SustainPineDB

Assembly Name: uagpf v2.0

UniGene Name: uagpf_v2_unigene20251

Length: 1677 nt

UniGene Fasta

Ace file of the UniGene uagpf_v2_unigene20251

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene23016	26/47
SustainPine v2.0	sp_v2.0_unigene23289	1/47
SustainPine v2.0	sp_v2.0_unigene29286	6/47
SustainPine v2.0	sp_v2.0_unigene76934	10/47
SustainPine v2.0	sp_v2.0_unigene79139	3/47

Source	Descriptions	Term	Type	Identity
AutoFact	Phosphoglycerate kinase n=4 Tax=Picea sitchensis RepID=A9NW50_PICSI	-	-	78%
FL-Next	sp=Phosphoglycerate kinase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	74%
Blast2go	phosphoglycerate kinase	-	-	75%

Source	InterPros	Term	Type	Identity
Sma3	Aminoacyl-tRNA synthetase, class I, conserved site	IPR001412	-	-
Sma3	Phosphoglycerate kinase	IPR001576	-	-
Sma3	Phosphoglycerate kinase, N-terminal	IPR015824	-	-
Sma3	Phosphoglycerate kinase, C-terminal	IPR015901	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LLY9

Fln msg: Overlapping hits, possible frame ERROR between 784 and 756,

Fln protein: MTKYNSENGLGVGYSVEDGTDQVEIDPRLPLLQDADLRDKVVLLRVDHNVVKKGKILDQYRIDVTLPTMYNIVERGGRPIIMTHVGRPYDKKTKTISTLQDESVDAVVAYLQRKLHIKFVVPRLNTTEKNGILDIDTSINWLIHDLKSRKIGGIYLPNTRWFAGEEDKGEKLNRFVLQLSALADVYVNDASxxxxxxxxxxxHIAKYLPSYAGLCMQGELYHVQSVLNPSIPFVAIVAGAKYDTKIGPLKQMYKKVDKLVLGGLIYNTYLCAKYGVKIAGVEESDIELAKELVEQDKVHKKILELQVLVETDTLDGRFEGKFRTIDVKDFAPGQTYKYVVDVDPVSFERDEAKQAMNSAKTIFVNAVMGLMPHFWHGTSKMDEVIDKNRSAQKFFGGGDTLQTFKSLHPALYEAALNNIKYYLFTGGGTVLKVIEEGDPMGLETVKALIDNKANYIKRSQNN*

Protein Length: 464

Fln nts: CGTCGTGACCTTGGCTGTCACTCAGGTTTACTTGAAATCCCACGGAACCTAGACAAATACCGACGAATTTGGGGGTGTCGCTGGTAAAGATGGCTCGATCGAGCATATATAAATTAAGCGAGAGGGTCGATGCTCATAACGAACGAGGGATCTGTAATCAAGGAAATACTAATCAAGAACT_-_ATGACTAAGTATAATTCAGAGAATGGTCTGGGTGTGGGTTACAGCGTGGAGGATGGAACTGACCAAGTGGAGATCGATCCGAGGTTGCCTCTGCTGCAGGATGCTGATCTCAGAGACAAGGTCGTTCTTCTCAGGGTCGATCACAACGTTGTGAAGAAAGGTAAAATACTAGATCAATACAGAATCGATGTCACTTTACCAACTATGTACAACATAGTCGAGCGAGGTGGAAGGCCCATTATCATGACTCACGTTGGACGCCCATATGATAAGAAGACAAAAACTATCTCTACTCTTCAGGATGAATCTGTGGATGCTGTTGTTGCTTATTTGCAAAGGAAGCTCCACATCAAATTTGTAGTTCCCCGCTTAAATACAACGGAGAAAAATGGAATTCTGGATATTGACACTTCCATCAACTGGCTGATCCATGACCTTAAGAGTCGGAAGATTGGTGGAATCTACCTGCCAAATACTCGGTGGTTTGCAGGGGAGGAAGACAAAGGAGAGAAGCTTAACAGATTTGTCCTTCAGCTCTCTGCTTTAGCTGATGTATATGTTAATGATGCATCTxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxCATATTGCAAAATACCTTCCCTCATATGCAGGACTATGCATGCAGGGCGAGCTTTATCATGTGCAATCTGTCTTGAATCCCAGCATACCATTTGTTGCAATTGTTGCTGGTGCAAAGTATGATACTAAGATTGGTCCATTAAAACAGATGTACAAGAAAGTTGATAAGTTGGTTCTGGGTGGTCTCATTTACAATACTTATCTATGTGCCAAATATGGAGTTAAAATTGCTGGTGTTGAGGAATCAGATATTGAACTAGCAAAAGAGCTGGTAGAACAGGATAAGGTGCACAAAAAAATTCTCGAGTTGCAGGTTCTGGTTGAAACTGATACTCTGGATGGTAGATTTGAAGGGAAATTCAGAACGATTGATGTCAAGGACTTTGCTCCAGGCCAGACTTACAAGTATGTTGTTGATGTTGACCCTGTCTCATTTGAGAGAGATGAGGCTAAACAAGCAATGAACTCAGCTAAGACAATATTTGTTAATGCTGTGATGGGACTCATGCCACATTTCTGGCACGGTACAAGCAAAATGGATGAGGTCATTGATAAAAATCGTTCTGCTCAAAAATTCTTTGGAGGTGGTGATACCCTTCAGACTTTTAAAAGCCTTCATCCAGCCCTGTATGAAGCTGCATTGAACAATATCAAATACTATTTATTTACAGGCGGTGGCACTGTTTTGAAAGTTATTGAAGAGGGAGATCCAATGGGTCTAGAGACTGTGAAGGCTCTTATTGACAACAAAGCTAACTATATCAAGAGAAGTCAAAATAATTGA___AGCTAGTTTATGGCAAGAGGTTTTTGGCTTGCAGAATAAGGGATTGATTAGCTCGTGCTGTTTGTTAGAACGAAAAGCAGCACGTAGAGATTTACGAAAGGATATTTGG

Fln Alignment:

Contig20251___MTKYNSENGLG-VGYSVEDGTDQVEI-DPRLPLLQDADLRDKVVLLRVDHNVVKKGKILDQYRIDVTLPTMYNIVERGGRPIIMTHVGRPYDKKTKTISTLQDESVDAVVAYLQRKLHIKFVVPRLNTTEKNGILDIDTSINWLIHDLKSRKIGGIYLPNTRWFAGEEDKGEKLNRFVLQLSALADVYVNDA-xxxxxxxxxxxxAKYLPSYAGLCMQGELYHVQSVLNPSIPFVAIVAGAKYDTKIGPLKQMYKKVDKLVLGGLIYNTYLCAKYGVKIAGVEESDIELAKELVEQDKVHKKILELQVLVETDTLDGRFEGKFRTIDVKDFAPGQTYKYVVDVDPVSFERDEAKQAMNSAKTIFVNAVMGLMPHFWHGTSKMDEVIDKNRSAQKFFGGGDTLQTFKSLHPALYEAALNNIKYYLFTGGGTVLKVIEEGDPMGLETVKALIDNK
B8LLY9________________MAPYGQENGLTRIGYSVDDGLENDKIADPRLPLLPDADLKDKIVLLRVDHNVVKKGEILDQYRIDVTLPTLYNITERGGRPILMTHVGRPYDKKTKTITIRKDESVDAIVAYLQRKLRIKFVVPKFKYDEKDGILDIDTSIKWLITDLRKRKIGGIYLPNTRWFAGEEDKGEKLERFAIQLAGLADVFVNDAFxxxxxxxxxxxxTKYLPSYAGLCMQDELYHVEAVLNPTRPFIAIVAGSKYDTKIGPLNQIYKKVDKLVLGGVIYNTYLCAKYGVKIAGVQDSDIELAKELVEQDKTQGKIVEMDILVETDTLSGRFEGKYRAVNVKDFAPGKTYNYIVDIDPLSLESEDVLQALNSAKTIFVNAVMGFMPNFWHGTSKLDEVIDNNQSAKKYFGGGDTLQAFKSLHPNLYQAALDNTNYYLFTGGGTVLKVIEEGDSMGLDTVRALIENK

SNPs (tot: 156)

Position	A %	C %	G %	T %	Probability
41		66	33		0.997452
42	66			33	0.997452
45			66	33	0.997452
46	66		33		0.997452
51	66			33	0.997452
53	66		33		0.997452
55	66			33	0.997452
56	66		33		0.997452
57	66			33	0.997452
58		33		66	0.997452
61		66		33	0.997452
62		33	66		0.997452
141	12		87		0.992554
143	87			12	0.992554
427		18	81		0.999994
439	88	11			0.923564
440		88		11	0.923564
441	11		88		0.923564
447	88			11	0.923564
448		11		88	0.923564
450	88	11			0.923564
508			11	88	0.923564
509	11			88	0.923564
510	88	11			0.923564
511			11	77	0.968132
513			11	88	0.923564
514	11		88		0.923564
516	88	11			0.923564
517	88			11	0.923564
518	77	11		11	0.968132
521	77	11			0.968132
522	88			11	0.923564
540		28		71	1.0
548		71		28	1.0
553	12	87			0.992554
555	75		25		1.0
562	28		71		1.0
592		28		71	1.0
622	14		85		0.993533
647	28	71			1.0
710	40	60			1.0
711	40	60			1.0
725		50		50	1.0
726		33		66	1.0
728	83		16		0.994512
733	83	16			0.994512
734	16			83	0.994512
735		16	83		0.994512
737	83	16			0.994512
738	16			83	0.994512
752	66		33		1.0
755			33	66	1.0
758	33		66		1.0
762	62			37	1.0
767	50			50	1.0
786	50			50	1.0
798	42		57		1.0
803	66		33		1.0
829	88		11		0.923564
831		11	88		0.923564
832		88	11		0.923564
833	88			11	0.923564
835	12		87		0.992554
836		12	87		0.992554
837		75		25	1.0
838			85	14	0.993533
839	87		12		0.992554
840	50		50		1.0
844		12		87	0.992554
875	14			85	0.993533
903	85	14			0.993533
927	66	33			1.0
939	20		80		0.995493
959		20		80	0.995493
972	20			80	0.995493
999		60		40	1.0
1049	75	25			0.996473
1052		75	25		0.996473
1054	75	25			0.996473
1055	75	25			0.996473
1056	75			25	0.996473
1057			75	25	0.996473
1058	75		25		0.996473
1063			75	25	0.996473
1064		25		75	0.996473
1066		25	75		0.996473
1067	75			25	0.996473
1068	75	25			0.996473
1069	25	75			0.996473
1095		66		33	0.997452
1110		25	75		0.996473
1113		25		75	0.996473
1115	75			25	0.996473
1116	75		25		0.996473
1119		25		75	0.996473
1120			75	25	0.996473
1121	75			25	0.996473
1122			25	75	0.996473
1123	75		25		0.996473
1126		75	25		0.996473
1129		25	75		0.996473
1131		25		75	0.996473
1132			75	25	0.996473
1133		25	75		0.996473
1134	25			75	0.996473
1140		20		80	0.995493
1142	80			20	0.995493
1143	80	20			0.995493
1145			80	20	0.995493
1148	80	20			0.995493
1149	80	20			0.995493
1152		60	20	20	0.99255
1153	80		20		0.995493
1154		20	80		0.995493
1155	80			20	0.995493
1156	80		20		0.995493
1157		80		20	0.995493
1158		20	80		0.995493
1160		20		80	0.995493
1162		20	80		0.995493
1186		80		20	0.995493
1187	80			20	0.995493
1188		20	80		0.995493
1189	80		20		0.995493
1248	66	33			1.0
1250		66		33	1.0
1251	66	33			1.0
1252	66		33		1.0
1256	66	33			1.0
1259		66		33	1.0
1260	66		33		1.0
1264	66		33		1.0
1265	66			33	1.0
1266		33	66		1.0
1271		33		66	1.0
1276	16		83		0.994512
1289		16		83	0.994512
1297	83			16	0.994512
1298		16		83	0.994512
1369			12	87	0.992554
1371		12		87	0.992554
1375		12	87		0.992554
1376		12	87		0.992554
1378			87	12	0.992554
1382	87	12			0.992554
1384	87		12		0.992554
1385		87		12	0.992554
1387	12	87			0.992554
1388		12		87	0.992554
1417	14	85			0.993533
1510		66		33	1.0
1521	16		83		0.994512
1523	83		16		0.994512
1552	80			20	0.995493
1560	80	20			0.995493
1562			80	20	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain