SustainPineDB

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Assembly info
Unigenes
SNPs
SSRs
Descriptions
GOs
ECs
KEGGs
InterPros
miRNAs

Assembly Name: uagpf v2.0

UniGene Name: uagpf_v2_unigene2464

Length: 536 nt

UniGene Fasta

>uagpf_v2_unigene2464
CGTCGTGACCTTGGCTGTCACTCAGTCGATAATTTTTGAAAAGTCGGGAGCCGACTGTGCCAGAGATTTCGGCCGGAAAAGCAGTCCCTCTAGAAAGTCAGCTTGACAGCACCTGTAGGGTTTGTTCAAAGCTCGTGGATTGAAAAGAAGACCCGAATCAGCGAAGTCGTGTCCTTAAAGTAGCAATTAACAGCTTGATCTCTGATCCTTTGCATCCAGATCAAGTTTGCAATTCAAAGGATTCATATACAAAGCCGAATGATTGCCATTTGATGCAACTTAGTGGAGTTGGAGCCATTTAGCAGTGACGAATCTCGAAGAAAAGGAAAGCTCTGAGCCATGGCAAATGCAGAGAGTTACATTGTAAAGGTCGAGGATGGCAAACCGTCTCGAGATGGCAGGCCTTCGATGGGGCATGTCTATCGCAGCATTGATGCGAAGAACGGATTCCCGAGCCCTCCGCAGGGGATTGAGAGCTGTTGGGACATCTTCAGGTAAGTCTTTATTGTTCTCAGAGGTTCAGTTTCAGCATTCGA

Ace file of the UniGene uagpf_v2_unigene2464

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Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene5898	8/9

Source	Descriptions	Term	Type	Identity
AutoFact	Acyl CoA synthetase, putative n=1 Tax=Ricinus communis RepID=B9RZF1_RICCO	-	-	58%
FL-Next	sp=Long chain acyl-CoA synthetase 4; Arabidopsis thaliana (Mouse-ear cress).	-	-	59%
Blast2go	annotation was added to scaffolds in november 2011~long-chain-fatty-acid- ligase	-	-	83%

Source	ECs	Term	Type	e value	Identity
Blast2go	Long-chain-fatty-acid--CoA ligase.	EC:6.2.1.3	-	0.0	83%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Fatty acid metabolism	00071		0.0	83%
Blast2go	Metabolic pathways	01100		0.0	83%

Source	GOs	Term	Type	Identity
Blast2go	fatty acid biosynthetic process	GO:0006633	Biological Process	83%
Blast2go	long-chain fatty acid-CoA ligase activity	GO:0004467	Molecular Function	83%
Blast2go	plasma membrane	GO:0005886	Cellular Component	83%

Source	InterPros	Term	Type	e value	Identity
Sma3	AMP-dependent synthetase/ligase	IPR000873	-	0.0	-

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: sp_plants

Fln subject: Q9T0A0

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 600 aas, your sequence is shorter than subject: 53 - 666

Fln protein: MANAESYIVKVEDGKPSRDGRPSMGHVYRSIDAKNGFPSPPQGIESCWDIFR*

Protein Length: 54

Fln nts: CGTCGTGACCTTGGCTGTCACTCAGTCGATAATTTTTGAAAAGTCGGGAGCCGACTGTGCCAGAGATTTCGGCCGGAAAAGCAGTCCCTCTAGAAAGTCAGCTTGACAGCACCTGTAGGGTTTGTTCAAAGCTCGTGGATTGAAAAGAAGACCCGAATCAGCGAAGTCGTGTCCTTAAAGTAGCAATTAACAGCTTGATCTCTGATCCTTTGCATCCAGATCAAGTTTGCAATTCAAAGGATTCATATACAAAGCCGAATGATTGCCATTTGATGCAACTTAGTGGAGTTGGAGCCATTTAGCAGTGACGAATCTCGAAGAAAAGGAAAGCTCTGAGCC_-_ATGGCAAATGCAGAGAGTTACATTGTAAAGGTCGAGGATGGCAAACCGTCTCGAGATGGCAGGCCTTCGATGGGGCATGTCTATCGCAGCATTGATGCGAAGAACGGATTCCCGAGCCCTCCGCAGGGGATTGAGAGCTGTTGGGACATCTTCAGGTAAGTCTTTATTGTTCTCAGAGGTTCAGTTTCAGCATTCGA

Fln Alignment:

Contig2464___MANAESYIVKVEDGKPSRDGRPSMGHVYRSIDAKNGFPSPPQGIESCWDIFR
Q9T0A0_______________MSQQKKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFR

SNPs (tot: 5)

Position	A %	C %	G %	T %	Probability
308	55	44			1.0
353	57		42		1.0
386	33	66			1.0
387	33		66		1.0
450		66		33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain