SustainPineDB

Assembly Name: biogeco3 v1.0

UniGene Name: biog3_v1.0_unigene3274

Length: 1069 nt

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene biog3_v1.0_unigene3274 (antisense)

Annotations

Parent Assembly	Parent UniGene	Reads (related/total)
SustainPine v2.0	sp_v2.0_unigene2420	41/42

Source	Descriptions	Term	Type	Identity
AutoFact	Alpha-amylase n=2 Tax=Hordeum vulgare subsp. vulgare RepID=C3W8N1_HORVD	-	-	71%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	46%
Blast2go	alpha-amylase-like 2	-	-	84%

Source	ECs	Term	Type	e value	Identity
Blast2go		EC:3.2.1.6-	-	0.0	84%
Blast2go	Alpha-amylase.	EC:3.2.1.1	-	0.0	84%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Starch and sucrose metabolism	00500		0.0	84%
Blast2go	Metabolic pathways	01100		0.0	84%

Source	GOs	Term	Type	Identity
Blast2go	carbohydrate metabolic process	GO:0005975	Biological Process	84%
Blast2go	glucan 1,4-alpha-maltotetraohydrolase activity	GO:0033910	Molecular Function	84%
Blast2go	alpha-amylase activity	GO:0004556	Molecular Function	84%
Blast2go	calcium ion binding	GO:0005509	Molecular Function	84%
Blast2go	extracellular region	GO:0005576	Cellular Component	84%

Source	InterPros	Term	Type	Identity
Sma3	Phosphotransferase system, HPr histidine phosphorylation site	IPR001020	-	-
Sma3	Phosphoribosylglycinamide formyltransferase, active site	IPR001555	-	-
Sma3	Pentatricopeptide repeat	IPR002885	-	-
Sma3	Glycoside hydrolase, family 13	IPR006046	-	-
Sma3	Glycosyl hydrolase, family 13, catalytic domain	IPR006047	-	-
Sma3	Glycosyl hydrolase, family 13, subfamily, catalytic domain	IPR006589	-	-
Sma3	Alpha-amylase, C-terminal beta-sheet	IPR012850	-	-
Sma3	Alpha-amylase, plant	IPR013775	-	-
Sma3	Glycosyl hydrolase, family 13, all-beta	IPR013780	-	-
Sma3	Glycoside hydrolase, subgroup, catalytic domain	IPR013781	-	-

Full-Lengther Next Prediction

Fln status: Internal

Fln database: coniferopsida.fasta

Fln subject: A9NUX3

Fln msg: Unexpected stop codon in the beginning of your sequence, Distance to subject end: 19 aas, your sequence is shorter than subject: 356 - 448

Fln protein: M*KIPHIAKAGFTSIWLPPAFDSLAPQGYLPRNIYSLNSAYGSENQLKSLLVTTENNNVRAMADIVINHRVGSTQGCGGLYNRYDGLPLPWDERAVTSCTGGLGNLSTGENFDGVPNIDHTQDFVRKDIKEWLIWLRKSVGFQDFRFDFAKGFAAKFVREYVEASDPMFAVGEYWVSCNYEPSSSQLAYNQDSHRQKIIDWINGTGGVSAAFDFTTKGILQEAVRGEWWRLRDCKGKPSGVLGWWPSRAVTFVENHDTGSTQAHWVFPSDHIMEGYAYILTHPGIPTVFYDHFFDWGSSIQDQIIKLMNIRRTQDLHSRSAIRILEANSSVYAAIIGEKVCMKIGVGSWCPNGKDI

Protein Length: 357

Fln nts: AATGTAAAAAATCCCGCACATTGCCAAAGCAGGATTTACATCTATATGGCTGCCGCCTGCTTTTGATTCACTTGCACCCCAAGGTTATTTGCCCCGAAACATTTATTCCCTCAACTCTGCATATGGTTCAGAAAATCAATTGAAAAGCCTACTTGTGACAACGGAAAACAACAATGTGAGAGCAATGGCTGACATAGTTATCAATCATCGTGTGGGAAGCACTCAAGGATGTGGAGGCCTTTATAATCGCTATGATGGCTTGCCACTACCTTGGGATGAACGTGCTGTCACTAGTTGTACTGGTGGACTTGGAAACTTGAGCACAGGTGAGAATTTTGATGGAGTACCAAACATTGATCACACCCAAGATTTTGTACGCAAAGACATTAAAGAGTGGTTAATATGGCTTCGGAAAAGTGTTGGTTTCCAAGATTTTCGATTTGATTTTGCTAAGGGTTTTGCAGCAAAATTTGTGAGAGAGTATGTTGAAGCATCAGACCCCATGTTTGCTGTAGGGGAATATTGGGTGTCTTGCAATTATGAGCCTTCTAGCAGTCAACTGGCTTATAACCAAGATAGCCATAGACAGAAAATAATAGACTGGATTAACGGTACTGGTGGTGTGTCTGCGGCATTTGATTTTACAACAAAAGGCATTCTTCAGGAAGCAGTCAGAGGAGAGTGGTGGCGTTTACGTGATTGCAAAGGAAAACCCTCTGGAGTGCTTGGCTGGTGGCCATCAAGGGCTGTAACTTTTGTTGAAAACCATGACACTGGGTCAACACAGGCTCACTGGGTTTTTCCAAGTGATCATATTATGGAGGGCTATGCTTACATCTTGACACACCCTGGAATACCAACAGTCTTCTATGACCATTTTTTTGATTGGGGAAGCTCAATACAAGACCAGATTATAAAGCTGATGAACATTCGAAGAACACAAGATTTGCACAGCCGTTCTGCAATCAGGATTTTAGAGGCCAATTCCAGTGTTTATGCTGCAATTATTGGAGAAAAAGTTTGCATGAAGATTGGAGTTGGATCATGGTGTCCAAATGGCAAAGACATT

Fln Alignment:

Contig3274___AKAGFTSIWLPPAFDSLAPQGYLPRNIYSLNSA-YGSENQLKSLLVTTENNNVRAMADIVINHRVGSTQGCGGLYNRYDGLP----LPWDERAVT------SCTGGLGNLSTGENFDGVPNIDHTQDFVRKDIKEWLIWLRKSVGFQDFRFDFAKGFAAKFVREYVEASDPMFAVGEYWVSCNYEPSSSQLAYNQDSHRQKIIDWINGTGGVSAAFDFTTKGILQEAVRG-EWWRLRDCKGKPSGVLGWWPSRAVTFVENHDTGSTQAHWVFPSDHIMEGYAYILTHPGIPTVFYDHFFDWGSSIQDQIIKLMNIRRTQDLHSRSAIRILEANSSVYAAIIGEKVCMKIG----VGSWCP
A9NUX3_________________ADAGITDVWFPPPSQSLAPEGYLPQRLYDLNSSKYGSEQELRNAVDAFHQKGIGCVGDMVINHRSGTKQDDKGMWCVFEGGKGDGRLDWGPWAVTVNDKPFAC--GSGQADTGGDYAAAPDIDHTNPKIQQDLSEWMNWLKSNVGFDGWRFDFAKGYAGNLLAVYLKNTSPKFAVGEVWDDMSY--SDGRLAYDQGAHRQRLVDWVHSTGDGAAAFDFTTKGILQEAVKSNELWRLKDSNGKPAGMIGVLPQKAVTFIDNHDTGSTQNIWPFPSDKLLQGYAYILTHPGIPTIFYDHFVD--GNLKKEIQNLSAIRKRNNINANSNCRIITAESDLYMAAIDEKIVVKIGSRYDVGNLAP

SNPs (tot: 29)

Position	A %	C %	G %	T %	Probability
72	16	83			0.994512
75		83		16	0.994512
136	16			83	0.994512
137	16	83			0.994512
213	20			80	0.995493
214	20			80	0.995493
215			80	20	0.995493
251	16			83	0.994512
252	16	83			0.994512
253		83		16	0.994512
254	83		16		0.994512
299	25		75		1.0
326	12		87		0.992554
327	12	87			0.992554
328		87		12	0.992554
329		87	12		0.992554
606	14			85	0.993533
607		16		83	0.994512
608	16		83		0.994512
609		83		16	0.994512
642		87		12	0.992554
722	10			90	0.90298
727	10			90	0.90298
729		90	10		0.90298
881	10			90	0.90298
884			90	10	0.90298
885		90		10	0.90298
974		25	75		0.996473
975	25			75	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain