v2 Biogeco2 v2 Biogeco3 v1 Uagpf v2 Uma v2

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v2.0_unigene26818
ACTTTATCTGCTGCCTAGAGGAGGTTGCCCTTGTCTGGAAGCTGTAGGTTCTGTCATTGAGTGTGATACTCGCGTTGCAATTGAAAGTCAATCTGACCATCAAAGAGAGTATCAAGAAGATGAACAATCTGATCTTCAAGGAAGGGAAGCCAAATATAACGATGAAATACCTGATCATGATGCCAATCATGTTGAAGTATCTGAGCAGAAAGCTGATTCACCATCCTCTACCAAACGCAGAGTAATTGGCCCAGCCATGCCCTCTGCAGAGATGTTGGAAGCTGCTGCTAAACTGACGGAGGCAGAAGCTGCCTTGAGGGATGCTGAGAAGGAATTAGAGAATGATGTGCTGATAGGACCCCCACCACCAGCTGCTGTGGTTGAAGCAGAATCAGCAAATGATGCAGAGCGCTTTGAAGAGGTGACAAGGATTATTCCTCTGAATGCAAATAATCCATATGATGTGTTGGGCTTAAATAGAGGTGCATCAGCTGAGAGCACAAAGAAAAGGTATTGGAAACTGTCTCTTTTAGTACATCCTGATAAATGTTCTCATCCTCAAGCACACCAAGCTTTTACAATATTGAACCAAGCTTTCAAAGATTTACAAGACCCAATAAAACGAGCTGCGATTGACGATAAGATAATAATAAAAGAAGCAAAGGAAGAATTTGAGGCTGACTTGAAGGCACGTCGTGAAGCTGCACAGTGGAGACGTATACGAGGTGAGGCTATATCAGGTGATGATGAGTTGCTAGCTGAAGTTAAACTGTCACCCAAACGAGATGAATGGATGACAACGCTCCCACCTGAAAGAAAGCCTGGCCCAAGTATGCATTCAACATTTTTTAGCAAGTCTGAGAAACCTGAGCGAGGGGATACAAGTACTTGGACTGACACACCATTGGAAAAAGCTCATAAAGCAAAGCAGCATTATTTGCAGGCATACAATCAAGCAGCTCTGACTGCATTAGATGAGGATATTGGGTCCCTTGAAAAGGAACATTCTTCCGCTACAGCAGACCTGATGGATAAATATAACAAGTCTAAGCGTTCTATGTCTCTTGTTGAGAAGCATAAACATGAGCAGAAGAAAAAGTCAAAAAAAGTGGAACAGTCAGAGAAGCAGCAAGAAGAAGAATGGTCAGGAAAGCATCCATGGAAGCCTTGGGACCGAGAGACTGATTTAACAGCTGGGCGGAAGTCTGTGAATTTTGATGCTAATAATTTCACGAAAGGGTTAACTTCAAGATTTTCTTCTGGAAGTGTAGAGAGGAATTTCTTGTAAGTTTAGTCTCTATTTTCATCAAGTATCCGTTCAATTCCTTTGTTCATTATGTGGCTTGTACGTTTTTTCTTCAGCTGTTTCTCTGGATTCTGCCATAGCTGAATTGAACAGACACTCAAGTCACGCAGCAAGTTTATTGATCAGGATGGCTGATTTCTTTGTTTTATATGGAAGAAGAGGAGTATGTGCGGTATTATCTAGAGTAATATTGGGTATCAGATGTCGATCGAAAAATATGGTGGGTTACCAACTGTCAAATAGAGTATAGCGTTCTAGTCACTTTCTAAAACTTAAACGATGAAAGGCGGTGTTCATATTTAAGTGGAACTTGTTTAAATTCACCTTATCTTTTAGCAGATGAGTACATCAGAAACATGAAGTGACAAGGGGTTCTTTTCTCATAGCACATTGAAGTGACAAGTGTTTGAACCTTTGAAAAGGTGAACGAATTAATGTTTCACTTTAAGTCATTTAGATTTGCTTGTATTTTTCAGCAGTTCTATATATGAGAATGGCCGTGCTCGAGGATTATCAGGCATATCGCGAAAGTACTGTTACCAAGATTTAACTTATAACCTTGTAATTTGACGTTGCAAAGATTGTCATCTCGTAATGATAGTCATTTCGATCACACATTTATGACTGATTCAGGTTTCTCTCAGAAATTTCGAATTCCTCTTTTGCCTAATACAAAAATTTTGATTGAAAAAACGTGCATGTCGTTACATGTTTTGTTTAATGGTAAAGCAAAGTGCATACATTTGACGACAGGCC

Ace file of the UniGene sp_v2.0_unigene26818 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	DNAJ heat shock N-terminal domain-containing protein [Arabidopsis thaliana] dbj\|BAE98751.1\| hypothetical protein [Arabidopsis thaliana] gb\|AEE34353.1\| DNAJ heat shock N-terminal domain-containing protein [Arabidopsis thaliana]	-	-	42%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	42%
Blast2go	protein	-	-	66%

Source	InterPros	Term	Type	Identity
Sma3	Leucine-rich repeat	IPR001611	-	-
Sma3	Heat shock protein DnaJ, N-terminal	IPR001623	-	-
Sma3	NB-ARC	IPR002182	-	-
Sma3	Heat shock protein DnaJ	IPR003095	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NV01

Fln msg: No stop codon before M and M found is too far from subject M, your sequence is shorter than subject: 344 - 361

Fln protein: MPSAEMLEAAAKLTEAEAALRDAEKELENDVLIGPPPPAAVVEAESANDAERFEEVTRIIPLNANNPYDVLGLNRGASAESTKKRYWKLSLLVHPDKCSHPQAHQAFTILNQAFKDLQDPIKRAAIDDKIIIKEAKEEFEADLKARREAAQWRRIRGEAISGDDELLAEVKLSPKRDEWMTTLPPERKPGPSMHSTFFSKSEKPERGDTSTWTDTPLEKAHKAKQHYLQAYNQAALTALDEDIGSLEKEHSSATADLMDKYNKSKRSMSLVEKHKHEQKKKSKKVEQSEKQQEEEWSGKHPWKPWDRETDLTAGRKSVNFDANNFTKGLTSRFSSGSVERNFL*

Protein Length: 345

Fln nts: ACTTTATCTGCTGCCTAGAGGAGGTTGCCCTTGTCTGGAAGCTGTAGGTTCTGTCATTGAGTGTGATAaTgGtGTTGCAATTGAAAGTCAATCTGACCATCAAAGAGAGTATCAAGAAGATGAACAATCTGATCTTCAAGGAAGGGAAGCCAAATATAACGATGAAATACCTGATCATGATGCCAATCATGTTGAAGTATCTGAGCAGAAAGCTGATTCACCATCCTCTACCAAACGCAGAGTAATTGGCCCAGCCATGCCCTCTGCAGAGATGTTGGAAGCTGCTGCTAAACTGACGGAGGCAGAAGCTGCCTTGAGGGATGCTGAGAAGGAATTAGAGAATGATGTGCTGATAGGACCCCCACCACCAGCTGCTGTGGTTGAAGCAGAATCAGCAAATGATGCAGAGCGCTTTGAAGAGGTGACAAGGATTATTCCTCTGAATGCAAATAATCCATATGATGTGTTGGGCTTAAATAGAGGTGCATCAGCTGAGAGCACAAAGAAAAGGTATTGGAAACTGTCTCTTTTAGTACATCCTGATAAATGTTCTCATCCTCAAGCACACCAAGCTTTTACAATATTGAACCAAGCTTTCAAAGATTTACAAGACCCAATAAAACGAGCTGCGATTGACGATAAGATAATAATAAAAGAAGCAAAGGAAGAATTTGAGGCTGACTTGAAGGCACGTCGTGAAGCTGCACAGTGGAGACGTATACGAGGTGAGGCTATATCAGGTGATGATGAGTTGCTAGCTGAAGTTAAACTGTCACCCAAACGAGATGAATGGATGACAACGCTCCCACCTGAAAGAAAGCCTGGCCCAAGTATGCATTCAACATTTTTTAGCAAGTCTGAGAAACCTGAGCGAGGGGATACAAGTACTTGGACTGACACACCATTGGAAAAAGCTCATAAAGCAAAGCAGCATTATTTGCAGGCATACAATCAAGCAGCTCTGACTGCATTAGATGAGGATATTGGGTCCCTTGAAAAGGAACATTCTTCCGCTACAGCAGACCTGATGGATAAATATAACAAGTCTAAGCGTTCTATGTCTCTTGTTGAGAAGCATAAACATGAGCAGAAGAAAAAGTCAAAAAAAGTGGAACAGTCAGAGAAGCAGCAAGAAGAAGAATGGTCAGGAAAGCATCCATGGAAGCCTTGGGACCGAGAGACTGATTTAACAGCTGGGCGGAAGTCTGTGAATTTTGATGCTAATAATTTCACGAAAGGGTTAACTTCAAGATTTTCTTCTGGAAGTGTAGAGAGGAATTTCTTGTAA___GTTTAGTCTCTATTTTCATCAAGTATCCGTTCAATTCCTTTGTTCATTATGTGGCTTGTACGTTTTTTCTTCAGCTGTTTCTCTGGATTCTGCCATAGCTGAATTGAACAGACACTCAAGTCACGCAGCAAGTTTATTGATCAGGATGGCTGATTTCTTTGTTTTATATGGAAGAAGAGGAGTATGTGCGGTATTATCTAGAGTAATATTGGGTATCAGATGTCGATCGAAAAATATGGTGGGTTACCAACTGTCAAATAGAGTATAGCGTTCTAGTCACTTTCTAAAACTTAAACGATGAAAGGCGGTGTTCATATTTAAGTGGAACTTGTTTAAATTCACCTTATCTTTTAGCAGATGAGTACATCAGAAACATGAAGTGACAAGGGGTTCTTTTCTCATAGCACATTGAAGTGACAAGTGTTTGAACCTTTGAAAAGGTGAACGAATTAATGTTTCACTTTAAGTCATTTAGATTTGCTTGTATTTTTCAGCAGTTCTATATATGAGAATGGCCGTGCTCGAGGATTATCAGGCATATCGCGAAAGTACTGTTACCAAGATTTAACTTATAACCTTGTAATTTGACGTTGCAAAGATTGTCATCTCGTAATGATAGTCATTTCGATCACACATTTATGACTGATTCAGGTTTCTCTCAGAAATTTCGAATTCCTCTTTTGCCTAATACAAAAATTTTGATTGAAAAAACGTGCATGTCGTTACATGTTTTGTTTAATGGTAAAGCAAAGTGCATACATTTGACGACAGGCC

Fln Alignment:

Contig26818___EVTRIIPLNANNPYDVLGLNRGASAESTKKRYWKLSLLVHPDKCSHPQAHQAFTILNQAFKDL
A9NV01_______________EIVRLIKRNKDY-YVILGLEKNCSVEDVRKAYRKLSLRVHPDKNKAPGSEEAFKAVSKAFQCL

SNPs (tot: 6)

Position	A %	C %	G %	T %	Probability
1371	23		76		1.0
1598	83	16			0.994512
1685	80	20			0.995493
1688			80	20	0.995493
1857		16		83	0.994512
1876			83	16	0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain