SustainPineDB

Assembly Name: biogeco2 v2.0

UniGene Name: biog2_v2.0_unigene4789

Length: 963 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene biog2_v2.0_unigene4789 (antisense)

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene3080	13/27
SustainPine v2.0	sp_v2.0_unigene6480	3/27
SustainPine v2.0	sp_v2.0_unigene7585	2/27
SustainPine v2.0	sp_v2.0_unigene15847	8/27

Source	Descriptions	Term	Type	Identity
AutoFact	translation initiation factor 3 subunit B [Arabidopsis thaliana] sp\|Q9C5Z1.1\|EIF3B_ARATH RecName: Full=Eukaryotic translation initiation factor 3 subunit B; Short=eIF3b; AltName: Full=eIF-3-eta; AltName: Full=eIF3 p110; AltName: Full=p82 gb\|AAG53615.1\|AF2	-	-	69%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	81%
Blast2go	eukaryotic translation initiation factor 3 subunit b-like	-	-	87%

Source	GOs	Term	Type	Identity
Blast2go	translational initiation	GO:0006413	Biological Process	87%
Blast2go	DNA binding	GO:0003677	Molecular Function	87%
Blast2go	protein binding	GO:0005515	Molecular Function	87%
Blast2go	translation initiation factor activity	GO:0003743	Molecular Function	87%
Blast2go	nucleotide binding	GO:0000166	Molecular Function	87%
Blast2go	eukaryotic translation initiation factor 3 complex	GO:0005852	Cellular Component	87%
Blast2go	nucleus	GO:0005634	Cellular Component	87%
Blast2go	cytosol	GO:0005829	Cellular Component	87%

Source	InterPros	Term	Type	Identity
Sma3	Nucleotide-binding, alpha-beta plait	IPR012677	-	-
Sma3	Translation initiation factor 2A, beta propellor-like domain	IPR013979	-	-
Sma3	Translation initiation factor eIF-3b	IPR011400	-	-
Sma3	RNA recognition motif domain	IPR000504	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NWV8

Fln msg: Separated hits, possible frame ERROR between 698 and 700, your sequence is shorter than subject: 291 - 724

Fln protein: SLYQDKSTYTGFELFRIREEIFP*RFWN*RTRLTRSLHLLGSPKATGLLLSMGDGPRPDVSFYSMRSPNHVGRVFKLATIKGKQANSLYWSPIGRFIILAGLKALNGLLEFYNVDEFETMATGEHFMATDIEWDPTGRYAATAVTSVHEMENGFNIWSFNGKLLYRVSRDHFYQLLWRPRPASLLSPEKEEEISKNLRKYSKKYEAEDQDVSLLLSEQDREKRKRLQEDWQxxWVNEWKRFHEEEKLFRQELRDGEPSDEEEEYEAEEVEVEEFVDVTEEIASFGFEQLD*

Protein Length: 292

Fln nts: GATCGCTATACCAAGACAAAAGTACATACACAGGTTTTGAACTTTTCCGGATTAGGGAAGAGATATTCCCATAGAGGTTCTGGAATTAGAGAACAAGATTGACAAGATCATTGCATTTGCTTGGGAGCCCAAAGGCCACAGGTTTGCTATTATCCATGGGGGATGGACCCAGGCCAGATGTTAGTTTTTATTCAATGAGGAGCCCTAATCATGTGGGCCGGGTTTTCAAGCTGGCTACCATTAAAGGAAAACAAGCAAATTCTCTTTACTGGTCCCCCATTGGACGGTTTATTATTCTTGCAGGTCTGAAAGCTTTAAATGGTCTGCTTGAATTTTACAATGTCGATGAATTTGAGACAATGGCAACTGGTGAACATTTTATGGCAACTGATATTGAGTGGGATCCTACTGGAAGGTATGCTGCAACAGCAGTTACATCTGTTCATGAAATGGAAAATGGGTTTAACATATGGTCTTTCAATGGAAAGCTGTTGTACCGCGTTTCCAGAGATCATTTCTATCAGCTATTATGGCGTCCGAGGCCAGCCTCTCTTTTAAGCCCAGAGAAAGAGGAGGAGATTTCTAAGAATTTGAGAAAGTATAGCAAGAAGTATGAGGCTGAAGACCAGGATGTTTCATTGTTGTTGAGTGAGCAAGACAGGGAGAAACGGAAGAGATTGCAAGAGGATTGGCAGGGxxxCTGGGTCAATGAATGGAAACGTTTTCACGAGGAAGAGAAATTATTTAGGCAGGAGCTACGTGATGGAGAGCCTAGTGATGAAGAAGAGGAATATGAAGCAGAGGAAGTTGAAGTTGAAGAGTTCGTAGATGTTACTGAAGAGATTGCTTCATTTGGTTTTGAGCAGTTGGACTGATTGATTGTATAGTATGCATCTGTTTGTGCCTCCATACAGCCCTATGTTTGTGCCATTATGCAGACAGATAGCTTAGCTAGAAGATTCAGT

Fln Alignment:

Contig4789___KSTYTGFELFRIREEIFP*RFWNXXXXXXXXXXXLGSPKATGLLLSMGDGPRPDVSFYSMRSPNHVGRVFKLATIKGKQANSLYWSPIGRFIILAGLKALNGLLEFYNVDEFETMATGEHFMATDIEWDPTGRYAATAVTSVHEMENGFNIWSFNGKLLYRVSRDHFYQLLWRPRPASLLSPEKEEEISKNLRKYSKKYEAEDQDVSLLLSEQDREKRKRLQEDWQGxWVNEWKRFHEEEKLFRQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEIASFGFEQLD
A9NWV8_________________KSTYTGFELFRIKERDIPIEVLELENKNDKIIAFAWEPKGHRFAIIHGDGPRPDVSFYSMRSPNNVGRVSKLATIKGKQANSLYWSPIGRFIILAGLKALNGQLEFFNVDELETMATGEHFMATDIEWDPTGRYAATSVTVVHEMENGFNIWSFNGKLLYRIPRDHFYQLLWRPRPPSLLSPEKEEEISKNLRKYSKKYEAEDQDVSLLLSEQDREKRKRLQEDWQGxWVNEWKRLHEEEKLFRQDLRDGEPSDEEEEYEAEEVEVEELIDVTEEIASFGFEQLD

SNPs (tot: 21)

Position	A %	C %	G %	T %	Probability
683	83		16		0.994512
685			20	80	0.995493
738	85		14		0.993533
751		85	14		0.993533
752	85		14		0.993533
754			85	14	0.993533
776	14		85		0.993533
784		85		14	0.993533
801		14		85	0.993533
802		85		14	0.993533
806		33	16		1.0
846	50		50		1.0
864	75			25	0.996473
865	25			75	0.996473
867		50		50	1.0
885	50		50		1.0
897	33		66		0.997452
907		33		66	0.997452
909		66		33	0.997452
912	33			66	0.997452
915		33		66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain