SustainPineDB

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Assembly info
Unigenes
SNPs
SSRs
Descriptions
GOs
ECs
KEGGs
InterPros
miRNAs

Assembly Name: biogeco2 v2.0

UniGene Name: biog2_v2.0_unigene1086

Length: 229 nt

UniGene Fasta

>biog2_v2.0_unigene1086
ATGGGGTTGTGGGACGTTGGGCCTTTCTACAGGGACAGTCAAACTAATAGGTCCAGATGGAGAAGAGCATGTTGCATGTGCTGTTGGAACTGGTCCAGTAGATGCTGCATACAAGGCTATTGACAGCATTGTTAAGGTACCTGTGACCCTTTTGGAGTATTCACTGACTTTCTGTTACAGAAGGTATTGATGCAATTGCATCAACAGAGTCCTGGTACGAGCGGATCAC

Ace file of the UniGene biog2_v2.0_unigene1086

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Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene7891	2/3
SustainPine v2.0	sp_v2.0_unigene9490	1/3

Source	Descriptions	Term	Type	Identity
AutoFact	2-isopropylmalate synthase 2, chloroplastic n=3 Tax=Arabidopsis RepID=LEU12_ARATH	-	-	76%
FL-Next	sp=2-isopropylmalate synthase B; Solanum pennellii (Tomato) (Lycopersicon pennellii).	-	-	62%
Blast2go	2-isopropylmalate synthase	-	-	87%

Source	ECs	Term	Type	e value	Identity
Blast2go	2-isopropylmalate synthase.	EC:2.3.3.13	-	0.0	87%

Source	KEGGs	Term	Identity
Blast2go	Valine, leucine and isoleucine biosynthesis	00290	87%
Blast2go	Pyruvate metabolism	00620	87%
Blast2go	Biosynthesis of alkaloids derived from histidine and purine	01065	87%
Blast2go	Metabolic pathways	01100	87%
Blast2go	Biosynthesis of secondary metabolites	01110	87%

Source	GOs	Term	Type	Identity
Blast2go	leucine biosynthetic process	GO:0009098	Biological Process	87%
Blast2go		GO:0008415	Molecular Function	87%
Blast2go	2-isopropylmalate synthase activity	GO:0003852	Molecular Function	87%
Blast2go	chloroplast envelope	GO:0009941	Cellular Component	87%

Source	InterPros	Term	Type	Identity
Sma3	Pyruvate carboxyltransferase	IPR000891	-	-
Sma3	Alpha-isopropylmalate/homocitrate synthase, conserved site	IPR002034	-	-
Sma3	2-isopropylmalate synthase, bacterial-type	IPR005671	-	-
Sma3	2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain	IPR013709	-	-
Sma3	Aldolase-type TIM barrel	IPR013785	-	-

Full-Lengther Next Prediction

Fln status: Internal

Fln database: sp_plants

Fln subject: O04974

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 60 aas, your sequence is shorter than subject: 63 - 612

Fln protein: WGCGTLGLSTGTVKLIGPDGEEHVACAVGTGPVDAAYKAIDSIVKVPVTLLEYSLTFCYRRY*

Protein Length: 64

Fln nts: ATGGGGTTGTGGGACGTTGGGCCTTTCTACAGGGACAGTCAAACTAATAGGTCCAGATGGAGAAGAGCATGTTGCATGTGCTGTTGGAACTGGTCCAGTAGATGCTGCATACAAGGCTATTGACAGCATTGTTAAGGTACCTGTGACCCTTTTGGAGTATTCACTGACTTTCTGTTACAGAAGGTATTGATGCAATTGCATCAACAGAGTCCTGGTACGAGCGGATCAC

Fln Alignment:

Contig1086___CGTLGLSTGTVKLIGPDGEEHVACAVGTGPVDAAYKAIDSIVKVPVTLLEYSLTFCYRRY*CNCINRVLVRADH
O04974_________________CGSLGLSTATVKLIDADGREHISCSVGTGPVDAAYKAVDLIVKVPVTLLEYSMNAVTQGIDAIASTRVLIRGEN

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain