SustainPineDB

Assembly Name: biogeco3 v1.0

UniGene Name: biog3_v1.0_unigene4880

Length: 724 nt

UniGene Fasta

Ace file of the UniGene biog3_v1.0_unigene4880

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene3581	12/12

Source	Descriptions	Term	Type	Identity
AutoFact	Malate dehydrogenase [NADP], chloroplastic n=2 Tax=Flaveria RepID=MDHP_FLABI	-	-	84%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	82%
Blast2go	malate dehydrogenase	-	-	89%

Source	ECs	Term	Type	e value	Identity
Blast2go	Malate dehydrogenase (NADP(+)).	EC:1.1.1.82	-	0.0	89%

Source	KEGGs	Term	Identity
Blast2go	Pyruvate metabolism	00620	89%
Blast2go	Carbon fixation in photosynthetic organisms	00710	89%
Blast2go	Metabolic pathways	01100	89%

Source	GOs	Term	Type	Identity
Blast2go	carbohydrate metabolic process	GO:0005975	Biological Process	89%
Blast2go	malate metabolic process	GO:0006108	Biological Process	89%
Blast2go	oxidation-reduction process	GO:0055114	Biological Process	89%
Blast2go	binding	GO:0005488	Molecular Function	89%
Blast2go	malate dehydrogenase (NADP+) activity	GO:0046554	Molecular Function	89%
Blast2go	chloroplast	GO:0009507	Cellular Component	89%

Source	InterPros	Term	Type	Identity
Sma3	Lactate/malate dehydrogenase, N-terminal	IPR001236	-	-
Sma3	Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal	IPR015955	-	-
Sma3	Malate dehydrogenase, active site	IPR001252	-	-
Sma3	Malate dehydrogenase, NADP-dependent, plants	IPR011273	-	-
Sma3	Malate dehydrogenase, type 2	IPR010945	-	-
Sma3	NAD(P)-binding domain	IPR016040	-	-

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NX63

Fln msg: Overlapping hits, possible frame ERROR between 258 and 249, Distance to subject end: 203 aas, atg_distance in limit (1-15): atg_distance = 3, W2: There is no M at the beginning, your sequence is shorter than subject: 241 - 447

Fln protein: NVDISFTGTSQLLQRKTNVSLYRLNSSFLPQKPAKFSFNVNTEKCFPSMKVVCSVAPSQTQAPTPAPTTTLTPDGKRTDCFGxxxxxYDLKEEEKQKSWKKLIKVAVSGAAGMISNHLLFMIASGEAFGQDQPIALQLLGSERSFTALEGVAMELEDSLYPLLREVSIGVDPYEVFRDAEWALLIGAKPRGPGMERADLLDINGQIFAAQGKALNDVASPNVKVLVVGNPCNTNALICLKN

Protein Length: 242

Fln nts: A_-_ATGTCGATATCTCGTTTACTGGGACATCTCAGCTTTTGCAGAGGAAGACGAACGTCTCTTTATATCGTCTGAACAGTTCTTTCTTGCCACAAAAACCAGCCAAGTTCTCCTTCAATGTCAATACAGAAAAATGTTTCCCGTCCATGAAAGTTGTCTGCTCAGTTGCTCCAAGCCAAACTCAGGCGCCAACCCCTGCACCCACTACCACCCTGACTCCGGACGGGAAGAGAACAGATTGCTTTGGCxxxxxxxxxxxxxxxTATGATCTAAAAGAGGAAGAAAAGCAGAAGTCATGGAAGAAGCTGATAAAAGTTGCAGTATCAGGTGCTGCAGGAATGATATCAAATCATCTGCTTTTCATGATTGCCTCTGGGGAAGCTTTTGGGCAAGATCAACCAATTGCATTGCAACTGCTTGGGTCGGAGAGGTCATTCACAGCTCTAGAAGGTGTTGCAATGGAACTTGAAGATTCTTTATACCCTTTGCTGAGAGAGGTTAGCATAGGTGTGGATCCATATGAGGTTTTCCGTGATGCTGAATGGGCTCTCCTTATTGGAGCAAAGCCACGTGGGCCTGGAATGGAACGGGCTGACTTACTCGACATAAATGGACAGATTTTTGCAGCACAGGGAAAGGCTCTTAATGATGTTGCAAGCCCAAATGTAAAGGTTCTAGTAGTGGGCAATCCATGCAATACAAATGCCTTAATTTGCCTTAAGAATGC

Fln Alignment:

Contig4880___DISFTGTSQLLQRKTNVSLYRLNSSFLPQKPAKFSFNVNTEKCFPSMKVVCSVXXXXXXXXXXXXXXXXXXDGKRTDCFGIxxxTYDLKEEEKQKSWKKLIKVAVSGAAGMISNHLLFMIASGEAFGQDQPIALQLLGSERSFTALEGVAMELEDSLYPLLREVSIGVDPYEVFRDAEWALLIGAKPRGPGMERADLLDINGQIFAAQGKALNDVASPNVKVLVVGNPCNTNALICLKN
A9NX63_________________DISFSGTCQLFQRRTNVSLHNLNSSFLPQKPVKFSSNFNTEKCLPSMKVVCSVAPSQTQTPTPAPTTTITADGKRTDCYGIxxxTYDLKEEEKQKSWKKLIKVAVSGAAGMISNHLLFMIASGEAFGQDQPIALQLLGSERSFAALEGVAMELEDSLYPLLREVSIGVDPYEVFRDVEWALLIGAKPRGPGMERADLLDINGQIFAAQGKALDAVASPNVKVLVVGNPCNTNALICLKN

SNPs (tot: 7)

Position	A %	C %	G %	T %	Probability
433	11	88			0.923564
436			11	88	0.923564
437		88		11	0.923564
590	25		75		0.996473
591			75	25	0.996473
592			75	25	0.996473
603		66		33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain