SustainPineDB

Assembly Name: biogeco2 v2.0

UniGene Name: biog2_v2.0_unigene5312

Length: 1424 nt

UniGene Fasta

Ace file of the UniGene biog2_v2.0_unigene5312

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene16051	1/76
SustainPine v2.0	sp_v2.0_unigene16257	75/76

Source	Descriptions	Term	Type	Identity
AutoFact	Malate dehydrogenase n=10 Tax=Pinaceae RepID=A9NVT9_PICSI	-	-	92%
FL-Next	sp=Malate dehydrogenase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	93%
Blast2go	malate dehydrogenase	-	-	95%

Source	GOs	Term	Type	Identity
Blast2go	response to salt stress	GO:0009651	Biological Process	95%
Blast2go	response to zinc ion	GO:0010043	Biological Process	95%
Blast2go	response to cadmium ion	GO:0046686	Biological Process	95%
Blast2go	oxidoreductase activity, acting on CH-OH group of donors	GO:0016614	Molecular Function	95%
Blast2go	protein binding	GO:0005515	Molecular Function	95%
Blast2go	vacuolar membrane	GO:0005774	Cellular Component	95%
Blast2go	chloroplast stroma	GO:0009570	Cellular Component	95%
Blast2go	nucleus	GO:0005634	Cellular Component	95%
Blast2go	plasma membrane	GO:0005886	Cellular Component	95%
Blast2go	apoplast	GO:0048046	Cellular Component	95%

Source	InterPros	Term	Type	Identity
Sma3	L-lactate/malate dehydrogenase	IPR001557	-	-
Sma3	Lactate/malate dehydrogenase, N-terminal	IPR001236	-	-
Sma3	Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal	IPR015955	-	-
Sma3	Malate dehydrogenase, active site	IPR001252	-	-
Sma3	Malate dehydrogenase, NAD-dependent, cytosolic	IPR011274	-	-
Sma3	Malate dehydrogenase, type 2	IPR010945	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NVT9

Fln msg: atg_distance in limit (1-15): atg_distance = 13,

Fln protein: MGIPINTYWWVARQMGLVQPISFHLLALGQFSRQHFEMAKEPARILVTGAAGQIGYALVPMIARGIMLGPDQPVILHMLDIPPAAEALNGVKMELIDAAFPLLKGIVATTDPVEACSGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEQHAAPNCKVLVVANPANTNALILKEFAPSIPEKNVTCLTRLDHNRALGQISERLHVPVSNVKNVIIWGNHSSSQYPDVYHASVVTGAGEKPVRELVADDAWLDGEFITTVQQRGAAIIKARKLSSALSAASSACDHIRDWVLGTPKGTFVSMGVYSDGSYDVPPGIIFSYPVTCENGSWSIVQGLPINEFSRKKLDATANELVEEKALAYSCLN*

Protein Length: 371

Fln nts: _-_ATGGGGATACCTATAAATACGTATTGGTGGGTAGCTAGGCAGATGGGACTCGTTCAACCAATTTCATTTCATTTGCTTGCTTTGGGCCAGTTTAGCAGACAACACTTCGAAATGGCGAAGGAACCTGCTCGTATTCTTGTCACTGGAGCTGCAGGACAAATCGGTTATGCATTGGTGCCTATGATTGCTAGAGGCATAATGCTTGGCCCGGACCAACCTGTTATCTTGCACATGCTTGATATTCCTCCAGCAGCAGAGGCTCTGAATGGGGTGAAAATGGAGTTGATAGATGCTGCTTTCCCTCTTTTGAAAGGTATTGTTGCCACCACGGATCCTGTTGAAGCATGTAGTGGTGTAAACATAGCTGTCATGGTTGGTGGATTCCCCAGGAAGGAAGGTATGGAAAGGAAGGATGTTATGTCTAAAAATGTATCTATCTACAAGTCACAGGCCTCAGCATTGGAGCAACATGCTGCTCCAAATTGCAAGGTCTTGGTGGTTGCTAACCCAGCAAATACTAATGCACTGATTTTGAAAGAGTTTGCCCCATCTATTCCGGAGAAGAATGTAACATGCTTGACAAGACTCGATCACAATAGGGCTCTTGGACAGATTTCAGAGAGGCTACACGTTCCAGTCAGTAATGTAAAGAATGTTATAATTTGGGGAAATCACTCTTCAAGCCAGTATCCAGACGTTTACCATGCCTCAGTTGTAACTGGAGCAGGAGAGAAACCAGTGCGAGAGCTTGTTGCAGATGATGCTTGGTTGGATGGGGAATTTATCACCACTGTACAGCAACGTGGTGCAGCAATTATCAAGGCACGGAAGCTGTCTAGTGCACTATCTGCTGCTAGTTCCGCATGCGATCATATCCGTGATTGGGTGCTTGGTACACCTAAGGGTACATTTGTTTCAATGGGTGTATATTCCGATGGATCTTATGATGTTCCACCTGGCATTATCTTCTCATATCCTGTTACTTGTGAAAATGGGAGTTGGTCCATTGTACAAGGACTTCCCATCAACGAGTTCTCAAGGAAAAAGCTGGATGCCACTGCAAATGAACTTGTTGAGGAAAAGGCTTTGGCTTATTCTTGCCTAAATTGA___TTTATCCTTTTCAGATGCATATGTTCAAGCAACTGATTTGAATAAGTGCTATTAGTGAGATAATTGCCAAATTTGGCTTTAGGTATTGGCATGGCTGATATGAGAGATTGCTACTATCCAGATTTTAGTTTGGCTGGAAAGATTTGCTGTAACAACATCACTTCAGTGGACTGTTTTTTCCACTATATAAGCACGTGTATGCAAAATTAAATCTGTTGTTTTCAGTTTCTTAGCATTTAGCTGCCTGCCATTTCTTTAGTCTAGGAACTTTGAAGTTAATAAAATAAATACATGGTCAATCTATCATTTTAACG

Fln Alignment:

Contig5312___IPINTYWWVARQMGLVQPISFHLLALGQFSRQHFEMAKEPARILVTGAAGQIGYALVPMIARGIMLGPDQPVILHMLDIPPAAEALNGVKMELIDAAFPLLKGIVATTDPVEACSGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEQHAAPNCKVLVVANPANTNALILKEFAPSIPEKNVTCLTRLDHNRALGQISERLHVPVSNVKNVIIWGNHSSSQYPDVYHASVVTGAGEKPVRELVADDAWLDGEFITTVQQRGAAIIKARKXXXXXXXXXXXCDHIRDWVLGTPKGTFVSMGVYSDGSYDVPPGIIFSYPVTCENGSWSIVQGLPINEFSRKKLDATANELVEEKALAYSCL
A9NVT9_________________IPINRYWWQAIKT-TIQPILFQLFVFGQFSGQHFEMAKEPARVLVTGAAGQIGYALVPMIARGIMLGPDQPVILHMLDIPPAAEALNGVKMELIDAAFPLLKGIVATTDPVEACSGVNIAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEQHAAPNCKVLVVANPANTNALILKEFAPSIPEKNITCLTRLDHNRALGQISERLQVPVSDVKNVIIWGNHSSSQYPDVYHASVVTGAGEKAVRQLVADDAWLDGEFITTVQQRGAAIIKARKLSSALSAASSACDHIRDWVLGTPKGTWVSMGVYSDGSYDVPPGIIYSYPVTCENGSWSIVQGLPINEFSRKKLDATANELVEEKALAYSCL

SNPs (tot: 9)

Position	A %	C %	G %	T %	Probability
694	85			14	0.999981
857	66		33		0.997452
1285			75	25	0.996473
1286			33	33	0.997753
1295		85		14	0.993533
1296			14	85	0.993533
1297	85		14		0.993533
1298			14	85	0.993533
1316	80		20		0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain