SustainPineDB

Assembly Name: uagpf v2.0

UniGene Name: uagpf_v2_unigene18790

Length: 2165 nt

UniGene Fasta

>uagpf_v2_unigene18790
CGTCGTGACCTTGGCTGTCACTCAAATCTTACCTACTTTCAGGACCAAACATCAGTCACGGCAAAAGATCGAACTATCTTCTCCTCCACCACTATAAATATTACACGATCCCAGCCTCTATCCTGTGCCACACAGCCAAGCTAAAGCAAGGTCTGTGGATTTCCGGAATCTCTAGATCTGGGCTAACGTCGTGACCTTGGCTGTCACTCATTTTTTCATCTGGGCATTACTAGTGTGCCCTTTCCTACTTTGGGTTTGCTTTTGCATTTCCTTGTTTCGTTCTGAACAATGGATCCCGTGAACGTATGGGGCAACACGCCACTGAAGGTTGTGGACGAGGAGATCTTTGATCTGATTGAGAAGGAGAAGAGAAGGCAATGCAGGGGAATCGAACTCATAGCCTCCGAGAACTTCACATCCCTGGCAGTCATTGAAGCTCTTGGAACCCCCCTCACAAACAAATACTCCGAGGGCATGCCTGGCAACAGATACTACGGTGGTAACGAATACATAGATCTCATTGAAAATCTGTGCAGATCAAGGGCCCTCCAGGCCTTCCATCTGGATTCCGAGAAATGGGGTGTGAATGTGCAGCCCTACTCTGGGAGCCCCGCTAACTTCGCAGCCTACACAGCCCTGCTGAACCCACATGACCGCATCATGGGGCTGGATTTGCCTTTGGTTGTCACTCACTCATGGATATTACACCTCTGGTGGCAAGAAGATTTCTGCTACATCTATTTACTTTGAGAGTTTGCCTTACAAGGTCAGCCAGGAGACTGGGTTCATTGATTATGACAAGCTGGAGGAGAAAGCCTTGGATTTCAGGCCTAAGCTTATTATATGTGGAGGAAGTGCTTATCCCAGGGACTGGGATTATGCTAGGTTCCGTTCCATTGCTGACAAATGTGGTGCCATGCTCTTATGTGATATGGCTCACATCAGTGGCCTTGTTGCTGCTCAGGAAGCTGGAAATCCATTTGACTATTGTGATTTGGTTACCACAACCACCCACAAGAGTTTGAGGGGTCCAAGAGCAGGGATGATCTTCTATAGGAAAGGCCCAAAGCCTCCTAAAAAGGGACAGCCAGAAGGTGCCCTCTATGATTACGAGGACAGAGTAAACTTTTCTGTGTTTCCGTCCCTTCAAGGAGGGCCACACAACCACCAGATTGCAGCATTGGCTGTGGCTCTTAAGCAGGTTATGACTCCTGGTTTTAAGGCCTATGCCAAGCAGGTCAAGGCAAATGCGGTTGCTGTTGGTAACTACTTGATGAACAAGGGATACAAGCTTGTTACAAGTGGGACTAGAAAATCATCTAGTTCTGTGGGATCTCCGGCCAATTGGACTGACTGGAAATAAGGTGGAGAAAGTGTGTGACCTGTGTAATATAACAATCAACAAGAATGCAGTTTATGGTGACAGTAGTGCTCTGTCCCCTGGGGGTGTTCGTATTGGTACCCCTGCTATGACCTCCAGAGGTCTGAAGGAAGCTGATTTTGAGCAGATTGGAGAGTTCCTCCACCAGTCTATTATCATCACTTTGGCTATCCAGAGCGAACATGGGAAGCTTCTCAAGGACTTCAACAAAGGTTTGGCGGGCAACAAGGACATTGAAAATCTGAAAGCTCAAGTGGAGAAATTCTCTGCACAGTTTGATATGCCTGGCTTTGATGTAGCTACAATGAAGTTCCAATAAGTGATCATAGGAATAGCATAATTCCTTTTCCTTTTGGGTGCATTGCTGTTAGCATTGAAAGTTGGTAGCTAGAGGGATATATTCTGTAGCTTGATGAATAAATATGTATTGTTCGTGAGTTTCAGAGTCCTCAAAATTTGAGGACTTATTTACTGGTGTAATCAGTAGAAGATGACTAAATTTATTAGTCAGATGTGTTGAATTGGTTTTTCAGTTAAACATACACAAAGTCTAGTCTTTTTCCGGTGTCATCTTTGTAATCTTCTGCTTTTGTTTACATGTCCCATGCATTGGGCAAATTCTGCAATTGGAGCTATGCAATCTTATTTTGTTAGCCGATTAAAAGAAATAATCGGTTTAAATAAACAAGTTACTTTTGTGACTAGAGGGGAACTATACATAGCCCCATATATTTTTTTAAAATAAAAAATGTTTTGCTCTTACTCCATGGGAGTTTGTGAGGGA

Ace file of the UniGene uagpf_v2_unigene18790

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene18302	10/155
SustainPine v2.0	sp_v2.0_unigene25693	112/155
SustainPine v2.0	sp_v2.0_unigene61494	21/155
SustainPine v2.0	sp_v2.0_unigene74652	1/155

Source	Descriptions	Term	Type	Identity
AutoFact	Serine hydroxymethyltransferase n=2 Tax=Picea sitchensis RepID=B8LLP6_PICSI	-	-	95%
FL-Next	sp=Serine hydroxymethyltransferase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	97%
Blast2go	serine hydroxymethyltransferase	-	-	92%

Source	GOs	Term	Type	Identity
Blast2go	response to cadmium ion	GO:0046686	Biological Process	92%
Blast2go	small molecule metabolic process	GO:0044281	Biological Process	92%
Blast2go	cellular metabolic process	GO:0044237	Biological Process	92%
Blast2go	transferase activity	GO:0016740	Molecular Function	92%
Blast2go	plasma membrane	GO:0005886	Cellular Component	92%

Source	InterPros	Term	Type	e value	Identity
Sma3	Serine hydroxymethyltransferase	IPR001085	-	0.0	-
Sma3	Pyridoxal phosphate-dependent transferase, major region, subdomain 1	IPR015421	-	0.0	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LLP6

Fln msg: Separated hits, possible frame ERROR between 678 and 689, and possible frame ERROR between 1294 and 1305,

Fln protein: MDPVNVWGNTPLKVVDEEIFDLIEKEKRRQCRGIELIASENFTSLAVIEALGTPLTNKYSEGMPGNRYYGGNEYIDLIENLCRSRALQAFHLDSEKWGVNVQPYSGSPANFAAYTALLNPHDRIMGLDLxxxxxLTHGYYTSGGKKISATSIYFESLPYKVSQETGFIDYDKLEEKALDFRPKLIICGGSAYPRDWDYARFRSIADKCGAMLLCDMAHISGLVAAQEAGNPFDYCDLVTTTTHKSLRGPRAGMIFYRKGPKPPKKGQPEGALYDYEDRVNFSVFPSLQGGPHNHQIAALAVALKQVMTPGFKAYAKQVKANAVAVGNYLMNKGYKxxxxVGLENHLVLWDLRPIGLTGNKVEKVCDLCNITINKNAVYGDSSALSPGGVRIGTPAMTSRGLKEADFEQIGEFLHQSIIITLAIQSEHGKLLKDFNKGLAGNKDIENLKAQVEKFSAQFDMPGFDVATMKFQ*

Protein Length: 473

Fln nts: CGTCGTGACCTTGGCTGTCACTCAAATCTTACCTACTTTCAGGACCAAACATCAGTCACGGCAAAAGATCGAACTATCTTCTCCTCCACCACTATAAATATTACACGATCCCAGCCTCTATCCTGTGCCACACAGCCAAGCTAAAGCAAGGTCTGTGGATTTCCGGAATCTCTAGATCTGGGCTAACGTCGTGACCTTGGCTGTCACTCATTTTTTCATCTGGGCATTACTAGTGTGCCCTTTCCTACTTTGGGTTTGCTTTTGCATTTCCTTGTTTCGTTCTGAACA_-_ATGGATCCCGTGAACGTATGGGGCAACACGCCACTGAAGGTTGTGGACGAGGAGATCTTTGATCTGATTGAGAAGGAGAAGAGAAGGCAATGCAGGGGAATCGAACTCATAGCCTCCGAGAACTTCACATCCCTGGCAGTCATTGAAGCTCTTGGAACCCCCCTCACAAACAAATACTCCGAGGGCATGCCTGGCAACAGATACTACGGTGGTAACGAATACATAGATCTCATTGAAAATCTGTGCAGATCAAGGGCCCTCCAGGCCTTCCATCTGGATTCCGAGAAATGGGGTGTGAATGTGCAGCCCTACTCTGGGAGCCCCGCTAACTTCGCAGCCTACACAGCCCTGCTGAACCCACATGACCGCATCATGGGGCTGGATTTGCCxxxxxxxxxxxxACTCACTCATGGATATTACACCTCTGGTGGCAAGAAGATTTCTGCTACATCTATTTACTTTGAGAGTTTGCCTTACAAGGTCAGCCAGGAGACTGGGTTCATTGATTATGACAAGCTGGAGGAGAAAGCCTTGGATTTCAGGCCTAAGCTTATTATATGTGGAGGAAGTGCTTATCCCAGGGACTGGGATTATGCTAGGTTCCGTTCCATTGCTGACAAATGTGGTGCCATGCTCTTATGTGATATGGCTCACATCAGTGGCCTTGTTGCTGCTCAGGAAGCTGGAAATCCATTTGACTATTGTGATTTGGTTACCACAACCACCCACAAGAGTTTGAGGGGTCCAAGAGCAGGGATGATCTTCTATAGGAAAGGCCCAAAGCCTCCTAAAAAGGGACAGCCAGAAGGTGCCCTCTATGATTACGAGGACAGAGTAAACTTTTCTGTGTTTCCGTCCCTTCAAGGAGGGCCACACAACCACCAGATTGCAGCATTGGCTGTGGCTCTTAAGCAGGTTATGACTCCTGGTTTTAAGGCCTATGCCAAGCAGGTCAAGGCAAATGCGGTTGCTGTTGGTAACTACTTGATGAACAAGGGATACAAGxxxxxxxxxxxxGTGGGACTAGAAAATCATCTAGTTCTGTGGGATCTCCGGCCAATTGGACTGACTGGAAATAAGGTGGAGAAAGTGTGTGACCTGTGTAATATAACAATCAACAAGAATGCAGTTTATGGTGACAGTAGTGCTCTGTCCCCTGGGGGTGTTCGTATTGGTACCCCTGCTATGACCTCCAGAGGTCTGAAGGAAGCTGATTTTGAGCAGATTGGAGAGTTCCTCCACCAGTCTATTATCATCACTTTGGCTATCCAGAGCGAACATGGGAAGCTTCTCAAGGACTTCAACAAAGGTTTGGCGGGCAACAAGGACATTGAAAATCTGAAAGCTCAAGTGGAGAAATTCTCTGCACAGTTTGATATGCCTGGCTTTGATGTAGCTACAATGAAGTTCCAATAA___GTGATCATAGGAATAGCATAATTCCTTTTCCTTTTGGGTGCATTGCTGTTAGCATTGAAAGTTGGTAGCTAGAGGGATATATTCTGTAGCTTGATGAATAAATATGTATTGTTCGTGAGTTTCAGAGTCCTCAAAATTTGAGGACTTATTTACTGGTGTAATCAGTAGAAGATGACTAAATTTATTAGTCAGATGTGTTGAATTGGTTTTTCAGTTAAACATACACAAAGTCTAGTCTTTTTCCGGTGTCATCTTTGTAATCTTCTGCTTTTGTTTACATGTCCCATGCATTGGGCAAATTCTGCAATTGGAGCTATGCAATCTTATTTTGTTAGCCGATTAAAAGAAATAATCGGTTTAAATAAACAAGTTACTTTTGTGACTAGAGGGGAACTATACATAGCCCCATATATTTTTTTAAAATAAAAAATGTTTTGCTCTTACTCCATGGGAGTTTGTGAGGGA

Fln Alignment:

Contig18790___MDPVNVWGNTPLKVVDEEIFDLIEKEKRRQCRGIELIASENFTSLAVIEALGTPLTNKYSEGMPGNRYYGGNEYIDLIENLCRSRALQAFHLDSEKWGVNVQPYSGSPANFAAYTALLNPHDRIMGLDLPxxxxLTHGYYTSGGKKISATSIYFESLPYKVSQETGFIDYDKLEEKALDFRPKLIICGGSAYPRDWDYARFRSIADKCGAMLLCDMAHISGLVAAQEAGNPFDYCDLVTTTTHKSLRGPRAGMIFYRXXXXXXXXXXXEGALYDYEDRVNFSVFPSLQGGPHNHQIAALAVALKQVMTPGFKAYAKQVKANAVAVGNYLMNKGYKLxxxxGLENHLVLWDLRPIGLTGNKVEKVCDLCNITINKNAVYGDSSALSPGGVRIGTPAMTSRGLKEADFEQIGEFLHQSIIITLAIQSEHGKLLKDFNKGLAGNKDIENLKAQVEKFSAQFDMPGFDVATMKFQ
B8LLP6________________MDPVNEWGNTPLKVVDEEIFDLIEKEKRRQCRGIELIASENFTSLAVIEALGTPLTNKYSEGMPGNRYYGGNEFIDLIENLCRSRALEAFHLDSEKWGVNVQPYSGSPANFAAYTALLNPHDRIMGLDLPxxxxLTHGYYTSGGKKISATSIYFESLPYKVSQETGFIDYDKLEEKALDFRPKLIICGGSAYPRDWDYARFRSIADKCGAMLLCDMAHISGLVAAQEAGNPFDYCDLVTTTTHKSLRGPRAGMIFYRKGPKPPKKGQPEGALYDYEDRVNFSVFPSLQGGPHNHQIAALAVALKQVMTPGFKAYAKQVKANAVAVGNYLMNKGYKLxxxxGTENHLVLWDLRPIGLTGNKVEKVCDLCNITINKNAVYGDSSALSPGGVRIGTPAMTSRGLKEADFEQIGEFLHQSINITLSIQKEYGKLLKDFNKGLAGNKDMENLKAEVEKFSAKFDMPGFDVATMKYQ

SNPs (tot: 177)

Position	A %	C %	G %	T %	Probability
188	40		60		1.0
191		40	60		1.0
192			40	60	1.0
195		60		40	1.0
197	40			60	1.0
199			60	40	1.0
200			60	40	1.0
203		40	60		1.0
204		40		60	1.0
205		60		40	1.0
206	60			40	1.0
208		25		75	0.996473
220	80		20		0.995493
225		16	83		0.994512
226	16		83		0.994512
227		16	66	16	0.988813
229	66		16	16	0.988813
231			33	66	1.0
232	83		16		0.994512
235	66			16	0.988813
238	16		83		0.994512
239		16		83	0.994512
240	16		66	16	0.988813
242	16	83			0.994512
243	16	83			0.994512
265	16			83	0.994512
310	85	14			0.993533
311			14	85	0.993533
312			85	14	0.993533
314	14		85		0.993533
315		14	85		0.993533
317	85			14	0.993533
318	85			14	0.993533
319		85	14		0.993533
320	85		14		0.993533
323		85		14	0.993533
328		14	85		0.993533
401	87		12		0.992554
403	87		12		0.992554
405	12	87			0.992554
408		87		12	0.992554
409		87	12		0.992554
411	87			12	0.992554
413	87	12			0.992554
470			25	75	0.996473
471		75		25	0.996473
474	75			25	0.996473
476	25		75		0.996473
477		25	75		0.996473
479	75			25	0.996473
482		75	25		0.996473
486			75	25	0.996473
491	75			25	0.996473
492		25	75		0.996473
564	75		25		0.996473
569		25	75		0.996473
570	75	25			0.996473
573		75	25		0.996473
574		75	25		0.996473
575		25	75		0.996473
576	75			25	0.996473
578	75			25	0.996473
579	75	25			0.996473
582		25	75		0.996473
583			75	25	0.996473
584		25	75		0.996473
585	25		75		0.996473
673		40	60		1.0
675	60			40	1.0
676		40		60	1.0
677			40	60	1.0
680	40	60			1.0
682		50		50	1.0
688		50		50	1.0
689			50	50	1.0
691		50		50	1.0
823		75	25		0.996473
825		25		75	0.996473
829		25		75	0.996473
830		25		75	0.996473
833	75			25	0.996473
837		75	25		0.996473
839	75	25			0.996473
844		25		75	0.996473
1006		12		87	0.992554
1007	87		12		0.992554
1008		87		12	0.992554
1010	87		12		0.992554
1011		87		12	0.992554
1012	87		12		0.992554
1017		87		12	0.992554
1018		87		12	0.992554
1019		87	12		0.992554
1020	87		12		0.992554
1022	87			12	0.992554
1023	87		12		0.992554
1024			87	12	0.992554
1025	87	12			0.992554
1026	12		87		0.992554
1027		12		87	0.992554
1029		12		87	0.992554
1030	12		87		0.992554
1201			42	57	1.0
1203	14		42		1.0
1205	57	42			1.0
1206		42	57		1.0
1207		50		50	1.0
1208	50			50	1.0
1211		50		50	1.0
1213	50		50		1.0
1215		50	50		1.0
1221	33			50	1.0
1222	16		83		0.994512
1257		14		85	0.993533
1262	16			83	0.994512
1263		16		83	0.994512
1264		16	83		0.994512
1265		83		16	0.994512
1268			16	83	0.994512
1269		16		83	0.994512
1270			83	16	0.994512
1273	83	16			0.994512
1277	83	16			0.994512
1278	16	83			0.994512
1288	85			14	0.993533
1289	85	14			0.993533
1291			85	14	0.993533
1295	85	14			0.993533
1297	85			14	0.993533
1298	85			14	0.993533
1300		85	14		0.993533
1301		14		85	0.993533
1305		14		85	0.993533
1308	83			16	0.994512
1309	62	37			1.0
1310	12		87		0.992554
1314			75	25	1.0
1318		25	12		1.0
1320	66	11		22	0.999999
1321		10	90		0.90298
1322	60		40		1.0
1323	70	20		10	0.999998
1324	60	20		20	0.999999
1325	60	10	30		1.0
1328	90		10		0.90298
1329		10	10	60	0.999999
1332	60	10	10		0.999999
1335	10			90	0.90298
1337	10			90	0.90298
1339		10		90	0.90298
1341		10	90		0.90298
1342	10		90		0.90298
1435	90	10			0.90298
1438	90	10			0.90298
1442	10	90			0.90298
1443		10		90	0.90298
1449		88	11		0.923564
1450		88	11		0.923564
1454			88	11	0.923564
1455		11	88		0.923564
1456	11		88		0.923564
1457		11	88		0.923564
1459	11		88		0.923564
1566			88	11	0.999952
1652	84	15			0.999986
1665		14	85		0.999981
1753		6		93	0.999264
1779		93		6	0.99903
1782		6	93		0.99903
1796	48		51		1.0
1948	4			71	1.0
1957		8		91	0.999807
1960		78	4	4	1.0
1961	78	4		13	1.0
1962		4	8	86	0.999539
1964			14	85	1.0
1965		14		85	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain