SustainPineDB

Assembly Name: biogeco3 v1.0

UniGene Name: biog3_v1.0_unigene7500

Length: 1559 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene biog3_v1.0_unigene7500 (antisense)

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene2252	27/27

Source	Descriptions	Term	Type	Identity
AutoFact	Glutamyl-tRNA reductase n=4 Tax=Coniferales RepID=B8LN13_PICSI	-	-	90%
FL-Next	sp=Glutamyl-tRNA reductase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	94%
Blast2go	glutamyl-trna reductase	-	-	86%

Source	ECs	Term	Type	e value	Identity
Blast2go	Shikimate dehydrogenase.	EC:1.1.1.25	-	0.0	86%

Source	KEGGs	Term	Type	Identity
Blast2go	Phenylalanine, tyrosine and tryptophan biosynthesis	00400		86%
Blast2go	Biosynthesis of phenylpropanoids	01061		86%
Blast2go	Biosynthesis of alkaloids derived from shikimate pathway	01063		86%
Blast2go	Biosynthesis of plant hormones	01070		86%
Blast2go	Metabolic pathways	01100		86%
Blast2go	Biosynthesis of secondary metabolites	01110		86%
Blast2go		EC:1.2.1.7-	-	86%

Source	GOs	Term	Type	Identity
Blast2go	heme biosynthetic process	GO:0006783	Biological Process	86%
Blast2go	chlorophyll biosynthetic process	GO:0015995	Biological Process	86%
Blast2go	response to light stimulus	GO:0009416	Biological Process	86%
Blast2go	oxidation-reduction process	GO:0055114	Biological Process	86%
Blast2go	shikimate 3-dehydrogenase (NADP+) activity	GO:0004764	Molecular Function	86%
Blast2go	NADP binding	GO:0050661	Molecular Function	86%
Blast2go	protein binding	GO:0005515	Molecular Function	86%
Blast2go	glutamyl-tRNA reductase activity	GO:0008883	Molecular Function	86%
Blast2go	chloroplast	GO:0009507	Cellular Component	86%

Source	InterPros	Term	Type	Identity
Sma3	Tetrapyrrole biosynthesis, glutamyl-tRNA reductase	IPR000343	-	-
Sma3	Tetrapyrrole biosynthesis, glutamyl-tRNA reductase, dimerisation domain	IPR015896	-	-
Sma3	Tetrapyrrole biosynthesis, glutamyl-tRNA reductase, N-terminal	IPR015895	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-
Sma3	Tetrapyrrole biosynthesis, glutamyl-tRNA reductase, conserved site	IPR018214	-	-
Sma3	Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase	IPR006151	-	-

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LN13

Fln msg: your sequence is shorter than subject: 429 - 537

Fln protein: PEAEWPRAIGELCSLNHIEEAAVLSTCNRMEIYVVALSWHRGIREVTEWMAKTSGIPLGELRQHLFMLRDNDASQHLFQVASGLDSLVLGEGQILAQVKQVHKVGQGVLGFERNIGGLFKHAITAGKRVRTETNIAAGAVSVSSAAVELAIMKLPKDSISFAKMLVIGAGKMGKLVIRHLIAKGCKRIVVVNRSEERVSLLQQELTGAELIYKPLTEMLNSATEADVVFTSTASETPLFLKEHAEVLPPVSAKMGGIRLFIDIAVPRNVAACVSELNSARVYNVDDLKEVVEANKEERQKKVLGAQVIIDEELRLFEAWRDSLETVPTIKKLRAYAERIRIAELEKCLSKMGDDVSKKNRKLVDDLSRGIVNKLLHGPMQHLRCDGSDSRTLSETLENMHALERMFSLESDIFVLEQKLRAKIEKAQK*

Protein Length: 430

Fln nts: CCTGAAGCGGAATGGCCACGTGCAATTGGGGAACTTTGTAGCTTGAATCATATTGAAGAGGCAGCAGTTCTTAGCACTTGCAATAGGATGGAAATTTATGTTGTTGCGTTGTCTTGGCACAGGGGGATTCGAGAGGTGACCGAATGGATGGCCAAGACAAGTGGAATACCTCTTGGAGAGCTCCGGCAGCACCTGTTCATGTTGCGTGACAATGATGCATCACAACATTTATTCCAAGTCGCATCTGGTCTTGACTCTCTTGTTCTTGGTGAAGGCCAAATCCTTGCGCAAGTTAAGCAAGTGCACAAAGTAGGCCAGGGAGTCTTGGGATTTGAGAGAAATATTGGTGGCCTCTTCAAACATGCAATCACAGCAGGAAAGCGAGTACGAACAGAAACGAATATTGCTGCAGGTGCTGTTTCAGTTAGTTCTGCTGCTGTTGAGTTGGCCATTATGAAGCTCCCAAAGGATAGTATTTCATTTGCCAAAATGCTAGTTATTGGGGCAGGCAAGATGGGAAAGCTTGTGATAAGACATTTAATTGCAAAAGGGTGCAAGAGAATTGTTGTAGTTAACAGATCTGAGGAAAGGGTTTCACTCTTACAGCAAGAACTAACTGGGGCAGAACTGATCTATAAGCCTCTTACTGAGATGCTTAATAGTGCTACAGAAGCGGATGTAGTCTTCACGAGCACAGCATCAGAAACACCTTTATTTTTGAAGGAGCACGCTGAAGTTCTTCCACCTGTGTCTGCAAAAATGGGAGGGATTCGACTTTTCATAGATATTGCTGTACCTAGGAATGTGGCTGCCTGTGTTTCAGAACTAAATTCAGCCAGAGTATATAATGTTGATGATCTCAAAGAAGTGGTTGAAGCAAACAAGGAGGAGAGACAGAAAAAGGTTCTGGGAGCTCAGGTTATTATCGATGAGGAGTTGAGGCTGTTTGAAGCATGGAGAGATTCTTTGGAGACAGTGCCTACCATAAAGAAACTGAGGGCTTATGCAGAGAGGATAAGAATTGCAGAGTTAGAGAAATGCTTGTCAAAAATGGGAGATGATGTTTCTAAGAAGAACAGGAAACTTGTGGATGACCTAAGCAGAGGCATTGTCAACAAGTTACTACATGGTCCTATGCAGCATTTGAGATGTGATGGCAGTGACAGTCGAACTTTGAGTGAGACTCTGGAAAATATGCATGCTCTTGAGCGAATGTTTAGCCTTGAATCGGACATATTTGTATTGGAGCAGAAACTTAGGGCTAAGATTGAAAAAGCTCAAAAGTAA___ATATCTGCTTGCTACCGAATGTTTTGATTTCGTGGGTACAACCATTCATGGGGGTGTAACATCTTCAACTGTATCTTTTGAATTTTCCTCTTCAGTCTTCTGCCAAGGGAAGTGTACAACAGAGTCCATTAAGATTCTAAGGCCAATAAGAAGCACTACCCTTCATTATTTTCTGTCAAGCCATCATTGATTCTCTCTCAACATTTGGAAGTTATAATAGGAAGCAATCCATGGTTGCTTCATAGATCAAATTGATTCATAGAAAAGACATT

Fln Alignment:

Contig7500___PEAEWPRAIGELCSLNHIEEAAVLSTCNRMEIYVVALSWHRGIREVTEWMAKTSGIPLGELRQHLFMLRDNDASQHLFQVASGLDSLVLGEGQILAXXXXXXXXXXXVLGFERNIGGLFKHAITAGKRVRTETNIXXXXXXXXXXXVELAIMKLPKDSISFAKMLVIGAGKMGKLVIRHLIAKGCKRIVVVNRSEERVSLLQQELTGAELIYKPLTEMLNSATEADVVFTSTASETPLFLKEHAEVLPPVSAKMGGIRLFIDIAVPRNVAACVSELNSARVYNVDDLKEVVEANKEERQKKVLGAQVIIDEELRLFEAWRDSLETVPTIKKLRAYAERIRIAELEKCLSKMGDDVSKKNRKLVDDLSRGIVNKLLHGPMQHLRCDGSDSRTLSETLENMHALERMFSLESDIFVLEQKLRAKIEKAQK
B8LN13_________________PEAEWPRAIGELCNLNHIEEAAVLSTCNRMEIYVVALSWHRGIREVTEWMSKTSGIPLGELRQHLFMLRDNDASQHLFQVSSGLDSLVLGEGQILAQVKQVHKVGQGVLGFERNIGGLFKHAITAGKRVRSETNIAAGAVSVSSAAVELAIMKLPKDGISFAKMLVIGAGKMGRLVIKHLIAKGCKRIVVVNRSEERVSLLQEELTGAELIYKPLTEMLNSAAEADVVFTSTASETPLFLKEHAEVLPPVSAELGGIRLFIDIAVPRNVGACVSEVNSTRVYNVDDLKEVVAANKEDRQKKALEAQVIIDEELRLFEAWRDSLETVPTIKKLRAYAERIRIAELEKCLSKMGDDISKKNRKLVDDLSRGIVNKLLHGPMQHLRCDGSDSRTLSETLENMHALERMFSLESDIFVLEQKIRAKIEQSQK

SNPs (tot: 10)

Position	A %	C %	G %	T %	Probability
722		20		80	0.995493
724		80		20	0.995493
894	16			83	0.994512
895	16	83			0.994512
898		40		60	1.0
1004	14			85	0.993533
1007	14	85			0.993533
1202	33	66			0.997452
1285			87	12	0.992554
1459	20	80			0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain